LOCUS       NZ_MW940617            12805 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Escherichia sp. strain HS2-1 plasmid pHS2-1-tetX4, complete
            sequence.
ACCESSION   NZ_MW940617
VERSION     NZ_MW940617.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia sp
  ORGANISM  Escherichia sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Li,R. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2021) Yangzhou University, Veterinary College,
            Shuangqiao Street, Yangzhou, Jiangsu 225000, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: Unicycler
            Sequencing Technology             :: nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/08/2024 02:18:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 15
            CDSs (total)                      :: 15
            Genes (coding)                    :: 12
            CDSs (with protein)               :: 12
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW940617.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12805
                     /organism="Escherichia sp."
                     /mol_type="genomic DNA"
                     /strain="HS2-1"
                     /isolation_source="pork"
                     /db_xref="taxon:1884818"
                     /plasmid="pHS2-1-tetX4"
                     /geo_loc_name="China"
                     /lat_lon="32.11 N 92.13 E"
                     /collection_date="2019"
                     /collected_by="Yangzhou university"
     gene            1..840
                     /locus_tag="KSF36_RS00005"
                     /old_locus_tag="NHKKCAHH_00001"
     CDS             1..840
                     /locus_tag="KSF36_RS00005"
                     /old_locus_tag="NHKKCAHH_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_094337857.1"
                     /translation="MATHKPINILEAFTAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVLYLPAEDPPTAIHHRLHALGAYLNDEQRQAVAA
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="KSF36_RS00010"
                     /old_locus_tag="NHKKCAHH_00002"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="KSF36_RS00010"
                     /old_locus_tag="NHKKCAHH_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1973..>2389
                     /locus_tag="KSF36_RS00015"
                     /old_locus_tag="NHKKCAHH_00003"
                     /pseudo=""
     CDS             1973..>2389
                     /locus_tag="KSF36_RS00015"
                     /old_locus_tag="NHKKCAHH_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            2747..3769
                     /locus_tag="KSF36_RS00020"
                     /old_locus_tag="NHKKCAHH_00004"
     CDS             2747..3769
                     /locus_tag="KSF36_RS00020"
                     /old_locus_tag="NHKKCAHH_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_000083351.1"
                     /translation="MSQSHAHDHGHDHDHDHTPTVTSANERKVLVSFFLIFGFMLVEAV
                     GGVLSGSLALLADAGHMLTDAVALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLNAV
                     TLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLINVLVLWIMTRGETDHVNVKGA
                     ILHVMGDLLGSVGAIVAAIVIYFTGWTPIDPILSVLVAALILRSAWKLLAKSIHILLEG
                     APEDASPEKVEQGLMSTVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKRVE
                     VVLREQFSIEHATVGIDWNSEADENICSLQPTTDRQDHSGHGHSEHNHDHDHSAPGHKH
                     "
                     /name="cation diffusion facilitator family transporter"
     gene            3813..4190
                     /locus_tag="KSF36_RS00025"
                     /old_locus_tag="NHKKCAHH_00006"
     CDS             3813..4190
                     /locus_tag="KSF36_RS00025"
                     /old_locus_tag="NHKKCAHH_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000203935.1"
                     /translation="MTSRKTTSSAIAHCSTSSHGATTDVLALSQSDVAVLAETFRLLGD
                     QSRLKILLQCMRGSVAVGDIADSLDLSQSLVSHHLRLLRGARLVRGERQAKHIFYGIAD
                     QHVSQVLQDMAVHISEDRNDD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(<4295..4456)
                     /locus_tag="KSF36_RS00030"
                     /old_locus_tag="NHKKCAHH_00007"
                     /pseudo=""
     CDS             complement(<4295..4456)
                     /locus_tag="KSF36_RS00030"
                     /old_locus_tag="NHKKCAHH_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4795..5952)
                     /locus_tag="KSF36_RS00035"
                     /old_locus_tag="NHKKCAHH_00008"
     CDS             complement(4795..5952)
                     /locus_tag="KSF36_RS00035"
                     /old_locus_tag="NHKKCAHH_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline-inactivating monooxygenase Tet(X4)"
                     /protein_id="WP_094309310.1"
                     /translation="MSNKEKQMNLLSDKNVAIIGGGPVGLTMAKLLQQNGIDVSVYERD
                     NDREARIFGGTLDLHKGSGQEAMKKAGLLQTYYDLALPMGVNIADEKGNILSTKNVKPE
                     NRFDNPEINRNDLRAILLNSLENDTVIWDRKLVMLEPGKKKWTLTFENKPSETADLVII
                     ANGGMSKVRKFVTDTEVEETGTFNIQADIHHPEVNCPGFFQLCNGNRLMAAHQGNLLFA
                     NPNNNGALHFGISFKTPDEWKNQTQVDFQNRNSVVDFLLKEFSDWDERYKELIRVTSSF
                     VGLATRIFPLGKSWKSKRPLPITMIGDAAHLMPPFAGQGVNSGLMDALILSDNLTNGKF
                     NSIEEAIENYEQQMFIYGKEAQEESTQNEIEMFKPDFTFQQLLNV"
                     /name="tetracycline-inactivating monooxygenase Tet(X4)"
     gene            complement(5942..6859)
                     /gene="estT"
                     /locus_tag="KSF36_RS00040"
                     /old_locus_tag="NHKKCAHH_00009"
     CDS             complement(5942..6859)
                     /gene="estT"
                     /locus_tag="KSF36_RS00040"
                     /old_locus_tag="NHKKCAHH_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide hydrolase EstT"
                     /protein_id="WP_094309312.1"
                     /translation="MKKKLLWILILGLIIISCKQRKTEMKEKIIKTNGIELCTESFGNK
                     KNPAILLVAGATVSMLYWDTEFCQQLSEKGFFVIRYDNRDVGKSTNYEPGSAPYDIVDL
                     TNDAIAILDGYEIDKAHFVGMSLGGLITQIAAIKFADRVSSLTLMSTGPWGDSDPTIPE
                     MDTRILDFHGKAGTVDWSNEDSVVNYLIQGAELMCGKKQFDKQRSEKLIRAEFNRANNY
                     ISMFNHATLQGSEEYWNRLNEVNQPTLIIHGTDDKIWHYKNAGVLIEKIQNTKLITLEG
                     TGHELHFEDWTTIIDGIEKHINEQ"
                     /name="estT"
     gene            complement(7571..9064)
                     /locus_tag="KSF36_RS00045"
                     /old_locus_tag="NHKKCAHH_00010"
     CDS             complement(7571..9064)
                     /locus_tag="KSF36_RS00045"
                     /old_locus_tag="NHKKCAHH_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_001120888.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            complement(9708..9875)
                     /locus_tag="KSF36_RS00050"
                     /old_locus_tag="NHKKCAHH_00011"
     CDS             complement(9708..9875)
                     /locus_tag="KSF36_RS00050"
                     /old_locus_tag="NHKKCAHH_00011"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161403145.1"
                     /translation="MTDLKLDDYTDADFIDELLSDELRCECGEELHYYDDGSLSRCNSC
                     DPDRETNPNL"
                     /name="hypothetical protein"
     gene            complement(9902..10186)
                     /locus_tag="KSF36_RS00055"
                     /old_locus_tag="NHKKCAHH_00012"
     CDS             complement(9902..10186)
                     /locus_tag="KSF36_RS00055"
                     /old_locus_tag="NHKKCAHH_00012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_094336938.1"
                     /translation="MAKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPA"
                     /name="mobilization protein"
     gene            10386..>11027
                     /locus_tag="KSF36_RS00070"
                     /pseudo=""
     CDS             10386..>11027
                     /locus_tag="KSF36_RS00070"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            11134..11547
                     /locus_tag="KSF36_RS00075"
     CDS             11134..11547
                     /locus_tag="KSF36_RS00075"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_223384840.1"
                     /translation="MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRETQAAWAKLAKELREVASEVSDAAQSARSAARGWHWKLWLTVMAASM
                     MPTLVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            11646..12515
                     /locus_tag="KSF36_RS00080"
     CDS             11646..12515
                     /locus_tag="KSF36_RS00080"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229025478.1"
                     /translation="MNREWSASEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SELDLEDMKAEGREPALIVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRTGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRYGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELAQAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            12571..12777
                     /gene="urfF"
                     /locus_tag="KSF36_RS00065"
                     /old_locus_tag="NHKKCAHH_00014"
     CDS             12571..12777
                     /gene="urfF"
                     /locus_tag="KSF36_RS00065"
                     /old_locus_tag="NHKKCAHH_00014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_094336940.1"
                     /translation="MKDHRDTQTGNLLATPNAIRQARFADAQRKLGRKARKIWATDDEA
                     EALRLYLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             complement(4799..5952)
                     /name="tet(X4)"
                     /gene="tet(X4)"
                     /gene_name="tetracycline-inactivating monooxygenase
                     Tet(X4)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1974..2488
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="63.1"
     Typing          10141..10297
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.671"
                     /coverage="100.0"
                     /evalue="1.08e-59"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.813"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          10387..12512
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.769"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttca cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgctctacc tgcccgccga agatccaccc
      241 accgccatcc atcaccggct tcacgccctg ggcgcatacc tcaacgacga gcagcggcaa
      301 gccgtggccg ctggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct tatgctattc
     1861 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     1921 ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa
     1981 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     2041 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     2101 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     2161 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     2221 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     2281 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     2341 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg gtcatgggct
     2401 taccatcgac cttcattgtc atgtctctga tcggcttggc cggcgtggtg ctggcgctct
     2461 ggctgtggcc ggccagcgac ccttctgacg tggtgcatga ccaccccagc ttgccgccgg
     2521 atcaccctca tttacgcaca cacgcagacc gaggcaagca ccatcaccag ctgattgtgg
     2581 atgacctgca ccgtatctgg ccgaaaggat aggcacattt tctcgttgcg gcaccacaac
     2641 gtatggcttc ggcggctggg aagggtgagc gaaaatactt cacatgaaca tgtattcatg
     2701 tgttatattt tcaccatagt caaacactca accagcagag gcgcccatgt cccaatccca
     2761 tgcacatgac catggtcacg accatgatca cgaccacacc cccacagtga ccagcgccaa
     2821 cgagcgcaag gttctggttt ccttcttcct gatcttcggc ttcatgctcg tggaagctgt
     2881 cggcggcgtg ctgtcgggct cgctagccct acttgccgat gctggccaca tgctgaccga
     2941 cgccgtggca cttgccctgg cttacgctgc cttccgattc ggtcgccgcg cagccgacag
     3001 caaacgcaca ttcggttacc tgcgcttcga ggtcatcgcc ggcttcctga acgccgtgac
     3061 cctgttcgcg atcgtcgcat ggatcgccta cgaggcgtgg gagcggctgc aggcgccgcc
     3121 cgtcatcctc gccggcccga tgatgatcgt cgccgtgctc ggcttactga tcaacgtcct
     3181 cgtgctgtgg atcatgaccc gcggcgaaac cgatcacgtc aatgtgaaag gtgccattct
     3241 ccacgtgatg ggtgacctgc tgggctctgt cggcgccatc gttgcagcga tcgtcatcta
     3301 tttcaccggc tggacgccga tcgaccccat tctgtcggtc ttggtggcag cgttgattct
     3361 gcgcagcgcc tggaagctcc tggccaagtc gatccatatc ctgctggaag gcgcgccaga
     3421 ggatgcttcg ccggaaaagg tggaacaagg cctgatgagc actgtacccg gcctggcagc
     3481 tgtcagccac gttcatgtgt ggcaactcac ttccggtcgc acgatggcca cactgcacgt
     3541 tcgacccaat gtggacgagg aggcgcgagc cgtggtcaag cgcgtcgagg tggtgcttcg
     3601 agagcaattc agcatcgaac acgccacagt cggaatcgac tggaactcgg aggctgacga
     3661 gaacatctgc agcctgcaac ccacgacaga ccgtcaggac cacagcggcc atggccacag
     3721 tgagcacaat catgaccatg accactccgc tcctggtcac aagcattgag gccgtgcgag
     3781 ccggctcgca gttcacttga gggtttgaat ccatgacatc acgcaaaacc accagctccg
     3841 ctattgctca ctgttcgacc tccagccacg gcgcgaccac tgatgtcctc gcactgtcgc
     3901 aaagtgacgt cgctgttctg gccgagacct tccgcctgct gggcgaccaa tcgcgattga
     3961 aaatcctgtt gcagtgcatg cgcggatccg tcgcagtggg cgacattgcc gattcgctag
     4021 acctgtcgca atccttggtc agccatcacc tgcgcctgtt gcgcggcgcg cgcctcgtgc
     4081 gcggcgagcg ccaggccaag cacatcttct acggcatcgc cgaccagcac gtcagccagg
     4141 tactgcagga catggcggtt cacatttcgg aggacagaaa cgacgattga ctgaacgtgt
     4201 gagtgcgcgc cggcctactc cttcaagggg taggcttttt tttggctatc aatctagaga
     4261 aacaccgacg cgccttgctg aatgcctaga accccacata gcccggcaat tcctttccct
     4321 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     4381 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     4441 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     4501 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     4561 gtgcccgtca ctcccactca atagttggca caaagtccta ttctaggcac taccattact
     4621 ttacaacgcc tgaagaaaag aggatatgtt tccttaactg aattacattt acaacttaac
     4681 ccatctcttt gcgaaccgcc gagtacgtga cccgtacgct cggtggtgtg agaggcgcac
     4741 tccgtcagtt actggcggag ccgtctactc gattatgtgc cgttttattc cattttatac
     4801 atttaacaat tgctgaaacg taaagtcggg tttaaacatt tcaatttcgt tttgagttga
     4861 ttcttcttgt gcttctttgc catagataaa catttgctgt tcataatttt caatagcctc
     4921 ttcaatgctg ttaaatttcc cattggtcag attatccgac aatatcaagg catccatcaa
     4981 cccgctgttt acgccttgtc ctgcaaaagg aggcatcaaa tgagcagcat ctccaatcat
     5041 cgttatgggt aatggacgct tacttttcca agacttacct aagggaaata ttcgtgtcgc
     5101 taaccctaca aaagatgatg tcacacgaat cagttctttg tagcgttcgt cccaatcgga
     5161 aaattctttc agaagaaaat caacgacact atttctgttt tgaaaatcta cctgcgtttg
     5221 gtttttccat tcatcaggtg ttttaaaact tattccaaaa tgcaatgcac cattattatt
     5281 aggattcgca aataataaat taccttgatg agcagccatt agccggtttc cattgcatag
     5341 ctgaaaaaat ccaggacagt tcacctctgg atgatgaata tcggcttgta tattgaaagt
     5401 acctgtttct tcaacttccg tgtcggtaac aaattttctt actttagaca ttccaccatt
     5461 ggcaataata accagatctg ctgtttcact cggtttattc tcaaaagtta gtgtccactt
     5521 cttcttacca ggttcaagca taacaagttt tctatcccaa ataaccgtgt cgttttctaa
     5581 actattcaac aagatagccc ttaagtcatt tctgtttatt tcaggattgt caaatcgatt
     5641 ttcgggcttt acattttttg tggataaaat attgcctttt tcatcagcaa tatttacacc
     5701 cattggtaag gctaagtcat aataagtttg taacaatccc gcttttttca ttgcttcctg
     5761 acctgaacct ttgtgtaggt caagggttcc accaaaaatt cttgcctctc ggtcgttgtc
     5821 tctttcgtaa actgaaacgt ctatgccgtt ttgctgtaat aattttgcca tagtcagtcc
     5881 aacgggtcca ccaccaatta ttgcaacgtt cttatcacta agtaaattca tttgtttttc
     5941 tttattgctc atttatatgt ttttcaattc cgtcaattat tgttgtccaa tcttcaaagt
     6001 gcaattcgtg tcctgttcct tcaagagtaa ttaatttcgt attttgtatt ttctcaatta
     6061 atacaccagc gtttttatag tgccaaattt tgtcatctgt tccgtgaatt atcaaggttg
     6121 gttgatttac ttcatttagt ctattccaat attcttcact accttgcaat gtagcgtgat
     6181 tgaacatact tatataattg tttgctctat tgaactcagc tcttatcagc ttttcacttc
     6241 tttgtttatc aaattgtttc ttgccgcaca ttaattctgc accctgaatt aaatagttta
     6301 ccacactgtc ttcatttgac caatcgactg tgcctgcttt accatggaaa tctaaaatac
     6361 gtgtgtccat ctcaggaata gttgggtccg agtctcccca agggcctgta gacataagtg
     6421 ttaatgagct aactctatcg gcaaacttta tagctgctat ttgtgtaatt agcccaccta
     6481 atgacatacc cacaaaatgt gctttgtcta tttcatagcc gtccaaaatt gcaatggcat
     6541 catttgttaa gtcaacaatg tcgtatggtg ctgaacccgg ctcataatta gtggattttc
     6601 ctacatctct gttgtcgtaa cgaataacaa aaaatccttt ttcagataat tgttggcaaa
     6661 attcagtgtc ccaatacagc attgatacgg ttgcacctgc taccaaaagg attgctggat
     6721 ttttcttatt tccaaaactt tccgtacaga gttcaatgcc gtttgtttta attattttct
     6781 ctttcatttc tgttttcctt tgtttgcaac tgattattat cagtcctaaa attaatatcc
     6841 aaagtagttt ttttttcatt tatcaatgtt taactgttat gctggtatct ttcacaatgc
     6901 ccgctaactt atatatatac gaaacattct actctatttc atacccatat ggcaagatag
     6961 gcgaatgttt ttactatttt atttcgcatt ttgaatttct caaatataaa aattatttta
     7021 acattttagt acttatagtg cttaattatt tatgtttcaa atcttatgaa atcatataat
     7081 atataaaaat atatgtatat ataaatatat taaagttgtg tcaaatgtta aaatttaaaa
     7141 ttaacagaaa aaggagttta tagttagtaa aaaatttata acttgggttt tgaaaaagaa
     7201 aatccatgaa aaagaaatta atttatatgt cgatagcgac ctttgctatc gcacagagtt
     7261 ctattgctca gaaccttgat ttacagacac cagcaaacaa tcttaagcaa caaatttcat
     7321 caatatttcc tattgacgta taggaagaat aaacgccctt ttcacccaag tccaacagct
     7381 ttggaccgca gttgactctt tcgacacccc tgcgatgcaa cccaatccgg ctgacgggga
     7441 gccagcaacg ctgaaaattt accctcctct ttcccactag cggctccttt tccgacaacc
     7501 agcacggcgg atccctgccg cggcgctgtg aacgcagcat tttgattggt atcgttggcc
     7561 ttcaggctcg tcagtcaaac agacccagga gcagctcagc cggtggcgcc cggctttcgg
     7621 gtaacgccct ggtcccgctg gtttcggctt tgtgcttcag gtgatcaagg atctgcttga
     7681 tcactatagg gtcttcaatg caggcgatga ctttcatggc gccgccgcag ccgctgcagg
     7741 tctcgatgtc gatattgaaa acacgcttga gccgttgcgc ccatgtcatc gacgctcgcc
     7801 gttgtgctgg tgttgccggt tcatcagcca ccctgacctt gttgcccctg ccccgttttg
     7861 ccggcgtgac caacgcccgg tgccgactgt tgggtgcgaa caccccgtgg aagcgggtta
     7921 ggttgactct gggcttcggt accagggcgg ccagccttgc aatgaaatcc aatggttcga
     7981 aaatgacgtg cgtggtgccg tcccggtacg gcgtcttgag ctggtagcgc acgttgccgc
     8041 ctcgtgttaa cgacagccgc ttctcggata ccgccgggcg gctgatgtac cggcacagcc
     8101 gttcgagctt cttgcgttca tcggccctgg ccgccacgcc ggcgtgcagg ctggacccgg
     8161 ctaccttgcc aatcccgtca ccgaacggat caccactggt cggcagagtt tgcaaagtga
     8221 acacctttcg ccccgcctgt gaaccgacag cgatacggta agtgatcgag tgccccagca
     8281 ggggtgtcat cgggtcgtca tccaccgcat ccgaggccag atagctgttt tcgacatccc
     8341 gttccagcag gccttgccgt tccagatagc gacccacccg gtgggcgatg gtgtgcgtca
     8401 gctgggtgag ctctgggctg gtcggcgcct tgacccagcg gaaacgcgct gagccgtggg
     8461 attgctcgac atacacaccg tcgagaaaca gcatgtggaa gtgaacattc agattgagcg
     8521 ccgatccaaa acgctggatc agggtgaccg cgcccgtctt ggccacttgg tgggtatggc
     8581 ccgctttctt gaccaggtgc gtggcaatga cgcggtaaac gatgcccagc acccacccca
     8641 tgatctcggg ccggctggca aacaggaaac gcagctgaaa cgggaagctc aacacccact
     8701 gacgcatggg ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc
     8761 gccgcgcccc acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct
     8821 cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga
     8881 gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa
     8941 gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc
     9001 ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag
     9061 gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt
     9121 cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact
     9181 cccgtactaa ctgtcacgaa cccctgcaat aactgtcacg cccccctgca ataactgtca
     9241 cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc cctgcaataa
     9301 ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg tgttggaaaa
     9361 atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg
     9421 cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct attcgctgcg
     9481 ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa
     9541 accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt agcggccttt
     9601 ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca
     9661 ttcagcatcg gcatcatctg ctaatttccc gcaatgttcg caggtaatca taggtttgga
     9721 ttggtttctc tatctggatc acacgaattg cagcgactca atgaaccatc atcgtaataa
     9781 tgaagctcct ctccgcattc acagcgcagt tcatcactga gcagttcatc aataaaatca
     9841 gcgtctgtgt aatcgtctag ttttaaatca gtcattattg ctttccttta ttagtttaaa
     9901 ttcaggccgg ctcatccttc tggcgtggcg gcagaccgaa caaggcccgg tcgtggtcgc
     9961 gttcaaggta cgcatccatt gccgccatga gccgatcttc tggccactcg ctgctgttca
    10021 ccttggccag aatcatggcg cccaccagca ccttgcgccg cgtttcgttc ttgcgctctt
    10081 gctgctgctc ccttgccctc acccgctgaa tctcggcatt gatccgtgct cgctgttctt
    10141 cgagcttggc cagcctatcc gccgccttgt tgctcccctt agccatcttg acacctcatt
    10201 gttaatgtgc tgtctcttag gctatcatgg aggcacgacg gcggcaaacc ccgaccgtac
    10261 tttgtacggg agggcgcact taccggtttc tcttcgagaa actggcctaa cggccaccct
    10321 tcgggcggtg cgctctccga gggccattgc atggagccga aaagcaaaag caacagcgag
    10381 gcagcatggc gatttatcac cttacggcga aaaccggcag caggtcgggc ggccaatcgg
    10441 ccaaggccaa ggccgactac atccagcgcg aaggcaagta tgcccgcgac atggatgaag
    10501 tgctgcacgc cgaatccggg cacatgccgg agttcgtcga gcggcccgcc gactactggg
    10561 atgctgccga cctgcatgaa cgcgccaatg ggcggctgtt caaggaggtc gaatttgccc
    10621 tgccggtcga gctgaccctc gaccagcaga aggcgctggc gtccgagttc gcccagcacc
    10681 tgaccggtgc cgaacgtttg ccgtatacgc tggccatcca tgccggtggc ggcgagaacc
    10741 cgcactgcca cctgatgatc tccgagcgga tcaatgacgg catcgagcgg cccgccgctc
    10801 agtggttcaa gcggtacaac ggcaaggccc cggagaaggg cggagcgcag aagaccgaag
    10861 cactcaagcc caaggcatgg cttgagcaga cccgcgaggc atgggccgac catgccaacc
    10921 gggcattaga gcgggctggc cacgacgccc gcattgacca ccgaacgctt gaggcgcagg
    10981 gcatcgagcg cctgcccggt gttcacctgg ggccgaacgt ggtggagatg gaaggccggg
    11041 gcattcgcac cgaccgggca gacgtggccc tgaacatcga caccgccaac gcccagatca
    11101 tcgacttgca ggaataccgg gaggctatag accatgagcg caatcgacag agtgaagaaa
    11161 tccagaggaa tcaacgagtt agcggagcag atcgagccgc tggcccagag catggcgaca
    11221 ctggccgacg aggcccggca ggtcatgagc cagacccagc aggccagcga ggcgcaggca
    11281 gcggagtggc tgaaagccca gcgcgagaca caggcagcat gggccaagct ggccaaggaa
    11341 ttgcgggaag tggccagcga ggtgagcgac gccgcgcaga gcgcccggag cgcggccaga
    11401 gggtggcact ggaagctgtg gctaaccgtg atggcggctt ccatgatgcc tacgctggtg
    11461 ctgctgatcg catcgttgct cttgctcgac ctgacgccac tgacaacaga ggacggctcg
    11521 atctggctgc gcttggtggc ccgatgaaaa acgacagaac cttgcaggcc ataggccgac
    11581 agctcaaggc catgggctgt gagcgcttcg atatcggcgt cagggatgcc accaccggcc
    11641 agatgatgaa ccgggaatgg tcagcctccg aagtgctcca gaacacgcca tggctcaagc
    11701 ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc cgagcaggaa cgccatggtc
    11761 tggtgctggt ggacgacctc agcgagctcg acctggagga catgaaagcc gagggccggg
    11821 aaccggcgct gatcgtggag accagcccga agaactatca ggcatgggtc aaggtggccg
    11881 acgccgcagg cggtgaactc cgggggcaga ttgcccggac gctggccagc gagtacgacg
    11941 ccgacccggc cagcgccgac agccgccact atggccgctt ggcgggtttc accaaccgca
    12001 aggacaagca caccacccgc accggctatc agccgtgggt gctgctgcgt gagtccaagg
    12061 gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc tggccagcag atcgagcagg
    12121 cccagcggca gcaggagaag gcccgcaggc tggccagcct cgaactgccc gagcggcagc
    12181 ttagccgcca ccggcgcacg gcgctggacg agtaccgcag cgagatggcc ggactggtca
    12241 agcgctacgg tgacgacctc agcaagtgcg actttatcgc cgcgcagaaa ctggccagcc
    12301 ggggccgcag tgccgaggaa atcggcaagg ccatggccga ggccagcccg gcgctggcag
    12361 agcgcaagcc cggccacgaa gcggattaca tcgagcgcac cgtcagcaag gttatgggtc
    12421 tgcccagcgt ccagcttgcg cgggccgagc tggcacaggc accggcaccc cgccagcgag
    12481 gcatggacag gggcgggcca gatttcagca tgtaacgctt gcattagcgc taacgggtag
    12541 tatactgtta gctctaatgg aggatttgag atgaaagacc atcgggacac gcagaccggc
    12601 aacctgttgg ccacgcccaa cgcgatcagg caggctaggt ttgccgacgc tcagcgaaaa
    12661 ctgggccgca aggcccgtaa gatatgggcc acagacgacg aggccgaggc gctgcgtctg
    12721 tacctggaag aactcagagc ggcgcagggc gggggtagtg accccgccag cgcctaacca
    12781 ccaactgcct gaacaggagg caatc
//
