LOCUS       NZ_MW364977             4439 bp    DNA     circular CON 29-APR-2024
DEFINITION  Staphylococcus epidermidis strain SE27 plasmid pSE27_T, complete
            sequence.
ACCESSION   NZ_MW364977
VERSION     NZ_MW364977.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Fisarova,L., Botka,T., Du,X., Maslanova,I., Bardy,P., Pantucek,R.,
            Benesik,M., Roudnicky,P., Winstel,V., Larsen,J., Rosenstein,R.,
            Peschel,A. and Doskar,J.
  TITLE     Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance
            Plasmids and Mobilize Chromosomal Islands
  JOURNAL   mSphere 6 (3), e00223-21 (2021)
   PUBMED   33980677
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Botka,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2020) Department of Experimental Biology, Faculty
            of Science, Masaryk University, Kotlarska 2, Brno CZ 611 37, Czech
            Republic
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_017354795.1-RS_2024_04_29
            Annotation Date              :: 04/29/2024 03:26:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW364977.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="SE27"
                     /db_xref="taxon:1282"
                     /plasmid="pSE27_T"
     gene            44..1423
                     /gene="tet(K)"
                     /locus_tag="J4G94_RS00005"
     ARG             45..1420
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1609..2850
                     /gene="mobV"
                     /locus_tag="J4G94_RS00010"
     CDS             1609..2850
                     /gene="mobV"
                     /locus_tag="J4G94_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            3377..4321
                     /locus_tag="J4G94_RS00015"
     CDS             3377..4321
                     /locus_tag="J4G94_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          3378..4321
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1610..2847
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaccccta taaactttag tcacctcaag taaagaggta aaattgttta gtttatataa
       61 aaaatttaaa ggtttgtttt atagcgtttt attttggctt tgtattcttt cattttttag
      121 tgtattaaat gaaatggttt taaatgtttc tttacctgat attgcaaatc attttaatac
      181 tactcctgga attacaaact gggtaaacac tgcatatatg ttaacttttt cgataggaac
      241 agcagtatat ggaaaattat ctgattatat aaatataaaa aaattgttaa ttattggtat
      301 tagtttgagc tgtcttggtt cattgattgc ttttattggt cacaatcact tttttatttt
      361 gatttttggt aggttagtac aaggagtagg atctgctgca ttcccttcac tgattatggt
      421 ggttgtagct agaaatatta caagaaaaaa acaaggcaaa gcctttggtt ttataggatc
      481 aattgtagct ttaggtgaag ggttaggtcc ttcaataggg ggaataatag cacattatat
      541 tcattggtct tacctactta tacttcctat gattacaata gtaactatac cttttcttat
      601 taaagtaatg gtacctggta aatcaacaaa aaatacatta gatatcgtag gtattgtttt
      661 aatgtctata agtattatat gttttatgtt atttacgaca aattataatt ggactttttt
      721 aatactcttc acaatctttt ttgtgatttt tattaaacat atttcaagag tttctaaccc
      781 ttttattaat cctaaactag ggaaaaacat tccgtttatg cttggtttgt tttctggtgg
      841 gctaatattt tctatagtag ctggttttat atcaatggtg ccttatatga tgaaaactat
      901 ttatcatgta aatgtagcga caataggtaa tagtgttatt tttcctggaa ccatgagtgt
      961 tattgttttt ggttattttg gtggtttttt agtggataga aaaggatcat tatttgtttt
     1021 tattttagga tcattgtcta tctctataag ttttttaact attgcatttt ttgttgagtt
     1081 tagtatgtgg ttgactactt ttatgtttat atttgttatg ggcggattat cttttactaa
     1141 aacagttata tcaaaaatag tatcaagtag tctttctgaa gaagaagttg cttctggaat
     1201 gagtttgcta aatttcacaa gttttttatc agagggaaca ggtatagcaa ttgtaggagg
     1261 tttattgtca ctacaattga ttaatcgtaa actagttctg gaatttataa attattcttc
     1321 tggagtgtat agtaatattc ttgtagccat ggctatcctt attattttat gttgtctttt
     1381 gacgattatt gtatttaaac gttctgaaaa gcagtttgaa tagttatatt atattttggt
     1441 ttagaactat gagtggctag cattttgcca ctcatttttt gcgttagcaa aaacaggttt
     1501 aagcctcgca gagcacacgt attaacgact tattaaaaat aagtctagtg tgttagactt
     1561 aaactattaa atacacatga aacctttgtg cttaggagtg atttttatat gtcttattcc
     1621 attgttagag tttcaaaagt taaatctgga acaaatacaa cgggcataca aaaacatgtt
     1681 caaagagaaa ataataatta tgaaaatgaa gatatagacc atagtaaaac ttacttaaat
     1741 tatgatttgg taaatgctaa taaacagaat tttaataact tgattgatga aaaaatcgaa
     1801 cagaattata caggcaaaag aaaaattaga acagacgcga ttaaacacat tgatggttta
     1861 attacatcag acaatgattt ctttgataat caaacgccag aagatacaaa gcagtttttt
     1921 gaatatgcta aagagttttt agaacaagaa tacggtaaag ataatttatt atatgcaaca
     1981 gttcacatgg acgaaaaaac accacatatg cattatggcg ttgttccaat aactgatgat
     2041 ggtcgtttaa gtgctaaaga agttgtaggt aataaaaaag ctttaacagc gtttcaagat
     2101 agatttaatg agcatgttaa acaacgagga tatgatttag aacgtgggca atcaagacaa
     2161 gtaacaaatg ctaaacatga gcaaataagt cagtataaac aaaaaacaga atatcataag
     2221 caagaatatg aacgtgagag ccaaaaaaca gaccatataa agcaaaagaa cgataaatta
     2281 atgcaagagt accaaaaatc gttaaatacg cttaaaaagc ctataaatgt tccgtatgag
     2341 caagaaactg aaaaagtagg tggtttattt agcaaagaaa tacaagaaac tggaaatgtt
     2401 gtaataagcc aaaaagattt caatgaattt cagaaacaga taaaagctgc tcaagatatt
     2461 tcggaagatt acgagtatat aaagtctggt agagccttag atgataaaga taaggaaata
     2521 cgagagaaag atgatttatt aaataaagca gttgagcgta ttgaaaacgc agacgataat
     2581 tttaaccaac tttacgaaaa tgcaaagcca cttaaagaga atatagaaat agcgttaaag
     2641 cttttaaaaa tcttactaaa agagttagaa cgagttttag gaagaaatac ctttgcggaa
     2701 agagttaata agttaacaga agatgaacca aaactaaatg gtttagcagg aaacttagat
     2761 aaaaaaatga atccagaatt atattcagaa caggaacagc aacaagaaca acaaaagaat
     2821 caaaaacgag atagaggtat gcacttatag aacatgcatt tatgccgaga aaacttattg
     2881 gttggaatgg gctatgtgtt agctaacttg ttagcgagtt ggttggactt gaattgggat
     2941 taatcccaag aaagtaccaa ctcaacaaca cataaagccc tgtaggttcc gaccaataag
     3001 gaaattggaa taaagcaata aaaggagttg aagaaatgaa attcagagaa gcctttgaga
     3061 attttataac aagtaagtat gtacttggtg ttttagtagt tttaactgtt taccagataa
     3121 tacaaatgct taaataaaaa aagacttgat ctgattagac caagtctttt gatagtgtta
     3181 tattaataac aaaataaaaa ggagtcgctc acgccctgac caaagtttgt gaacgacatc
     3241 attcaaagaa aaaaacactg agttgttttt ataatcttgt atatttagat attaaacgat
     3301 atttaaatat acatcaagat atatatttgg gtgagcgatt ccttaaacga aattgagatt
     3361 aaggagtcga ttttttatgt ataaaaacaa tcatgcaaat cattcaaatc atttggaaaa
     3421 tcacgattta gacaattttt ctaaaaccgg ctactctaat agccggttgg acgcacatac
     3481 tgtgtgcata tctgatccaa aattaagttt tgatgcaatg acgatcgttg gaaatctcaa
     3541 ccgagacaac gctcaagccc tttctaaatt tatgagtgta gagccccaaa taagactttg
     3601 ggatattctt caaacaaagt ttaaagctaa agcacttcaa gaaaaagttt atattgaata
     3661 tgacaaagtg aaagcagata gttgggatag acgtaatatg cgtattgaat ttaatccaaa
     3721 caaacttaca cgagatgaaa tgatttggtt aaaacaaaat ataataagct acatggaaga
     3781 tgacggtttt acaagattag atttagcctt tgattttgaa gatgatttga gtgactacta
     3841 tgcaatgtct gataaagcag ttaagaaaac tattttttat ggtcgtaatg gtaagccaga
     3901 aacaaaatat tttggcgtga gagatagtaa tagatttatt agaatttata ataaaaagca
     3961 agaacgtaaa gataatgcag atgctgaagt tatgtctgaa catttatggc gtgtagaaat
     4021 cgaacttaaa agagatatgg tggattactg gaatgattgc tttagtgatt tacatatctt
     4081 gcaaccagat tggaaaacta tccaacgcac tgcggataga gcaatagttt ttatgttatt
     4141 gagtgatgaa gaagaatggg gaaagcttca cagaaattct agaacaaaat ataagaattt
     4201 gataaaagaa atttcgccag tcgatttaac ggacttaatg aaatcgactt taaaagcgaa
     4261 cgaaaaacaa ttgcaaaaac aaatcgattt ttggcaacat gaatttaaat tttggaaata
     4321 gtgtacatat taatattact gaacaaaaat gatatattta aactattcta atttaggagg
     4381 atttttttat gaagtgtcta tttaaaaatt tggggaattt atatgaggtg aaagaataa
//
