LOCUS       NZ_MW364976             4397 bp    DNA     circular CON 29-APR-2024
DEFINITION  Staphylococcus epidermidis strain SE27 plasmid pSE27_S, complete
            sequence.
ACCESSION   NZ_MW364976
VERSION     NZ_MW364976.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Fisarova,L., Botka,T., Du,X., Maslanova,I., Bardy,P., Pantucek,R.,
            Benesik,M., Roudnicky,P., Winstel,V., Larsen,J., Rosenstein,R.,
            Peschel,A. and Doskar,J.
  TITLE     Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance
            Plasmids and Mobilize Chromosomal Islands
  JOURNAL   mSphere 6 (3), e00223-21 (2021)
   PUBMED   33980677
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Botka,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2020) Department of Experimental Biology, Faculty
            of Science, Masaryk University, Kotlarska 2, Brno CZ 611 37, Czech
            Republic
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_017354785.1-RS_2024_04_29
            Annotation Date              :: 04/29/2024 03:26:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW364976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="SE27"
                     /db_xref="taxon:1282"
                     /plasmid="pSE27_S"
     gene            complement(5..853)
                     /gene="str"
                     /locus_tag="J4G90_RS00005"
     ARG             complement(9..853)
                     /name="str"
                     /gene="str"
                     /gene_name="streptomycin adenylyltransferase Str"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(861..1805)
                     /locus_tag="J4G90_RS00010"
     CDS             complement(861..1805)
                     /locus_tag="J4G90_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167206.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_011167206.1"
                     /translation="MSKKAEEIQAKQSLEKENSNFSKTGYSNSRLNRHIMYTPEPKLHF
                     DAMTIVGNLNKNNAHKLSEFMSIAPQIRLWDILQTKFKAKALQEKVYIEYDKVKADAWD
                     RRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKSVK
                     KTIFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADIEVMSEHLWRVEIELKRDMV
                     DYWNDCFNDLHILKPDWSSLEKVKDQAMIYMLIHEESTWGKLERRTKNKYREMLKSISE
                     IDLTDLMKLTLKENEKQLQKQIEFWQREFRFWE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(2054..2245)
                     /locus_tag="J4G90_RS00015"
     CDS             complement(2054..2245)
                     /locus_tag="J4G90_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_014532527.1"
                     /translation="MGLLRVQRLNVNKAIGIKQQRGLKKMSKKKKINLAISIAQVVVQA
                     LILYNTYQQYKYSKKYSA"
                     /name="hypothetical protein"
     gene            complement(2366..2821)
                     /locus_tag="J4G90_RS00020"
     CDS             complement(2366..2821)
                     /locus_tag="J4G90_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="WP_000753824.1"
                     /translation="MKKTTEDFQGRGKKVHNDFVRLLQKNLNRVNAEDIEIEVRKDVYG
                     VREEIHSMLREVKQSHEHYQKRQKQLFTGIGAMLLVFMLFALIMTIGRDFMGFLHVDVL
                     QNAIASKIKASEGFISVLWYIAYGLPYILAIGLFIWLYEWIRARFHD"
                     /name="DUF334 domain-containing protein"
     gene            complement(2790..3752)
                     /locus_tag="J4G90_RS00025"
     CDS             complement(2790..3752)
                     /locus_tag="J4G90_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000246202.1"
                     /translation="MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRA
                     LYGKENGVQAHTVIQSFKPGEVTAKECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHI
                     IINSVNLETGNKYQSNKEQRDFIKKANDQLCEERGLSVPEKSSEIRYTLAEQNMIDKDK
                     RSWKNDIRMAVEETKDNAVDFEEFNTLLKEKGVEITRVTKNNVTYRHIEEDKKVRGNKL
                     GDSYDKGVIENGFAIEKFRREREEEREYDEYADTFEVDWDAVAENSEDLRKRRIARTEE
                     TKQASNKIYIRDERTTGLERKGIAGNQVEFEKDDGGLSR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3734..4132)
                     /gene="mobC"
                     /locus_tag="J4G90_RS00030"
     CDS             complement(3734..4132)
                     /gene="mobC"
                     /locus_tag="J4G90_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_001002114.1"
                     /translation="MNEHKDNLASDLSVGGNRKPNRKEPKQISFRVSEQEYSKLKQSAE
                     TLNMTVPNFVKKKAQGSRLVSPKLDKETRQSIAKDLSHLGSNVNQIAKWFNQNKDKATH
                     LPEEKYHDLEKQFDDVKKELHKIWQQLN"
                     /name="mobC"
     Typing          4104..4338
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="100.0"
                     /evalue="1.4099999999999999e-40"
     Typing          complement(862..1805)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2794..3752)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaattaatt ctcataatta ttacgaataa aattctcaat atttttttcg taatttggat
       61 agttataatt tcctaaacaa cttactttat ttgaataata tttaaataat tcacaacaaa
      121 gcttaaaaga ttggtatgtc tttctatatc cattcatctc aaaagtagct aaaagcatat
      181 tgaattcttt atcagttaaa tatttgttta taaacttata attctttcct aaactaaaat
      241 caaaacccct attaaagcca atataccaag aaatcattct taataattca ggacgtaaaa
      301 tattattgaa atgatctaaa gcaaataata tttctcttct aaaaacaccc tttgctacat
      361 acgttgagac actccaaaac tcattacagc aatcataaaa ttctcgttct gttggttttt
      421 ttaaccaata atttgagtca tctggaacaa tttcttgagt tactaaatta tctttatcaa
      481 ctaaaatttt tacaagacca tcagaatcac taaaataacg atttaaatct tttaaattaa
      541 ttaatgtaat atccattttt atgccatctt taaaatacat tatataactg taaccataat
      601 ctaaatcagg tgggaataat tccatatctt ctggtttctg tataaacaat aattctccaa
      661 ataaacttaa ccaactttct tcatgtgtga aatactcaat atctgatacg aaaaaagcaa
      721 aatcataatc ttgaaactta tcttttttta tattttcatt agttcttgaa ccctcgagag
      781 caataatttt tacattgctt cgttgataag caaattcaga aactaaattt aaaatttctt
      841 tttcagtcct cattgttttt tcactcccaa aatctaaatt cacgctgcca aaattcaatc
      901 tgcttttgca attgtttttc attctctttt aaagtcaatt tcattaagtc cgttaaatca
      961 atttctgaaa tactttttaa catttctctg tatttatttt ttgtacgtct ttctaattta
     1021 ccccatgtac tttcttcatg aattagcata taaatcattg cttggtcttt tactttttct
     1081 aaactagacc aatctggttt caaaatatgt aaatcattaa aacaatcgtt ccaataatca
     1141 accatatctc tttttaattc aatttctaca cgccataagt gttcagacat aacttcaatg
     1201 tctgcattat ccttacgttc ttgtttttta ttataaattc taataaatct atcactgtca
     1261 cgaacaccaa aatattttgt ttctggttta ccgttacgac cataaaaaat agttttctta
     1321 actgatttat cagtcatcgc ataataatcg ctcaaatcgt cttcaaaatc aaaagccaaa
     1381 tctaatcttg taaaaccatc atcttccatg tagtcaatga tgttttgttt taaccaaagc
     1441 atttcttcat gcgtaagttt attaggatta aattcaacac gcatattacg tctatcccac
     1501 gcatctgctt ttactttatc atattcgatg taaacttttt cttgtagagc cttagcttta
     1561 aatttggttt gtaatatatc ccaaagtcta atttgtggcg caatactcat aaattcagac
     1621 agtttgtgag cattgttctt attaagattc ccaacaattg tcatagcatc aaagtgtaat
     1681 tttggttcgg gggtgtacat aatatgtcgg tttaaccggc tattagagta tccggtttta
     1741 gaaaaatttg agttttcctt ttccaaactt tgttttgcct gaatttcttc tgctttttta
     1801 ctcataaaaa atcgactcct tattttgttt aagaagtcgc tcacccgaat atatatcttg
     1861 aagaataaac caatatcgtt taatatctag atatacaaaa attaagaaaa aaacaccaac
     1921 tgtttttctt taaggtaagt cgcattcaca aactttggtc agggagtgag cgacttcttt
     1981 ttttgttttt ttatttgcat attaatataa cactaacaaa aggcttggtc tattcagact
     2041 aagtcttttt ttattaggct gaatattttt tagaatattt atattgctga taagtgttat
     2101 aaagtattaa cgcctgcact actacttgag caattgatat tgctaaatta atctttttct
     2161 tcttactcat tttcttcaac cccctttgtt gctttattcc aattgcttta tttacattga
     2221 gcctctgtac ccttaacaaa cccaaaactt gtcgaatggt cgggctaata gctcacgcta
     2281 tgccgacatt cgtctgcaag tttagttaag ggtacttctc aatataaata aattttctcg
     2341 gcataaaagc ctgatcatac tttgtttaat catggaatct tgctcttatc cactcataca
     2401 accaaataaa tagccctata gcgagtatat aaggtaaacc ataagcaatg taccataaaa
     2461 ctgaaataaa gccctcagaa gcctttattt tgcttgctat ggcattctgt aatacatcta
     2521 catgtaaaaa gcccataaaa tctctgccaa tcgtcataat caaagcaaaa agcataaaca
     2581 ctaataacat tgcaccaata ccagtaaata attgcttttg tcttttttga taatgttcat
     2641 gtgattgttt aacttcacgc aacatcgaat gaatttcttc tcgaactcca taaacatctt
     2701 ttcgaacctc tatctcaata tcttctgcat tcactcgatt taaattcttt tgtaacaaac
     2761 gaacaaaatc attatggact tttttacctc taccttgaaa gtcctccgtc gtctttttca
     2821 aactcgactt gattgcctgc aattcctttt ctctcaagtc ctgttgttct ttcatctctg
     2881 atatatattt tattactagc ttgttttgtt tcttcagttc ttgcaattct tcgttttcta
     2941 aggtcttctg aattttctgc gactgcgtcc cagtcaactt cgaacgtgtc tgcatattca
     3001 tcgtattctc gttcttcttc tcgttctcgt ctgaatttct cgattgcaaa gccattttca
     3061 atcactcctt tgtcataaga atcgcccaac ttattcccac gtactttttt atcttcttca
     3121 atgtgtctat acgttacgtt gtttttcgtt actctcgtga tttcaacacc tttttctttt
     3181 agcaaagtat taaattcttc aaagtctact gcgttatcct ttgtttcttc aactgccatt
     3241 cttatatcat ttttccatga ccttttatct ttatctatca tgttctgttc tgccaacgta
     3301 tatctgattt ctgatgactt ttctggcaca gataaaccac gttcttcaca taactgatca
     3361 ttcgcctttt ttataaaatc acgttgttct ttgtttgact gatacttgtt gcctgtttct
     3421 aaatttactg aattaataat aatgtgattg tgataatggt ctttgtctgt atgtgtataa
     3481 actgcaactt gataatctgg tgcaattttt ttagctaatt ctaaacctat ctcattacac
     3541 tctttagctg ttacttcgcc aggcttaaaa gattgaatga ctgtatgtgc ttgaacacca
     3601 ttctctttgc catataacgc cctagtttgc ttaaaatatg atttagcata atcaacatca
     3661 caatttaagc cgcttttttc ttctgcacgt tcttctgcgt aattaatggc acgacttgcc
     3721 gacttcgtat tgcctaattt agttgttgcc atatcttatg caactccttt ttcacatcat
     3781 caaattgctt ttctaaatca tgatatttct cttctggtaa atgtgtagcc ttatctttat
     3841 tttgattaaa ccacttagca atctgattca cattactacc caaatgactt aaatctttgg
     3901 caatcgattg tcgcgtttct ttgtctaatt ttggcgacac taatcgactg ccttgtgcct
     3961 ttttcttaac gaaattaggc actgtcatat tcaaagtttc tgccgactgt ttcaactttg
     4021 aatattcctg ttcactcact ctgaaactaa tttgttttgg ctctttgcgg ttgggtttac
     4081 ggtttccccc aacagacaaa tcgctagcca aattatcttt atgttcgttc acaagtccca
     4141 ccacctttct gacgaaaaat attccttttc ataaatatag cttataatat aatctatatt
     4201 ttctcaagta atatagtggt ttggcaagcg tcgtatattc tagccacttc gttctgaata
     4261 taccggcttg ctgggattaa tcccagaccc tttatgtttt tgctaacgca aaaaatgagt
     4321 ggcaaaatgc tagccactca tagttcaaaa ccaaattcaa aatcaaaaca aaagcaacca
     4381 aaaatgtggt tgctaat
//
