LOCUS       NZ_MW598236            11970 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP3 plasmid p4-CR-HvKP3, complete
            sequence.
ACCESSION   NZ_MW598236
VERSION     NZ_MW598236.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Yang,X. and Chen,S.
  TITLE     Transmission of pLVPK-like virulence plasmid in Klebsiella
            pneumoniae mediated by an Incl1 conjugative helper plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Yang,X. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2021) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Tat Chee Avenue, Hong Kong,
            Kowloon 999999, Hong Kong
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; Oxford Nanopore MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/05/2024 11:42:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 14
            CDSs (total)                 :: 14
            Genes (coding)               :: 14
            CDSs (with protein)          :: 14
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW598236.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP3"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="p4-CR-HvKP3"
                     /geo_loc_name="China"
                     /lat_lon="30.16 N 120.12 E"
                     /collection_date="2016"
                     /identified_by="Y.XM"
     gene            811..2775
                     /locus_tag="KM102_RS00005"
     CDS             811..2775
                     /locus_tag="KM102_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2775..3506
                     /gene="mobC"
                     /locus_tag="KM102_RS00010"
     CDS             2775..3506
                     /gene="mobC"
                     /locus_tag="KM102_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3513..4043
                     /locus_tag="KM102_RS00015"
     CDS             3513..4043
                     /locus_tag="KM102_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4070..4249)
                     /locus_tag="KM102_RS00020"
     CDS             complement(4070..4249)
                     /locus_tag="KM102_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4275..4703)
                     /locus_tag="KM102_RS00025"
     CDS             complement(4275..4703)
                     /locus_tag="KM102_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4867..5181)
                     /locus_tag="KM102_RS00030"
     CDS             complement(4867..5181)
                     /locus_tag="KM102_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5169..5447)
                     /locus_tag="KM102_RS00035"
     CDS             complement(5169..5447)
                     /locus_tag="KM102_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5622..5987)
                     /locus_tag="KM102_RS00075"
     CDS             complement(5622..5987)
                     /locus_tag="KM102_RS00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5984..6355)
                     /gene="tolA"
                     /locus_tag="KM102_RS00045"
     CDS             complement(5984..6355)
                     /gene="tolA"
                     /locus_tag="KM102_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6629..6874)
                     /locus_tag="KM102_RS00050"
     CDS             complement(6629..6874)
                     /locus_tag="KM102_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7519..9006
                     /gene="ltrA"
                     /locus_tag="KM102_RS00055"
     CDS             7519..9006
                     /gene="ltrA"
                     /locus_tag="KM102_RS00055"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9111..10796
                     /locus_tag="KM102_RS00060"
     CDS             9111..10796
                     /locus_tag="KM102_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10806..11063
                     /locus_tag="KM102_RS00065"
     CDS             10806..11063
                     /locus_tag="KM102_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            11148..11297
                     /locus_tag="KM102_RS00070"
     CDS             11148..11297
                     /locus_tag="KM102_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             4111..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11401..11970)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.255"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2776..3503
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ttgcctcaca tgttagtccc ctgcttatcc acggaatctg tgggtaactt tgtatgtgtc
       61 cgcagcgccc gccgcagtct cacgcccgga gcgtagcgac cgagtgagcg aggaggcgca
      121 atttccccgg cacctgcaag acggtcaccg gacgcgctga tgtgggatat tgtcccctga
      181 ttaagtcttt ctattccctg acgatcaatt atcaggatag gtgaaccatg tcggtttttc
      241 gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttgcgga gaaatgcaag
      301 tcatccccgc tggggcgtgg ctgacttcag gggaaaagac cttgcttacg ctggctggac
      361 gccaccgacg cacggtcttt tcccctcttt ctggctctgt ccggctggac gccggccacg
      421 ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa atccacggtg
      481 ctggacgcac ctgacgaagc agcaaagctg ctcctgattt tgcgggtggt cagccgctcg
      541 ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt
      601 ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt ttccgcatgg
      661 ggcggaaaga ggctggcctc cgcggtgaaa gctgaacgca atcaccacgg acgtgaccgc
      721 cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg ggggctgcgc atcacgccgg
      781 taaacccgtc gtctgaagga gagagattgc atgtttaata cggattcgct tgcctggcag
      841 tggcatatcc cggagctggc ccggcagatt tacccgtggg tcagctggct gtaccgggag
      901 tattacagtc tggccggttt ttttctggtt atcggcctgt ttttcggtct ggctgaactc
      961 tgggccggcc gaatgcgttt tgtggggctg agccataagg cgcggcggga gtatgccgat
     1021 cgctataagc gcgataataa ccggactgtg ccattttcgg tgctggcctc gctggattca
     1081 cccgggcgga tggtcagggc acggcgacat gtctggaact acgcgtactg gctgaccggc
     1141 gctgccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga gagctgggga
     1201 gaaaccgtcc tgcgcccgga cctctatgcg aacgcagatt attacctgct gcagccggct
     1261 aacctgttcg ggatagcctg gtgtgccgga gggcttgtcg ccggcgtcct tctggcgcgg
     1321 ctgctggcgt tcctgctgct tgatggtcat tttgcggccg ctgacgaggc ggtgaacgcg
     1381 aaactgaatc aggagagccg gcgcagcagc cagcggaccg gagaaatgac agacgtccgc
     1441 cacctgcatt tcggggagcc ggtaccggtt aacgcgctgg ccgatttttc cacggagcag
     1501 gcccggaaac agcaggcggt gtttctgggt aaggatgagc agggccagcc ggtgctggtc
     1561 cctcgtgata cctggcgcaa gaccaatatt cagatcctcg gcctgcccgg gagcggcaag
     1621 agcgcgatgg ccaccaacgc gctcattcgc tgcgtacgcg atttcgggga cgctgtggtg
     1681 tacttcgacc ccaaagggga cgcgtgggcg ccgcacgtct tccgggcgca ctgcccggac
     1741 ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc
     1801 gaccagtacg ccctgaagaa tctgctggtt gccgggttta acctgagtga gaccggcgac
     1861 gtggcggacc actaccgcat cagcgagcag aaagcggcga agctgattgc ggagcagttc
     1921 ccgcaggggg caaacattca gcaggtgctg gcggcggcgt acgcgttgcc ggaggccctg
     1981 aaaaaggacg tgaaggggct gatcaccaag ctggagaacg tggccgacct gagcgtcctg
     2041 cagacggaca gcggtatcga tgtggcgggg ataatcaacg gcggcggctg cctgtacgtc
     2101 atcggctcga tggacgatga ggcggtgatc cgggtgcaga agatgctgtt tgcccgctgc
     2161 gcgcaaatca tcattgcgcg ggatgagttc cggaggtggc cgcacgcgag catcatgctc
     2221 gatgagatta aatatctgct gtcgaagtac gtgctgaacg cgctggggac gctgcgcagc
     2281 cgggactgca acctgctgct cgcgcaccag tcgctgggcg acttcggtca gtgcgggcag
     2341 gatctgcccg ctgattttgt gaaaaccacg gtactggata acacgccgat ccgctggttc
     2401 tatcgcgcgg ccagccagga gtccgcgcag tgggcggcgg gccagaccgg ggagatccgg
     2461 gtggacgtgg agcgccgccg ggccagccgg gaggcgggga acgtggagca catcagcggc
     2521 gacagcttca tccagaagga ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg
     2581 cctgacgggt tcgcggtgat gaccgggctg ggcgtggcgc ggctggcgtt cagcagcccg
     2641 ctgcgggttg agcgccggga aatttcgctg aaaaccttcc cggtgctggc gaaaacggac
     2701 ccgctggcgg agtacctgcc tccggccgac ggcggcggac caaaagcgga tgatgatttt
     2761 gcggggcttt actgatgaca ctggagcagt acaacgtcag ccacgcgaag cggcaggcgc
     2821 ggaacgcgga gaaaacacgg ctgaccctgc gctggctgcg ggaagagctg tgttccacgg
     2881 cggagctggt tgcgcgacgg ctggggattg cggcggtaca gccggtttac cgcttcctgg
     2941 acagtctggt ggcgaaaggg ctgctggtcc gggcgaaata cccggttgac ggccgtcagg
     3001 tcagcgtgtg ggggctgacg ccgcacgggg tggcgttctc gttcgacgag gacgagccgc
     3061 tgacggacgt tattccgttt cagccgtcaa gggtgtcggc ggcgcagctg ccgcaccggc
     3121 tggccgtgca gtccctgcgc ctctccatgg agggccgggg ggccaccggc tggcggtacc
     3181 tgcaccgtat ggccctgaaa gggatgaaag tgccggacgc gctggcggag ctggacggca
     3241 gaacggtggc atttgaggta gagcgcaccg tcaagagccg caggcggtat caggaagtgg
     3301 tgtccgggta tctgttcaac cgccgggcga acggcatcga tgaaatctgg tacatctgcc
     3361 ctgaccggcc gacgcttgtc cgggtacagc gggcgattct ggcggtggat gaaatcgtga
     3421 atccacagac cggcgaggcc cgcaaaacgg tggagctgga ccgggagagg ctgttcgcgt
     3481 gctttaaatt tatgacaacg gagtgaggga ttatggcgct gtacatggta tttattgagg
     3541 acgtggaaga caaagacggc gagcgctgca ttatgccaac cagcgagcat ctggaagaca
     3601 tggtggtgct cgcctggcag accggggcgt atgttttctg ttttgtcgac cgggcccagc
     3661 atctggcgat gatgaaatcc atcgggatgt atgacgagcg gctcaacagc gacgaaaaag
     3721 ccacgtactt tttcaccggt gaaaatccgc aatgggaacg ggacaaaccc acaggaatga
     3781 agctgtgtga ttacctctat cgaaaggtgg agcggttcgc catcgagaac ggtgtgcagg
     3841 tggttgatgc cgggaagtgc tcaccggagc agattaacgc gatgattgag gtggtcagcg
     3901 agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc ggagctgatg cgggaatata
     3961 acagccgcta ttttaacgtg ccggtgcctg aactgccgga ggacaaaccc gcccgccggt
     4021 ggtggccgtt cggcaaaaaa taacgccagc accggcatgg ccggtgctgt tattcttccc
     4081 cgagcttatc ccgcatcacc tgccagagtg tttccgcatc ctcgtgaagc cgttcgcaca
     4141 tgtcggccgg ggtatccatg tccagctcgt tcagcttttc cagcaacagc agcgactgat
     4201 ccttaatgaa tttagccata ttgagggcag ttttttcctg cttcgtcatg ttctccattc
     4261 ccttcggtaa gttctcagct aaaccagccg gaccagaagc gttttttctt ctgtgtcagt
     4321 tctgttgtca atctgtccac ttcggcctgg agtttccggc gctcatcatc gtgctggcgg
     4381 cgttcttctt cccgggatgt tttatcttcc agcatcaggg tgacagcctg ccggagatcg
     4441 gccagttcct tttgcattgc cagaagctgt tcctgtggga aatttttttg tgtattttca
     4501 cggtgtgaaa tgccgggaat ttgtttctca gggatattca catttccgta tacacggatc
     4561 agttctgaca catccacgac agggttgttt ttcccgtcac gtgacacggt aacctttccc
     4621 tgttttacat gattgtatag cgttcttctg gtaatgcccg cggcttgtgc tgcctgtgaa
     4681 aggttgagca atgttttcgc cactgtatac ccctgatttt atcgggtgtg aaaggtgtgt
     4741 aatttctcac ggtaaatgta tcgctgaatt ttacccgttt ttccgttcag gatgtggttt
     4801 tctgacgcgc tgcgcttgtc aaaccggcga cggccacgca atgaattgcg cccccgtcat
     4861 cggttattag aggctgtctg cccccggata cccggattct ttctgatgcc tgacggtgac
     4921 gatcacaatc tggtccattt cccggtcgtg gcggtagagc atcacatagc cactgtcgcc
     4981 aaacccgatc accagttcct tatattccag cggcagaaac ggcaccgggc ggccaatgtc
     5041 tggcaatgtt ttcagttgct ggatagcccg gacgatcacc tcacctcctt ttctggctgc
     5101 cagccgattt ttggttttga gaaagtcctg aaggcgttgt aaatcgcgct gtgccagcgc
     5161 ggaaatcgtt acctgtggca tggcggagca tcctgctcgt tctcagttcc ccaggtactt
     5221 atccaggctt ccgcttcttc cgcagtaagg tgcagacccg tttcctgata gtgctgccat
     5281 gcggcctgtc cgtcccgcag gtactggctg cgcttctctt cccggtcgat gtattcggtg
     5341 attgcctcca gcattagcgc gtgcgctgag cggtgccggt catcggccag cgttttcagc
     5401 cggtccttaa gttcttcatc gagcctgatg gatgttgctg atgacataaa agcccctttg
     5461 tagtcatatg tattacacat gactacttta atccgtggcg gcgctatttg ccagaaatac
     5521 ggcttttgct gcccattttt cagcttcgct tccactaatc agggctgcta caggctcact
     5581 catcatccag gactattgca gcctgcgggt taaaggacct gttactggcg gcaaccatca
     5641 tcattgagct tttgggtggt gactggtcgc ggatcctgta agccgtcatt tttcagactg
     5701 aattcaaaat ttataacctg tcgcattgcc gggtgccctg cgggatatag ccagtgcctc
     5761 attgccgtca gcgtttcccg aaaaaaaagg tgacagccgg taagcgacac gttgtccact
     5821 cgtccatcgt ccagaatatc aaacgtggcg gtcacctgac cttcgatgcg ttttgctgcg
     5881 gcctgtaccg ggtaggttgg ctgcagtgtg gttacgggtt ttagtggtat cgcatgtacc
     5941 ggagtgagga acatggccgc cagcactgtc agagtaaaag ctttcatggt ctgaaatcca
     6001 gaggggcgtt tttgaatatt tgccaggtat tttcatccgg tgccggcgga attttggcgt
     6061 gggtaacggc tgatattgct gctttacaga gtttcggatc tcctccttca gcaatggcgc
     6121 tgttcagagt gccatcacgt tgtatggata tccgcacggt gcacattttt cccgcatagg
     6181 cctctggttc aaacatctgc atttcaacag cggtcttgat ggccgcagcg tagctactga
     6241 ttttagatga cttatccaga gtataaattt tcggcggctg ggtggtggaa tgcttacagg
     6301 ccgtcaggag taaggggctg cacaatgcca gcaggataaa gggtattaaa cgcatcgggt
     6361 tgatgacctg tcagtaaaaa atgttacatg tatatttgtc actctttacg gccacgttca
     6421 tttacataaa cacggcttat cgtatcgttt ttacaccaaa cggcccgcca tgcagttgcg
     6481 tgcataggca tgcagcgggt tacaatttac cgggcgcgtt tccggcggtt ttccgggtgg
     6541 tttgttgcct gttttatccc gtagccgccg gaaacgccct cagaccgtct gagcggtgcg
     6601 ggcaatgcga cgttatggta aatacccctt accggcggcg ctgtggctgc caggtgaagg
     6661 tggtttccgt agcctcgtat ttctcccgac gcatttcctg cgcccgcctg gcttcacgtt
     6721 cgtcctgaat ggcacgaagc accctttccc gcacctgttg ttttaccgcc tttggcagcg
     6781 ttttgttccc tttcaggccc atggccagtg cctgagcgac aaaggcgtcc gccaggcgct
     6841 tttcttcagc gggagtcagt ttcaggtgcg tcatagtggc ctccgggcgt ctcaggtttc
     6901 ccgaacagtt taaccgcagg tcctctcctc atgccagtta gctttcagat gtgtctatac
     6961 catctgaaat gtaagtgtga tgaatgtctt aaataataaa atttgttgcc atgagaaatt
     7021 ttcgggaaaa gacgcgatct gcttacaggt ggctccgggc gcttttttgt gcctgaagat
     7081 gagaactaat ctcatatatt gacacctgaa aacgggagga gtaaggtaat aattatactg
     7141 tgtatatgta cagtgtgcgc cgggagaccg gttagagatc aaggggtgaa agtccccgac
     7201 cattgaagga ccagcaatcc acaaggtccc cgagtcatgc gttgcatacc gcgaggtatg
     7261 gggcgaagcg ttgacagggg tgttgacagg ccagccattg agccacgaaa tgtatattaa
     7321 attaccgggt gccgacgttg tactgttaac ggaaggcaac atcatagggt gcgatactgc
     7381 gagtgccaca tggacccggc ggggtctgag accctggcat gtcaatacga tctctacgcg
     7441 ggaaccggga gatctcccct ctgaccatct gccagtgtcg gagatggccc gcaccgggaa
     7501 gacgaggagt catagccggt gatgtacgga gaggagaagt cggactcgct catagtagcg
     7561 gcgaagcagg cgaacaaccc gaaaggagcg gagtcagtgg agcgaaggag cggggccaag
     7621 gggaacgcgg aacagccaca catgcgccgg acacagagcc gggaaagcat gtcacagagg
     7681 ctgtcacgcg tgcgggaagc tgcgaagcag cggaagaaag aacggtttac agcattgttc
     7741 cacctgctga cagtcgaagc actggaagcc gcattcctct ccctgagcag gaaagcggcc
     7801 gccggagtgg atggcatcag gtggatggac tacgccggaa acatgaagaa caacataaca
     7861 gatctgcacc ggaggctaca tcagggcagc tacagggcgc agcccggcag gcgtcactac
     7921 atcccaaaag cggatggaaa acaacgcccg ctcggcatcg cctcgctgga ggacaagatc
     7981 gtccagtatg cgctggtgaa aatcctgaac gcagtctatg aaaacgactt tatggggttc
     8041 tcatacgggt tcagacccgg gcgaagccag cacgatgcac tggacgcact ggccacaggg
     8101 ctggtacgca ctaacgtaaa ctgggtactg gatgccgaca tcagtcagtt cttcgacagg
     8161 gtgagccacg aatggctgat caggttcaca gagcatcgga tcggcgaccg gagggtaatc
     8221 aggctcatac gtaagtggct cacagccggg acgtcggagg agggtcaatg gcgagcaacg
     8281 gaggaaggca ccccacaggg tgcggtcatc tcaccgctgc tggcaaacat atacctccac
     8341 tacgtcttcg atctgtgggc gcatcagtgg cgacgtcgct atgccacagg caatgtggta
     8401 atggtcagat acgccgatga catcgtcatc gggttcgaca aacgatacga tgcccggcgc
     8461 ttccgtatag ccatgcagcg cagactgagg gagttcggac tcacggttca cccggagaaa
     8521 acccgtctga tggagttcgg ccgcttcgct gccgaaaacc gtgccatcag gggaaaaggc
     8581 aaaccagaaa cgttcaactt cctcgggttc acgcacatca gcgggaaaga tcgcaacggc
     8641 aggttcatgc tgatacgaaa gacccgccgg gatcggatga cggcaactct gaaagccatc
     8701 aaagacggtc tgcgaaggcg ctggcattac tcaatccccg aacagggaaa atggctcagg
     8761 agagtggttc agggatacct gaactatcac tcggtaccgg gcaacttccc caccatgcag
     8821 aagttcagga cacacgtaac aaacctctgg cgccgggcgc tcaggcgcag gagccagaag
     8881 gatgatacga cctggacgaa agcaaacaaa ctggcagccg catggctacc aagggttcgg
     8941 gttcttcatc catggcctgt ggagcggttc accgccagac acccgaggca ggagcccggt
     9001 gcgtaaatcg cgcacgccgg gatctgtgcg gggggtatcc ggtaacgggt atccctaccg
     9061 cgacatgttg gctttaattt attgttttaa atgtcaaaga ggaaaacggt atgagtggtg
     9121 gagacggtcg aggtccgggt aattcaggtc tgggacataa tggtggtcag gccagtggga
     9181 atgtgaacgg tacgtctggt aaaggtggcc cttcatcagg tggtggtacg gatccaaaca
     9241 gcgggccggg ctggggtacg acgcatacgc cgaatggaga tattcataac tataatccgg
     9301 gggagtttgg taacggcggg agtaaacccg gtggtaatgg tggtaacagc ggcaatcata
     9361 gcggtagctc tggtggcgga cagtcttcgg ccactgcgat ggccttcggt ctgcctgccc
     9421 tggctactcc gggggctgaa ggactggctt tatcagtttc cggcgatgcg ttgtcggccg
     9481 ccgttgctga tgtgctggct gccctgaagg ggccgtttaa gtttggtctg tgggggattg
     9541 cgatctacgg tgtgctgcct tctgagattg caaaagatga tccgaaaatg atgtcaaaaa
     9601 ttatgacgtc attaccggcc gatgcggtga cggagactcc ggcaagtact ttaccactgg
     9661 accaggcgac ggttcgtgtc agacaacggg ttgtggatgt ggtgaaggat gagcggcagc
     9721 atattgcggt tgtcgcaggt cggccaatga gtgtccctgt ggtggatgcg aaaccgacaa
     9781 aacgtccggg ggtattcagt gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac
     9841 cgaaaggtgt tccgacagcg aaagccccgc caaaaggcat tgttgctgaa aaaggtgatt
     9901 cacgtccggc tggttttaca gccggtggta actcccgtga ggccgttatt cgtttcccga
     9961 aagagaccgg acagaagccg gtttatgtgt cggtgacaga tgttcttacc ccggcacagg
    10021 taaaacagcg tcaggaggaa gaaaagcgtc gccagcaggc atgggacgcc gctcatccgg
    10081 aagaggggct gaaaagagac tatgataaag cgaaagctga gctggatgcc gaagataaaa
    10141 atattgcgac cttaaacagc cgcattgcat cgacagagaa ggcgctcccc ggtgcaaggg
    10201 ctgctgttca ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg
    10261 tgacttataa cccgcctcat gaatatggct ccgggtggca ggatcaggtt cgctatcttg
    10321 ataaggatat tcagaatcag aatgagaaat taaaagcggc tcaggcatct ttaaacgcaa
    10381 tgaatgaatc cttatccaga gataaggctg cacttaccgg ggcgatggag agccggaaac
    10441 aaaaggagaa aaaagcgaag gatgcagaaa ataagttaaa tgaggaaaag aacaagcctc
    10501 gcaaggggac taaagactac ggccatgatt atcatccagt acctaaaaca gaagacataa
    10561 agggacttgg agagttaaag cgaggtgatc ctaaaacacc taaacaaggt ggtggtggta
    10621 agcgggcccg atggtatggc gataaaaaac gtaaaattta tgaatgggat tcccagcacg
    10681 gtgagcttga agggtatcgt gccagtgatg gcgaacacct aggcgcattt gatcccaaaa
    10741 cgggtaagca ggttaaaggg ccagatccaa aacggaacat taaaaaatat ctttgagagg
    10801 taaatatggg gcttaaatta cgtttgacat ggtttgataa gaaaactgaa gagtttaaag
    10861 gggaagagta ttctaaagac tttggtgacg atggttctgt cattgaaagc ttgggaatgc
    10921 ctttaaagga taatattaat aatggtgagt ttgatttgaa gaaagaatgg gttcctttac
    10981 tacagccatt ttttaaaaat caaataaaac ctgatgtgga taattgttat atagcttttg
    11041 attatcggga cggtaactgg taacaaaaaa ttatcaagga tgatttgaaa tactgacttc
    11101 cggtgttcag ggatgaatgc cggagtcttt aaatacaggg aaagtatatg aaaaaggtaa
    11161 agactatttt tctttttata ttgattgttg ctggctttct tctggccgca tgtcaggcaa
    11221 attatatccg ggatgttcag ggcggaacgg tggcaccatc gtcctcctct gaactgacgg
    11281 ggatcgcggt tcagtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    11341 aatcttttgc cctgtaaacg aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt
    11401 cagttgggga actgcttaac cgtggtaact ggctttcgca gagcacagca accaaatctg
    11461 tccttccagt gtagccggac tttggcgcac acttcaagag caaccgcgtg tttagctaaa
    11521 caaatcctct gcgaactccc agttaccaat ggctgctgcc agtggcgttt tgcgtgcttt
    11581 cccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg ctgaacgggg
    11641 agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag ataccagtgt
    11701 gtgagctatg agaaagcgcc acacttcccg caagggagaa cggcggacag gtatccggta
    11761 aacggcaggg tcggaacagg agagcgcaag agggagcgac ctaccggaaa cggtggggat
    11821 ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc tcacggctcc
    11881 cacagatgca ccgaaaaagc gtctgtttat gtgaactctg gcaggagggc ggagcctatg
    11941 gaaaaacgcc accggcgcgg ccctgccgtt
//
