LOCUS       NZ_MW598235             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP3 plasmid p5-CR-HvKP3, complete
            sequence.
ACCESSION   NZ_MW598235
VERSION     NZ_MW598235.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yang,X. and Chen,S.
  TITLE     Transmission of pLVPK-like virulence plasmid in Klebsiella
            pneumoniae mediated by an Incl1 conjugative helper plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yang,X. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2021) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Tat Chee Avenue, Hong Kong,
            Kowloon 999999, Hong Kong
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:18:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW598235.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP3"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="p5-CR-HvKP3"
                     /geo_loc_name="China"
                     /lat_lon="30.16 N 120.12 E"
                     /collection_date="2016"
                     /identified_by="Y.XM"
     gene            complement(join(5551..5596,1..500))
                     /locus_tag="KM104_RS00005"
     CDS             complement(join(5551..5596,1..500))
                     /locus_tag="KM104_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(526..852)
                     /locus_tag="KM104_RS00010"
     CDS             complement(526..852)
                     /locus_tag="KM104_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1210..1368)
                     /locus_tag="KM104_RS00065"
     CDS             complement(1210..1368)
                     /locus_tag="KM104_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2008..2184
                     /locus_tag="KM104_RS00015"
     CDS             2008..2184
                     /locus_tag="KM104_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2174..2518
                     /locus_tag="KM104_RS00020"
     CDS             2174..2518
                     /locus_tag="KM104_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2727..>3161
                     /locus_tag="KM104_RS00055"
                     /pseudo=""
     CDS             2727..>3161
                     /locus_tag="KM104_RS00055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3196..3705
                     /locus_tag="KM104_RS00030"
     CDS             3196..3705
                     /locus_tag="KM104_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3712..3924
                     /locus_tag="KM104_RS00035"
     CDS             3712..3924
                     /locus_tag="KM104_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4300..4428)
                     /locus_tag="KM104_RS00060"
     CDS             complement(4300..4428)
                     /locus_tag="KM104_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            4591..4815
                     /locus_tag="KM104_RS00040"
     CDS             4591..4815
                     /locus_tag="KM104_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4799..5065
                     /locus_tag="KM104_RS00045"
     CDS             4799..5065
                     /locus_tag="KM104_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5135..5485)
                     /locus_tag="KM104_RS00050"
     CDS             complement(5135..5485)
                     /locus_tag="KM104_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1843..1922
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1260..1657)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2509..4040
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaggcgaa aattatcctc aaataataat aattgtttaa gaacatccat gaaagcagct
       61 gcttcaccct cagttatggg agctactcta tataacatat aacctgagtt aagggtttct
      121 tttatacttt cttcaatgct cattataaat attagtggat tatcattttt cttcccccat
      181 gggtttctaa ttaatccttc gatgtactca ggtcctttgc gtcctattgc ctgcgcaata
      241 ttttttattt catctgcgtg agggctttgt tgtacagctt tgtttatgcc tgcttgagtg
      301 attttcagag actctacacc gtgtatgttg tatgagataa aatgtaataa attaagatgc
      361 aatgcaatta atccattttt gtttacatct gagtatttgg ataaaaaacg taatgaagtg
      421 ttaactctcc cttgaagcct aacaattggg agtgctgcct tgtatatttc cttctccgtt
      481 aaataccgtt taaaaaacat aattctccgc ctccgtatag cgtgcctatt ttatttcgtc
      541 gtaaaactta gctctgaaat attcctttag aaccatcatg tcattaataa ttgaatctat
      601 ctcatccatt ttttcttggt attcttttaa tggtgaaggg agcggtaact caatatttga
      661 aaagtgattg gcgagatagg gatcaaggtt tctcatccat tcaggctttt cgccaatgcg
      721 ttcttcaact gaaagtgaac ctgataatct ctggttaata agatcactta gactcatttt
      781 ggttttattc gaaagccgtt gtaaggattg ctttaatcct atatctagca tgatatttgt
      841 tctttctctc attacccccc cttgctattg cgttttttgt tgcgcacaaa gctaccattg
      901 cgcattaaga aacgcaataa aaaaggaggg agggagtggt ttgtacatga tgtgcagtgc
      961 gctgagggca tcagtggcgg gtgtttttcc gtgggatgga tggagctccg gggataacag
     1021 aactgacgat catttaaagg atcttcttga gatccttttt ttctgcgcgt aatctcttgc
     1081 actgtaaacg aaaaaaccgc ctggggaggc ggtttgatcg aaggttaagt cagttgggga
     1141 actgcttaac cgggtaactg ggtatacgag accgcagata ccaaaactgt tctttcagtg
     1201 tagccgcagt taggcaccca cttcaagacc tctcaatatc tctctcgtat atatccagtt
     1261 accaatggct gctgccagtg gcgtttttgc gtgtccgtcc gggttggact caagatgata
     1321 gttaccggaa agggcgcagc agtcgggctg aacggggggt tcgtgcatac agcccagctt
     1381 ggagcgaacg acctaaaccg agccgagata ccagcagcgt gagctatgag aaagcgccac
     1441 gcttcccgaa agggaaaaag gcggaacagg tatccggtaa acggcagggt cggaacagga
     1501 gagcgcacga gggagccgcc ggagagaaat caccggcatc tttaagtcct gtcgggtttc
     1561 gtcctcctct gacttgagcg tccatttctg tgatgctcgt caggggaggc ggagcctatg
     1621 gaaaaacggc aacgccgcat ccttttctcc ggccctttct gatttgagcg cctttgcttt
     1681 atcccgttat ccgtgagtcg gtcacgcccg ccgcagccga acggcaggag cgcagctacc
     1741 gagtgagcga ggaggcgtca tttcatctgt ccgtgcatgg tattgcggac gctcctgagc
     1801 cacatatcac acctgccaca tgaggcactt tcatgaatcg tgctctcatg ccaacatagt
     1861 aagccagtat acacagcgta aacgctgtga ctggttcagg gctgcgcccc gaaacccgct
     1921 aacaccactg acgcgctggc gcttgtccaa cgccggagcg acccggaaaa cttttccgtg
     1981 ccgtccggcg tttgcgaagg agggataatg cagaagccga agaagaagcc gctcgtcgta
     2041 aaaaaaagat cctggcatcc ggcagtgatg gcaaatctga tgcgtcaggc acaggaggaa
     2101 gattaccgcc gggagcagat ggcagagctt gagcgttcag cccggtcaca ttttgcggat
     2161 atcggaggag aggatggcgg ttaagcgtac ccgtttttta ggtatccggg tgaccgatgg
     2221 ggagtaccag cagctgctgg agcgctgcaa tggaaggcaa ctggcggtgt ggatgcggga
     2281 aacctgtctc gatacgcgcc cggcgcggtc gttgcggctg ccgtccatcg atccggtttt
     2341 gctgcgccag cttgccggga tggggaacaa cctcaaccag atcgcccgga aaattaacgg
     2401 cggccagtgg tcaggcgctg accgggttca ggtggtggcc gcgctgatgg ccattgatgc
     2461 cgaactcgag cggctgcggc atgccgtgct ggaaaagggg gcagacgatg atcgttaagt
     2521 ttcatccgcg agggcgaggc ggcggtgccg ggccggtgga ttatctgctg gggaaagacc
     2581 gccagcgcga cggggccatc gtcctgcagg gaaagccgga ggaggtccgg gagcttatcg
     2641 acgcctcgcc ctacgccaaa aagtacacct ccggcgtcct gtcttttgct gaacaggatt
     2701 taccgcccgg ccagcgtgaa aagctgatgg cgagcttcga gcgggttctg atgcccggac
     2761 tcgataaaga ccagtacagc gtgctgtggg ttgaacaccg ggacaagggg cggctggaac
     2821 tgaattttct ggtaccgaac acggaactgc tgaccggcag gcgtctccag ccgtactacg
     2881 accgggcaga ccgtccgcgc atcgatgcgt ggcagaccgt ggtgaacggg cgactggggc
     2941 tgcacgaccc gaacgcaccg gagaaccggc gcgcgctggt gacgccatct tcgttaccca
     3001 aaacgaagca ggaagccgca gaggctatta cgcggggctt actggccctc gcctcgtcag
     3061 gggagcttaa aacgcgtcag gacgtcactg aggcgctgga aagcgcaggt tttgaggtcg
     3121 tgcgcaccac aaagagcagc atcagcattg ccgacccgga cggagggcga aacatccgac
     3181 ttaagggagc catctatgaa cagtctttta acgctggcga aggacttaga gcagaaatcg
     3241 aaagcgcagc agcagagtac cggcgaaatg ctgaaagccg cattcagcga gcacgagaag
     3301 tctgtcagag cggaactgag cgaaagcggg aagagaatca gcgccgccat ccgcgcccac
     3361 gagcagggta tgagcgcggc catgcagtcg aaccgcctga gcgtgatgcg tatggtcggg
     3421 cggacatggc tgaccatcac gatggtgtca ggactgctgt tcgccagcct gagcggggtg
     3481 ttgtggtatc aggggagctt gatagcgtca aatctggccg aaatcgaccg gcagaacgca
     3541 gcgctgtcga agctgaacgc gaagacctgg ggcgtgacgt acctggaaga cagcaacgga
     3601 cgttttctgg tgctgccgaa ggggacggcc gtagacagga cgcagagctg gacggtcggg
     3661 aacgggcggt cgaagcagaa cgcgctgaga ctggtgaagg agtaagtgcc gatgaccgag
     3721 ctggaaaaac agttgctgag cgcattagag cagctacagc aggactactc gaaaaggctg
     3781 gacgagtggg agagcgcctt cggggaatgg cggaaaatgt cagggcttat gcaacgggag
     3841 aacgcggcgc tgagcgagcg cgtgacgcgc ttgagtcagc aggtggaacg cttgagcggg
     3901 caactgcagc gtttgagccg gtagtgcagc gtcatgagat ggccgtggcc gcagaacggg
     3961 cgcacgagca acggcagcac gaaaaggaac tgacggaagc gcgttcccgg cagcgaagct
     4021 atgacggtcc gtcgctgggc tgaaatgcag gaaaacaggg ttctcatcgg gcatttacgc
     4081 tgaaaccgga tgtcattcac ctggctgcat ggctatgcag ccaggtaaaa tttatctgtc
     4141 gcgaacgggg cggtttatcg ggtggtttgt tgccggtttt acctgactgc caccccgttc
     4201 gcggcgaacc cgtccggggc ggtgcgggca acgggtgtta tgtttaatca gcgtgaacgg
     4261 cagatacaaa aaagccccgg agggctttta actactgact tatttttttg aagatttgct
     4321 gggtttcaat ttgtcagtat ttcccggcgt ttttttattg tcacgctggc gtttatcagg
     4381 ttcccctgtt gaatcggcaa ttcgtttcgg acccggctta agacccataa tcgttatcct
     4441 ttcaatagtt aagaggaaag ccctcaagct agatatattg accgcgttac ggattatcaa
     4501 gaggggctaa caagattatt ttttgttctt ttaggttgat tgtggtactg ttgatataca
     4561 tgtatagcaa atggtgttcg ggaggtttat atgcttgcta tccggttatc tgatgagatt
     4621 gagtcccgtc tggactcgct ggcgaagcaa accggcagaa caaagacgtt ttatgcgcgg
     4681 gaagcaatac tggcgcatct ggaagacctg gaggattatt atctttcagc agaaactgct
     4741 gcacgcgttc gccgtggtga tgaagcagtg cattcgtctg aagacgtgag gaagtcactt
     4801 ggtctggacg attaactatt ccgatcgggc gctcaaatcg ttacgcaaga tggacaaaca
     4861 gaacgcacga cggattgtgg attttatgag tttacgcatt gcagttgctg ccgatcctcg
     4921 ccagtcaggg aagccgctca aaggtgagct gggcgagttc tggcgctatc gggtgggaga
     4981 ttatcgcgtt ctgtgtgaga tccgagatga cgagcttgtt atccttgccg ccacgattgg
     5041 acatcgccgc gaagtttacg actgaagccc gctatgcggg cttttttgat gccgacaaca
     5101 ggtattatgg taaatactcg actaactacg tgttctactg tttgtcggtt ttagttaacc
     5161 agcaggttgt cttaacgata atgtagccgt tatttttaac taataaggtc atattgttaa
     5221 cgtcaggcat aataaaagac attatggcct cagagttatc atcagaagca gctctctgca
     5281 ttactacttc gattcccttt tctttgaatc tataagtatc cagttgccta ttaaacttag
     5341 aaagacctac aaccatactt agtttgttat ctttaacagt aagagatgct tcctgagtac
     5401 cattacatcg gtagtaagcg gtgtaaccgt cagcaaaaga tcgaccgggg aatataaatg
     5461 ctaggaacgc aattaaccaa tacatagttg agaatcttgg tattacacat ttcactatcc
     5521 tctctttcat ttttcgttca ccatactttg ttatctgatt tttaattttt ctattttata
     5581 aaccaagtct ctacca
//
