LOCUS       NZ_MW133279             3179 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Shigella flexneri 2b strain 203 plasmid pSF203-4, complete sequence.
ACCESSION   NZ_MW133279
VERSION     NZ_MW133279.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri 2b
  ORGANISM  Shigella flexneri 2b
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Nisa,I., Qasim,M. and Driessen,A.
  TITLE     Characterization of plasmid (pSF203-4) isolated from Shigella
            flexneri 2b 203 strain of Pakistan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Nisa,I., Qasim,M. and Driessen,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2020) Microbiology, Kohat university of science
            and technology, Kohat, Kohat, KP 26000, Pakistan
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: TopHat2 v. 2.1.1
            Coverage                     :: 24936
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_023574225.1-RS_2024_06_19
            Annotation Date              :: 06/19/2024 11:06:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MW133279.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri 2b"
                     /mol_type="genomic DNA"
                     /strain="203"
                     /serotype="2b"
                     /isolation_source="Pediatric diarrheal stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1288825"
                     /plasmid="pSF203-4"
                     /geo_loc_name="Pakistan"
                     /collection_date="2016"
                     /collected_by="Department of Microbiology, Kohat University
                     of Science and Technology, Kohat, Pakistan"
     gene            complement(169..333)
                     /locus_tag="NBT66_RS00005"
     CDS             complement(169..333)
                     /locus_tag="NBT66_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000090200.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSIQIR
                     FEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1214..2323
                     /gene="wzz(fepE)"
                     /locus_tag="NBT66_RS00010"
     CDS             1214..2323
                     /gene="wzz(fepE)"
                     /locus_tag="NBT66_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_005065596.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVIL
                     FFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFALF
                     IKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKNDKS
                     SLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEILALD
                     EIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATKLNI
                     KKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAIIVI
                     LSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            complement(2568..2702)
                     /locus_tag="NBT66_RS00015"
     CDS             complement(2568..2702)
                     /locus_tag="NBT66_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071606675.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY"
                     /name="replication initiation protein"
     Typing          3..89
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          403..501
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          626..883
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="89.0"
                     /evalue="3.4e-112"
     Typing          complement(2631..3157)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.956"
                     /coverage="98.0"
                     /evalue="2.2599999999999997e-176"
ORIGIN
        1 catctgctct gccgcctcat aattaagcca gtatacactc cgttatcgct acgtgactgg
       61 gtcatggctg cgccccgaca cccgctaaaa ccccctgacg cgccctgacg ggcttgtctg
      121 ctgacgaaaa cgcccacggg aaagccgtga ccgcctccgt cagcttcgtt atgttcctgt
      181 ttcactttcg gcctcaaagc gaatctggat gctgttctgg agttcttctg cgagttcgtg
      241 cagtcgtttg cacatggccg cctgttcgtc ggcatccaga gcatccagtt tttcaagcag
      301 tgtcaggctc tggcttttga tacatcgcgc catattcagc gcagtttgtt gttatttgtt
      361 caccttccgt tgttctccgt cagttccggg tgtcgggggg gggaccctga gcaggtggta
      421 attgtaagac gttgcgggcg acggtattac aattgcacat cctgtcctgt ttctttagga
      481 cgtatcatgg cacagcatat caactgataa ataatccctg tgcagtgcag ccgctgttgc
      541 ataaaatttc gctctgcgtc gagtgtttct taatcaataa ttccgctgat actccggaaa
      601 aacgcgagtc tgttctcatg cctgaaatgc ccacacccca cgcaaaaaca agtttttgct
      661 gatttttctt tataaataga gagttatgac aaattagttc ttcttgctct ctttgtgata
      721 tttaaaaaag cggtgtcggc gcggtgttgt agctgcgcca acaccgcttt ttaggggtgg
      781 tactgactat tttcataaaa aaaacattat cttatattag gggtgctgct agcggcgcgg
      841 tgtgtttttt tacaggacgc ccctgggggc gctgctaggg gtgtctgttc agatatgtag
      901 caatgagtca gggattcacg aactgaaaat gtccgaaaat gtgtctcatg ggcactgtat
      961 gcgcccaaac cgatcgtcat tcactttcat gcatagctat gcagtgagct gaaagcgatc
     1021 ctgacgcatt tttccggttt accccgggga aaacatctct ttttgcggtg tttgcggcag
     1081 aatcgcgttc agcgcgtttt agcggtgcgc gcaataaaat gttatgttaa atgtctgctg
     1141 aatggttttt aggttttttc ctaacatatg atagaataaa aaaaccatat gttaatatat
     1201 tgtgtttttt tcaatgaaaa tctcaaaggt ctctgacgaa catttttcta attacaacat
     1261 gttgccgaat ggtaatgaag aaattgacct ttttggcttg attagcgttt tatgggcagc
     1321 aaaaataaaa attgttgggg tgattctatt ttttgcttgt attggctttc ttgtttcttt
     1381 tgttttgcca caaaaatgga ctagttctgc tgtggttact cctgctgagt ctgttcaatg
     1441 gagtgaatta aataaggagc tttccaagct tcatgttctg ggagtggatt ttgatatcaa
     1501 tcgtgatagc gcatttgctc tgtttattaa gaaatttcag tccgttaatt cgttgaatga
     1561 gtatatgaga tcatctcctt atgttatgga gattattaaa aagaataaca ttagcgctct
     1621 cgaactgcat cgtgcaattg ttgagttgag taaaaatatg aagtcgattg atgataatgc
     1681 cagtaagaaa aacgacaagt catcattgta tgtatcatgg actctgagtt ttactgctcc
     1741 aacaagtaaa gaagctcacg atgtgttgtc tgggtatatt aattatgttt cttcccttgt
     1801 tgtaagggat ttgatggaag atataagaaa taaactagaa gttaaaacta atgttgaaaa
     1861 agaaattctt gcactggatg agataaaaat tagaaaccag ctgaatgcag atattcgacg
     1921 cctcaattat tcactggagg ttgctaatgc ggctggaata aaaaaacctg tatacagcaa
     1981 tggtcagatt atgaaggatg acccagattt tcctgtggct ctcggttctg atggtatagc
     2041 aactaaattg aacatcaaaa aatcaatcaa ggatgtttcg gaattgagtg gggagttgcg
     2101 aaatcgtcaa tatgttgtga atcaattggt tgtggcgaaa gtgggggatg ttgattttat
     2161 gccatttcag tatcaattga gcccaacgtt accggtcaga aaggatgggc caagtaatgc
     2221 cataattgta attctttctt ctgtcattgg agggatgctt gcatgtggct atatactttt
     2281 acgtcatgcc atggaggcca gaaagatgag tgtgattgag taaaagttgt gctgatgcat
     2341 cattttataa gcaactttta taaaaagttc aaaggatctt cttgagatca ttcccttaca
     2401 gccgtagtct tctactctag aaaacgaaaa aaccacctgg ggaggtggtt tttcgaaggt
     2461 tcagtaagtt ggggaacttc tgaaccgtgg taacagtctt gtgcgagaca tgtcaccaaa
     2521 tctgtccttc tagtgtagcc tcagtaaggc tgccacttca agaactctca atacatctct
     2581 cgcacatcct gtttaccagt ggccgctgcc aatggcgtta agtcgtgtct taccgggttg
     2641 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2701 acacagccca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     2761 cgaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     2821 cacgagggag ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac
     2881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2941 gccaccggcg cggccttttt tcggggcctc ttttttgctc acatgttttt tctggtgtta
     3001 tcccctgatt ctgtggataa ccgtatatcc gtctttgcgt gagcagacac cgctcgccgc
     3061 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg ccggaagctg
     3121 cattttctcc ttacgtatct gtgcagcatt tcacacctca tatgtcgctg ttctctcac
//
