LOCUS       NZ_OL891654            10059 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KP55 plasmid pKPC-5504, complete
            sequence.
ACCESSION   NZ_OL891654
VERSION     NZ_OL891654.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2021) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Yongchuan,
            Chongqing 402106, China
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.8
            Assembly Name                :: pKPC-5501
            Coverage                     :: 709
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 08/08/2023 04:58:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OL891654.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP55"
                     /isolation_source="Patient"
                     /db_xref="taxon:573"
                     /plasmid="pKPC-5504"
                     /geo_loc_name="China"
     gene            join(9430..10059,1..102)
                     /gene="mobC"
                     /locus_tag="NQI95_RS00005"
     CDS             join(9430..10059,1..102)
                     /gene="mobC"
                     /locus_tag="NQI95_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            109..639
                     /locus_tag="NQI95_RS00010"
                     /old_locus_tag="KDGBBGBG_00411"
     CDS             109..639
                     /locus_tag="NQI95_RS00010"
                     /old_locus_tag="KDGBBGBG_00411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(666..845)
                     /locus_tag="NQI95_RS00015"
                     /old_locus_tag="KDGBBGBG_00412"
     CDS             complement(666..845)
                     /locus_tag="NQI95_RS00015"
                     /old_locus_tag="KDGBBGBG_00412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(871..1299)
                     /locus_tag="NQI95_RS00020"
                     /old_locus_tag="KDGBBGBG_00413"
     CDS             complement(871..1299)
                     /locus_tag="NQI95_RS00020"
                     /old_locus_tag="KDGBBGBG_00413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(1463..1777)
                     /locus_tag="NQI95_RS00025"
                     /old_locus_tag="KDGBBGBG_00414"
     CDS             complement(1463..1777)
                     /locus_tag="NQI95_RS00025"
                     /old_locus_tag="KDGBBGBG_00414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1765..2043)
                     /locus_tag="NQI95_RS00030"
                     /old_locus_tag="KDGBBGBG_00415"
     CDS             complement(1765..2043)
                     /locus_tag="NQI95_RS00030"
                     /old_locus_tag="KDGBBGBG_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(2218..2583)
                     /locus_tag="NQI95_RS00035"
                     /old_locus_tag="KDGBBGBG_00416"
     CDS             complement(2218..2583)
                     /locus_tag="NQI95_RS00035"
                     /old_locus_tag="KDGBBGBG_00416"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(2580..2951)
                     /gene="tolA"
                     /locus_tag="NQI95_RS00040"
                     /old_locus_tag="KDGBBGBG_00417"
     CDS             complement(2580..2951)
                     /gene="tolA"
                     /locus_tag="NQI95_RS00040"
                     /old_locus_tag="KDGBBGBG_00417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(3225..3470)
                     /locus_tag="NQI95_RS00045"
                     /old_locus_tag="KDGBBGBG_00418"
     CDS             complement(3225..3470)
                     /locus_tag="NQI95_RS00045"
                     /old_locus_tag="KDGBBGBG_00418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3797..5482
                     /locus_tag="NQI95_RS00050"
                     /old_locus_tag="KDGBBGBG_00419"
     CDS             3797..5482
                     /locus_tag="NQI95_RS00050"
                     /old_locus_tag="KDGBBGBG_00419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5492..5749
                     /locus_tag="NQI95_RS00055"
                     /old_locus_tag="KDGBBGBG_00420"
     CDS             5492..5749
                     /locus_tag="NQI95_RS00055"
                     /old_locus_tag="KDGBBGBG_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5834..5983
                     /locus_tag="NQI95_RS00060"
                     /old_locus_tag="KDGBBGBG_00421"
     CDS             5834..5983
                     /locus_tag="NQI95_RS00060"
                     /old_locus_tag="KDGBBGBG_00421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7466..9430
                     /locus_tag="NQI95_RS00065"
                     /old_locus_tag="KDGBBGBG_00424"
     CDS             7466..9430
                     /locus_tag="NQI95_RS00065"
                     /old_locus_tag="KDGBBGBG_00424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..10059
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6087..6664)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.11"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9431..10059
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="100.0"
                     /evalue="3.58e-117"
ORIGIN
        1 gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca aaacggtgga gctggaccgg
       61 gagaggctgt tcgcgtgctt taaatttatg acaacggagt gagggattat ggcgctgtac
      121 atggtattta ttgaggacgt ggaagacaaa gacggcgagc gctgcattat gccaaccagc
      181 gagcatctgg aagacatggt ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt
      241 gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga cgagcggctc
      301 aacagcgacg aaaaagccac gtactttttc accggtgaaa atccgcaatg ggaacgggac
      361 aaacccacag gaatgaagct gtgtgattac ctctatcgaa aggtggagcg gttcgccatc
      421 gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat taacgcgatg
      481 attgaggtgg tcagcgagcg gcacggggaa agcgcggtga acgagaagct gaaagcggag
      541 ctgatgcggg aatataacag ccgctatttt aacgtgccgg tgcctgaact gccggaggac
      601 aaacccgccc gccggtggtg gccgttcggc aaaaaataac gccagcaccg gcatggccgg
      661 tgctgttatt cttccccgag cttatcccgc atcacctgcc agagtgtttc cgcatcctcg
      721 tgaagccgtt cgcacatgtc ggccggggta tccatgtcca gctcgttcag cttttccagc
      781 aacagcagcg actgatcctt aatgaattta gccatattga gggcagtttt ttcctgcttc
      841 gtcatgttct ccattccctt cggtaagttc tcagctaaac cagccggacc agaagcgttt
      901 tttcttctgt gtcagttctg ttgtcaatct gtccacttcg gcctggagtt tccggcgctc
      961 atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta tcttccagca tcagggtgac
     1021 agcctgccgg agatcggcca gttccttttg cattgccaga agctgttcct gtgggaaatt
     1081 tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt ttctcaggga tattcacatt
     1141 tccgtataca cggatcagtt ctgacacatc cacgacaggg ttgtttttcc cgtcacgtga
     1201 cacggtaacc tttccctgtt ttacatgatt gtatagcgtt cttctggtaa tgcccgcggc
     1261 ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact gtatacccct gattttatcg
     1321 ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc tgaattttac ccgtttttcc
     1381 gttcaggatg tggttttctg acgcgctgcg cttgtcaaac cggcgacggc cacgcaatga
     1441 attgcgcccc cgtcatcggt tattagaggc tgtctgcccc cggatacccg gattctttct
     1501 gatgcctgac ggtgacgatc acaatctggt ccatttcccg gtcgtggcgg tagagcatca
     1561 catagccact gtcgccaaac ccgatcacca gttccttata ttccagcggc agaaacggca
     1621 ccgggcggcc aatgtctggc aatgttttca gttgctggat agcccggacg atcacctcac
     1681 ctccttttct ggctgccagc cgatttttgg ttttgagaaa gtcctgaagg cgttgtaaat
     1741 cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc ggagcatcct gctcgttctc
     1801 agttccccag gtacttatcc aggcttccgc ttcttccgca gtaaggtgca gacccgtttc
     1861 ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac tggctgcgct tctcttcccg
     1921 gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc gctgagcggt gccggtcatc
     1981 ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc ctgatggatg ttgctgatga
     2041 cataaaagcc cctttgtagt catatgtatt acacatgact actttaatcc gtggcggcgc
     2101 tatttgccag aaatacggct tttgctgccc atttttcagc ttcgcttcca ctaatcaggg
     2161 ctgctacagg ctcactcatc atccaggact attgcagcct gcgggttaaa ggacctgtta
     2221 ctggcggcaa ccatcatcat tgagcttttg ggtggtgact ggtcgcggat cctgtaagcc
     2281 gtcatttttc agactgaatt caaaatttat aacctgtcgc attgccgggt gccctgcggg
     2341 atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa aaaaggtgac agccggtaag
     2401 cgacacgttg tccactcgtc catcgtccag aatatcaaac gtggcggtca cctgaccttc
     2461 gatgcgtttt gctgcggcct gtaccgggta ggttggctgc agtgtggtta cgggttttag
     2521 tggtatcgca tgtaccggag tgaggaacat ggccgccagc actgtcagag taaaagcttt
     2581 catggtctga aatccagagg ggcgtttttg aatatttgcc aggtattttc atccggtgcc
     2641 ggcggaattt tggcgtgggt aacggctgat attgctgctt tacagagttt cggatctcct
     2701 ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta tggatatccg cacggtgcac
     2761 atttttcccg cataggcctc tggttcaaac atctgcattt caacagcggt cttgatggcc
     2821 gcagcgtagc tactgatttt agatgactta tccagagtat aaattttcgg cggctgggtg
     2881 gtggaatgct tacaggccgt caggagtaag gggctgcaca atgccagcag gataaagggt
     2941 attaaacgca tcgggttgat gacctgtcag taaaaaatgt tacatgtata tttgtcactc
     3001 tttacggcca cgttcattta cataaacacg gcttatcgta tcgtttttac accaaacggc
     3061 ccgccatgca gttgcgtgca taggcatgca gcgggttaca atttaccggg cgcgtttccg
     3121 gcggttttcc gggtggtttg ttgcctgttt tatcccgtag ccgccggaaa cgccctcaga
     3181 ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata ccccttaccg gcggcgctgt
     3241 ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct cccgacgcat ttcctgcgcc
     3301 cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc tttcccgcac ctgttgtttt
     3361 accgcctttg gcagcgtttt gttccctttc aggcccatgg ccagtgcctg agcgacaaag
     3421 gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca ggtgcgtcat agtggcctcc
     3481 gggcgtctca ggtttcccga acagtttaac cgcaggtcct ctcctcatgc cagttagctt
     3541 tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa tgtcttaaat aataaaattt
     3601 gttgccatga gaaattttcg ggaaaagacg cgatctgctt acaggtggct ccgggcgctt
     3661 ttttgtgcct gaagatgaga actaatctca tatattgaca cctgaaaacg ggaggagtaa
     3721 ggtaataatt atactgtgta tatgtacagt atgttggctt taatttattg ttttaaatgt
     3781 caaagaggaa aacggtatga gtggtggaga cggtcgaggt ccgggtaatt caggtctggg
     3841 acataatggt ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag gtggcccttc
     3901 atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc atacgccgaa
     3961 tggagatatt cataactata atccggggga gtttggtaac ggcgggagta aacccggtgg
     4021 taatggtggt aacagcggca atcatagcgg tagctctggt ggcggacagt cttcggccac
     4081 tgcgatggcc ttcggtctgc ctgccctggc tactccgggg gctgaaggac tggctttatc
     4141 agtttccggc gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc tgaaggggcc
     4201 gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg agattgcaaa
     4261 agatgatccg aaaatgatgt caaaaattat gacgtcatta ccggccgatg cggtgacgga
     4321 gactccggca agtactttac cactggacca ggcgacggtt cgtgtcagac aacgggttgt
     4381 ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc caatgagtgt
     4441 ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt cgattccggg
     4501 tctcccgtct ctgcaggtga gcgtaccgaa aggtgttccg acagcgaaag ccccgccaaa
     4561 aggcattgtt gctgaaaaag gtgattcacg tccggctggt tttacagccg gtggtaactc
     4621 ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag aagccggttt atgtgtcggt
     4681 gacagatgtt cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa agcgtcgcca
     4741 gcaggcatgg gacgccgctc atccggaaga ggggctgaaa agagactatg ataaagcgaa
     4801 agctgagctg gatgccgaag ataaaaatat tgcgacctta aacagccgca ttgcatcgac
     4861 agagaaggcg ctccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga
     4921 ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg
     4981 gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg agaaattaaa
     5041 agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata aggctgcact
     5101 taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa
     5161 gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc atgattatca
     5221 tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag gtgatcctaa
     5281 aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata aaaaacgtaa
     5341 aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca gtgatggcga
     5401 acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag atccaaaacg
     5461 gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt tgacatggtt
     5521 tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg gtgacgatgg
     5581 ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg gtgagtttga
     5641 tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa taaaacctga
     5701 tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac aaaaaattat
     5761 caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga gtctttaaat
     5821 acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga ttgttgctgg
     5881 ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg gaacggtggc
     5941 accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct
     6001 tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg
     6061 ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccggg taactggctt
     6121 tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc cggactttgg cgcacacttc
     6181 aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg
     6241 ctgccagtgg cgttttgcgt gctttcccgg gttggactca agtgaacagt taccggataa
     6301 ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg agcgaacgac
     6361 ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact tcccgcaagg
     6421 gagaacggcg gacaggtatc cggtaaacgg cagggtcgga acaggagagc gcaagaggga
     6481 gcgacctacc ggaaacggtg gggatcttta agtcctgtcg ggtttcgccc gtactgtcag
     6541 attcatggtt gagcctcacg gctcccacag atgcaccgaa aaagcgtctg tttatgtgaa
     6601 ctctggcagg agggcggagc ctatggaaaa acgccaccgg cgcggccctg ccgttttgcc
     6661 tcacatgtta gtcccctgct tatccacgga atctgtgggt aactttgtat gtgtccgcag
     6721 cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt gagcgaggag gcgcaatttc
     6781 cccggcacct gcaagacggt caccggacgc gctgatgtgg gatattgtcc cctgattaag
     6841 tctttctatt ccctgacgat caattatcag gataggtgaa ccatgtcggt ttttcgctga
     6901 acattttacg ttaacctatc tctgttgctt ttttctcctt gcggagaaat gcaagtcatc
     6961 cccgctgggg cgtggctgac ttcaggggaa aagaccttgc ttacgctggc tggacgccac
     7021 cgacgcacgg tcttttcccc tctttctggc tctgtccggc tggacgccgg ccacgccaga
     7081 aattcacccg gcccccgtca ggctcacgcc tgaccaccca caaaaatcca cggtgctgga
     7141 cgcacctgac gaagcagcaa agctgctcct gattttgcgg gtggtcagcc gctcgctatg
     7201 gcgaactctg acgggggccg gaacaccgga aaagaccgca ctggggtgcc gccttttcct
     7261 ctcacacgct ggggcgtgct cgttgttcct ccggggctgc gccttttccg catggggcgg
     7321 aaagaggctg gcctccgcgg tgaaagctga acgcaatcac cacggacgtg accgccgccg
     7381 ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc tgcgcatcac gccggtaaac
     7441 ccgtcgtctg aaggagagag attgcatgtt taatacggat tcgcttgcct ggcagtggca
     7501 tatcccggag ctggcccggc agatttaccc gtgggtcagc tggctgtacc gggagtatta
     7561 cagtctggcc ggtttttttc tggttatcgg cctgtttttc ggtctggctg aactctgggc
     7621 cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg cgggagtatg ccgatcgcta
     7681 taagcgcgat aataaccgga ctgtgccatt ttcggtgctg gcctcgctgg attcacccgg
     7741 gcggatggtc agggcacggc gacatgtctg gaactacgcg tactggctga ccggcgctgc
     7801 cggtctggcc cttctggggg ggctggttgt cgccgtgttt atcgagagct ggggagaaac
     7861 cgtcctgcgc ccggacctct atgcgaacgc agattattac ctgctgcagc cggctaacct
     7921 gttcgggata gcctggtgtg ccggagggct tgtcgccggc gtccttctgg cgcggctgct
     7981 ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac gaggcggtga acgcgaaact
     8041 gaatcaggag agccggcgca gcagccagcg gaccggagaa atgacagacg tccgccacct
     8101 gcatttcggg gagccggtac cggttaacgc gctggccgat ttttccacgg agcaggcccg
     8161 gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc tggtccctcg
     8221 tgatacctgg cgcaagacca atattcagat cctcggcctg cccgggagcg gcaagagcgc
     8281 gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg tggtgtactt
     8341 cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc cggacttcac
     8401 cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg atctcgacca
     8461 gtacgccctg aagaatctgc tggttgccgg gtttaacctg agtgagaccg gcgacgtggc
     8521 ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc agttcccgca
     8581 gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ttgccggagg ccctgaaaaa
     8641 ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg tcctgcagac
     8701 ggacagcggt atcgatgtgg cggggataat caacggcggc ggctgcctgt acgtcatcgg
     8761 ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc gctgcgcgca
     8821 aatcatcatt gcgcgggatg agttccggag gtggccgcac gcgagcatca tgctcgatga
     8881 gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc gcagccggga
     8941 ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg ggcaggatct
     9001 gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct ggttctatcg
     9061 cgcggccagc caggagtccg cgcagtgggc ggcgggccag accggggaga tccgggtgga
     9121 cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcacatca gcggcgacag
     9181 cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac acctgcctga
     9241 cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca gcccgctgcg
     9301 ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa cggacccgct
     9361 ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg attttgcggg
     9421 gctttactga tgacactgga gcagtacaac gtcagccacg cgaagcggca ggcgcggaac
     9481 gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc cacggcggag
     9541 ctggttgcgc gacggctggg gattgcggcg gtacagccgg tttaccgctt cctggacagt
     9601 ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc
     9661 gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga gccgctgacg
     9721 gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca ccggctggcc
     9781 gtgcagtccc tgcgcctctc catggagggc cggggggcca ccggctggcg gtacctgcac
     9841 cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg cggagctgga cggcagaacg
     9901 gtggcatttg aggtagagcg caccgtcaag agccgcaggc ggtatcagga agtggtgtcc
     9961 gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat ctgccctgac
    10021 cggccgacgc ttgtccgggt acagcgggcg attctggcg
//
