LOCUS       NZ_OM001477            11970 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Escherichia coli strain CR-HvKP4TC-2 plasmid pCR-HvKP4TC-2_p4,
            complete sequence.
ACCESSION   NZ_OM001477
VERSION     NZ_OM001477.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Transmission of pLVPK-like virulence plasmid in Klebsiella
            pneumoniae mediated by an Incl1 conjugative helper plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2021) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Tat Chee Avenue, Hong Kong,
            Kowloon 999999, Hong Kong
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.8
            Sequencing Technology        :: Illumina; Oxford Nanopore MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_022810585.1-RS_2024_05_30
            Annotation Date              :: 05/30/2024 03:20:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 14
            CDSs (total)                 :: 14
            Genes (coding)               :: 14
            CDSs (with protein)          :: 14
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OM001477.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP4TC-2"
                     /db_xref="taxon:562"
                     /plasmid="pCR-HvKP4TC-2_p4"
                     /geo_loc_name="China"
                     /lat_lon="22.32 N 114.18 E"
                     /collection_date="2021"
                     /identified_by="Y.XM"
     gene            271..642
                     /gene="tolA"
                     /locus_tag="MUW72_RS00005"
     CDS             271..642
                     /gene="tolA"
                     /locus_tag="MUW72_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            639..1004
                     /locus_tag="MUW72_RS00010"
     CDS             639..1004
                     /locus_tag="MUW72_RS00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1179..1457
                     /locus_tag="MUW72_RS00015"
     CDS             1179..1457
                     /locus_tag="MUW72_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1445..1759
                     /locus_tag="MUW72_RS00020"
     CDS             1445..1759
                     /locus_tag="MUW72_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1923..2351
                     /locus_tag="MUW72_RS00025"
     CDS             1923..2351
                     /locus_tag="MUW72_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2377..2556
                     /locus_tag="MUW72_RS00030"
     CDS             2377..2556
                     /locus_tag="MUW72_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2583..3113)
                     /locus_tag="MUW72_RS00035"
     CDS             complement(2583..3113)
                     /locus_tag="MUW72_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3120..3851)
                     /gene="mobC"
                     /locus_tag="MUW72_RS00040"
     CDS             complement(3120..3851)
                     /gene="mobC"
                     /locus_tag="MUW72_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3851..5815)
                     /locus_tag="MUW72_RS00045"
     CDS             complement(3851..5815)
                     /locus_tag="MUW72_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7299..7448)
                     /locus_tag="MUW72_RS00050"
     CDS             complement(7299..7448)
                     /locus_tag="MUW72_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7533..7790)
                     /locus_tag="MUW72_RS00055"
     CDS             complement(7533..7790)
                     /locus_tag="MUW72_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7800..9485)
                     /locus_tag="MUW72_RS00060"
     CDS             complement(7800..9485)
                     /locus_tag="MUW72_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(9590..11077)
                     /gene="ltrA"
                     /locus_tag="MUW72_RS00065"
     CDS             complement(9590..11077)
                     /gene="ltrA"
                     /locus_tag="MUW72_RS00065"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            11722..11967
                     /locus_tag="MUW72_RS00070"
     CDS             11722..11967
                     /locus_tag="MUW72_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             2800..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6618..7196
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3124..3851)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gtatttacca taacgtcgca ttgcccgcac cgctcagacg gtctgagggc gtttccggcg
       61 gctacgggat aaaacaggca acaaaccacc cggaaaaccg ccggaaacgc gcccggtaaa
      121 ttgtaacccg ctgcatgcct atgcacgcaa ctgcatggcg ggccgtttgg tgtaaaaacg
      181 atacgataag ccgtgtttat gtaaatgaac gtggccgtaa agagtgacaa atatacatgt
      241 aacatttttt actgacaggt catcaacccg atgcgtttaa taccctttat cctgctggca
      301 ttgtgcagcc ccttactcct gacggcctgt aagcattcca ccacccagcc gccgaaaatt
      361 tatactctgg ataagtcatc taaaatcagt agctacgctg cggccatcaa gaccgctgtt
      421 gaaatgcaga tgtttgaacc agaggcctat gcgggaaaaa tgtgcaccgt gcggatatcc
      481 atacaacgtg atggcactct gaacagcgcc attgctgaag gaggagatcc gaaactctgt
      541 aaagcagcaa tatcagccgt tacccacgcc aaaattccgc cggcaccgga tgaaaatacc
      601 tggcaaatat tcaaaaacgc ccctctggat ttcagaccat gaaagctttt actctgacag
      661 tgctggcggc catgttcctc actccggtac atgcgatacc actaaaaccc gtaaccacac
      721 tgcagccaac ctacccggta caggccgcag caaaacgcat cgaaggtcag gtgaccgcca
      781 cgtttgatat tctggacgat ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt
      841 tttttcggga aacgctgacg gcaatgaggc actggctata tcccgcaggg cacccggcaa
      901 tgcgacaggt tataaatttt gaattcagtc tgaaaaatga cggcttacag gatccgcgac
      961 cagtcaccac ccaaaagctc aatgatgatg gttgccgcca gtaacaggtc ctttaacccg
     1021 caggctgcaa tagtcctgga tgatgagtga gcctgtagca gccctgatta gtggaagcga
     1081 agctgaaaaa tgggcagcaa aagccgtatt tctggcaaat agcgccgcca cggattaaag
     1141 tagtcatgtg taatacatat gactacaaag gggcttttat gtcatcagca acatccatca
     1201 ggctcgatga agaacttaag gaccggctga aaacgctggc cgatgaccgg caccgctcag
     1261 cgcacgcgct aatgctggag gcaatcaccg aatacatcga ccgggaagag aagcgcagcc
     1321 agtacctgcg ggacggacag gccgcatggc agcactatca ggaaacgggt ctgcacctta
     1381 ctgcggaaga agcggaagcc tggataagta cctggggaac tgagaacgag caggatgctc
     1441 cgccatgcca caggtaacga tttccgcgct ggcacagcgc gatttacaac gccttcagga
     1501 ctttctcaaa accaaaaatc ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc
     1561 tatccagcaa ctgaaaacat tgccagacat tggccgcccg gtgccgtttc tgccgctgga
     1621 atataaggaa ctggtgatcg ggtttggcga cagtggctat gtgatgctct accgccacga
     1681 ccgggaaatg gaccagattg tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc
     1741 gggggcagac agcctctaat aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc
     1801 ggtttgacaa gcgcagcgcg tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc
     1861 agcgatacat ttaccgtgag aaattacaca cctttcacac ccgataaaat caggggtata
     1921 cagtggcgaa aacattgctc aacctttcac aggcagcaca agccgcgggc attaccagaa
     1981 gaacgctata caatcatgta aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca
     2041 accctgtcgt ggatgtgtca gaactgatcc gtgtatacgg aaatgtgaat atccctgaga
     2101 aacaaattcc cggcatttca caccgtgaaa atacacaaaa aaatttccca caggaacagc
     2161 ttctggcaat gcaaaaggaa ctggccgatc tccggcaggc tgtcaccctg atgctggaag
     2221 ataaaacatc ccgggaagaa gaacgccgcc agcacgatga tgagcgccgg aaactccagg
     2281 ccgaagtgga cagattgaca acagaactga cacagaagaa aaaacgcttc tggtccggct
     2341 ggtttagctg agaacttacc gaagggaatg gagaacatga cgaagcagga aaaaactgcc
     2401 ctcaatatgg ctaaattcat taaggatcag tcgctgctgt tgctggaaaa gctgaacgag
     2461 ctggacatgg ataccccggc cgacatgtgc gaacggcttc acgaggatgc ggaaacactc
     2521 tggcaggtga tgcgggataa gctcggggaa gaataacagc accggccatg ccggtgctgg
     2581 cgttattttt tgccgaacgg ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc
     2641 accggcacgt taaaatagcg gctgttatat tcccgcatca gctccgcttt cagcttctcg
     2701 ttcaccgcgc tttccccgtg ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc
     2761 ggtgagcact tcccggcatc aaccacctgc acaccgttct cgatggcgaa ccgctccacc
     2821 tttcgataga ggtaatcaca cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga
     2881 ttttcaccgg tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc
     2941 ccgatggatt tcatcatcgc cagatgctgg gcccggtcga caaaacagaa aacatacgcc
     3001 ccggtctgcc aggcgagcac caccatgtct tccagatgct cgctggttgg cataatgcag
     3061 cgctcgccgt ctttgtcttc cacgtcctca ataaatacca tgtacagcgc cataatccct
     3121 cactccgttg tcataaattt aaagcacgcg aacagcctct cccggtccag ctccaccgtt
     3181 ttgcgggcct cgccggtctg tggattcacg atttcatcca ccgccagaat cgcccgctgt
     3241 acccggacaa gcgtcggccg gtcagggcag atgtaccaga tttcatcgat gccgttcgcc
     3301 cggcggttga acagataccc ggacaccact tcctgatacc gcctgcggct cttgacggtg
     3361 cgctctacct caaatgccac cgttctgccg tccagctccg ccagcgcgtc cggcactttc
     3421 atccctttca gggccatacg gtgcaggtac cgccagccgg tggccccccg gccctccatg
     3481 gagaggcgca gggactgcac ggccagccgg tgcggcagct gcgccgccga cacccttgac
     3541 ggctgaaacg gaataacgtc cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg
     3601 tgcggcgtca gcccccacac gctgacctga cggccgtcaa ccgggtattt cgcccggacc
     3661 agcagccctt tcgccaccag actgtccagg aagcggtaaa ccggctgtac cgccgcaatc
     3721 cccagccgtc gcgcaaccag ctccgccgtg gaacacagct cttcccgcag ccagcgcagg
     3781 gtcagccgtg ttttctccgc gttccgcgcc tgccgcttcg cgtggctgac gttgtactgc
     3841 tccagtgtca tcagtaaagc cccgcaaaat catcatccgc ttttggtccg ccgccgtcgg
     3901 ccggaggcag gtactccgcc agcgggtccg ttttcgccag caccgggaag gttttcagcg
     3961 aaatttcccg gcgctcaacc cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc
     4021 cggtcatcac cgcgaacccg tcaggcaggt gttgcagggt gttcacgtca aacagcggcc
     4081 gcgcctcctt ctggatgaag ctgtcgccgc tgatgtgctc cacgttcccc gcctcccggc
     4141 tggcccggcg gcgctccacg tccacccgga tctccccggt ctggcccgcc gcccactgcg
     4201 cggactcctg gctggccgcg cgatagaacc agcggatcgg cgtgttatcc agtaccgtgg
     4261 ttttcacaaa atcagcgggc agatcctgcc cgcactgacc gaagtcgccc agcgactggt
     4321 gcgcgagcag caggttgcag tcccggctgc gcagcgtccc cagcgcgttc agcacgtact
     4381 tcgacagcag atatttaatc tcatcgagca tgatgctcgc gtgcggccac ctccggaact
     4441 catcccgcgc aatgatgatt tgcgcgcagc gggcaaacag catcttctgc acccggatca
     4501 ccgcctcatc gtccatcgag ccgatgacgt acaggcagcc gccgccgttg attatccccg
     4561 ccacatcgat accgctgtcc gtctgcagga cgctcaggtc ggccacgttc tccagcttgg
     4621 tgatcagccc cttcacgtcc tttttcaggg cctccggcaa cgcgtacgcc gccgccagca
     4681 cctgctgaat gtttgccccc tgcgggaact gctccgcaat cagcttcgcc gctttctgct
     4741 cgctgatgcg gtagtggtcc gccacgtcgc cggtctcact caggttaaac ccggcaacca
     4801 gcagattctt cagggcgtac tggtcgagat cgcggaacag attcagctgc gccggtttcc
     4861 ccggccgcag atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg tgcggcgccc
     4921 acgcgtcccc tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt acgcagcgaa
     4981 tgagcgcgtt ggtggccatc gcgctcttgc cgctcccggg caggccgagg atctgaatat
     5041 tggtcttgcg ccaggtatca cgagggacca gcaccggctg gccctgctca tccttaccca
     5101 gaaacaccgc ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc gcgttaaccg
     5161 gtaccggctc cccgaaatgc aggtggcgga cgtctgtcat ttctccggtc cgctggctgc
     5221 tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcggcc gcaaaatgac
     5281 catcaagcag caggaacgcc agcagccgcg ccagaaggac gccggcgaca agccctccgg
     5341 cacaccaggc tatcccgaac aggttagccg gctgcagcag gtaataatct gcgttcgcat
     5401 agaggtccgg gcgcaggacg gtttctcccc agctctcgat aaacacggcg acaaccagcc
     5461 cccccagaag ggccagaccg gcagcgccgg tcagccagta cgcgtagttc cagacatgtc
     5521 gccgtgccct gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa aatggcacag
     5581 tccggttatt atcgcgctta tagcgatcgg catactcccg ccgcgcctta tggctcagcc
     5641 ccacaaaacg cattcggccg gcccagagtt cagccagacc gaaaaacagg ccgataacca
     5701 gaaaaaaacc ggccagactg taatactccc ggtacagcca gctgacccac gggtaaatct
     5761 gccgggccag ctccgggata tgccactgcc aggcaagcga atccgtatta aacatgcaat
     5821 ctctctcctt cagacgacgg gtttaccggc gtgatgcgca gcccccgctg cacaagccgc
     5881 ggacatgccc caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt tcagctttca
     5941 ccgcggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg aggaacaacg
     6001 agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt tccggtgttc
     6061 cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc aggagcagct
     6121 ttgctgcttc gtcaggtgcg tccagcaccg tggatttttg tgggtggtca ggcgtgagcc
     6181 tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga gccagaaaga
     6241 ggggaaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaaggtctt ttcccctgaa
     6301 gtcagccacg ccccagcggg gatgacttgc atttctccgc aaggagaaaa aagcaacaga
     6361 gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc ctgataattg
     6421 atcgtcaggg aatagaaaga cttaatcagg ggacaatatc ccacatcagc gcgtccggtg
     6481 accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc tacgctccgg
     6541 gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat tccgtggata
     6601 agcaggggac taacatgtga ggcaaaacgg cagggccgcg ccggtggcgt ttttccatag
     6661 gctccgccct cctgccagag ttcacataaa cagacgcttt ttcggtgcat ctgtgggagc
     6721 cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc
     6781 caccgtttcc ggtaggtcgc tccctcttgc gctctcctgt tccgaccctg ccgtttaccg
     6841 gatacctgtc cgccgttctc ccttgcggga agtgtggcgc tttctcatag ctcacacact
     6901 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     6961 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccgggaaagc
     7021 acgcaaaacg ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag
     7081 ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat
     7141 ttggttgctg tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta
     7201 accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg
     7261 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc
     7321 agttcagagg aggacgatgg tgccaccgtt ccgccctgaa catcccggat ataatttgcc
     7381 tgacatgcgg ccagaagaaa gccagcaaca atcaatataa aaagaaaaat agtctttacc
     7441 tttttcatat actttccctg tatttaaaga ctccggcatt catccctgaa caccggaagt
     7501 cagtatttca aatcatcctt gataattttt tgttaccagt taccgtcccg ataatcaaaa
     7561 gctatataac aattatccac atcaggtttt atttgatttt taaaaaatgg ctgtagtaaa
     7621 ggaacccatt ctttcttcaa atcaaactca ccattattaa tattatcctt taaaggcatt
     7681 cccaagcttt caatgacaga accatcgtca ccaaagtctt tagaatactc ttccccttta
     7741 aactcttcag ttttcttatc aaaccatgtc aaacgtaatt taagccccat atttacctct
     7801 caaagatatt ttttaatgtt ccgttttgga tctggccctt taacctgctt acccgttttg
     7861 ggatcaaatg cgcctaggtg ttcgccatca ctggcacgat acccttcaag ctcaccgtgc
     7921 tgggaatccc attcataaat tttacgtttt ttatcgccat accatcgggc ccgcttacca
     7981 ccaccacctt gtttaggtgt tttaggatca cctcgcttta actctccaag tccctttatg
     8041 tcttctgttt taggtactgg atgataatca tggccgtagt ctttagtccc cttgcgaggc
     8101 ttgttctttt cctcatttaa cttattttct gcatccttcg cttttttctc cttttgtttc
     8161 cggctctcca tcgccccggt aagtgcagcc ttatctctgg ataaggattc attcattgcg
     8221 tttaaagatg cctgagccgc ttttaatttc tcattctgat tctgaatatc cttatcaaga
     8281 tagcgaacct gatcctgcca cccggagcca tattcatgag gcgggttata agtcacaaaa
     8341 tcatctttat ttgcctctgc ctcttttacc tttttatcgg cttcctgaac agcagccctt
     8401 gcaccgggga gcgccttctc tgtcgatgca atgcggctgt ttaaggtcgc aatattttta
     8461 tcttcggcat ccagctcagc tttcgcttta tcatagtctc ttttcagccc ctcttccgga
     8521 tgagcggcgt cccatgcctg ctggcgacgc ttttcttcct cctgacgctg ttttacctgt
     8581 gccggggtaa gaacatctgt caccgacaca taaaccggct tctgtccggt ctctttcggg
     8641 aaacgaataa cggcctcacg ggagttacca ccggctgtaa aaccagccgg acgtgaatca
     8701 cctttttcag caacaatgcc ttttggcggg gctttcgctg tcggaacacc tttcggtacg
     8761 ctcacctgca gagacgggag acccggaatc gacacactga atacccccgg acgttttgtc
     8821 ggtttcgcat ccaccacagg gacactcatt ggccgacctg cgacaaccgc aatatgctgc
     8881 cgctcatcct tcaccacatc cacaacccgt tgtctgacac gaaccgtcgc ctggtccagt
     8941 ggtaaagtac ttgccggagt ctccgtcacc gcatcggccg gtaatgacgt cataattttt
     9001 gacatcattt tcggatcatc ttttgcaatc tcagaaggca gcacaccgta gatcgcaatc
     9061 ccccacagac caaacttaaa cggccccttc agggcagcca gcacatcagc aacggcggcc
     9121 gacaacgcat cgccggaaac tgataaagcc agtccttcag cccccggagt agccagggca
     9181 ggcagaccga aggccatcgc agtggccgaa gactgtccgc caccagagct accgctatga
     9241 ttgccgctgt taccaccatt accaccgggt ttactcccgc cgttaccaaa ctcccccgga
     9301 ttatagttat gaatatctcc attcggcgta tgcgtcgtac cccagcccgg cccgctgttt
     9361 ggatccgtac caccacctga tgaagggcca cctttaccag acgtaccgtt cacattccca
     9421 ctggcctgac caccattatg tcccagacct gaattacccg gacctcgacc gtctccacca
     9481 ctcataccgt tttcctcttt gacatttaaa acaataaatt aaagccaaca tgtcgcggta
     9541 gggatacccg ttaccggata ccccccgcac agatcccggc gtgcgcgatt tacgcaccgg
     9601 gctcctgcct cgggtgtctg gcggtgaacc gctccacagg ccatggatga agaacccgaa
     9661 cccttggtag ccatgcggct gccagtttgt ttgctttcgt ccaggtcgta tcatccttct
     9721 ggctcctgcg cctgagcgcc cggcgccaga ggtttgttac gtgtgtcctg aacttctgca
     9781 tggtggggaa gttgcccggt accgagtgat agttcaggta tccctgaacc actctcctga
     9841 gccattttcc ctgttcgggg attgagtaat gccagcgcct tcgcagaccg tctttgatgg
     9901 ctttcagagt tgccgtcatc cgatcccggc gggtctttcg tatcagcatg aacctgccgt
     9961 tgcgatcttt cccgctgatg tgcgtgaacc cgaggaagtt gaacgtttct ggtttgcctt
    10021 ttcccctgat ggcacggttt tcggcagcga agcggccgaa ctccatcaga cgggttttct
    10081 ccgggtgaac cgtgagtccg aactccctca gtctgcgctg catggctata cggaagcgcc
    10141 gggcatcgta tcgtttgtcg aacccgatga cgatgtcatc ggcgtatctg accattacca
    10201 cattgcctgt ggcatagcga cgtcgccact gatgcgccca cagatcgaag acgtagtgga
    10261 ggtatatgtt tgccagcagc ggtgagatga ccgcaccctg tggggtgcct tcctccgttg
    10321 ctcgccattg accctcctcc gacgtcccgg ctgtgagcca cttacgtatg agcctgatta
    10381 ccctccggtc gccgatccga tgctctgtga acctgatcag ccattcgtgg ctcaccctgt
    10441 cgaagaactg actgatgtcg gcatccagta cccagtttac gttagtgcgt accagccctg
    10501 tggccagtgc gtccagtgca tcgtgctggc ttcgcccggg tctgaacccg tatgagaacc
    10561 ccataaagtc gttttcatag actgcgttca ggattttcac cagcgcatac tggacgatct
    10621 tgtcctccag cgaggcgatg ccgagcgggc gttgttttcc atccgctttt gggatgtagt
    10681 gacgcctgcc gggctgcgcc ctgtagctgc cctgatgtag cctccggtgc agatctgtta
    10741 tgttgttctt catgtttccg gcgtagtcca tccacctgat gccatccact ccggcggccg
    10801 ctttcctgct cagggagagg aatgcggctt ccagtgcttc gactgtcagc aggtggaaca
    10861 atgctgtaaa ccgttctttc ttccgctgct tcgcagcttc ccgcacgcgt gacagcctct
    10921 gtgacatgct ttcccggctc tgtgtccggc gcatgtgtgg ctgttccgcg ttccccttgg
    10981 ccccgctcct tcgctccact gactccgctc ctttcgggtt gttcgcctgc ttcgccgcta
    11041 ctatgagcga gtccgacttc tcctctccgt acatcaccgg ctatgactcc tcgtcttccc
    11101 ggtgcgggcc atctccgaca ctggcagatg gtcagagggg agatctcccg gttcccgcgt
    11161 agagatcgta ttgacatgcc agggtctcag accccgccgg gtccatgtgg cactcgcagt
    11221 atcgcaccct atgatgttgc cttccgttaa cagtacaacg tcggcacccg gtaatttaat
    11281 atacatttcg tggctcaatg gctggcctgt caacacccct gtcaacgctt cgccccatac
    11341 ctcgcggtat gcaacgcatg actcggggac cttgtggatt gctggtcctt caatggtcgg
    11401 ggactttcac cccttgatct ctaaccggtc tcccggcgca cactgtacat atacacagta
    11461 taattattac cttactcctc ccgttttcag gtgtcaatat atgagattag ttctcatctt
    11521 caggcacaaa aaagcgcccg gagccacctg taagcagatc gcgtcttttc ccgaaaattt
    11581 ctcatggcaa caaattttat tatttaagac attcatcaca cttacatttc agatggtata
    11641 gacacatctg aaagctaact ggcatgagga gaggacctgc ggttaaactg ttcgggaaac
    11701 ctgagacgcc cggaggccac tatgacgcac ctgaaactga ctcccgctga agaaaagcgc
    11761 ctggcggacg cctttgtcgc tcaggcactg gccatgggcc tgaaagggaa caaaacgctg
    11821 ccaaaggcgg taaaacaaca ggtgcgggaa agggtgcttc gtgccattca ggacgaacgt
    11881 gaagccaggc gggcgcagga aatgcgtcgg gagaaatacg aggctacgga aaccaccttc
    11941 acctggcagc cacagcgccg ccggtaaggg
//
