LOCUS       NZ_OM001479            11970 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Escherichia coli strain CR-HvKP5TC-5 plasmid pCR-HvKP5TC-5_p4,
            complete sequence.
ACCESSION   NZ_OM001479
VERSION     NZ_OM001479.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Transmission of pLVPK-like virulence plasmid in Klebsiella
            pneumoniae mediated by an Incl1 conjugative helper plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2021) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Tat Chee Avenue, Hong Kong,
            Kowloon 999999, Hong Kong
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.8
            Sequencing Technology        :: Illumina; Oxford Nanopore MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_022869525.1-RS_2024_06_02
            Annotation Date              :: 06/02/2024 03:31:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 14
            CDSs (total)                 :: 14
            Genes (coding)               :: 14
            CDSs (with protein)          :: 14
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OM001479.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP5TC-5"
                     /db_xref="taxon:562"
                     /plasmid="pCR-HvKP5TC-5_p4"
                     /geo_loc_name="China"
                     /lat_lon="22.32 N 114.18 E"
                     /collection_date="2021"
                     /identified_by="Y.XM"
     gene            7..537
                     /locus_tag="MW703_RS00005"
     CDS             7..537
                     /locus_tag="MW703_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(564..743)
                     /locus_tag="MW703_RS00010"
     CDS             complement(564..743)
                     /locus_tag="MW703_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(769..1197)
                     /locus_tag="MW703_RS00015"
     CDS             complement(769..1197)
                     /locus_tag="MW703_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(1361..1675)
                     /locus_tag="MW703_RS00020"
     CDS             complement(1361..1675)
                     /locus_tag="MW703_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1663..1941)
                     /locus_tag="MW703_RS00025"
     CDS             complement(1663..1941)
                     /locus_tag="MW703_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(2116..2481)
                     /locus_tag="MW703_RS00030"
     CDS             complement(2116..2481)
                     /locus_tag="MW703_RS00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(2478..2849)
                     /gene="tolA"
                     /locus_tag="MW703_RS00035"
     CDS             complement(2478..2849)
                     /gene="tolA"
                     /locus_tag="MW703_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(3123..3368)
                     /locus_tag="MW703_RS00040"
     CDS             complement(3123..3368)
                     /locus_tag="MW703_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4013..5500
                     /gene="ltrA"
                     /locus_tag="MW703_RS00045"
     CDS             4013..5500
                     /gene="ltrA"
                     /locus_tag="MW703_RS00045"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            5605..7290
                     /locus_tag="MW703_RS00050"
     CDS             5605..7290
                     /locus_tag="MW703_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7300..7557
                     /locus_tag="MW703_RS00055"
     CDS             7300..7557
                     /locus_tag="MW703_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7642..7791
                     /locus_tag="MW703_RS00060"
     CDS             7642..7791
                     /locus_tag="MW703_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            9275..11239
                     /locus_tag="MW703_RS00065"
     CDS             9275..11239
                     /locus_tag="MW703_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            11239..11970
                     /gene="mobC"
                     /locus_tag="MW703_RS00070"
     CDS             11239..11970
                     /gene="mobC"
                     /locus_tag="MW703_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             605..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7895..8473)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11240..11967
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gggattatgg cgctgtacat ggtatttatt gaggacgtgg aagacaaaga cggcgagcgc
       61 tgcattatgc caaccagcga gcatctggaa gacatggtgg tgctcgcctg gcagaccggg
      121 gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg
      181 atgtatgacg agcggctcaa cagcgacgaa aaagccacgt actttttcac cggtgaaaat
      241 ccgcaatggg aacgggacaa acccacagga atgaagctgt gtgattacct ctatcgaaag
      301 gtggagcggt tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg
      361 gagcagatta acgcgatgat tgaggtggtc agcgagcggc acggggaaag cgcggtgaac
      421 gagaagctga aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggtg
      481 cctgaactgc cggaggacaa acccgcccgc cggtggtggc cgttcggcaa aaaataacgc
      541 cagcaccggc atggccggtg ctgttattct tccccgagct tatcccgcat cacctgccag
      601 agtgtttccg catcctcgtg aagccgttcg cacatgtcgg ccggggtatc catgtccagc
      661 tcgttcagct tttccagcaa cagcagcgac tgatccttaa tgaatttagc catattgagg
      721 gcagtttttt cctgcttcgt catgttctcc attcccttcg gtaagttctc agctaaacca
      781 gccggaccag aagcgttttt tcttctgtgt cagttctgtt gtcaatctgt ccacttcggc
      841 ctggagtttc cggcgctcat catcgtgctg gcggcgttct tcttcccggg atgttttatc
      901 ttccagcatc agggtgacag cctgccggag atcggccagt tccttttgca ttgccagaag
      961 ctgttcctgt gggaaatttt tttgtgtatt ttcacggtgt gaaatgccgg gaatttgttt
     1021 ctcagggata ttcacatttc cgtatacacg gatcagttct gacacatcca cgacagggtt
     1081 gtttttcccg tcacgtgaca cggtaacctt tccctgtttt acatgattgt atagcgttct
     1141 tctggtaatg cccgcggctt gtgctgcctg tgaaaggttg agcaatgttt tcgccactgt
     1201 atacccctga ttttatcggg tgtgaaaggt gtgtaatttc tcacggtaaa tgtatcgctg
     1261 aattttaccc gtttttccgt tcaggatgtg gttttctgac gcgctgcgct tgtcaaaccg
     1321 gcgacggcca cgcaatgaat tgcgcccccg tcatcggtta ttagaggctg tctgcccccg
     1381 gatacccgga ttctttctga tgcctgacgg tgacgatcac aatctggtcc atttcccggt
     1441 cgtggcggta gagcatcaca tagccactgt cgccaaaccc gatcaccagt tccttatatt
     1501 ccagcggcag aaacggcacc gggcggccaa tgtctggcaa tgttttcagt tgctggatag
     1561 cccggacgat cacctcacct ccttttctgg ctgccagccg atttttggtt ttgagaaagt
     1621 cctgaaggcg ttgtaaatcg cgctgtgcca gcgcggaaat cgttacctgt ggcatggcgg
     1681 agcatcctgc tcgttctcag ttccccaggt acttatccag gcttccgctt cttccgcagt
     1741 aaggtgcaga cccgtttcct gatagtgctg ccatgcggcc tgtccgtccc gcaggtactg
     1801 gctgcgcttc tcttcccggt cgatgtattc ggtgattgcc tccagcatta gcgcgtgcgc
     1861 tgagcggtgc cggtcatcgg ccagcgtttt cagccggtcc ttaagttctt catcgagcct
     1921 gatggatgtt gctgatgaca taaaagcccc tttgtagtca tatgtattac acatgactac
     1981 tttaatccgt ggcggcgcta tttgccagaa atacggcttt tgctgcccat ttttcagctt
     2041 cgcttccact aatcagggct gctacaggct cactcatcat ccaggactat tgcagcctgc
     2101 gggttaaagg acctgttact ggcggcaacc atcatcattg agcttttggg tggtgactgg
     2161 tcgcggatcc tgtaagccgt catttttcag actgaattca aaatttataa cctgtcgcat
     2221 tgccgggtgc cctgcgggat atagccagtg cctcattgcc gtcagcgttt cccgaaaaaa
     2281 aaggtgacag ccggtaagcg acacgttgtc cactcgtcca tcgtccagaa tatcaaacgt
     2341 ggcggtcacc tgaccttcga tgcgttttgc tgcggcctgt accgggtagg ttggctgcag
     2401 tgtggttacg ggttttagtg gtatcgcatg taccggagtg aggaacatgg ccgccagcac
     2461 tgtcagagta aaagctttca tggtctgaaa tccagagggg cgtttttgaa tatttgccag
     2521 gtattttcat ccggtgccgg cggaattttg gcgtgggtaa cggctgatat tgctgcttta
     2581 cagagtttcg gatctcctcc ttcagcaatg gcgctgttca gagtgccatc acgttgtatg
     2641 gatatccgca cggtgcacat ttttcccgca taggcctctg gttcaaacat ctgcatttca
     2701 acagcggtct tgatggccgc agcgtagcta ctgattttag atgacttatc cagagtataa
     2761 attttcggcg gctgggtggt ggaatgctta caggccgtca ggagtaaggg gctgcacaat
     2821 gccagcagga taaagggtat taaacgcatc gggttgatga cctgtcagta aaaaatgtta
     2881 catgtatatt tgtcactctt tacggccacg ttcatttaca taaacacggc ttatcgtatc
     2941 gtttttacac caaacggccc gccatgcagt tgcgtgcata ggcatgcagc gggttacaat
     3001 ttaccgggcg cgtttccggc ggttttccgg gtggtttgtt gcctgtttta tcccgtagcc
     3061 gccggaaacg ccctcagacc gtctgagcgg tgcgggcaat gcgacgttat ggtaaatacc
     3121 ccttaccggc ggcgctgtgg ctgccaggtg aaggtggttt ccgtagcctc gtatttctcc
     3181 cgacgcattt cctgcgcccg cctggcttca cgttcgtcct gaatggcacg aagcaccctt
     3241 tcccgcacct gttgttttac cgcctttggc agcgttttgt tccctttcag gcccatggcc
     3301 agtgcctgag cgacaaaggc gtccgccagg cgcttttctt cagcgggagt cagtttcagg
     3361 tgcgtcatag tggcctccgg gcgtctcagg tttcccgaac agtttaaccg caggtcctct
     3421 cctcatgcca gttagctttc agatgtgtct ataccatctg aaatgtaagt gtgatgaatg
     3481 tcttaaataa taaaatttgt tgccatgaga aattttcggg aaaagacgcg atctgcttac
     3541 aggtggctcc gggcgctttt ttgtgcctga agatgagaac taatctcata tattgacacc
     3601 tgaaaacggg aggagtaagg taataattat actgtgtata tgtacagtgt gcgccgggag
     3661 accggttaga gatcaagggg tgaaagtccc cgaccattga aggaccagca atccacaagg
     3721 tccccgagtc atgcgttgca taccgcgagg tatggggcga agcgttgaca ggggtgttga
     3781 caggccagcc attgagccac gaaatgtata ttaaattacc gggtgccgac gttgtactgt
     3841 taacggaagg caacatcata gggtgcgata ctgcgagtgc cacatggacc cggcggggtc
     3901 tgagaccctg gcatgtcaat acgatctcta cgcgggaacc gggagatctc ccctctgacc
     3961 atctgccagt gtcggagatg gcccgcaccg ggaagacgag gagtcatagc cggtgatgta
     4021 cggagaggag aagtcggact cgctcatagt agcggcgaag caggcgaaca acccgaaagg
     4081 agcggagtca gtggagcgaa ggagcggggc caaggggaac gcggaacagc cacacatgcg
     4141 ccggacacag agccgggaaa gcatgtcaca gaggctgtca cgcgtgcggg aagctgcgaa
     4201 gcagcggaag aaagaacggt ttacagcatt gttccacctg ctgacagtcg aagcactgga
     4261 agccgcattc ctctccctga gcaggaaagc ggccgccgga gtggatggca tcaggtggat
     4321 ggactacgcc ggaaacatga agaacaacat aacagatctg caccggaggc tacatcaggg
     4381 cagctacagg gcgcagcccg gcaggcgtca ctacatccca aaagcggatg gaaaacaacg
     4441 cccgctcggc atcgcctcgc tggaggacaa gatcgtccag tatgcgctgg tgaaaatcct
     4501 gaacgcagtc tatgaaaacg actttatggg gttctcatac gggttcagac ccgggcgaag
     4561 ccagcacgat gcactggacg cactggccac agggctggta cgcactaacg taaactgggt
     4621 actggatgcc gacatcagtc agttcttcga cagggtgagc cacgaatggc tgatcaggtt
     4681 cacagagcat cggatcggcg accggagggt aatcaggctc atacgtaagt ggctcacagc
     4741 cgggacgtcg gaggagggtc aatggcgagc aacggaggaa ggcaccccac agggtgcggt
     4801 catctcaccg ctgctggcaa acatatacct ccactacgtc ttcgatctgt gggcgcatca
     4861 gtggcgacgt cgctatgcca caggcaatgt ggtaatggtc agatacgccg atgacatcgt
     4921 catcgggttc gacaaacgat acgatgcccg gcgcttccgt atagccatgc agcgcagact
     4981 gagggagttc ggactcacgg ttcacccgga gaaaacccgt ctgatggagt tcggccgctt
     5041 cgctgccgaa aaccgtgcca tcaggggaaa aggcaaacca gaaacgttca acttcctcgg
     5101 gttcacgcac atcagcggga aagatcgcaa cggcaggttc atgctgatac gaaagacccg
     5161 ccgggatcgg atgacggcaa ctctgaaagc catcaaagac ggtctgcgaa ggcgctggca
     5221 ttactcaatc cccgaacagg gaaaatggct caggagagtg gttcagggat acctgaacta
     5281 tcactcggta ccgggcaact tccccaccat gcagaagttc aggacacacg taacaaacct
     5341 ctggcgccgg gcgctcaggc gcaggagcca gaaggatgat acgacctgga cgaaagcaaa
     5401 caaactggca gccgcatggc taccaagggt tcgggttctt catccatggc ctgtggagcg
     5461 gttcaccgcc agacacccga ggcaggagcc cggtgcgtaa atcgcgcacg ccgggatctg
     5521 tgcggggggt atccggtaac gggtatccct accgcgacat gttggcttta atttattgtt
     5581 ttaaatgtca aagaggaaaa cggtatgagt ggtggagacg gtcgaggtcc gggtaattca
     5641 ggtctgggac ataatggtgg tcaggccagt gggaatgtga acggtacgtc tggtaaaggt
     5701 ggcccttcat caggtggtgg tacggatcca aacagcgggc cgggctgggg tacgacgcat
     5761 acgccgaatg gagatattca taactataat ccgggggagt ttggtaacgg cgggagtaaa
     5821 cccggtggta atggtggtaa cagcggcaat catagcggta gctctggtgg cggacagtct
     5881 tcggccactg cgatggcctt cggtctgcct gccctggcta ctccgggggc tgaaggactg
     5941 gctttatcag tttccggcga tgcgttgtcg gccgccgttg ctgatgtgct ggctgccctg
     6001 aaggggccgt ttaagtttgg tctgtggggg attgcgatct acggtgtgct gccttctgag
     6061 attgcaaaag atgatccgaa aatgatgtca aaaattatga cgtcattacc ggccgatgcg
     6121 gtgacggaga ctccggcaag tactttacca ctggaccagg cgacggttcg tgtcagacaa
     6181 cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg cggttgtcgc aggtcggcca
     6241 atgagtgtcc ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt cagtgtgtcg
     6301 attccgggtc tcccgtctct gcaggtgagc gtaccgaaag gtgttccgac agcgaaagcc
     6361 ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc cggctggttt tacagccggt
     6421 ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga ccggacagaa gccggtttat
     6481 gtgtcggtga cagatgttct taccccggca caggtaaaac agcgtcagga ggaagaaaag
     6541 cgtcgccagc aggcatggga cgccgctcat ccggaagagg ggctgaaaag agactatgat
     6601 aaagcgaaag ctgagctgga tgccgaagat aaaaatattg cgaccttaaa cagccgcatt
     6661 gcatcgacag agaaggcgct ccccggtgca agggctgctg ttcaggaagc cgataaaaag
     6721 gtaaaagagg cagaggcaaa taaagatgat tttgtgactt ataacccgcc tcatgaatat
     6781 ggctccgggt ggcaggatca ggttcgctat cttgataagg atattcagaa tcagaatgag
     6841 aaattaaaag cggctcaggc atctttaaac gcaatgaatg aatccttatc cagagataag
     6901 gctgcactta ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca
     6961 gaaaataagt taaatgagga aaagaacaag cctcgcaagg ggactaaaga ctacggccat
     7021 gattatcatc cagtacctaa aacagaagac ataaagggac ttggagagtt aaagcgaggt
     7081 gatcctaaaa cacctaaaca aggtggtggt ggtaagcggg cccgatggta tggcgataaa
     7141 aaacgtaaaa tttatgaatg ggattcccag cacggtgagc ttgaagggta tcgtgccagt
     7201 gatggcgaac acctaggcgc atttgatccc aaaacgggta agcaggttaa agggccagat
     7261 ccaaaacgga acattaaaaa atatctttga gaggtaaata tggggcttaa attacgtttg
     7321 acatggtttg ataagaaaac tgaagagttt aaaggggaag agtattctaa agactttggt
     7381 gacgatggtt ctgtcattga aagcttggga atgcctttaa aggataatat taataatggt
     7441 gagtttgatt tgaagaaaga atgggttcct ttactacagc cattttttaa aaatcaaata
     7501 aaacctgatg tggataattg ttatatagct tttgattatc gggacggtaa ctggtaacaa
     7561 aaaattatca aggatgattt gaaatactga cttccggtgt tcagggatga atgccggagt
     7621 ctttaaatac agggaaagta tatgaaaaag gtaaagacta tttttctttt tatattgatt
     7681 gttgctggct ttcttctggc cgcatgtcag gcaaattata tccgggatgt tcagggcgga
     7741 acggtggcac catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca
     7801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa
     7861 ccacctgggg aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt
     7921 aactggcttt cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc
     7981 gcacacttca agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac
     8041 caatggctgc tgccagtggc gttttgcgtg ctttcccggg ttggactcaa gtgaacagtt
     8101 accggataag gcgcagcagt cgggctgaac ggggagttct tgcttacagc ccagcttgga
     8161 gcgaacgacc tacaccgagc cgagatacca gtgtgtgagc tatgagaaag cgccacactt
     8221 cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc agggtcggaa caggagagcg
     8281 caagagggag cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg
     8341 tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt
     8401 ttatgtgaac tctggcagga gggcggagcc tatggaaaaa cgccaccggc gcggccctgc
     8461 cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg
     8521 tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg
     8581 cgcaatttcc ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc
     8641 ctgattaagt ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt
     8701 tttcgctgaa cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg
     8761 caagtcatcc ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct
     8821 ggacgccacc gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc
     8881 cacgccagaa attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac
     8941 ggtgctggac gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg
     9001 ctcgctatgg cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg
     9061 ccttttcctc tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc
     9121 atggggcgga aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc acggacgtga
     9181 ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg
     9241 ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg
     9301 gcagtggcat atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtaccg
     9361 ggagtattac agtctggccg gtttttttct ggttatcggc ctgtttttcg gtctggctga
     9421 actctgggcc ggccgaatgc gttttgtggg gctgagccat aaggcgcggc gggagtatgc
     9481 cgatcgctat aagcgcgata ataaccggac tgtgccattt tcggtgctgg cctcgctgga
     9541 ttcacccggg cggatggtca gggcacggcg acatgtctgg aactacgcgt actggctgac
     9601 cggcgctgcc ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg
     9661 gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca gattattacc tgctgcagcc
     9721 ggctaacctg ttcgggatag cctggtgtgc cggagggctt gtcgccggcg tccttctggc
     9781 gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa
     9841 cgcgaaactg aatcaggaga gccggcgcag cagccagcgg accggagaaa tgacagacgt
     9901 ccgccacctg catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga
     9961 gcaggcccgg aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct
    10021 ggtccctcgt gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg
    10081 caagagcgcg atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt
    10141 ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc
    10201 ggacttcacc ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga
    10261 tctcgaccag tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg
    10321 cgacgtggcg gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca
    10381 gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc
    10441 cctgaaaaag gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt
    10501 cctgcagacg gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta
    10561 cgtcatcggc tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg
    10621 ctgcgcgcaa atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat
    10681 gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg
    10741 cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg
    10801 gcaggatctg cccgctgatt ttgtgaaaac cacggtactg gataacacgc cgatccgctg
    10861 gttctatcgc gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggagat
    10921 ccgggtggac gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcacatcag
    10981 cggcgacagc ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca
    11041 cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag
    11101 cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac
    11161 ggacccgctg gcggagtacc tgcctccggc cgacggcggc ggaccaaaag cggatgatga
    11221 ttttgcgggg ctttactgat gacactggag cagtacaacg tcagccacgc gaagcggcag
    11281 gcgcggaacg cggagaaaac acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc
    11341 acggcggagc tggttgcgcg acggctgggg attgcggcgg tacagccggt ttaccgcttc
    11401 ctggacagtc tggtggcgaa agggctgctg gtccgggcga aatacccggt tgacggccgt
    11461 caggtcagcg tgtgggggct gacgccgcac ggggtggcgt tctcgttcga cgaggacgag
    11521 ccgctgacgg acgttattcc gtttcagccg tcaagggtgt cggcggcgca gctgccgcac
    11581 cggctggccg tgcagtccct gcgcctctcc atggagggcc ggggggccac cggctggcgg
    11641 tacctgcacc gtatggccct gaaagggatg aaagtgccgg acgcgctggc ggagctggac
    11701 ggcagaacgg tggcatttga ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa
    11761 gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat ctggtacatc
    11821 tgccctgacc ggccgacgct tgtccgggta cagcgggcga ttctggcggt ggatgaaatc
    11881 gtgaatccac agaccggcga ggcccgcaaa acggtggagc tggaccggga gaggctgttc
    11941 gcgtgcttta aatttatgac aacggagtga
//
