LOCUS       NZ_MW940623            12806 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Escherichia sp. strain SH12R plasmid pSH12R-tetX4, complete
            sequence.
ACCESSION   NZ_MW940623
VERSION     NZ_MW940623.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia sp
  ORGANISM  Escherichia sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Li,R. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2021) Yangzhou University, Veterinary College,
            Shuangqiao Street, Yangzhou, Jiangsu 225000, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: Unicycler
            Sequencing Technology             :: nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/08/2024 02:20:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 15
            CDSs (total)                      :: 15
            Genes (coding)                    :: 12
            CDSs (with protein)               :: 12
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW940623.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Escherichia sp."
                     /mol_type="genomic DNA"
                     /strain="SH12R"
                     /isolation_source="pork"
                     /db_xref="taxon:1884818"
                     /plasmid="pSH12R-tetX4"
                     /geo_loc_name="China"
                     /lat_lon="32.11 N 92.13 E"
                     /collection_date="2019"
                     /collected_by="Yangzhou university"
     gene            1..840
                     /locus_tag="KSF37_RS00005"
                     /old_locus_tag="PDMDJMMM_00001"
     CDS             1..840
                     /locus_tag="KSF37_RS00005"
                     /old_locus_tag="PDMDJMMM_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_094337857.1"
                     /translation="MATHKPINILEAFTAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVLYLPAEDPPTAIHHRLHALGAYLNDEQRQAVAA
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="KSF37_RS00010"
                     /old_locus_tag="PDMDJMMM_00002"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="KSF37_RS00010"
                     /old_locus_tag="PDMDJMMM_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1973..>2389
                     /locus_tag="KSF37_RS00015"
                     /old_locus_tag="PDMDJMMM_00003"
                     /pseudo=""
     CDS             1973..>2389
                     /locus_tag="KSF37_RS00015"
                     /old_locus_tag="PDMDJMMM_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            2747..3769
                     /locus_tag="KSF37_RS00020"
                     /old_locus_tag="PDMDJMMM_00004"
     CDS             2747..3769
                     /locus_tag="KSF37_RS00020"
                     /old_locus_tag="PDMDJMMM_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_000083351.1"
                     /translation="MSQSHAHDHGHDHDHDHTPTVTSANERKVLVSFFLIFGFMLVEAV
                     GGVLSGSLALLADAGHMLTDAVALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLNAV
                     TLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLINVLVLWIMTRGETDHVNVKGA
                     ILHVMGDLLGSVGAIVAAIVIYFTGWTPIDPILSVLVAALILRSAWKLLAKSIHILLEG
                     APEDASPEKVEQGLMSTVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKRVE
                     VVLREQFSIEHATVGIDWNSEADENICSLQPTTDRQDHSGHGHSEHNHDHDHSAPGHKH
                     "
                     /name="cation diffusion facilitator family transporter"
     gene            3813..4190
                     /locus_tag="KSF37_RS00025"
                     /old_locus_tag="PDMDJMMM_00006"
     CDS             3813..4190
                     /locus_tag="KSF37_RS00025"
                     /old_locus_tag="PDMDJMMM_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000203935.1"
                     /translation="MTSRKTTSSAIAHCSTSSHGATTDVLALSQSDVAVLAETFRLLGD
                     QSRLKILLQCMRGSVAVGDIADSLDLSQSLVSHHLRLLRGARLVRGERQAKHIFYGIAD
                     QHVSQVLQDMAVHISEDRNDD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(<4295..4456)
                     /locus_tag="KSF37_RS00030"
                     /old_locus_tag="PDMDJMMM_00007"
                     /pseudo=""
     CDS             complement(<4295..4456)
                     /locus_tag="KSF37_RS00030"
                     /old_locus_tag="PDMDJMMM_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4795..5952)
                     /locus_tag="KSF37_RS00035"
                     /old_locus_tag="PDMDJMMM_00008"
     CDS             complement(4795..5952)
                     /locus_tag="KSF37_RS00035"
                     /old_locus_tag="PDMDJMMM_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline-inactivating monooxygenase Tet(X4)"
                     /protein_id="WP_094309310.1"
                     /translation="MSNKEKQMNLLSDKNVAIIGGGPVGLTMAKLLQQNGIDVSVYERD
                     NDREARIFGGTLDLHKGSGQEAMKKAGLLQTYYDLALPMGVNIADEKGNILSTKNVKPE
                     NRFDNPEINRNDLRAILLNSLENDTVIWDRKLVMLEPGKKKWTLTFENKPSETADLVII
                     ANGGMSKVRKFVTDTEVEETGTFNIQADIHHPEVNCPGFFQLCNGNRLMAAHQGNLLFA
                     NPNNNGALHFGISFKTPDEWKNQTQVDFQNRNSVVDFLLKEFSDWDERYKELIRVTSSF
                     VGLATRIFPLGKSWKSKRPLPITMIGDAAHLMPPFAGQGVNSGLMDALILSDNLTNGKF
                     NSIEEAIENYEQQMFIYGKEAQEESTQNEIEMFKPDFTFQQLLNV"
                     /name="tetracycline-inactivating monooxygenase Tet(X4)"
     gene            complement(5942..6787)
                     /gene="estT"
                     /locus_tag="KSF37_RS00040"
                     /old_locus_tag="PDMDJMMM_00009"
     CDS             complement(5942..6787)
                     /gene="estT"
                     /locus_tag="KSF37_RS00040"
                     /old_locus_tag="PDMDJMMM_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014053561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide hydrolase EstT"
                     /protein_id="WP_164538741.1"
                     /translation="MKEKIIKTNGIELCTESFGNKKNPAILLVAGATVSMLYWDTEFCQ
                     QLSEKGFFVIRYDNRDVGKSTNYEPGSAPYDIVDLTNDAIAILDGYEIDKAHFVGMSLG
                     GLITQIAAIKFADRVSSLTLMSTGPWGDSDPTIPEMDTRILDFHGKAGTVDWSNEDSVV
                     NYLIQGAELMCGKKQFDKQRSEKLIRAEFNRANNYISMFNHATLQGSEEYWNRLNEVNQ
                     PTLIIHGTDDKIWHYKNAGVLIEKIQNTKLITLEGTGHELHFEDWTTIIDGIEKHINEQ
                     "
                     /name="estT"
     gene            complement(7572..9065)
                     /locus_tag="KSF37_RS00045"
                     /old_locus_tag="PDMDJMMM_00010"
     CDS             complement(7572..9065)
                     /locus_tag="KSF37_RS00045"
                     /old_locus_tag="PDMDJMMM_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_001120888.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            complement(9709..9876)
                     /locus_tag="KSF37_RS00050"
                     /old_locus_tag="PDMDJMMM_00011"
     CDS             complement(9709..9876)
                     /locus_tag="KSF37_RS00050"
                     /old_locus_tag="PDMDJMMM_00011"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161403145.1"
                     /translation="MTDLKLDDYTDADFIDELLSDELRCECGEELHYYDDGSLSRCNSC
                     DPDRETNPNL"
                     /name="hypothetical protein"
     gene            complement(9903..10187)
                     /locus_tag="KSF37_RS00055"
                     /old_locus_tag="PDMDJMMM_00012"
     CDS             complement(9903..10187)
                     /locus_tag="KSF37_RS00055"
                     /old_locus_tag="PDMDJMMM_00012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_094336938.1"
                     /translation="MAKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPA"
                     /name="mobilization protein"
     gene            10387..>11028
                     /locus_tag="KSF37_RS00070"
                     /pseudo=""
     CDS             10387..>11028
                     /locus_tag="KSF37_RS00070"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            11135..11548
                     /locus_tag="KSF37_RS00075"
     CDS             11135..11548
                     /locus_tag="KSF37_RS00075"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_223384840.1"
                     /translation="MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRETQAAWAKLAKELREVASEVSDAAQSARSAARGWHWKLWLTVMAASM
                     MPTLVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            11647..12516
                     /locus_tag="KSF37_RS00080"
     CDS             11647..12516
                     /locus_tag="KSF37_RS00080"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229025478.1"
                     /translation="MNREWSASEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SELDLEDMKAEGREPALIVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRTGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRYGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELAQAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            12572..12778
                     /gene="urfF"
                     /locus_tag="KSF37_RS00065"
                     /old_locus_tag="PDMDJMMM_00014"
     CDS             12572..12778
                     /gene="urfF"
                     /locus_tag="KSF37_RS00065"
                     /old_locus_tag="PDMDJMMM_00014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_094336940.1"
                     /translation="MKDHRDTQTGNLLATPNAIRQARFADAQRKLGRKARKIWATDDEA
                     EALRLYLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             complement(4799..5952)
                     /name="tet(X4)"
                     /gene="tet(X4)"
                     /gene_name="tetracycline-inactivating monooxygenase
                     Tet(X4)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1974..2488
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="63.1"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.813"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          10388..12513
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.769"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttca cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgctctacc tgcccgccga agatccaccc
      241 accgccatcc atcaccggct tcacgccctg ggcgcatacc tcaacgacga gcagcggcaa
      301 gccgtggccg ctggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct tatgctattc
     1861 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     1921 ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa
     1981 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     2041 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     2101 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     2161 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     2221 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     2281 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     2341 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg gtcatgggct
     2401 taccatcgac cttcattgtc atgtctctga tcggcttggc cggcgtggtg ctggcgctct
     2461 ggctgtggcc ggccagcgac ccttctgacg tggtgcatga ccaccccagc ttgccgccgg
     2521 atcaccctca tttacgcaca cacgcagacc gaggcaagca ccatcaccag ctgattgtgg
     2581 atgacctgca ccgtatctgg ccgaaaggat aggcacattt tctcgttgcg gcaccacaac
     2641 gtatggcttc ggcggctggg aagggtgagc gaaaatactt cacatgaaca tgtattcatg
     2701 tgttatattt tcaccatagt caaacactca accagcagag gcgcccatgt cccaatccca
     2761 tgcacatgac catggtcacg accatgatca cgaccacacc cccacagtga ccagcgccaa
     2821 cgagcgcaag gttctggttt ccttcttcct gatcttcggc ttcatgctcg tggaagctgt
     2881 cggcggcgtg ctgtcgggct cgctagccct acttgccgat gctggccaca tgctgaccga
     2941 cgccgtggca cttgccctgg cttacgctgc cttccgattc ggtcgccgcg cagccgacag
     3001 caaacgcaca ttcggttacc tgcgcttcga ggtcatcgcc ggcttcctga acgccgtgac
     3061 cctgttcgcg atcgtcgcat ggatcgccta cgaggcgtgg gagcggctgc aggcgccgcc
     3121 cgtcatcctc gccggcccga tgatgatcgt cgccgtgctc ggcttactga tcaacgtcct
     3181 cgtgctgtgg atcatgaccc gcggcgaaac cgatcacgtc aatgtgaaag gtgccattct
     3241 ccacgtgatg ggtgacctgc tgggctctgt cggcgccatc gttgcagcga tcgtcatcta
     3301 tttcaccggc tggacgccga tcgaccccat tctgtcggtc ttggtggcag cgttgattct
     3361 gcgcagcgcc tggaagctcc tggccaagtc gatccatatc ctgctggaag gcgcgccaga
     3421 ggatgcttcg ccggaaaagg tggaacaagg cctgatgagc actgtacccg gcctggcagc
     3481 tgtcagccac gttcatgtgt ggcaactcac ttccggtcgc acgatggcca cactgcacgt
     3541 tcgacccaat gtggacgagg aggcgcgagc cgtggtcaag cgcgtcgagg tggtgcttcg
     3601 agagcaattc agcatcgaac acgccacagt cggaatcgac tggaactcgg aggctgacga
     3661 gaacatctgc agcctgcaac ccacgacaga ccgtcaggac cacagcggcc atggccacag
     3721 tgagcacaat catgaccatg accactccgc tcctggtcac aagcattgag gccgtgcgag
     3781 ccggctcgca gttcacttga gggtttgaat ccatgacatc acgcaaaacc accagctccg
     3841 ctattgctca ctgttcgacc tccagccacg gcgcgaccac tgatgtcctc gcactgtcgc
     3901 aaagtgacgt cgctgttctg gccgagacct tccgcctgct gggcgaccaa tcgcgattga
     3961 aaatcctgtt gcagtgcatg cgcggatccg tcgcagtggg cgacattgcc gattcgctag
     4021 acctgtcgca atccttggtc agccatcacc tgcgcctgtt gcgcggcgcg cgcctcgtgc
     4081 gcggcgagcg ccaggccaag cacatcttct acggcatcgc cgaccagcac gtcagccagg
     4141 tactgcagga catggcggtt cacatttcgg aggacagaaa cgacgattga ctgaacgtgt
     4201 gagtgcgcgc cggcctactc cttcaagggg taggcttttt tttggctatc aatctagaga
     4261 aacaccgacg cgccttgctg aatgcctaga accccacata gcccggcaat tcctttccct
     4321 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     4381 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     4441 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     4501 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     4561 gtgcccgtca ctcccactca atagttggca caaagtccta ttctaggcac taccattact
     4621 ttacaacgcc tgaagaaaag aggatatgtt tccttaactg aattacattt acaacttaac
     4681 ccatctcttt gcgaaccgcc gagtacgtga cccgtacgct cggtggtgtg agaggcgcac
     4741 tccgtcagtt actggcggag ccgtctactc gattatgtgc cgttttattc cattttatac
     4801 atttaacaat tgctgaaacg taaagtcggg tttaaacatt tcaatttcgt tttgagttga
     4861 ttcttcttgt gcttctttgc catagataaa catttgctgt tcataatttt caatagcctc
     4921 ttcaatgctg ttaaatttcc cattggtcag attatccgac aatatcaagg catccatcaa
     4981 cccgctgttt acgccttgtc ctgcaaaagg aggcatcaaa tgagcagcat ctccaatcat
     5041 cgttatgggt aatggacgct tacttttcca agacttacct aagggaaata ttcgtgtcgc
     5101 taaccctaca aaagatgatg tcacacgaat cagttctttg tagcgttcgt cccaatcgga
     5161 aaattctttc agaagaaaat caacgacact atttctgttt tgaaaatcta cctgcgtttg
     5221 gtttttccat tcatcaggtg ttttaaaact tattccaaaa tgcaatgcac cattattatt
     5281 aggattcgca aataataaat taccttgatg agcagccatt agccggtttc cattgcatag
     5341 ctgaaaaaat ccaggacagt tcacctctgg atgatgaata tcggcttgta tattgaaagt
     5401 acctgtttct tcaacttccg tgtcggtaac aaattttctt actttagaca ttccaccatt
     5461 ggcaataata accagatctg ctgtttcact cggtttattc tcaaaagtta gtgtccactt
     5521 cttcttacca ggttcaagca taacaagttt tctatcccaa ataaccgtgt cgttttctaa
     5581 actattcaac aagatagccc ttaagtcatt tctgtttatt tcaggattgt caaatcgatt
     5641 ttcgggcttt acattttttg tggataaaat attgcctttt tcatcagcaa tatttacacc
     5701 cattggtaag gctaagtcat aataagtttg taacaatccc gcttttttca ttgcttcctg
     5761 acctgaacct ttgtgtaggt caagggttcc accaaaaatt cttgcctctc ggtcgttgtc
     5821 tctttcgtaa actgaaacgt ctatgccgtt ttgctgtaat aattttgcca tagtcagtcc
     5881 aacgggtcca ccaccaatta ttgcaacgtt cttatcacta agtaaattca tttgtttttc
     5941 tttattgctc atttatatgt ttttcaattc cgtcaattat tgttgtccaa tcttcaaagt
     6001 gcaattcgtg tcctgttcct tcaagagtaa ttaatttcgt attttgtatt ttctcaatta
     6061 atacaccagc gtttttatag tgccaaattt tgtcatctgt tccgtgaatt atcaaggttg
     6121 gttgatttac ttcatttagt ctattccaat attcttcact accttgcaat gtagcgtgat
     6181 tgaacatact tatataattg tttgctctat tgaactcagc tcttatcagc ttttcacttc
     6241 tttgtttatc aaattgtttc ttgccgcaca ttaattctgc accctgaatt aaatagttta
     6301 ccacactgtc ttcatttgac caatcgactg tgcctgcttt accatggaaa tctaaaatac
     6361 gtgtgtccat ctcaggaata gttgggtccg agtctcccca agggcctgta gacataagtg
     6421 ttaatgagct aactctatcg gcaaacttta tagctgctat ttgtgtaatt agcccaccta
     6481 atgacatacc cacaaaatgt gctttgtcta tttcatagcc gtccaaaatt gcaatggcat
     6541 catttgttaa gtcaacaatg tcgtatggtg ctgaacccgg ctcataatta gtggattttc
     6601 ctacatctct gttgtcgtaa cgaataacaa aaaatccttt ttcagataat tgttggcaaa
     6661 attcagtgtc ccaatacagc attgatacgg ttgcacctgc taccaaaagg attgctggat
     6721 ttttcttatt tccaaaactt tccgtacaga gttcaatgcc gtttgtttta attattttct
     6781 ctttcatttc tgttttcctt tgtttgcaac tgattattat cagtcctaaa attaatatcc
     6841 aaagtagttt tttttttcat ttatcaatgt ttaactgtta tgctggtatc tttcacaatg
     6901 cccgctaact tatatatata cgaaacattc tactctattt catacccata tggcaagata
     6961 ggcgaatgtt tttactattt tatttcgcat tttgaatttc tcaaatataa aaattatttt
     7021 aacattttag tacttatagt gcttaattat ttatgtttca aatcttatga aatcatataa
     7081 tatataaaaa tatatgtata tataaatata ttaaagttgt gtcaaatgtt aaaatttaaa
     7141 attaacagaa aaaggagttt atagttagta aaaaatttat aacttgggtt ttgaaaaaga
     7201 aaatccatga aaaagaaatt aatttatatg tcgatagcga cctttgctat cgcacagagt
     7261 tctattgctc agaaccttga tttacagaca ccagcaaaca atcttaagca acaaatttca
     7321 tcaatatttc ctattgacgt ataggaagaa taaacgccct tttcacccaa gtccaacagc
     7381 tttggaccgc agttgactct ttcgacaccc ctgcgatgca acccaatccg gctgacgggg
     7441 agccagcaac gctgaaaatt taccctcctc tttcccacta gcggctcctt ttccgacaac
     7501 cagcacggcg gatccctgcc gcggcgctgt gaacgcagca ttttgattgg tatcgttggc
     7561 cttcaggctc gtcagtcaaa cagacccagg agcagctcag ccggtggcgc ccggctttcg
     7621 ggtaacgccc tggtcccgct ggtttcggct ttgtgcttca ggtgatcaag gatctgcttg
     7681 atcactatag ggtcttcaat gcaggcgatg actttcatgg cgccgccgca gccgctgcag
     7741 gtctcgatgt cgatattgaa aacacgcttg agccgttgcg cccatgtcat cgacgctcgc
     7801 cgttgtgctg gtgttgccgg ttcatcagcc accctgacct tgttgcccct gccccgtttt
     7861 gccggcgtga ccaacgcccg gtgccgactg ttgggtgcga acaccccgtg gaagcgggtt
     7921 aggttgactc tgggcttcgg taccagggcg gccagccttg caatgaaatc caatggttcg
     7981 aaaatgacgt gcgtggtgcc gtcccggtac ggcgtcttga gctggtagcg cacgttgccg
     8041 cctcgtgtta acgacagccg cttctcggat accgccgggc ggctgatgta ccggcacagc
     8101 cgttcgagct tcttgcgttc atcggccctg gccgccacgc cggcgtgcag gctggacccg
     8161 gctaccttgc caatcccgtc accgaacgga tcaccactgg tcggcagagt ttgcaaagtg
     8221 aacacctttc gccccgcctg tgaaccgaca gcgatacggt aagtgatcga gtgccccagc
     8281 aggggtgtca tcgggtcgtc atccaccgca tccgaggcca gatagctgtt ttcgacatcc
     8341 cgttccagca ggccttgccg ttccagatag cgacccaccc ggtgggcgat ggtgtgcgtc
     8401 agctgggtga gctctgggct ggtcggcgcc ttgacccagc ggaaacgcgc tgagccgtgg
     8461 gattgctcga catacacacc gtcgagaaac agcatgtgga agtgaacatt cagattgagc
     8521 gccgatccaa aacgctggat cagggtgacc gcgcccgtct tggccacttg gtgggtatgg
     8581 cccgctttct tgaccaggtg cgtggcaatg acgcggtaaa cgatgcccag cacccacccc
     8641 atgatctcgg gccggctggc aaacaggaaa cgcagctgaa acgggaagct caacacccac
     8701 tgacgcatgg gttgttcagg cagtacttca tcaaccagca aggcggcact ttcggccatc
     8761 cgccgcgccc cacagctcgg gcagaaaccg cgacgcttac agctgaaagc gaccaggtgc
     8821 tcggcgtggc aagactcgca gcgaacccgt agaaagccat gctccagccg cccgcattgg
     8881 agaaattctt caaattcccg ttgcacatag cccggcaatt cctttccctg ctctgccata
     8941 agcgcagcga atgccgggta atactcgtca acgatctgat agagaagggt ttgctcgggt
     9001 cggtggctct ggtaacgacc agtatcccga tcccggctgg ccgtcctggc cgccacatga
     9061 ggcatgttcc gcgtccttgc aatactgtgt ttacatacag tctatcgctt agcggaaagt
     9121 tcttttaccc tcagccgaaa tgcctgccgt tgctagacat tgccagccag tgcccgtcac
     9181 tcccgtacta actgtcacga acccctgcaa taactgtcac gcccccctgc aataactgtc
     9241 acgaacccct gcaataactg tcacgccccc ctgcaataac tgtcacgaac ccctgcaata
     9301 actgtcacgc ccccaaacct gcaaacccag caggggcggg ggctggcggg gtgttggaaa
     9361 aatccatcca tgattatcta agaataatcc actaggcgcg gttatcagcg cccttgtggg
     9421 gcgctgctgc ccttgcccaa tatgcccggc cagaggccgg atagctggtc tattcgctgc
     9481 gctaggctac acaccgcccc accgctgcgc ggcaggggga aaggcgggca aagcccgcta
     9541 aaccccacac caaaccccgc agaaatacgc tggagcgctt ttagccgctt tagcggcctt
     9601 tccccctacc cgaagggtgg gggcgcgtgt gcagccccgc agggcctgtc tcggtcgatc
     9661 attcagcatc ggcatcatct gctaatttcc cgcaatgttc gcaggtaatc ataggtttgg
     9721 attggtttct ctatctggat cacacgaatt gcagcgactc aatgaaccat catcgtaata
     9781 atgaagctcc tctccgcatt cacagcgcag ttcatcactg agcagttcat caataaaatc
     9841 agcgtctgtg taatcgtcta gttttaaatc agtcattatt gctttccttt attagtttaa
     9901 attcaggccg gctcatcctt ctggcgtggc ggcagaccga acaaggcccg gtcgtggtcg
     9961 cgttcaaggt acgcatccat tgccgccatg agccgatctt ctggccactc gctgctgttc
    10021 accttggcca gaatcatggc gcccaccagc accttgcgcc gcgtttcgtt cttgcgctct
    10081 tgctgctgct cccttgccct cacccgctga atctcggcat tgatccgtgc tcgctgttct
    10141 tcgagcttgg ccagcctatc cgccgccttg ttgctcccct tagccatctt gacacctcat
    10201 tgttaatgtg ctgtctctta ggctatcatg gaggcacgac ggcggcaaac cccgaccgta
    10261 ctttgtacgg gagggcgcac ttaccagttt ctcttcgaga aactggccta acggccaccc
    10321 ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa gcaacagcga
    10381 ggcagcatgg cgatttatca ccttacggcg aaaaccggca gcaggtcggg cggccaatcg
    10441 gccaaggcca aggccgacta catccagcgc gaaggcaagt atgcccgcga catggatgaa
    10501 gtgctgcacg ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc cgactactgg
    10561 gatgctgccg acctgcatga acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc
    10621 ctgccggtcg agctgaccct cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac
    10681 ctgaccggtg ccgaacgttt gccgtatacg ctggccatcc atgccggtgg cggcgagaac
    10741 ccgcactgcc acctgatgat ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct
    10801 cagtggttca agcggtacaa cggcaaggcc ccggagaagg gcggagcgca gaagaccgaa
    10861 gcactcaagc ccaaggcatg gcttgagcag acccgcgagg catgggccga ccatgccaac
    10921 cgggcattag agcgggctgg ccacgacgcc cgcattgacc accgaacgct tgaggcgcag
    10981 ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg tggtggagat ggaaggccgg
    11041 ggcattcgca ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa cgcccagatc
    11101 atcgacttgc aggaataccg ggaggctata gaccatgagc gcaatcgaca gagtgaagaa
    11161 atccagagga atcaacgagt tagcggagca gatcgagccg ctggcccaga gcatggcgac
    11221 actggccgac gaggcccggc aggtcatgag ccagacccag caggccagcg aggcgcaggc
    11281 agcggagtgg ctgaaagccc agcgcgagac acaggcagca tgggccaagc tggccaagga
    11341 attgcgggaa gtggccagcg aggtgagcga cgccgcgcag agcgcccgga gcgcggccag
    11401 agggtggcac tggaagctgt ggctaaccgt gatggcggct tccatgatgc ctacgctggt
    11461 gctgctgatc gcatcgttgc tcttgctcga cctgacgcca ctgacaacag aggacggctc
    11521 gatctggctg cgcttggtgg cccgatgaaa aacgacagaa ccttgcaggc cataggccga
    11581 cagctcaagg ccatgggctg tgagcgcttc gatatcggcg tcagggatgc caccaccggc
    11641 cagatgatga accgggaatg gtcagcctcc gaagtgctcc agaacacgcc atggctcaag
    11701 cggatgaatg cccagggcaa tgacgtgtat atcaggcccg ccgagcagga acgccatggt
    11761 ctggtgctgg tggacgacct cagcgagctc gacctggagg acatgaaagc cgagggccgg
    11821 gaaccggcgc tgatcgtgga gaccagcccg aagaactatc aggcatgggt caaggtggcc
    11881 gacgccgcag gcggtgaact ccgggggcag attgcccgga cgctggccag cgagtacgac
    11941 gccgacccgg ccagcgccga cagccgccac tatggccgct tggcgggttt caccaaccgc
    12001 aaggacaagc acaccacccg caccggctat cagccgtggg tgctgctgcg tgagtccaag
    12061 ggcaagaccg ccaccgctgg cccggcgctg gtgcagcagg ctggccagca gatcgagcag
    12121 gcccagcggc agcaggagaa ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag
    12181 cttagccgcc accggcgcac ggcgctggac gagtaccgca gcgagatggc cggactggtc
    12241 aagcgctacg gtgacgacct cagcaagtgc gactttatcg ccgcgcagaa actggccagc
    12301 cggggccgca gtgccgagga aatcggcaag gccatggccg aggccagccc ggcgctggca
    12361 gagcgcaagc ccggccacga agcggattac atcgagcgca ccgtcagcaa ggttatgggt
    12421 ctgcccagcg tccagcttgc gcgggccgag ctggcacagg caccggcacc ccgccagcga
    12481 ggcatggaca ggggcgggcc agatttcagc atgtaacgct tgcattagcg ctaacgggta
    12541 gtatactgtt agctctaatg gaggatttga gatgaaagac catcgggaca cgcagaccgg
    12601 caacctgttg gccacgccca acgcgatcag gcaggctagg tttgccgacg ctcagcgaaa
    12661 actgggccgc aaggcccgta agatatgggc cacagacgac gaggccgagg cgctgcgtct
    12721 gtacctggaa gaactcagag cggcgcaggg cgggggtagt gaccccgcca gcgcctaacc
    12781 accaactgcc tgaacaggag gcaatc
//
