LOCUS       NZ_MW133282             2690 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Shigella flexneri 1b strain 422 plasmid pSF422-3, complete sequence.
ACCESSION   NZ_MW133282
VERSION     NZ_MW133282.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Nisa,I., Qasim,M. and Driessen,A.J.M.
  TITLE     Characterization of novel plasmid (pSF422-3) isolated from Shigella
            flexneri 1b 422 strain of Pakistan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Nisa,I., Qasim,M. and Driessen,A.J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2020) Microbiology, Kohat university of science
            and technology, Kohat, Kohat, KP 26000, Pakistan
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: TopHat2 v. 2.1.1
            Coverage                     :: 23516
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_023574255.1-RS_2024_06_19
            Annotation Date              :: 06/19/2024 11:05:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW133282.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /strain="422"
                     /serotype="1b"
                     /isolation_source="Pediatric diarrheal stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /plasmid="pSF422-3"
                     /geo_loc_name="Pakistan"
                     /collection_date="2016"
                     /collected_by="Department of Microbiology, Kohat University
                     of Science and Technology, Kohat, Pakistan"
     gene            498..659
                     /locus_tag="NBT71_RS00005"
     CDS             498..659
                     /locus_tag="NBT71_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007783.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     gene            1038..1661
                     /locus_tag="NBT71_RS00010"
     CDS             1038..1661
                     /locus_tag="NBT71_RS00010"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="WP_226342037.1"
                     /translation="MDVTSHRRLFQKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFT
                     RQKGDGWTVVPKNLRIDFYTKDNDFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKV
                     LANARNIPQSKLEEEMMSQAIYRKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKN
                     IDIAFITDKNHKKHLSDIFKVINESGVKYDVIHFGACRVNREEK"
                     /name="putative adhesin"
     gene            complement(1887..2210)
                     /gene="mobA"
                     /locus_tag="NBT71_RS00015"
     CDS             complement(1887..2210)
                     /gene="mobA"
                     /locus_tag="NBT71_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_004988908.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2496..2690
                     /locus_tag="NBT71_RS00020"
     CDS             2496..2690
                     /locus_tag="NBT71_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_039059175.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEIA
                     EELQNSIQTRFEAESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2220..2458)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.72e-122"
     Typing          44..570
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.085"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(1891..2210)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="7.599999999999999e-62"
ORIGIN
        1 cagtgtatcc tgctccagac aggatgcaga acggtatacc ggtgcgtaag gagaaaatgt
       61 cacataaggc gctcttccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
      121 cggcgagcgg tgtcagctca ctcaaaggcg gtaatgcggt tatccacaga atcaggggat
      181 aacgccggaa agaacatgtg agcaaaaagc cgaaggcagg ggcaaaggcc gcgccggtgg
      241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      361 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gtccgccttt ctcccttcgg
      421 gaagcgtggc gctttctcat agctcacgct gttggtatct cggcccggtg taggtcgttc
      481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      541 gtaactatcg tcttgagtcc aacccggtaa gacacgaatt atcgccactg gcagcagcca
      601 ctgataaccg aattagagga gtaagacttg aagtcatgcg cctgttaagg ctaaactgaa
      661 agaacaagtt ttggtgactg cgctcctcca agccagttac ctcggctcaa agagttgata
      721 gctctgagaa ccttcgaaaa accgccttgc agggcggttt tttcgtttac aggcagcaga
      781 ttacgcgcag aaaaaaagga tctcaagaag aacctttaat tttttttcac acttggtatc
      841 ctactttaac ccataaaata aacacttaca ccgacacacc cttaaacaaa agaaaaaaaa
      901 caataaaaaa caccgcaaaa atggattgtt aacaaatgaa aatactcaat tcgaatctca
      961 taacaaacat atcatcaaca ataagaaatc accttctaac atcatccaaa aatataaaaa
     1021 cacacccatc accaaacatg gacgtaacaa gtcatcgtcg tttattccag aaagacggaa
     1081 taggattgag ccaaacagga aaagcaacac aacttgtcat tatgtcgcat ggtggatgga
     1141 aagagatctc ccatccacaa acactattca cgcgtcaaaa aggcgatgga tggactgtag
     1201 tccccaaaaa cctaagaatt gatttttata caaaagacaa cgacttcaca aagggactaa
     1261 gcgttctttc agaggtaaac aaacgccata ctgaagctca aaaagggtta acaccatcac
     1321 taaacataag caaagatgac ctaaaagttc tagcgaacgc gcgcaatata cctcaatcaa
     1381 aattagaaga agaaatgatg tcacaagcca tatatagaaa agaatacgca acaagtaatg
     1441 agaagataaa aaactatgct ctatattacc atgaacaagc ccaaaatatt atacaaagac
     1501 atcaagatgg cttaggaaac aaaaatattg atatagcatt tataactgat aaaaatcata
     1561 agaagcacct ttctgacata tttaaagtaa taaatgaatc aggagtaaaa tatgatgtaa
     1621 ttcactttgg cgcttgccga gtaaatagag aggagaaata atcatcattt accataatcc
     1681 cttaattgta cgcaccactg aaacgcgctg aacgcgattc tgacgcagac accacaaaaa
     1741 tagatgtttt accccggaaa aacaggaagg atgcgtcagg atcgttttca gctcgctgca
     1801 tagctatgca tgaaagcgaa tggagatcac tttgggagct taccgtgttc ataccgtctg
     1861 ttttcaacag ttttctcccc gggaagctaa tctgccataa gcctggataa cagggcacgg
     1921 tgatactccg taatagcgat cagcacctcc gcatactccc tgtccccgat acgcttgccg
     1981 tcgataaaga gttttttctg taacgccccc agtctcataa cttctttcag cacccgatca
     2041 tcagtcagtg agttaacgtt cttaccgaga gctgccgccc gtaaaaaacc agaaacggtc
     2101 ttgccgcatt cagcagccct ttttctgatt tcctggtctt cttctgccgt cagacgaaca
     2161 gggcgactga tagcccgcct gcgcgtatta cttccacttc gttttgtcat aaaacatggc
     2221 tccgtatctg acacggggtg tcggggcgaa gccctgacca gggtaattgt aatagcgtgc
     2281 atgtatgcgc ggtataacaa ttacacatcc tgtcctgtca gcaagttcga attaccgaca
     2341 aaggcacggc agtttttttg gcagtgacag tattgtcagg cagaaatacc cgtcaatgcg
     2401 tcagacggca gaataccggc ggtaaaacag tcgaaaagtg gtacgcgata taatcacaca
     2461 acgcagatga cagacagaac ggagaaaaca gaaagatgaa caagcagcag caaaccgtac
     2521 tcaacatggc gggattcata aaaagccaga gcctgacgct gctcgaaaag ctggacgcac
     2581 tcgatgccga cgaacaggcc gccatgtgtg agaagctgca cgaaatcgcg gaagaactcc
     2641 agaacagcat acagacacgc tttgaagcgg aaagtggcac cggggtataa
//
