LOCUS       NZ_MW388915             8331 bp    DNA     circular CON 18-JUL-2023
DEFINITION  Bacteroides fragilis strain Q8 plasmid pBFQ8b, complete sequence.
ACCESSION   NZ_MW388915
VERSION     NZ_MW388915.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Baaity,Z., Jamal,W., Rotimi,V.O., Burian,K., Leitsch,D.,
            Somogyvari,F., Nagy,E. and Soki,J.
  TITLE     Molecular characterization of metronidazole resistant Bacteroides
            strains from Kuwait
  JOURNAL   Anaerobe 69, 102357 (2021)
   PUBMED   33713801
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Zain,B., Jamal,W., Rotimi,V.O., Burian,K., Somogyvari,F., Nagy,E.
            and Soki,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2020) Institute of Clinical Microbiology,
            University of Szeged, Semmelweis 6, Szeged H-6725, Hungary
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Lasergene v. 16
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/18/2023 03:06:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW388915.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="Q8"
                     /db_xref="taxon:817"
                     /plasmid="pBFQ8b"
     gene            312..914
                     /locus_tag="KBP66_RS00005"
     CDS             312..914
                     /locus_tag="KBP66_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_005812751.1"
                     /translation="MEKDSKTTVAVERTTFTKLDRLAKANNVSKMEFLTHAINYFEKYG
                     INPVEHESPAQEMQKLIKRMDQVFAFLKKQETDLVRPACEALAGASTQITISLSSLLSE
                     EKFRRFLKENKELLAEIINSSDNNGKAIGRTERAVRDSDSTIRKNQDIIFKRIEASETA
                     QIKAYKHIVSFLDAKGKTGLLDDINKVYEREKERRGL"
                     /name="BfmA/BtgA family mobilization protein"
     gene            911..1810
                     /locus_tag="KBP66_RS00010"
     CDS             911..1810
                     /locus_tag="KBP66_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="WP_005812749.1"
                     /translation="MNIKIQGGGSGTYANTGSCTGVTTYLQHEDLERMKNGQEVQPFFN
                     NSRDYISAQEVTFKIDNNKAKLSRNDAKFYVITVSPSSRELEKMGKTEKEQAEAMRKYV
                     RDDVMQHYAEGFGKGLNKEDIEYYGKIHFERKGADRYDMHAHIIVSRKDRSNTRKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCETSFDKRTGYDRAPEQTFDYLNTMKNGSPKEIFQ
                     KKEWAERVNHERLEKMKAEWSRDLQELHQEQGREESQQQGNSISQVPDINQAPQRKKQQ
                     EEEIDQPRKRSRGFGMGM"
                     /name="DUF5712 family protein"
     gene            complement(2334..2846)
                     /locus_tag="KBP66_RS00015"
     CDS             complement(2334..2846)
                     /locus_tag="KBP66_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-nitroimidazole reductase NimE"
                     /protein_id="WP_005812773.1"
                     /translation="MFREMRRKRQLLPQEESVAILEKMTNGTLALHGDNGYPYAVPLSY
                     FYADGKIYFHCAKIGHKVDAIMQNNKVSFCVVEQDNIKPAEFTTYFRSVIVFGKAYILT
                     DETEKRMAMTLLVNKYSFGEPGLSDEIAKSINHLLMVKIDIEHMTGKEAIDLVREKEKF
                     CTSEKTS"
                     /name="5-nitroimidazole reductase NimE"
     gene            2957..3814
                     /locus_tag="KBP66_RS00020"
     CDS             2957..3814
                     /locus_tag="KBP66_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491447.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISBf6 family transposase"
                     /protein_id="WP_032491447.1"
                     /translation="MVTKLIYSESPMYTMLDKDTIKNEILPHLSKAKRGYATQSCLIEV
                     VNAILYKLKTGCQWAFLPVEALFSGKVLSYGAVYHHYNKWSKSGEWKSMWVRLLDKHRH
                     KLDMSSVDLDGSHTAALRGGEEVGYQGRKKRKTTNALYLTDRQGLPIAMSSPKSGEHHD
                     VHDIEKVIGDMFNDLEKSHIRVDGLFLNADAGFDCDVLRGFLGEKEIIANICINKRREN
                     ADDIFVDDELYAERYSIERTNAWMDSFRTLLNRFDTTVSSWMSWNYLAFAVLLLKKISR
                     KQKV"
                     /name="IS5-like element ISBf6 family transposase"
     gene            complement(4342..>4563)
                     /locus_tag="KBP66_RS00025"
                     /pseudo=""
     CDS             complement(4342..>4563)
                     /locus_tag="KBP66_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4766..5296)
                     /locus_tag="KBP66_RS00030"
     CDS             complement(4766..5296)
                     /locus_tag="KBP66_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_210203692.1"
                     /translation="MKKNEFRKELVLILVLLCTTIAYAQKNDSSFMVAPADSTKDISLT
                     QRVDSLEHELSYIKLSYELYTLNNDLSIFXNDIGVRTNAIQINIYTHNFDYKLYKAYKD
                     NYEATKDKLLSYQDLIETKKTYFWLKIITYPYTEVEKKLLLSSYMTIDKGYSQLESSLK
                     LMKICLDMYKDSL"
                     /name="hypothetical protein"
     gene            complement(5307..5849)
                     /locus_tag="KBP66_RS00035"
     CDS             complement(5307..5849)
                     /locus_tag="KBP66_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_005812766.1"
                     /translation="MTDKVTIQPKSKPEKTTISISGETSERLEIYCRANGILRKEFVSL
                     ALEYFERTGFDLKSNALDYSPLEKIIGELNEVKATMQTSNEGTEAIRQLLQAVREQTAK
                     QLPAPELIAKAAEEKVRAEMKSEEQVKELQVLQEENDRLRNEIKALQEYKEKAHRELCR
                     VRDEQKTFGKIKVNTEL"
                     /name="BfmA/BtgA family mobilization protein"
     gene            complement(5963..6232)
                     /locus_tag="KBP66_RS00040"
     CDS             complement(5963..6232)
                     /locus_tag="KBP66_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_253713766.1"
                     /translation="MPSREKLKIERNFESVTKSKSNDMKEGDLILVSAEATGLGKPMEA
                     VIDKIETFMGQTLVTVTYTQPDALSGFGGCFVDSHITLSEEKAK"
                     /name="hypothetical protein"
     gene            complement(6192..7172)
                     /locus_tag="KBP66_RS00045"
     CDS             complement(6192..7172)
                     /locus_tag="KBP66_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812759.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032536519.1"
                     /translation="MAIVNKDLIQSYIMTTAKYDFNVYEKRIIYRLVEMAQGELQGIKF
                     ADNCQKIEHDLFSCVNITMPIACLLNGEDDKNYARVKEALQSLQRKIFEYEDDEIWQSI
                     SIIALPYIRKRSSVLSFQVHPRVWDCILDFSKGFRKYELKAAMSFKSQFSMRFYELLSN
                     QKTPLIYSIEQLKEMFCVADKYERINDFMRKVVEAAKKELDEVSPYTFEYTPLKTGRKI
                     TAIKFYPVYQPEHRDSDLEKHDLQKQTALSWSLAPEVRSYLKNSIEFSDKEIKNNLDLF
                     ISAQTLLPDLLNELAILKGKSREKKNPKGYIINAIKGKIKDRKKL"
                     /name="replication initiation protein"
     gene            7463..7891
                     /locus_tag="KBP66_RS00060"
     CDS             7463..7891
                     /locus_tag="KBP66_RS00060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_253713767.1"
                     /translation="MKNDFLNVCESKAKASAAFFEERTFRACTQISTQNFCFIGFYPYF
                     CSVNQYIRTMQIVTTREFRANQKKYFEMAETETILVSRRNAAPIMVCAVREGDFPSKEE
                     LAAIQRGIEDIRNGNTFRMAKNESLDDFLNRIEGEGNV"
                     /name="hypothetical protein"
     gene            7884..8153
                     /locus_tag="KBP66_RS00055"
     CDS             7884..8153
                     /locus_tag="KBP66_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671563.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_032536520.1"
                     /translation="MYEIEFSKEAVKHVLLMRKSSPQLFRKLEKLLDELREHPYTGTGH
                     PEQLRYLEGVWSRQLDKKNRIRYMVNETTVIVFIISAWGHYDDK"
                     /name="Txe/YoeB family addiction module toxin"
     ARG             complement(2338..2846)
                     /name="nimE"
                     /gene="nimE"
                     /gene_name="5-nitroimidazole reductase NimE"
                     /tool="amrfinderplus"
                     /drug_class="NITROIMIDAZOLE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6193..7172)
                     /name="rep_cluster_1079"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.068"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          912..1807
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.993"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgttgtgcg gttttgttgg gtatttctgt tgtgtaccgg gcgtgcggtt ttcgtgtgtg
       61 tttacgtgtg tgggataggt tctatctctc cccgtcccct ttgctaattt cgcgctgggg
      121 agggtgttcg atagaacagc aagaggaaac cgggcaagcc ctgttttttc ctcttgmcct
      181 ackgagtttt tagctgtaaa tcagttattt agaaaattat actaatataa tttggttata
      241 atgaataaat atactatctt gtaaaccaat aagttagtat attaattcat tataataagc
      301 cttataatat catggaaaaa gacagcaaaa caacggtagc agtagaacgt actactttta
      361 ctaaacttga ccgtctggcc aaggcaaaca atgtgtcaaa aatggagttc cttacacatg
      421 ctataaacta ctttgagaag tacggtataa acccggtcga gcatgaaagc ccagcacagg
      481 aaatgcagaa acttatcaag cgcatggatc aggtgttcgc attcctgaag aaacaggaaa
      541 cggatcttgt gcgtccggct tgtgaagctc tggccggagc cagcacacag ataaccataa
      601 gcctttcatc cttactttcg gaagaaaagt tcaggcggtt cctgaaggag aacaaggaac
      661 tgcttgccga gattatcaac agttccgaca acaacggcaa ggccataggc cggacagaaa
      721 gggcggtgcg ggattccgat tcaactatca ggaagaacca ggatattatc ttcaagcgga
      781 tagaagccag cgaaacggca cagattaaag cctataagca tattgtttcc ttcctcgatg
      841 caaaaggaaa gaccgggctg ctcgatgata taaacaaggt ctatgagagg gaaaaggaaa
      901 ggaggggact atgaacataa agattcaggg tggcggttcc ggaacctatg ccaatacagg
      961 gagctgcaca ggagtaacga cctacctcca gcatgaggat ctggagcgca tgaagaacgg
     1021 ccaggaagtg cagccgtttt tcaataactc acgtgactac ataagcgcac aggaagtaac
     1081 cttcaaaata gacaacaaca aggcaaaatt aagccggaac gatgcgaagt tctatgtgat
     1141 cacggtaagc ccttccagcc gggagctgga gaaaatgggc aagacagaga aggaacaggc
     1201 tgaagccatg agaaagtatg tccgtgatga tgtgatgcag cactatgcgg aagggttcgg
     1261 aaaaggtctg aacaaagagg atatcgagta ttacggaaaa atccatttcg agaggaaggg
     1321 agccgaccgg tacgacatgc acgcacatat cattgtcagc cggaaggaca ggagcaacac
     1381 caggaagcta agcccaaaga ccaaccacac cgggaagaag aactgcggaa acgtgaaggg
     1441 cggttttgac agaacagatt ttttccgaaa gtgtgaaacc tcatttgata agcgcacagg
     1501 gtatgaccgg gctccggaac agacattcga ctatctcaat accatgaaga acggaagccc
     1561 gaaagaaatc ttccagaaaa aggaatgggc ggaacgtgtg aaccatgaac ggcttgagaa
     1621 gatgaaggcc gaatggagcc gggatcttca ggaactgcac caggaacaag gccgtgaaga
     1681 gagccaacaa cagggaaaca gtatatccca agttccggat atcaaccagg caccgcagag
     1741 aaagaaacaa caggaggaag aaatcgacca gcccaggaaa agaagccggg gttttggaat
     1801 gggtatgtga ttttctgatt gtgcaataaa gatatgggca agttcaagaa tgcttttagg
     1861 gtatatggct ggaatacaac tacaatcatt tctacattac cattcgcata taaatgggaa
     1921 ggataattta cctgaccact aatagtaaat tctgattgag cagttttcaa ttctgggatg
     1981 taaagcggtg tttgtttgtg gaaaatgatt tgaggacagc caatcggaaa agtaagggta
     2041 ttcagcggtt gattgctttt gaatacccaa taatacctga cgtaaggttg taactccttg
     2101 caagacttgt aaaatttgaa gccctcttga atcattttac aaaggtaatg ctttttgtcg
     2161 tttcttattg attacgagca aaaatatagc tgcaaatgaa ataacttgca gccatatttt
     2221 tatcggtaca tattagtcgg tataaagtct tatgcggtta cccgcagggt tagtgagtac
     2281 agaaaaatac tcttttcctt aaatctctcg acgtactcat ctaaatcgtt ttttcaagag
     2341 gttttctctg aagtacaaaa cttttctttc tctctaacca aatcgatggc ctccttgccc
     2401 gtcatgtgtt cgatatcgat ctttaccatc agtagatggt tgatgctttt ggctatttca
     2461 tcagacaaac ctggctcacc gaaagaatat ttattcacca gcaaagtcat agccatacgt
     2521 ttttctgttt cgtcggttaa aatataggcc tttccaaaga ctatcacact tcgaaagtag
     2581 gtcgtgaatt cggcaggttt aatattgtct tgttccacaa cgcaaaacga tactttattg
     2641 ttttgcataa tagcgtccac cttatgccct atcttggcgc aatgaaaata gattttgcca
     2701 tcagcataaa aataactgag aggaacggca taaggatagc cattatctcc gtggagagct
     2761 aaagtcccgt ttgtcatttt ttcaagaatt gccacgctct cttcttgtgg caacaactgt
     2821 cgtttacgac gcatttctct gaacatatta ctgatcttta gtattaaaca atgaagtgat
     2881 ttaaacttat ttcattctct taaaatcgta tcaaaatgtt aaagcacagc gtacaaattg
     2941 aattgttccc tatattatgg tgaccaaact catatatagt gaaagtccaa tgtacactat
     3001 gctcgacaaa gatacgataa aaaatgaaat attgcctcat ctttccaagg caaaacgtgg
     3061 ttatgccact caaagttgtc tcatagaggt ggtaaacgcc atcttgtaca agttgaagac
     3121 cggttgtcaa tgggcgttcc ttcctgtgga agccctgttt agcggcaagg tactgagcta
     3181 tggtgccgtg tatcatcatt acaacaaatg gagcaagtcg ggcgagtgga aatccatgtg
     3241 ggtcaggttg cttgacaaac accgccataa gttggacatg tccagcgtgg atttggacgg
     3301 gagccacacg gctgcactcc gtggcggtga agaagtcgga taccagggac ggaagaaacg
     3361 gaagacgacc aacgcactct acctgacaga caggcaagga cttccgattg ccatgtcttc
     3421 tccgaaatct ggcgaacacc atgacgtaca tgacatagaa aaggtcatcg gtgacatgtt
     3481 caacgacctt gagaaatccc atatccgggt tgatggtctt ttcctcaatg ccgatgccgg
     3541 attcgattgt gatgtcttac gtggtttcct gggtgaaaaa gagatcatcg caaacatctg
     3601 catcaataaa cggagagaaa atgccgatga catctttgtt gatgatgaac tatatgctga
     3661 aaggtattcc atcgaacgaa ccaacgcttg gatggacagt ttcaggacgc tactgaaccg
     3721 gttcgataca actgtttcaa gttggatgtc ttggaattac ttggcatttg cagtcttgtt
     3781 attgaagaaa atttcacgaa aacaaaaagt ttaaatcact tctttattct gagagtatgg
     3841 acttagtgct gttcacaaag aatttttgcc gttcacaaaa caaatttgca gatatccgga
     3901 aaaagttaca ggagccagtt gctcaaaact gtcccggtat tgtgccgtta taattgcaga
     3961 agaaaacgga attgcttata tttgtagaac tcaagcgtag gctctggcaa gcccattgcg
     4021 gaaatgcatg ggacatggcg gctggtttcg gtcgccattt ttatttgcca aaaaaaaggg
     4081 gcactcttgc ccccatgtat ttcctcatat cttcacagac tgaaatacta atacaacaat
     4141 aaaataaaag ctaaaagggg ttatcagtcc tcaccgactg agggaaagag gaaaaagact
     4201 tgccctaata gaatttattt atttattaac atatgtccta tgggaatgta ctccatagtt
     4261 agccacaaag ttataaaaaa gagccaaagt tccaatcgtt taccatgccg tttttctacc
     4321 tattttctac ccgactggct gttacagttc tgtatttact tttattttcc ctatcgtttt
     4381 ctgttcgtca cgcacccggc aaagttcccg gtgtgctttt tccttatatt cctgaagaat
     4441 tttgatttcg ttacgtaatc ggttgttctc ttcctgaagg agctgaaggt ctttggcacg
     4501 ttctgtagcc agactttctg ccttcgcctt tgcttctgtt gcttggacta taagttccgg
     4561 agccaccgca ccaggaacaa ggccgtgaag agagccaaca acagggaaac agtatatccc
     4621 aagttccgga aatcaatcag gcaccgcagc ggaagaagca acaggaggaa gaactcgacc
     4681 agcccaggga aagaagccgg ggttttggaa tgggtatgta attttatagc tgagagtatg
     4741 gttctttgtg tcaataagaa taactctata aagaatcttt gtacatatca agacatattt
     4801 tcatgagctt taatgaagac tctaattggg aatatccttt atcaattgtc atataacttg
     4861 acaatagcag tttcttctca acctcagtat aaggatatgt aataatttta agccagaaat
     4921 aagttttctt tgtttcaatt aaatcttgat atgatagtag tttgtctttg gttgcttcgt
     4981 aattgtcttt gtatgctttg tataatttat aatcaaaatt gtgtgtatag atatttattt
     5041 gtattgcatt agttctgaca cctatatcgt tawtaaatat agacaaatcg ttgttaagtg
     5101 tatataattc atacgataat tttatatatg ataattcatg ctcaagagaa tcaactcttt
     5161 gagttaggct tatgtccttt gtgctatcag caggagcaac cataaatgac gaatcattct
     5221 tttgtgcata tgcgatagtt gtgcataata gtactaaaat cagtaccaat tcctttctaa
     5281 attcgttctt tttcataatt gatattttat aactctgtat ttacttttat tttcccgaaa
     5341 gttttctgtt catcacgtac ccggcaaagt tctcggtgag ccttttcctt atactcctga
     5401 agggctttga tttcgttacg caatcggtcg ttttcttcct gaagaacctg gagctcttta
     5461 acctgttctt cagacttcat ttcagcacgt accttttctt ctgctgcctt tgcgataagt
     5521 tctggagcag gaagctgttt tgcggtctgt tcccggactg cctgtagtag ttggcgaatc
     5581 gcttctgttc cttcattgct tgtctgcatg gtcgctttta cttcattcaa ttctccgatg
     5641 atcttttcca agggggagta atcaagtgcg ttactcttca ggtcgaaacc tgtgcgttca
     5701 aagtattcca gggcgagaga aacgaactct ttccgtaata tgccgtttgc ccggcagtat
     5761 atctccagcc gttcagatgt ttctccgctt attgagattg tagtcttttc cggcttactt
     5821 ttgggttgta tggttacttt atctgtcata ccttaaccga ttgatataaa tatgcttata
     5881 atatgcttga tatgctgatt ttatacttct ttttaattga atatgctgta aatatactga
     5941 atattcctga aatatattca ggttacttgg ccttttcttc gcttaaagtg atatgagaat
     6001 ccacaaagca ccctccgaag ccggataagg catcaggttg cgtgtaagtg accgttacaa
     6061 gggtttgtcc catgaatgtt tcaatcttgt ctataacggc ttccattggc ttgcctaagc
     6121 ctgtggcttc agcagatacc aaaatcaaat ctccttcttt catatcgttg ctttttgatt
     6181 ttgttacaga ttcaaagttt ctttctatct ttaatttttc ccttgatggc attgattata
     6241 taacctttgg ggttcttctt ttcccttgat ttccctttta ggatggcaag ctcgttaagt
     6301 aaatccggga gtaaggtttg tgctgatata aacaaatcca ggttgttctt aatctcttta
     6361 tctgaaaatt caatcgagtt tttaagatag cttcttactt caggagccaa agaccagctt
     6421 aaagctgtct gtttttgaag gtcgtgtttt tccagatcgc tatcacgatg ttctggttga
     6481 tataccggat aaaactttat ggccgttatc tttcgtcctg ttttgagcgg tgtgtactca
     6541 aaggtataag ggctgacttc atcaagctct ttttttgcag cttctactac tttgcgcatg
     6601 aaatcattta ttcgctcata cttatctgcc acacaaaaca tttcctttag ctgttctata
     6661 gagtatatca gaggagtttt ctggttgctc agcaactcat aaaagcgcat tgaaaactgg
     6721 ctcttaaagc tcatggcagc tttcaattcg tattttctga agcctttgga gaaatcaagt
     6781 atgcagtccc atacacgtgg gtgcacctga aaggacaaga ctgaagaacg ttttcgtata
     6841 taaggcaagg ctatgataga tatactttgc cagatttcat catcttcata ctcaaagatc
     6901 tttcgctgaa gcgactgtag ggcttctttt actcttgcat aatttttgtc gtcctctcca
     6961 ttaagcaggc aggcaatagg catggttata tttacgcaac tgaaaagatc atgctctatc
     7021 ttctgacagt tatcagcgaa ctttatacct tgcagctctc cttgagccat ttctacaaga
     7081 cgataaatga tacgcttttc gtacacgtta aagtcatatt ttgcagtagt cataatataa
     7141 ctttggataa ggtctttgtt tactattgcc ataattatca gtgttttgtg caaatataag
     7201 tattaaaaac gactttaaca aataaaagtc gtagattgtg tgattagtat tcaaaaagtc
     7261 gtagttaaac tttgaaatag tattcgaaaa gtcgtagtta gtattcaaaa agtcgtagtt
     7321 ggattcttgt tgttgcttgt aatttactgt ttatcagatt gttaaagcat gtttttcgga
     7381 agtcgataag catataacaa gtaatgctta aaaacaagtc ataaaatttt agccggaatg
     7441 tggcttccag accttcaaat ccgtgaaaaa cgactttttg aatgtttgtg aaagcaaagc
     7501 aaaggccagc gcagcgttct ttgaggaacg aactttccgc gcgtgtacac aaataagtac
     7561 acaaaacttt tgttttattg gtttttatcc ctatttttgc agtgtaaacc aatatattag
     7621 aactatgcag attgttacaa cacgcgaatt tcgtgccaac cagaagaaat acttcgaaat
     7681 ggcagagacg gaaacgatcc tggtctccag acgcaatgca gcccctatta tggtgtgtgc
     7741 agtcagagaa ggtgatttcc cttcaaagga agaacttgcg gccatccaga gaggtattga
     7801 agatatccga aacggaaata ccttcagaat ggctaaaaac gagtctctgg atgattttct
     7861 taaccgtata gagggcgaag gcaatgtatg agatagaatt ttccaaagaa gcggtaaaac
     7921 atgtcctgtt gatgagaaaa tcctctccgc agcttttcag gaagctggag aagctgcttg
     7981 atgaactccg ggaacacccg tacacgggta cagggcatcc ggaacagttg agatacctgg
     8041 agggtgtctg gtcaaggcag ctggacaaga agaaccgtat caggtatatg gtcaacgaga
     8101 ctaccgtaat tgtgtttatc atttcagcct gggggcatta tgacgacaaa tgaaggattg
     8161 cagtaagttc cgaaacggca catactgcaa tccttttgaa tttgtgacag aatcaaaata
     8221 acttatatca tatatccaag caaacggctg cactcgtcgg gagtgttggt acaccccctt
     8281 gccgtgcggc gctccccgac cgatgagttt ttgtggtgtt ccggacacgt t
//
