LOCUS       NZ_MW240712             3173 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Escherichia coli Nissle 1917 plasmid pMUT1, complete sequence.
ACCESSION   NZ_MW240712
VERSION     NZ_MW240712.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli Nissle 1917
  ORGANISM  Escherichia coli Nissle 1917
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Kan,A., Gelfat,I., Emani,S., Praveschotinunt,P. and Joshi,N.S.
  TITLE     Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E.
            coli Nissle 1917
  JOURNAL   ACS Synth Biol 10 (1), 94-106 (2021)
   PUBMED   33301298
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Kan,A., Gelfat,I. and Joshi,N.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2020) Wyss Institute for Biologically Inspired
            Engineering, Harvard University, 3 Blackfan Circle, Boston, MA
            02115, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology        :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/08/2024 02:08:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW240712.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Escherichia coli Nissle 1917"
                     /mol_type="genomic DNA"
                     /strain="Nissle 1917"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:316435"
                     /plasmid="pMUT1"
                     /geo_loc_name="Germany: Freiburg"
                     /collection_date="1917"
     gene            complement(207..635)
                     /locus_tag="JNG15_RS00005"
                     /old_locus_tag="ECOLIN_RS25935"
     CDS             complement(207..635)
                     /locus_tag="JNG15_RS00005"
                     /old_locus_tag="ECOLIN_RS25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            927..1355
                     /locus_tag="JNG15_RS00010"
                     /old_locus_tag="ECOLIN_RS25940"
     CDS             927..1355
                     /locus_tag="JNG15_RS00010"
                     /old_locus_tag="ECOLIN_RS25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(2190..2384)
                     /locus_tag="JNG15_RS00015"
                     /old_locus_tag="ECOLIN_RS25945"
     CDS             complement(2190..2384)
                     /locus_tag="JNG15_RS00015"
                     /old_locus_tag="ECOLIN_RS25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2668..2991
                     /gene="mobA"
                     /locus_tag="JNG15_RS00020"
                     /old_locus_tag="ECOLIN_RS25950"
     CDS             2668..2991
                     /gene="mobA"
                     /locus_tag="JNG15_RS00020"
                     /old_locus_tag="ECOLIN_RS25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          2423..2659
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          complement(1631..2155)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.085"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2669..2988
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.23e-62"
ORIGIN
        1 gtttcagtgg tgcgtacaat taagggatta tggtaaatat atgagctatg cgataacttt
       61 aactgtgaag cgatgaaccc attacaggca aagccaatta ctcctgacag tggtttagcc
      121 agaagcaggg ctaccaagac caatgcaata agtaatatat cgttttgcta tcgtgcatcg
      181 ctcgcgctca gttccattgt gcttttttaa gctgtcgttt ttcttacggt atataccggt
      241 tttttatggc gtggtttctt aacttgttca gctactgatg gacccattgt atctaggtag
      301 tcaactagct ttgttagatc ataaatattg cgaccaccat atcggcgatc actcttcgat
      361 gttggtttct tgtccaagag attagctttt tcaagatcat tgatagctct ctgaacagtc
      421 cgtacagaaa cccccatacg tatggctaga ctttccattg acggatgcgg ccactcttgc
      481 aaactccacc agtgaacgat caggttaagt agtgtgttaa aggccactga agttagcttt
      541 ttctcgtttt gtataaaaaa caatacggta ggcactgctg tccagccaag agacaaaccg
      601 ccagctttcc atttattctt aacggagtaa gtcattgatt ttcctaagcc ccaaaatatt
      661 taaagtatat attatatggt atattcatat gaatagggtg acactggcgc cattattgtg
      721 caaccaaaaa agactactct gaaaacgagg aaaagatttt ttcctgcctg aattagatac
      781 ggagttagcg atatgaaaac cgaacaacgt catgatcttg ttaaagatat tgaggttttt
      841 ggcgtatcct tgtctctgtt gatttccaga gcgaatgaga agtctgttac aatgccatct
      901 ggtctaagtc gggagcagag aagagcatgg gcagcggagc aggcgcgcaa aatccacaat
      961 tgaatattgt ctcattctct gagaccttca acctttatta cacatccaga tattctgcaa
     1021 aaacactcga taaaatcgat gatttcattg agcattttga aaaatacaat ctctttggcg
     1081 atcctttaaa aggatatcca gcttggactg gcaaagtatc gccatcgtgg aaagtgcctg
     1141 atcattacga aaacaaagaa gctattgaga agtatgctag agctaacaaa ttatggcatg
     1201 ctcatttagg cgatccggtt tttaaagata cgtttcatgg gaaatacaag gtttctgact
     1261 gggttattca tttccagcgg ctgacaccga accatataaa gcttttagag cttggatacc
     1321 atgacccaat gaagctacct caaaactttg aatgatcgag cggcaggcta aatgaaatct
     1381 tgagattcat tcagtctcgt cgtaatctca ctattgtaaa aacgaaaaaa ccaccctggc
     1441 aggtggtttt tcgaaggtta gttaatcctg gcagattctc taaccgtggt aacagtcttg
     1501 tgcgagacat gtcaccaaat actgtccttt cagtgtagcc tcagttaggc cgccacttca
     1561 agaactctcg ttacatctct cgcacatcct gcttaccagt ggccgttgcc agtggcgtta
     1621 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1681 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaaccga
     1741 gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1801 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1861 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1921 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc ctcccgcgcg cccttcttct
     1981 gggtctttgt cttttgctca catgttcttt ccggttttat cccccgattc tgtggataac
     2041 cgtattaccg cctttgagtg agctgacacc gctcgccgca gtcgaacgac cgagcgtagc
     2101 gagtcagtga gcgaggaagc ggaagagcgc cttatgtgac attttctcct tacgctcctc
     2161 tatgccgttc tgcatcctgt ccggatgcgt tatatcccgg taagattttc cgcttcaaag
     2221 cgtgtctgta tgctgttctg gagttcttct gcgagttcgt gcagtttctc acacatggcg
     2281 gcctgttcgt cggcattgag tgcgtccagt ttttcgagca gcgtcaggct ctgacttttt
     2341 atgaatcccg ccatgttgag tacggcttgc tgctgcttat tcatcttttc gttttctccg
     2401 ttctgtctgt catctgcgtt gtgtgattat atcgcgtacc acttttcgac tgttttgctg
     2461 ccgctattct gccgcttggc tttttgacgg gcatttctgt cagacaacac tgtcactgcc
     2521 aaaaaactgc cgtgcctttg tcggtaattc gagcttgctg acaggacagg atgtgcaatt
     2581 gttataccgc gcatacatgc acgctattac aattgccctg gtcagggctt tgccccgaca
     2641 cccatgtcag atacggagcc atgttttatg acaaaacgaa gtggaagtaa tacgcgcagg
     2701 cgggctatca gtcgccctgt tcgtctgacg gcagaagaag accaggaaat cagaaaaagg
     2761 gctgctgaat gcggcaagac cgtttccggt tttttacggg cggcagctct cggtaagaaa
     2821 gttaactcac tgactgatga tcgggtactg aaagaagtta tgagactggg ggcgttacag
     2881 aaaaaactct ttatcgacgg caagcgtgtc ggggacaggg agtatgcgga ggtgctgatc
     2941 gctattacgg agtatcaccg tgccctgtta tccaggctta tggcagatta gcttcccgga
     3001 gagaaaactg tcgaaaactg acggtatgaa caccgtaagc tcccaaagtg atcaccattc
     3061 gctttcatgc atagctatgc agcgagctga aaacgatcct gacgcatcct tcctgttttt
     3121 ccggggtaaa acatctcttt ttgcggtgtc tgcgtcagaa tcgcgttcag cgc
//
