LOCUS       NZ_OM001473            11970 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Escherichia coli strain CR-HvKP1TC-3 plasmid pCR-HvKP1TC-3_p4,
            complete sequence.
ACCESSION   NZ_OM001473
VERSION     NZ_OM001473.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Transmission of pLVPK-like virulence plasmid in Klebsiella
            pneumoniae mediated by an Incl1 conjugative helper plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2021) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Tat Chee Avenue, Hong Kong,
            Kowloon 999999, Hong Kong
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.8
            Sequencing Technology        :: Illumina; Oxford Nanopore MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_022810545.1-RS_2024_05_30
            Annotation Date              :: 05/30/2024 03:19:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 14
            CDSs (total)                 :: 14
            Genes (coding)               :: 14
            CDSs (with protein)          :: 14
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OM001473.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP1TC-3"
                     /db_xref="taxon:562"
                     /plasmid="pCR-HvKP1TC-3_p4"
                     /geo_loc_name="China"
                     /lat_lon="22.32 N 114.18 E"
                     /collection_date="2021"
                     /identified_by="Y.XM"
     gene            45..1730
                     /locus_tag="MUW71_RS00005"
     CDS             45..1730
                     /locus_tag="MUW71_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1740..1997
                     /locus_tag="MUW71_RS00010"
     CDS             1740..1997
                     /locus_tag="MUW71_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2082..2231
                     /locus_tag="MUW71_RS00015"
     CDS             2082..2231
                     /locus_tag="MUW71_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3715..5679
                     /locus_tag="MUW71_RS00020"
     CDS             3715..5679
                     /locus_tag="MUW71_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5679..6410
                     /gene="mobC"
                     /locus_tag="MUW71_RS00025"
     CDS             5679..6410
                     /gene="mobC"
                     /locus_tag="MUW71_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6417..6947
                     /locus_tag="MUW71_RS00030"
     CDS             6417..6947
                     /locus_tag="MUW71_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6974..7153)
                     /locus_tag="MUW71_RS00035"
     CDS             complement(6974..7153)
                     /locus_tag="MUW71_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7179..7607)
                     /locus_tag="MUW71_RS00040"
     CDS             complement(7179..7607)
                     /locus_tag="MUW71_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7771..8085)
                     /locus_tag="MUW71_RS00045"
     CDS             complement(7771..8085)
                     /locus_tag="MUW71_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8073..8351)
                     /locus_tag="MUW71_RS00050"
     CDS             complement(8073..8351)
                     /locus_tag="MUW71_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8526..8891)
                     /locus_tag="MUW71_RS00055"
     CDS             complement(8526..8891)
                     /locus_tag="MUW71_RS00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8888..9259)
                     /gene="tolA"
                     /locus_tag="MUW71_RS00060"
     CDS             complement(8888..9259)
                     /gene="tolA"
                     /locus_tag="MUW71_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9533..9778)
                     /locus_tag="MUW71_RS00065"
     CDS             complement(9533..9778)
                     /locus_tag="MUW71_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            10423..11910
                     /gene="ltrA"
                     /locus_tag="MUW71_RS00070"
     CDS             10423..11910
                     /gene="ltrA"
                     /locus_tag="MUW71_RS00070"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..6730
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2335..2913)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5680..6407
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gttggcttta atttattgtt ttaaatgtca aagaggaaaa cggtatgagt ggtggagacg
       61 gtcgaggtcc gggtaattca ggtctgggac ataatggtgg tcaggccagt gggaatgtga
      121 acggtacgtc tggtaaaggt ggcccttcat caggtggtgg tacggatcca aacagcgggc
      181 cgggctgggg tacgacgcat acgccgaatg gagatattca taactataat ccgggggagt
      241 ttggtaacgg cgggagtaaa cccggtggta atggtggtaa cagcggcaat catagcggta
      301 gctctggtgg cggacagtct tcggccactg cgatggcctt cggtctgcct gccctggcta
      361 ctccgggggc tgaaggactg gctttatcag tttccggcga tgcgttgtcg gccgccgttg
      421 ctgatgtgct ggctgccctg aaggggccgt ttaagtttgg tctgtggggg attgcgatct
      481 acggtgtgct gccttctgag attgcaaaag atgatccgaa aatgatgtca aaaattatga
      541 cgtcattacc ggccgatgcg gtgacggaga ctccggcaag tactttacca ctggaccagg
      601 cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg
      661 cggttgtcgc aggtcggcca atgagtgtcc ctgtggtgga tgcgaaaccg acaaaacgtc
      721 cgggggtatt cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc gtaccgaaag
      781 gtgttccgac agcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc
      841 cggctggttt tacagccggt ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga
      901 ccggacagaa gccggtttat gtgtcggtga cagatgttct taccccggca caggtaaaac
      961 agcgtcagga ggaagaaaag cgtcgccagc aggcatggga cgccgctcat ccggaagagg
     1021 ggctgaaaag agactatgat aaagcgaaag ctgagctgga tgccgaagat aaaaatattg
     1081 cgaccttaaa cagccgcatt gcatcgacag agaaggcgct ccccggtgca agggctgctg
     1141 ttcaggaagc cgataaaaag gtaaaagagg cagaggcaaa taaagatgat tttgtgactt
     1201 ataacccgcc tcatgaatat ggctccgggt ggcaggatca ggttcgctat cttgataagg
     1261 atattcagaa tcagaatgag aaattaaaag cggctcaggc atctttaaac gcaatgaatg
     1321 aatccttatc cagagataag gctgcactta ccggggcgat ggagagccgg aaacaaaagg
     1381 agaaaaaagc gaaggatgca gaaaataagt taaatgagga aaagaacaag cctcgcaagg
     1441 ggactaaaga ctacggccat gattatcatc cagtacctaa aacagaagac ataaagggac
     1501 ttggagagtt aaagcgaggt gatcctaaaa cacctaaaca aggtggtggt ggtaagcggg
     1561 cccgatggta tggcgataaa aaacgtaaaa tttatgaatg ggattcccag cacggtgagc
     1621 ttgaagggta tcgtgccagt gatggcgaac acctaggcgc atttgatccc aaaacgggta
     1681 agcaggttaa agggccagat ccaaaacgga acattaaaaa atatctttga gaggtaaata
     1741 tggggcttaa attacgtttg acatggtttg ataagaaaac tgaagagttt aaaggggaag
     1801 agtattctaa agactttggt gacgatggtt ctgtcattga aagcttggga atgcctttaa
     1861 aggataatat taataatggt gagtttgatt tgaagaaaga atgggttcct ttactacagc
     1921 cattttttaa aaatcaaata aaacctgatg tggataattg ttatatagct tttgattatc
     1981 gggacggtaa ctggtaacaa aaaattatca aggatgattt gaaatactga cttccggtgt
     2041 tcagggatga atgccggagt ctttaaatac agggaaagta tatgaaaaag gtaaagacta
     2101 tttttctttt tatattgatt gttgctggct ttcttctggc cgcatgtcag gcaaattata
     2161 tccgggatgt tcagggcgga acggtggcac catcgtcctc ctctgaactg acggggatcg
     2221 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctt
     2281 ttgccctgta aacgaaaaaa ccacctgggg aggtggtttg atcgaaggtt aagtcagttg
     2341 gggaactgct taaccgtggt aactggcttt cgcagagcac agcaaccaaa tctgtccttc
     2401 cagtgtagcc ggactttggc gcacacttca agagcaaccg cgtgtttagc taaacaaatc
     2461 ctctgcgaac tcccagttac caatggctgc tgccagtggc gttttgcgtg ctttcccggg
     2521 ttggactcaa gtgaacagtt accggataag gcgcagcagt cgggctgaac ggggagttct
     2581 tgcttacagc ccagcttgga gcgaacgacc tacaccgagc cgagatacca gtgtgtgagc
     2641 tatgagaaag cgccacactt cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc
     2701 agggtcggaa caggagagcg caagagggag cgacctaccg gaaacggtgg ggatctttaa
     2761 gtcctgtcgg gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga
     2821 tgcaccgaaa aagcgtctgt ttatgtgaac tctggcagga gggcggagcc tatggaaaaa
     2881 cgccaccggc gcggccctgc cgttttgcct cacatgttag tcccctgctt atccacggaa
     2941 tctgtgggta actttgtatg tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag
     3001 cgaccgagtg agcgaggagg cgcaatttcc ccggcacctg caagacggtc accggacgcg
     3061 ctgatgtggg atattgtccc ctgattaagt ctttctattc cctgacgatc aattatcagg
     3121 ataggtgaac catgtcggtt tttcgctgaa cattttacgt taacctatct ctgttgcttt
     3181 tttctccttg cggagaaatg caagtcatcc ccgctggggc gtggctgact tcaggggaaa
     3241 agaccttgct tacgctggct ggacgccacc gacgcacggt cttttcccct ctttctggct
     3301 ctgtccggct ggacgccggc cacgccagaa attcacccgg cccccgtcag gctcacgcct
     3361 gaccacccac aaaaatccac ggtgctggac gcacctgacg aagcagcaaa gctgctcctg
     3421 attttgcggg tggtcagccg ctcgctatgg cgaactctga cgggggccgg aacaccggaa
     3481 aagaccgcac tggggtgccg ccttttcctc tcacacgctg gggcgtgctc gttgttcctc
     3541 cggggctgcg ccttttccgc atggggcgga aagaggctgg cctccgcggt gaaagctgaa
     3601 cgcaatcacc acggacgtga ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc
     3661 agcgggggct gcgcatcacg ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt
     3721 aatacggatt cgcttgcctg gcagtggcat atcccggagc tggcccggca gatttacccg
     3781 tgggtcagct ggctgtaccg ggagtattac agtctggccg gtttttttct ggttatcggc
     3841 ctgtttttcg gtctggctga actctgggcc ggccgaatgc gttttgtggg gctgagccat
     3901 aaggcgcggc gggagtatgc cgatcgctat aagcgcgata ataaccggac tgtgccattt
     3961 tcggtgctgg cctcgctgga ttcacccggg cggatggtca gggcacggcg acatgtctgg
     4021 aactacgcgt actggctgac cggcgctgcc ggtctggccc ttctgggggg gctggttgtc
     4081 gccgtgttta tcgagagctg gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca
     4141 gattattacc tgctgcagcc ggctaacctg ttcgggatag cctggtgtgc cggagggctt
     4201 gtcgccggcg tccttctggc gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg
     4261 gccgctgacg aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag cagccagcgg
     4321 accggagaaa tgacagacgt ccgccacctg catttcgggg agccggtacc ggttaacgcg
     4381 ctggccgatt tttccacgga gcaggcccgg aaacagcagg cggtgtttct gggtaaggat
     4441 gagcagggcc agccggtgct ggtccctcgt gatacctggc gcaagaccaa tattcagatc
     4501 ctcggcctgc ccgggagcgg caagagcgcg atggccacca acgcgctcat tcgctgcgta
     4561 cgcgatttcg gggacgctgt ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac
     4621 gtcttccggg cgcactgccc ggacttcacc ctgctggatc tgcggccggg gaaaccggcg
     4681 cagctgaatc tgttccgcga tctcgaccag tacgccctga agaatctgct ggttgccggg
     4741 tttaacctga gtgagaccgg cgacgtggcg gaccactacc gcatcagcga gcagaaagcg
     4801 gcgaagctga ttgcggagca gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg
     4861 gcgtacgcgt tgccggaggc cctgaaaaag gacgtgaagg ggctgatcac caagctggag
     4921 aacgtggccg acctgagcgt cctgcagacg gacagcggta tcgatgtggc ggggataatc
     4981 aacggcggcg gctgcctgta cgtcatcggc tcgatggacg atgaggcggt gatccgggtg
     5041 cagaagatgc tgtttgcccg ctgcgcgcaa atcatcattg cgcgggatga gttccggagg
     5101 tggccgcacg cgagcatcat gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg
     5161 aacgcgctgg ggacgctgcg cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg
     5221 ggcgacttcg gtcagtgcgg gcaggatctg cccgctgatt ttgtgaaaac cacggtactg
     5281 gataacacgc cgatccgctg gttctatcgc gcggccagcc aggagtccgc gcagtgggcg
     5341 gcgggccaga ccggggagat ccgggtggac gtggagcgcc gccgggccag ccgggaggcg
     5401 gggaacgtgg agcacatcag cggcgacagc ttcatccaga aggaggcgcg gccgctgttt
     5461 gacgtgaaca ccctgcaaca cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg
     5521 gcgcggctgg cgttcagcag cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc
     5581 ttcccggtgc tggcgaaaac ggacccgctg gcggagtacc tgcctccggc cgacggcggc
     5641 ggaccaaaag cggatgatga ttttgcgggg ctttactgat gacactggag cagtacaacg
     5701 tcagccacgc gaagcggcag gcgcggaacg cggagaaaac acggctgacc ctgcgctggc
     5761 tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg acggctgggg attgcggcgg
     5821 tacagccggt ttaccgcttc ctggacagtc tggtggcgaa agggctgctg gtccgggcga
     5881 aatacccggt tgacggccgt caggtcagcg tgtgggggct gacgccgcac ggggtggcgt
     5941 tctcgttcga cgaggacgag ccgctgacgg acgttattcc gtttcagccg tcaagggtgt
     6001 cggcggcgca gctgccgcac cggctggccg tgcagtccct gcgcctctcc atggagggcc
     6061 ggggggccac cggctggcgg tacctgcacc gtatggccct gaaagggatg aaagtgccgg
     6121 acgcgctggc ggagctggac ggcagaacgg tggcatttga ggtagagcgc accgtcaaga
     6181 gccgcaggcg gtatcaggaa gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca
     6241 tcgatgaaat ctggtacatc tgccctgacc ggccgacgct tgtccgggta cagcgggcga
     6301 ttctggcggt ggatgaaatc gtgaatccac agaccggcga ggcccgcaaa acggtggagc
     6361 tggaccggga gaggctgttc gcgtgcttta aatttatgac aacggagtga gggattatgg
     6421 cgctgtacat ggtatttatt gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc
     6481 caaccagcga gcatctggaa gacatggtgg tgctcgcctg gcagaccggg gcgtatgttt
     6541 tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg atgtatgacg
     6601 agcggctcaa cagcgacgaa aaagccacgt actttttcac cggtgaaaat ccgcaatggg
     6661 aacgggacaa acccacagga atgaagctgt gtgattacct ctatcgaaag gtggagcggt
     6721 tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg gagcagatta
     6781 acgcgatgat tgaggtggtc agcgagcggc acggggaaag cgcggtgaac gagaagctga
     6841 aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggtg cctgaactgc
     6901 cggaggacaa acccgcccgc cggtggtggc cgttcggcaa aaaataacgc cagcaccggc
     6961 atggccggtg ctgttattct tccccgagct tatcccgcat cacctgccag agtgtttccg
     7021 catcctcgtg aagccgttcg cacatgtcgg ccggggtatc catgtccagc tcgttcagct
     7081 tttccagcaa cagcagcgac tgatccttaa tgaatttagc catattgagg gcagtttttt
     7141 cctgcttcgt catgttctcc attcccttcg gtaagttctc agctaaacca gccggaccag
     7201 aagcgttttt tcttctgtgt cagttctgtt gtcaatctgt ccacttcggc ctggagtttc
     7261 cggcgctcat catcgtgctg gcggcgttct tcttcccggg atgttttatc ttccagcatc
     7321 agggtgacag cctgccggag atcggccagt tccttttgca ttgccagaag ctgttcctgt
     7381 gggaaatttt tttgtgtatt ttcacggtgt gaaatgccgg gaatttgttt ctcagggata
     7441 ttcacatttc cgtatacacg gatcagttct gacacatcca cgacagggtt gtttttcccg
     7501 tcacgtgaca cggtaacctt tccctgtttt acatgattgt atagcgttct tctggtaatg
     7561 cccgcggctt gtgctgcctg tgaaaggttg agcaatgttt tcgccactgt atacccctga
     7621 ttttatcggg tgtgaaaggt gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc
     7681 gtttttccgt tcaggatgtg gttttctgac gcgctgcgct tgtcaaaccg gcgacggcca
     7741 cgcaatgaat tgcgcccccg tcatcggtta ttagaggctg tctgcccccg gatacccgga
     7801 ttctttctga tgcctgacgg tgacgatcac aatctggtcc atttcccggt cgtggcggta
     7861 gagcatcaca tagccactgt cgccaaaccc gatcaccagt tccttatatt ccagcggcag
     7921 aaacggcacc gggcggccaa tgtctggcaa tgttttcagt tgctggatag cccggacgat
     7981 cacctcacct ccttttctgg ctgccagccg atttttggtt ttgagaaagt cctgaaggcg
     8041 ttgtaaatcg cgctgtgcca gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc
     8101 tcgttctcag ttccccaggt acttatccag gcttccgctt cttccgcagt aaggtgcaga
     8161 cccgtttcct gatagtgctg ccatgcggcc tgtccgtccc gcaggtactg gctgcgcttc
     8221 tcttcccggt cgatgtattc ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc
     8281 cggtcatcgg ccagcgtttt cagccggtcc ttaagttctt catcgagcct gatggatgtt
     8341 gctgatgaca taaaagcccc tttgtagtca tatgtattac acatgactac tttaatccgt
     8401 ggcggcgcta tttgccagaa atacggcttt tgctgcccat ttttcagctt cgcttccact
     8461 aatcagggct gctacaggct cactcatcat ccaggactat tgcagcctgc gggttaaagg
     8521 acctgttact ggcggcaacc atcatcattg agcttttggg tggtgactgg tcgcggatcc
     8581 tgtaagccgt catttttcag actgaattca aaatttataa cctgtcgcat tgccgggtgc
     8641 cctgcgggat atagccagtg cctcattgcc gtcagcgttt cccgaaaaaa aaggtgacag
     8701 ccggtaagcg acacgttgtc cactcgtcca tcgtccagaa tatcaaacgt ggcggtcacc
     8761 tgaccttcga tgcgttttgc tgcggcctgt accgggtagg ttggctgcag tgtggttacg
     8821 ggttttagtg gtatcgcatg taccggagtg aggaacatgg ccgccagcac tgtcagagta
     8881 aaagctttca tggtctgaaa tccagagggg cgtttttgaa tatttgccag gtattttcat
     8941 ccggtgccgg cggaattttg gcgtgggtaa cggctgatat tgctgcttta cagagtttcg
     9001 gatctcctcc ttcagcaatg gcgctgttca gagtgccatc acgttgtatg gatatccgca
     9061 cggtgcacat ttttcccgca taggcctctg gttcaaacat ctgcatttca acagcggtct
     9121 tgatggccgc agcgtagcta ctgattttag atgacttatc cagagtataa attttcggcg
     9181 gctgggtggt ggaatgctta caggccgtca ggagtaaggg gctgcacaat gccagcagga
     9241 taaagggtat taaacgcatc gggttgatga cctgtcagta aaaaatgtta catgtatatt
     9301 tgtcactctt tacggccacg ttcatttaca taaacacggc ttatcgtatc gtttttacac
     9361 caaacggccc gccatgcagt tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg
     9421 cgtttccggc ggttttccgg gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg
     9481 ccctcagacc gtctgagcgg tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc
     9541 ggcgctgtgg ctgccaggtg aaggtggttt ccgtagcctc gtatttctcc cgacgcattt
     9601 cctgcgcccg cctggcttca cgttcgtcct gaatggcacg aagcaccctt tcccgcacct
     9661 gttgttttac cgcctttggc agcgttttgt tccctttcag gcccatggcc agtgcctgag
     9721 cgacaaaggc gtccgccagg cgcttttctt cagcgggagt cagtttcagg tgcgtcatag
     9781 tggcctccgg gcgtctcagg tttcccgaac agtttaaccg caggtcctct cctcatgcca
     9841 gttagctttc agatgtgtct ataccatctg aaatgtaagt gtgatgaatg tcttaaataa
     9901 taaaatttgt tgccatgaga aattttcggg aaaagacgcg atctgcttac aggtggctcc
     9961 gggcgctttt ttgtgcctga agatgagaac taatctcata tattgacacc tgaaaacggg
    10021 aggagtaagg taataattat actgtgtata tgtacagtgt gcgccgggag accggttaga
    10081 gatcaagggg tgaaagtccc cgaccattga aggaccagca atccacaagg tccccgagtc
    10141 atgcgttgca taccgcgagg tatggggcga agcgttgaca ggggtgttga caggccagcc
    10201 attgagccac gaaatgtata ttaaattacc gggtgccgac gttgtactgt taacggaagg
    10261 caacatcata gggtgcgata ctgcgagtgc cacatggacc cggcggggtc tgagaccctg
    10321 gcatgtcaat acgatctcta cgcgggaacc gggagatctc ccctctgacc atctgccagt
    10381 gtcggagatg gcccgcaccg ggaagacgag gagtcatagc cggtgatgta cggagaggag
    10441 aagtcggact cgctcatagt agcggcgaag caggcgaaca acccgaaagg agcggagtca
    10501 gtggagcgaa ggagcggggc caaggggaac gcggaacagc cacacatgcg ccggacacag
    10561 agccgggaaa gcatgtcaca gaggctgtca cgcgtgcggg aagctgcgaa gcagcggaag
    10621 aaagaacggt ttacagcatt gttccacctg ctgacagtcg aagcactgga agccgcattc
    10681 ctctccctga gcaggaaagc ggccgccgga gtggatggca tcaggtggat ggactacgcc
    10741 ggaaacatga agaacaacat aacagatctg caccggaggc tacatcaggg cagctacagg
    10801 gcgcagcccg gcaggcgtca ctacatccca aaagcggatg gaaaacaacg cccgctcggc
    10861 atcgcctcgc tggaggacaa gatcgtccag tatgcgctgg tgaaaatcct gaacgcagtc
    10921 tatgaaaacg actttatggg gttctcatac gggttcagac ccgggcgaag ccagcacgat
    10981 gcactggacg cactggccac agggctggta cgcactaacg taaactgggt actggatgcc
    11041 gacatcagtc agttcttcga cagggtgagc cacgaatggc tgatcaggtt cacagagcat
    11101 cggatcggcg accggagggt aatcaggctc atacgtaagt ggctcacagc cgggacgtcg
    11161 gaggagggtc aatggcgagc aacggaggaa ggcaccccac agggtgcggt catctcaccg
    11221 ctgctggcaa acatatacct ccactacgtc ttcgatctgt gggcgcatca gtggcgacgt
    11281 cgctatgcca caggcaatgt ggtaatggtc agatacgccg atgacatcgt catcgggttc
    11341 gacaaacgat acgatgcccg gcgcttccgt atagccatgc agcgcagact gagggagttc
    11401 ggactcacgg ttcacccgga gaaaacccgt ctgatggagt tcggccgctt cgctgccgaa
    11461 aaccgtgcca tcaggggaaa aggcaaacca gaaacgttca acttcctcgg gttcacgcac
    11521 atcagcggga aagatcgcaa cggcaggttc atgctgatac gaaagacccg ccgggatcgg
    11581 atgacggcaa ctctgaaagc catcaaagac ggtctgcgaa ggcgctggca ttactcaatc
    11641 cccgaacagg gaaaatggct caggagagtg gttcagggat acctgaacta tcactcggta
    11701 ccgggcaact tccccaccat gcagaagttc aggacacacg taacaaacct ctggcgccgg
    11761 gcgctcaggc gcaggagcca gaaggatgat acgacctgga cgaaagcaaa caaactggca
    11821 gccgcatggc taccaagggt tcgggttctt catccatggc ctgtggagcg gttcaccgcc
    11881 agacacccga ggcaggagcc cggtgcgtaa atcgcgcacg ccgggatctg tgcggggggt
    11941 atccggtaac gggtatccct accgcgacat
//
