LOCUS       NZ_OM144976             5721 bp    DNA     circular CON 25-SEP-2023
DEFINITION  Klebsiella pneumoniae strain F4 plasmid pC, complete sequence.
ACCESSION   NZ_OM144976
VERSION     NZ_OM144976.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2022) Department of Central Laboratory, Municipal
            Hospital Affiliated to Taizhou University, No.381-1,Zhongshan East
            Road,Jiaojiang, Taizhou, Zhejiang 318000, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.5
            Sequencing Technology             :: nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/25/2023 00:36:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 4
            CDSs (with protein)               :: 4
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OM144976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F4"
                     /db_xref="taxon:573"
                     /plasmid="pC"
                     /geo_loc_name="China: Taizhou"
                     /collection_date="2019"
     gene            join(5409..5721,1..140)
                     /locus_tag="N4J57_RS00005"
     CDS             join(5409..5721,1..140)
                     /locus_tag="N4J57_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="WP_003833285.1"
                     /translation="MNKLLSLVVSLALLPGAVFAASTPDCVKVNKAQIEALFDKWNESL
                     KTGNAQTVSENYLSDAVLLPTVSNKARLTDAERVDYFEHFLAKKPTGKIDMRTIRLGCN
                     KAIDTGTYTFTFADKSTVSARYTFTYAWDGKEWKISTHHSSAMPEG"
                     /name="SgcJ/EcaC family oxidoreductase"
     gene            complement(182..826)
                     /locus_tag="N4J57_RS00010"
     CDS             complement(182..826)
                     /locus_tag="N4J57_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB52"
                     /protein_id="WP_063866158.1"
                     /translation="MALALVGEKINRNRFTGEKIENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNASALGIEISHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGAQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRRVDLQGVKLDNYQASLLMERLGIAIIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB52"
     gene            complement(922..>1128)
                     /locus_tag="N4J57_RS00015"
                     /pseudo=""
     CDS             complement(922..>1128)
                     /locus_tag="N4J57_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833280.1"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            <1120..1323
                     /locus_tag="N4J57_RS00020"
                     /pseudo=""
     CDS             <1120..1323
                     /locus_tag="N4J57_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA];
                     GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /name="SMR family transporter"
     gene            1317..2156
                     /gene="sul1"
                     /locus_tag="N4J57_RS00025"
     ARG             1318..2153
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2561..4102
                     /locus_tag="N4J57_RS00030"
     CDS             2561..4102
                     /locus_tag="N4J57_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISCR1 family transposase"
                     /protein_id="WP_000050481.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASLE
                     AQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGARRM
                     AESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIKKAG
                     YTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYDELNT
                     LAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGPQQGRK
                     VFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPAVSEKRL
                     ALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVFAPNSKHR
                     VQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVCEHCGGHVK
                     VIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQSRLFD"
                     /name="IS91-like element ISCR1 family transposase"
     gene            complement(<4335..4874)
                     /locus_tag="N4J57_RS00035"
                     /pseudo=""
     CDS             complement(<4335..4874)
                     /locus_tag="N4J57_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /name="ABC transporter substrate-binding protein"
     ARG             complement(186..826)
                     /name="qnrB52"
                     /gene="qnrB52"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB52"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(183..826)
                     /name="QnrB2"
                     /gene="QnrB2"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 aggcgataga caccggtacc tacacattta ccttcgcgga caaatcaacg gtttcagccc
       61 gctacacatt cacttatgca tgggacggta aagagtggaa aatttccaca caccactctt
      121 cagcgatgcc tgaagggtaa aaagctgtgc agggggcgga atattcaccg ctccctgaag
      181 actagccaat aatcgcgatg ccaagacgtt ccatgagcaa cgatgcctgg tagttgtcca
      241 acttaacgcc ttgtaaatca acgcgccgaa tatctaagtc acccaactcc gaattggtca
      301 gatcgcaatg tgtgaagttt gctgctcgcc agtcgaaagt cgaaaactcg ccgccggaga
      361 gatctgaacc actgaacgtc gcgcccagta cctgggcccc catccaacgg ttttcccaca
      421 gctcacactt ttccaacacg actttcgaaa aattggcgta gcttagattt gtgttagtga
      481 tatatgcact gcaaaaccag gtgcgagtag tgatcatatt cataaaactt gcgccgcgaa
      541 aatctgcgcc ttgcgcacga cagtggctaa tttcaatgcc aagcgcactg gcattgcgaa
      601 aatccgccat ggataaatca cagcttttaa aaatggcatc tttcagcatc gcacgactaa
      661 aattgcaccc tttctggctt tcacgatcat agaactgaca gccgataaat tcagtgccgc
      721 tcaggtcggc acctgaaaaa tcacagttaa aaaatgtact attttcaatt ttctcaccgg
      781 tgaagcggtt tctgttaatt ttttcgccaa cgagtgccag agccatattt tgtgcctgtt
      841 tttttataca gtaatggcgt catggtaaac cctgatgagg ttatgcgtca aatccgccaa
      901 tataacatct gcaaatgtgc gttaaatctg gtgttttttc agcaaagcgc gaagctgatg
      961 gtaagtcaga cccagtaatt cagcggcttt tttctggtta aattttgcct gctgtaagct
     1021 ggtttgtaga aagtctttct cttgctgctg ctggaattca cgcagatcca gcggtaaccc
     1081 tacagacatc ggtttagttt ccggcgcctg cggctgcgtc tggttctgaa atccatccct
     1141 gtcggtgttg cttatgcagt ctggtcggga ctcggcgtcg tcataattac agccattgcc
     1201 tggttgcttc atgggcaaaa gcttgatgcg tggggctttg taggtatggg gctcataatt
     1261 gctgcctttt tgctcgcccg atccccatcg tggaagtcgc tgcggaggcc gacgccatgg
     1321 tgacggtgtt cggcattctg aatctcaccg aggactcctt cttcgatgag agccggcggc
     1381 tagaccccgc cggcgctgtc accgcggcga tcgaaatgct gcgagtcgga tcagacgtcg
     1441 tggatgtcgg accggccgcc agccatccgg acgcgaggcc tgtatcgccg gccgatgaga
     1501 tcagacgtat tgcgccgctc ttagacgccc tgtccgatca gatgcaccgt gtttcaatcg
     1561 acagcttcca accggaaacc cagcgctatg cgctcaagcg cggcgtgggc tacctgaacg
     1621 atatccaagg atttcctgac cctgcgctct atcccgatat tgctgaggcg gactgcaggc
     1681 tggtggttat gcactcagcg cagcgggatg gcatcgccac ccgcaccggt caccttcgac
     1741 ccgaagacgc gctcgacgag attgtgcggt tcttcgaggc gcgggtttcc gccttgcgac
     1801 ggagcggggt cgctgccgac cggctcatcc tcgatccggg gatgggattt ttcttgagcc
     1861 ccgcaccgga aacatcgctg cacgtgctgt cgaaccttca aaagctgaag tcggcgttgg
     1921 ggcttccgct attggtctcg gtgtcgcgga aatccttctt gggcgccacc gttggccttc
     1981 ctgtaaagga tctgggtcca gcgagccttg cggcggaact tcacgcgatc ggcaatggcg
     2041 ctgactacgt ccgcacccac gcgcctggag atctgcgaag cgcaatcacc ttctcggaaa
     2101 ccctcgcgaa atttcgcagt cgcgacgcca gagaccgagg gttagatcat gcctagcatt
     2161 caccttccgg ccgcccgcta aatatctcct tttgggttgt taataaaaca tccaataagt
     2221 tgactgtgcg tgaaaaagaa agttttgtgt gatggcgttg aagatcgcac cgttaagctc
     2281 ttatgtggga tggtgcagag ctcgacgact accgataaaa cgcaaccgcc gcaaacagac
     2341 aagaaaaagc cccaactgat aacagttggg gcttcagtat tgtgattggt ggagcaatag
     2401 caccctgaac ccaaaacctt ctcgctcaac cggtagtggc tgataacaac tcgtgagggc
     2461 tattgcgggt taagcattta gcgatgtcta gggccagact ggacgtctga acgcaagccg
     2521 ctgatactgt acataaccac agtatcagcg gaggataccc atgtcgctgg caaggaacgc
     2581 cacggcgagt caatcgccca ctcaaacaaa cggttacgaa cgccaccaac ccgaccagac
     2641 gctgctctac cagctggttg agcagcacta cccagccttc aaagcctcac tcgaagccca
     2701 aggtcaacac ctgcctcgct acatccaaca agaattcaac gacctcctcc aatgtggccg
     2761 tctggagtat ggtttcatgc gggttcgctg cgaggattgt catcacgagc gtctggtcgc
     2821 cttcagctgt aaacgacgcg gcttttgccc tagctgcggt gcccgccgga tggccgagag
     2881 tgcggcgctg ctgatagacg aagtcttccc caaggagccc attcgccagt gggtgctcag
     2941 ctttcctttc cagctacgct ttttgctggc tcgccatccc cagctgatgg gccaggtctt
     3001 gagtatcgtc tatcgtacac tctcaactca tctgatcaaa aaagccggtt acaccaaagc
     3061 ctctgcacaa actggctcag tgactcttat ccaacgcttt ggctccgcgc taaatctcaa
     3121 tgtccactac cacatgctgt ttctcgatgg tgtctatgcc gaagatgact atggcaagca
     3181 acgcttccat cgtgtcaagg cacccactta cgatgagctg aatacgctcg ctcacaccct
     3241 cagccatcgc atcgctcgct gcatggaaaa gcgtgggatt ttggagcgtg atgccgagaa
     3301 tacgtggttg acactggaag agggcgaaga cgatacgctg actcaattac atggtgcttc
     3361 ggttacgtat cgcattgccg tcggccccca gcaagggcgc aaagtcttca ccctgcaaac
     3421 cttgccaggg cgtgaggata aagccgactc aagcagtcga gtagccaacc atgctggttt
     3481 ctcgctacac gccggtgtga tggccgaagc gcatcagcgg gataagcttg agcgcttgtg
     3541 tcgctacatt agtcggccag cggtttcaga aaaacgtctg gcattaaccg ccaatgggca
     3601 ggtgcgttac gagctcaaaa ctccgtaccg caatggcacc acccatgtga tcttcgagcc
     3661 gctggacttc atcgccaaac tcgctgcgtt ggtacctaag ccgcgagtca acctcacacg
     3721 cttccacggc gtctttgcac cgaacagcaa acaccgagtt caagtaacac ccgccaagcg
     3781 gggcaagaag cccgacaaat cggaaggtct cgatactaac tggcgtgaca agagtcctgc
     3841 agagcgccac cgcgccatga cctggatgca acgcctcaag cgagtcttca atattgatat
     3901 tgaagtctgc gaacactgcg gcggtcacgt caaagtgatt gccagcatcg aagatccgaa
     3961 ggtcattgag cagattctca agcatctgaa acagaaaaca gccaaggcga atgccgccaa
     4021 gcagcgtgag ctgccaccag aacgagcgcc gccactgact cccagcctgt tcgatccatc
     4081 acagagtcgt ctctttgact gacgacccca aatccaacac tgctcaacac tgccaacttt
     4141 taaacggggc ggtggggcag tttgtatctc tcgagctatc aggctagaga ttttaccgcc
     4201 aaatcgaacc ttattagagc ggtttaggct ggaccggcag ttaaaattgg ggcttgagcg
     4261 gtaaacgagt gagggaattt caggtaagat acttcggatg aggagcaaaa aggtggttta
     4321 tacttcctat accccataaa ggaggcatcc ggttgcgtta agcggaattc aacggtgttg
     4381 ttgtccagtt tgcgcacgct ttggacgtta tcggcaaact gcaggctgtc gaagtagggg
     4441 aaactgctgc cgttcacatt gtgccacgga tggtggcgat caaagatgcg ctcgaaggta
     4501 aacaccacat cgtctgcatt cagtttgcgg gtgggggtga accaggccgt cttctgaaac
     4561 tgaacgtcac gacgcagatg gaagcggtag gttgcgccgt tatccagcac ctcccagctt
     4621 tcagcaagct caggcaccag ccgataggtg tagggatcta catcaagcag tcggtcatac
     4681 aattgggccg ctaacgtatc gacgatgaga ccgctgctcg ttttttgtgg gttgaacgta
     4741 ttgacttgcc cgctgacgca atagacaaac ccactgtcac ggatatcagc gtgcggagct
     4801 tgctcaggcg cagcagcagc ctgaccactc agaaatccag ccatcacgat cagagatgat
     4861 aaaaccaggc gcataatttt aatggattat ataaagaggc tatcttacta atacttaatg
     4921 acatttgcca ttaccgtttg tttttgggag taacaggctc gataagcgcg agttcaacac
     4981 cgcgacatgg tcaaaattca tacctgctat ccactttgaa tctactctgt taactatctt
     5041 agcattttca tggcccatct gacttgctat aaacgtgggg gcgtacctgt cgttgtttac
     5101 tgattgatat cagtgtgtgc tcggaagatg gagtatggca tcgataacga agtgtggagc
     5161 gagcatttag agtgtcacta tcaatgctga actataatca ttcaggaagc agtacacgtt
     5221 atgtgagttg taatagctaa agtatctata attatttcaa tatgttaatt ccattttttc
     5281 ttcatggcat cttaatcttc tcacccattt atggtgatga tttccattgg ttattaaatg
     5341 cattttggct ttgatcctgg ttatgaacgg ttttctgttg ccatttaact tctaatggaa
     5401 tgaattgtat gaataaatta ctgagccttg ttgtttcact tgccctattg cctggagccg
     5461 tttttgcagc gtcgactcca gactgtgtga aggtgaataa agcgcaaatc gaagcgctat
     5521 ttgataagtg gaacgagtca ttgaaaacag gtaacgcgca gacggtatct gaaaattatc
     5581 ttagcgatgc ggtattgttg cctactgtat ccaataaagc gcgtttaacg gacgctgaac
     5641 gtgttgatta tttcgagcat tttcttgcga aaaagccgac cgggaaaatt gacatgcgta
     5701 ccattcgcct ggggtgtaat a
//
