LOCUS       NZ_MW598249             5596 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP2 plasmid p5-CR-HvKP2, complete
            sequence.
ACCESSION   NZ_MW598249
VERSION     NZ_MW598249.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yang,X. and Chen,S.
  TITLE     CR-HvKP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yang,X. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2021) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Tat Chee Avenue, Hong Kong,
            Kowloon 999999, Hong Kong
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 11:40:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW598249.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP2"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="p5-CR-HvKP2"
                     /geo_loc_name="China"
                     /lat_lon="30.16 N 120.12 E"
                     /collection_date="2016"
                     /identified_by="Y.XM"
     gene            complement(join(5445..5596,1..358))
                     /locus_tag="KM106_RS00055"
     CDS             complement(join(5445..5596,1..358))
                     /locus_tag="KM106_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<393..1097)
                     /locus_tag="KM106_RS00070"
                     /pseudo=""
     CDS             complement(<393..1097)
                     /locus_tag="KM106_RS00070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1036..1380)
                     /locus_tag="KM106_RS00010"
     CDS             complement(1036..1380)
                     /locus_tag="KM106_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1370..1546)
                     /locus_tag="KM106_RS00015"
     CDS             complement(1370..1546)
                     /locus_tag="KM106_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2186..2344
                     /locus_tag="KM106_RS00075"
     CDS             2186..2344
                     /locus_tag="KM106_RS00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2702..3028
                     /locus_tag="KM106_RS00020"
     CDS             2702..3028
                     /locus_tag="KM106_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            3054..3599
                     /locus_tag="KM106_RS00025"
     CDS             3054..3599
                     /locus_tag="KM106_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3665..4015
                     /locus_tag="KM106_RS00030"
     CDS             3665..4015
                     /locus_tag="KM106_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4085..4351)
                     /locus_tag="KM106_RS00035"
     CDS             complement(4085..4351)
                     /locus_tag="KM106_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4335..4559)
                     /locus_tag="KM106_RS00040"
     CDS             complement(4335..4559)
                     /locus_tag="KM106_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4722..4850
                     /locus_tag="KM106_RS00065"
     CDS             4722..4850
                     /locus_tag="KM106_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(5226..5438)
                     /locus_tag="KM106_RS00045"
     CDS             complement(5226..5438)
                     /locus_tag="KM106_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1633..1709)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1898..2295
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(4..1046)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.713"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5111..5595)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.46e-77"
ORIGIN
        1 gcttcgacag cgctgcgttc tgccggtcga tttcggccag atttgacgct atcaagctcc
       61 cctgatacca caacaccccg ctcaggctgg cgaacagcag tcctgacacc atcgtgatgg
      121 tcagccatgt ccgcccgacc atacgcatca cgctcaggcg gttcgactgc atggccgcgc
      181 tcataccctg ctcgtgggcg cggatggcgg cgctgattct cttcccgctt tcgctcagtt
      241 ccgctctgac agacttctcg tgctcgctga atgcggcttt cagcatttcg ccggtactct
      301 gctgctgcgc tttcgatttc tgctctaagt ccttcgccag cgttaaaaga ctgttcatag
      361 atggctccct taagtcggat gtttcgccct ccgtccgggt cggcaatgct gatgctgctc
      421 tttgtggtgc gcacgacctc aaaacctgcg ctttccagcg cctcagtgac gtcctgacgc
      481 gttttaagct cccctgacga ggcgagggcc agtaagcccc gcgtaatagc ctctgcggct
      541 tcctgcttcg ttttgggtaa cgaagatggc gtcaccagcg cgcgccggtt ctccggtgcg
      601 ttcgggtcgt gcagccccag tcgcccgttc accacggtct gccacgcatc gatgcgcgga
      661 cggtctgccc ggtcgtagta cggctggaga cgcctgccgg tcagcagttc cgtgttcggt
      721 accagaaaat tcagttccag ccgccccttg tcccggtgtt caacccacag cacgctgtac
      781 tggtctttat cgagtccggg catcagaacc cgctcgaagc tcgccatcag cttttcacgc
      841 tggccgggcg gtaaatcctg ttcagcaaaa gacaggacgc cggaggtgta ctttttggcg
      901 tagggcgagg cgtcgataag ctcccggacc tcctccggct ttccctgcag gacgatggcc
      961 ccgtcgcgct ggcggtcttt ccccagcaga taatccaccg gcccggcacc gccgcctcgc
     1021 cctcgcggat gaaacttaac gatcatcgtc tgcccccttt tccagcacgg catgccgcag
     1081 ccgctcgagt tcggcatcaa tggccatcag cgcggccacc acctgaaccc ggtcagcgcc
     1141 tgaccactgg ccgccgttaa ttttccgggc gatctggttg aggttgttcc ccatcccggc
     1201 aagctggcgc agcaaaaccg gatcgatgga cggcagccgc aacgaccgcg ccgggcgcgt
     1261 atcgagacag gtttcccgca tccacaccgc cagttgcctt ccattgcagc gctccagcag
     1321 ctgctggtac tccccatcgg tcacccggat acctaaaaaa cgggtacgct taaccgccat
     1381 cctctcctcc gatatccgca aaatgtgacc gggctgaacg ctcaagctct gccatctgct
     1441 cccggcggta atcttcctcc tgtgcctgac gcatcagatt tgccatcact gccggatgcc
     1501 aggatctttt ttttacgacg agcggcttct tcttcggctt ctgcattatc cctccttcgc
     1561 aaacgccgga cggcacggaa aagttttccg ggtcgctccg gcgttggaca agcgccagcg
     1621 cgtcagtggt gttagcgggt ttcggggcgc agccctgaac cagtcacagc gtttacgctg
     1681 tgtatactgg cttactatgt tggcatgaga gcacgattca tgaaagtgcc tcatgtggca
     1741 ggtgtgatat gtggctcagg agcgtccgca ataccatgca cggacagatg aaatgacgcc
     1801 tcctcgctca ctcggtagct gcgctcctgc cgttcggctg cggcgggcgt gaccgactca
     1861 cggataacgg gataaagcaa aggcgctcaa atcagaaagg gccggagaaa aggatgcggc
     1921 gttgccgttt ttccataggc tccgcctccc ctgacgagca tcacagaaat ggacgctcaa
     1981 gtcagaggag gacgaaaccc gacaggactt aaagatgccg gtgatttctc tccggcggct
     2041 ccctcgtgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc cgcctttttc
     2101 cctttcggga agcgtggcgc tttctcatag ctcacgctgc tggtatctcg gctcggttta
     2161 ggtcgttcgc tccaagctgg gctgtatgca cgaacccccc gttcagcccg actgctgcgc
     2221 cctttccggt aactatcatc ttgagtccaa cccggacgga cacgcaaaaa cgccactggc
     2281 agcagccatt ggtaactgga tatatacgag agagatattg agaggtcttg aagtgggtgc
     2341 ctaactgcgg ctacactgaa agaacagttt tggtatctgc ggtctcgtat acccagttac
     2401 ccggttaagc agttccccaa ctgacttaac cttcgatcaa accgcctccc caggcggttt
     2461 tttcgtttac agtgcaagag attacgcgca gaaaaaaagg atctcaagaa gatcctttaa
     2521 atgatcgtca gttctgttat ccccggagct ccatccatcc cacggaaaaa cacccgccac
     2581 tgatgccctc agcgcactgc acatcatgta caaaccactc cctccctcct tttttattgc
     2641 gtttcttaat gcgcaatggt agctttgtgc gcaacaaaaa acgcaatagc aagggggggt
     2701 aatgagagaa agaacaaata tcatgctaga tataggatta aagcaatcct tacaacggct
     2761 ttcgaataaa accaaaatga gtctaagtga tcttattaac cagagattat caggttcact
     2821 ttcagttgaa gaacgcattg gcgaaaagcc tgaatggatg agaaaccttg atccctatct
     2881 cgccaatcac ttttcaaata ttgagttacc gctcccttca ccattaaaag aataccaaga
     2941 aaaaatggat gagatagatt caattattaa tgacatgatg gttctaaagg aatatttcag
     3001 agctaagttt tacgacgaaa taaaataggc acgctatacg gaggcggaga attatgtttt
     3061 ttaaacggta tttaacggag aaggaaatat acaaggcagc actcccaatt gttaggcttc
     3121 aagggagagt taacacttca ttacgttttt tatccaaata ctcagatgta aacaaaaatg
     3181 gattaattgc attgcatctt aatttattac attttatctc atacaacata cacggtgtag
     3241 agtctctgaa aatcactcaa gcaggcataa acaaagctgt acaacaaagc cctcacgcag
     3301 atgaaataaa aaatattgcg caggcaatag gacgcaaagg acctgagtac atcgaaggat
     3361 taattagaaa cccatggggg aagaaaaatg ataatccact aatatttata atgagcattg
     3421 aagaaagtat aaaagaaacc cttaactcag gttatatgtt atatagagta gctcccataa
     3481 ctgagggtga agcagctgct ttcatggatg ttcttaaaca attattatta tttgaggata
     3541 attttcgcct ttttggtaga gacttggttt ataaaataga aaaattaaaa atcagataac
     3601 aaagtatggt gaacgaaaaa tgaaagagag gatagtgaaa tgtgtaatac caagattctc
     3661 aactatgtat tggttaattg cgttcctagc atttatattc cccggtcgat cttttgctga
     3721 cggttacacc gcttactacc gatgtaatgg tactcaggaa gcatctctta ctgttaaaga
     3781 taacaaacta agtatggttg taggtctttc taagtttaat aggcaactgg atacttatag
     3841 attcaaagaa aagggaatcg aagtagtaat gcagagagct gcttctgatg ataactctga
     3901 ggccataatg tcttttatta tgcctgacgt taacaatatg accttattag ttaaaaataa
     3961 cggctacatt atcgttaaga caacctgctg gttaactaaa accgacaaac agtagaacac
     4021 gtagttagtc gagtatttac cataatacct gttgtcggca tcaaaaaagc ccgcatagcg
     4081 ggcttcagtc gtaaacttcg cggcgatgtc caatcgtggc ggcaaggata acaagctcgt
     4141 catctcggat ctcacacaga acgcgataat ctcccacccg atagcgccag aactcgccca
     4201 gctcaccttt gagcggcttc cctgactggc gaggatcggc agcaactgca atgcgtaaac
     4261 tcataaaatc cacaatccgt cgtgcgttct gtttgtccat cttgcgtaac gatttgagcg
     4321 cccgatcgga atagttaatc gtccagacca agtgacttcc tcacgtcttc agacgaatgc
     4381 actgcttcat caccacggcg aacgcgtgca gcagtttctg ctgaaagata ataatcctcc
     4441 aggtcttcca gatgcgccag tattgcttcc cgcgcataaa acgtctttgt tctgccggtt
     4501 tgcttcgcca gcgagtccag acgggactca atctcatcag ataaccggat agcaagcata
     4561 taaacctccc gaacaccatt tgctatacat gtatatcaac agtaccacaa tcaacctaaa
     4621 agaacaaaaa ataatcttgt tagcccctct tgataatccg taacgcggtc aatatatcta
     4681 gcttgagggc tttcctctta actattgaaa ggataacgat tatgggtctt aagccgggtc
     4741 cgaaacgaat tgccgattca acaggggaac ctgataaacg ccagcgtgac aataaaaaaa
     4801 cgccgggaaa tactgacaaa ttgaaaccca gcaaatcttc aaaaaaataa gtcagtagtt
     4861 aaaagccctc cggggctttt ttgtatctgc cgttcacgct gattaaacat aacacccgtt
     4921 gcccgcaccg ccccggacgg gttcgccgcg aacggggtgg cagtcaggta aaaccggcaa
     4981 caaaccaccc gataaaccgc cccgttcgcg acagataaat tttacctggc tgcatagcca
     5041 tgcagccagg tgaatgacat ccggtttcag cgtaaatgcc cgatgagaac cctgttttcc
     5101 tgcatttcag cccagcgacg gaccgtcata gcttcgctgc cgggaacgcg cttccgtcag
     5161 ttccttttcg tgctgccgtt gctcgtgcgc ccgttctgcg gccacggcca tctcatgacg
     5221 ctgcactacc ggctcaaacg ctgcagttgc ccgctcaagc gttccacctg ctgactcaag
     5281 cgcgtcacgc gctcgctcag cgccgcgttc tcccgttgca taagccctga cattttccgc
     5341 cattccccga aggcgctctc ccactcgtcc agccttttcg agtagtcctg ctgtagctgc
     5401 tctaatgcgc tcagcaactg tttttccagc tcggtcatcg gcacttactc cttcaccagt
     5461 ctcagcgcgt tctgcttcga ccgcccgttc ccgaccgtcc agctctgcgt cctgtctacg
     5521 gccgtcccct tcggcagcac cagaaaacgt ccgttgctgt cttccaggta cgtcacgccc
     5581 caggtcttcg cgttca
//
