LOCUS       NZ_OL891655             5596 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KP55 plasmid pKPC-5505, complete
            sequence.
ACCESSION   NZ_OL891655
VERSION     NZ_OL891655.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2021) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Yongchuan,
            Chongqing 402106, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: pKPC-5501
            Coverage                          :: 709
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 04:58:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OL891655.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP55"
                     /isolation_source="Patient"
                     /db_xref="taxon:573"
                     /plasmid="pKPC-5505"
                     /geo_loc_name="China"
     gene            complement(join(5537..5596,1..207))
                     /locus_tag="NQI94_RS00005"
     CDS             complement(join(5537..5596,1..207))
                     /locus_tag="NQI94_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(191..415)
                     /locus_tag="NQI94_RS00010"
                     /old_locus_tag="KDGBBGBG_00425"
     CDS             complement(191..415)
                     /locus_tag="NQI94_RS00010"
                     /old_locus_tag="KDGBBGBG_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            578..706
                     /locus_tag="NQI94_RS00015"
                     /old_locus_tag="KDGBBGBG_00426"
     CDS             578..706
                     /locus_tag="NQI94_RS00015"
                     /old_locus_tag="KDGBBGBG_00426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(1082..1294)
                     /locus_tag="NQI94_RS00020"
     CDS             complement(1082..1294)
                     /locus_tag="NQI94_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1301..1810)
                     /locus_tag="NQI94_RS00025"
     CDS             complement(1301..1810)
                     /locus_tag="NQI94_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1845..2279)
                     /locus_tag="NQI94_RS00030"
                     /pseudo=""
     CDS             complement(<1845..2279)
                     /locus_tag="NQI94_RS00030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2488..2832)
                     /locus_tag="NQI94_RS00035"
                     /old_locus_tag="KDGBBGBG_00428"
     CDS             complement(2488..2832)
                     /locus_tag="NQI94_RS00035"
                     /old_locus_tag="KDGBBGBG_00428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2822..2998)
                     /locus_tag="NQI94_RS00040"
                     /old_locus_tag="KDGBBGBG_00429"
     CDS             complement(2822..2998)
                     /locus_tag="NQI94_RS00040"
                     /old_locus_tag="KDGBBGBG_00429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4154..4480
                     /locus_tag="NQI94_RS00045"
                     /old_locus_tag="KDGBBGBG_00431"
     CDS             4154..4480
                     /locus_tag="NQI94_RS00045"
                     /old_locus_tag="KDGBBGBG_00431"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4506..5051
                     /locus_tag="NQI94_RS00050"
                     /old_locus_tag="KDGBBGBG_00432"
     CDS             4506..5051
                     /locus_tag="NQI94_RS00050"
                     /old_locus_tag="KDGBBGBG_00432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            5117..5467
                     /locus_tag="NQI94_RS00055"
                     /old_locus_tag="KDGBBGBG_00433"
     CDS             5117..5467
                     /locus_tag="NQI94_RS00055"
                     /old_locus_tag="KDGBBGBG_00433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          complement(3085..3161)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3350..3747
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(967..2498)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcggatctca cacagaacgc gataatctcc cacccgatag cgccagaact cgcccagctc
       61 acctttgagc ggcttccctg actggcgagg atcggcagca actgcaatgc gtaaactcat
      121 aaaatccaca atccgtcgtg cgttctgttt gtccatcttg cgtaacgatt tgagcgcccg
      181 atcggaatag ttaatcgtcc agaccaagtg acttcctcac gtcttcagac gaatgcactg
      241 cttcatcacc acggcgaacg cgtgcagcag tttctgctga aagataataa tcctccaggt
      301 cttccagatg cgccagtatt gcttcccgcg cataaaacgt ctttgttctg ccggtttgct
      361 tcgccagcga gtccagacgg gactcaatct catcagataa ccggatagca agcatataaa
      421 cctcccgaac accatttgct atacatgtat atcaacagta ccacaatcaa cctaaaagaa
      481 caaaaaataa tcttgttagc ccctcttgat aatccgtaac gcggtcaata tatctagctt
      541 gagggctttc ctcttaacta ttgaaaggat aacgattatg ggtcttaagc cgggtccgaa
      601 acgaattgcc gattcaacag gggaacctga taaacgccag cgtgacaata aaaaaacgcc
      661 gggaaatact gacaaattga aacccagcaa atcttcaaaa aaataagtca gtagttaaaa
      721 gccctccggg gcttttttgt atctgccgtt cacgctgatt aaacataaca cccgttgccc
      781 gcaccgcccc ggacgggttc gccgcgaacg gggtggcagt caggtaaaac cggcaacaaa
      841 ccacccgata aaccgccccg ttcgcgacag ataaatttta cctggctgca tagccatgca
      901 gccaggtgaa tgacatccgg tttcagcgta aatgcccgat gagaaccctg ttttcctgca
      961 tttcagccca gcgacggacc gtcatagctt cgctgccggg aacgcgcttc cgtcagttcc
     1021 ttttcgtgct gccgttgctc gtgcgcccgt tctgcggcca cggccatctc atgacgctgc
     1081 actaccggct caaacgctgc agttgcccgc tcaagcgttc cacctgctga ctcaagcgcg
     1141 tcacgcgctc gctcagcgcc gcgttctccc gttgcataag ccctgacatt ttccgccatt
     1201 ccccgaaggc gctctcccac tcgtccagcc ttttcgagta gtcctgctgt agctgctcta
     1261 atgcgctcag caactgtttt tccagctcgg tcatcggcac ttactccttc accagtctca
     1321 gcgcgttctg cttcgaccgc ccgttcccga ccgtccagct ctgcgtcctg tctacggccg
     1381 tccccttcgg cagcaccaga aaacgtccgt tgctgtcttc caggtacgtc acgccccagg
     1441 tcttcgcgtt cagcttcgac agcgctgcgt tctgccggtc gatttcggcc agatttgacg
     1501 ctatcaagct cccctgatac cacaacaccc cgctcaggct ggcgaacagc agtcctgaca
     1561 ccatcgtgat ggtcagccat gtccgcccga ccatacgcat cacgctcagg cggttcgact
     1621 gcatggccgc gctcataccc tgctcgtggg cgcggatggc ggcgctgatt ctcttcccgc
     1681 tttcgctcag ttccgctctg acagacttct cgtgctcgct gaatgcggct ttcagcattt
     1741 cgccggtact ctgctgctgc gctttcgatt tctgctctaa gtccttcgcc agcgttaaaa
     1801 gactgttcat agatggctcc cttaagtcgg atgtttcgcc ctccgtccgg gtcggcaatg
     1861 ctgatgctgc tctttgtggt gcgcacgacc tcaaaacctg cgctttccag cgcctcagtg
     1921 acgtcctgac gcgttttaag ctcccctgac gaggcgaggg ccagtaagcc ccgcgtaata
     1981 gcctctgcgg cttcctgctt cgttttgggt aacgaagatg gcgtcaccag cgcgcgccgg
     2041 ttctccggtg cgttcgggtc gtgcagcccc agtcgcccgt tcaccacggt ctgccacgca
     2101 tcgatgcgcg gacggtctgc ccggtcgtag tacggctgga gacgcctgcc ggtcagcagt
     2161 tccgtgttcg gtaccagaaa attcagttcc agccgcccct tgtcccggtg ttcaacccac
     2221 agcacgctgt actggtcttt atcgagtccg ggcatcagaa cccgctcgaa gctcgccatc
     2281 agcttttcac gctggccggg cggtaaatcc tgttcagcaa aagacaggac gccggaggtg
     2341 tactttttgg cgtagggcga ggcgtcgata agctcccgga cctcctccgg ctttccctgc
     2401 aggacgatgg ccccgtcgcg ctggcggtct ttccccagca gataatccac cggcccggca
     2461 ccgccgcctc gccctcgcgg atgaaactta acgatcatcg tctgccccct tttccagcac
     2521 ggcatgccgc agccgctcga gttcggcatc aatggccatc agcgcggcca ccacctgaac
     2581 ccggtcagcg cctgaccact ggccgccgtt aattttccgg gcgatctggt tgaggttgtt
     2641 ccccatcccg gcaagctggc gcagcaaaac cggatcgatg gacggcagcc gcaacgaccg
     2701 cgccgggcgc gtatcgagac aggtttcccg catccacacc gccagttgcc ttccattgca
     2761 gcgctccagc agctgctggt actccccatc ggtcacccgg atacctaaaa aacgggtacg
     2821 cttaaccgcc atcctctcct ccgatatccg caaaatgtga ccgggctgaa cgctcaagct
     2881 ctgccatctg ctcccggcgg taatcttcct cctgtgcctg acgcatcaga tttgccatca
     2941 ctgccggatg ccaggatctt ttttttacga cgagcggctt cttcttcggc ttctgcatta
     3001 tccctccttc gcaaacgccg gacggcacgg aaaagttttc cgggtcgctc cggcgttgga
     3061 caagcgccag cgcgtcagtg gtgttagcgg gtttcggggc gcagccctga accagtcaca
     3121 gcgtttacgc tgtgtatact ggcttactat gttggcatga gagcacgatt catgaaagtg
     3181 cctcatgtgg caggtgtgat atgtggctca ggagcgtccg caataccatg cacggacaga
     3241 tgaaatgacg cctcctcgct cactcggtag ctgcgctcct gccgttcggc tgcggcgggc
     3301 gtgaccgact cacggataac gggataaagc aaaggcgctc aaatcagaaa gggccggaga
     3361 aaaggatgcg gcgttgccgt ttttccatag gctccgcctc ccctgacgag catcacagaa
     3421 atggacgctc aagtcagagg aggacgaaac ccgacaggac ttaaagatgc cggtgatttc
     3481 tctccggcgg ctccctcgtg cgctctcctg ttccgaccct gccgtttacc ggatacctgt
     3541 tccgcctttt tccctttcgg gaagcgtggc gctttctcat agctcacgct gctggtatct
     3601 cggctcggtt taggtcgttc gctccaagct gggctgtatg cacgaacccc ccgttcagcc
     3661 cgactgctgc gccctttccg gtaactatca tcttgagtcc aacccggacg gacacgcaaa
     3721 aacgccactg gcagcagcca ttggtaactg gatatatacg agagagatat tgagaggtct
     3781 tgaagtgggt gcctaactgc ggctacactg aaagaacagt tttggtatct gcggtctcgt
     3841 atacccagtt acccggttaa gcagttcccc aactgactta accttcgatc aaaccgcctc
     3901 cccaggcggt tttttcgttt acagtgcaag agattacgcg cagaaaaaaa ggatctcaag
     3961 aagatccttt aaatgatcgt cagttctgtt atccccggag ctccatccat cccacggaaa
     4021 aacacccgcc actgatgccc tcagcgcact gcacatcatg tacaaaccac tccctccctc
     4081 cttttttatt gcgtttctta atgcgcaatg gtagctttgt gcgcaacaaa aaacgcaata
     4141 gcaagggggg gtaatgagag aaagaacaaa tatcatgcta gatataggat taaagcaatc
     4201 cttacaacgg ctttcgaata aaaccaaaat gagtctaagt gatcttatta accagagatt
     4261 atcaggttca ctttcagttg aagaacgcat tggcgaaaag cctgaatgga tgagaaacct
     4321 tgatccctat ctcgccaatc acttttcaaa tattgagtta ccgctccctt caccattaaa
     4381 agaataccaa gaaaaaatgg atgagataga ttcaattatt aatgacatga tggttctaaa
     4441 ggaatatttc agagctaagt tttacgacga aataaaatag gcacgctata cggaggcgga
     4501 gaattatgtt ttttaaacgg tatttaacgg agaaggaaat atacaaggca gcactcccaa
     4561 ttgttaggct tcaagggaga gttaacactt cattacgttt tttatccaaa tactcagatg
     4621 taaacaaaaa tggattaatt gcattgcatc ttaatttatt acattttatc tcatacaaca
     4681 tacacggtgt agagtctctg aaaatcactc aagcaggcat aaacaaagct gtacaacaaa
     4741 gccctcacgc agatgaaata aaaaatattg cgcaggcaat aggacgcaaa ggacctgagt
     4801 acatcgaagg attaattaga aacccatggg ggaagaaaaa tgataatcca ctaatattta
     4861 taatgagcat tgaagaaagt ataaaagaaa cccttaactc aggttatatg ttatatagag
     4921 tagctcccat aactgagggt gaagcagctg ctttcatgga tgttcttaaa caattattat
     4981 tatttgagga taattttcgc ctttttggta gagacttggt ttataaaata gaaaaattaa
     5041 aaatcagata acaaagtatg gtgaacgaaa aatgaaagag aggatagtga aatgtgtaat
     5101 accaagattc tcaactatgt attggttaat tgcgttccta gcatttatat tccccggtcg
     5161 atcttttgct gacggttaca ccgcttacta ccgatgtaat ggtactcagg aagcatctct
     5221 tactgttaaa gataacaaac taagtatggt tgtaggtctt tctaagttta ataggcaact
     5281 ggatacttat agattcaaag aaaagggaat cgaagtagta atgcagagag ctgcttctga
     5341 tgataactct gaggccataa tgtcttttat tatgcctgac gttaacaata tgaccttatt
     5401 agttaaaaat aacggctaca ttatcgttaa gacaacctgc tggttaacta aaaccgacaa
     5461 acagtagaac acgtagttag tcgagtattt accataatac ctgttgtcgg catcaaaaaa
     5521 gcccgcatag cgggcttcag tcgtaaactt cgcggcgatg tccaatcgtg gcggcaagga
     5581 taacaagctc gtcatc
//
