LOCUS       NZ_MT560077            13636 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RIVM_C015274 plasmid pRIVM_C015274_3,
            complete sequence.
ACCESSION   NZ_MT560077
VERSION     NZ_MT560077.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Hendrickx,A.P.A., Landman,F., de Haan,A., Borst,D., Witteveen,S.,
            van Santen-Verheuvel,M.G., van der Heide,H.G.J. and Schouls,L.M.
  CONSRTM   Dutch CPE surveillance Study Group
  TITLE     Plasmid diversity among genetically related Klebsiella pneumoniae
            blaKPC-2 and blaKPC-3 isolates collected in the Dutch national
            surveillance
  JOURNAL   Sci Rep 10 (1), 16778 (2020)
   PUBMED   33033293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   Hendrickx,A.P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2020) Bacterial Surveillance and Response, RIVM,
            Antonie van Leeuwenhoeklaan 9, Bilthoven 3721 MA, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015511375.1-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 02:00:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 17
            CDSs (total)                      :: 17
            Genes (coding)                    :: 13
            CDSs (with protein)               :: 13
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT560077.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RIVM_C015274"
                     /db_xref="taxon:573"
                     /plasmid="pRIVM_C015274_3"
     gene            join(10680..13636,1..49)
                     /locus_tag="I0P83_RS00005"
     CDS             join(10680..13636,1..49)
                     /locus_tag="I0P83_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143775.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            <84..374
                     /locus_tag="I0P83_RS00010"
                     /pseudo=""
     CDS             <84..374
                     /locus_tag="I0P83_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            680..943
                     /locus_tag="I0P83_RS00015"
                     /old_locus_tag="KFDJIIFH_05340"
     CDS             680..943
                     /locus_tag="I0P83_RS00015"
                     /old_locus_tag="KFDJIIFH_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            933..1232
                     /locus_tag="I0P83_RS00020"
                     /old_locus_tag="KFDJIIFH_05341"
     CDS             933..1232
                     /locus_tag="I0P83_RS00020"
                     /old_locus_tag="KFDJIIFH_05341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1294..1671)
                     /locus_tag="I0P83_RS00025"
                     /old_locus_tag="KFDJIIFH_05342"
     CDS             complement(1294..1671)
                     /locus_tag="I0P83_RS00025"
                     /old_locus_tag="KFDJIIFH_05342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            1740..1919
                     /locus_tag="I0P83_RS00030"
                     /old_locus_tag="KFDJIIFH_05343"
     CDS             1740..1919
                     /locus_tag="I0P83_RS00030"
                     /old_locus_tag="KFDJIIFH_05343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1947..2477)
                     /locus_tag="I0P83_RS00035"
                     /old_locus_tag="KFDJIIFH_05344"
     CDS             complement(1947..2477)
                     /locus_tag="I0P83_RS00035"
                     /old_locus_tag="KFDJIIFH_05344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2484..3215)
                     /locus_tag="I0P83_RS00040"
                     /old_locus_tag="KFDJIIFH_05345"
     CDS             complement(2484..3215)
                     /locus_tag="I0P83_RS00040"
                     /old_locus_tag="KFDJIIFH_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(3215..5179)
                     /locus_tag="I0P83_RS00045"
                     /old_locus_tag="KFDJIIFH_05346"
     CDS             complement(3215..5179)
                     /locus_tag="I0P83_RS00045"
                     /old_locus_tag="KFDJIIFH_05346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6660..6809)
                     /locus_tag="I0P83_RS00050"
     CDS             complement(6660..6809)
                     /locus_tag="I0P83_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6894..7151)
                     /locus_tag="I0P83_RS00055"
                     /old_locus_tag="KFDJIIFH_05348"
     CDS             complement(6894..7151)
                     /locus_tag="I0P83_RS00055"
                     /old_locus_tag="KFDJIIFH_05348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(7161..>7745)
                     /locus_tag="I0P83_RS00060"
                     /old_locus_tag="KFDJIIFH_05349"
                     /pseudo=""
     CDS             complement(7161..>7745)
                     /locus_tag="I0P83_RS00060"
                     /old_locus_tag="KFDJIIFH_05349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            complement(7893..>8063)
                     /locus_tag="I0P83_RS00065"
                     /pseudo=""
     CDS             complement(7893..>8063)
                     /locus_tag="I0P83_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            complement(8118..8822)
                     /locus_tag="I0P83_RS00070"
                     /old_locus_tag="KFDJIIFH_05350"
     CDS             complement(8118..8822)
                     /locus_tag="I0P83_RS00070"
                     /old_locus_tag="KFDJIIFH_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_004217321.1"
                     /translation="MNPFKGRHFQRDIIRWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<8888..9103)
                     /locus_tag="I0P83_RS00075"
                     /old_locus_tag="KFDJIIFH_05351"
                     /pseudo=""
     CDS             complement(<8888..9103)
                     /locus_tag="I0P83_RS00075"
                     /old_locus_tag="KFDJIIFH_05351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            complement(9173..9727)
                     /gene="aac(6')-Ib"
                     /locus_tag="I0P83_RS00080"
                     /old_locus_tag="KFDJIIFH_05352"
     ARG             complement(9177..9727)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9961..10518)
                     /locus_tag="I0P83_RS00085"
                     /old_locus_tag="KFDJIIFH_05353"
     CDS             complement(9961..10518)
                     /locus_tag="I0P83_RS00085"
                     /old_locus_tag="KFDJIIFH_05353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     ARG             complement(8835..9091)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             complement(9174..9778)
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          5979..6557
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2488..3215)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 tcatctgaga ccattaaaag aggcgtcaga ggcagaaaac gttgcttaac gtgagttttc
       61 gttccactga gcgtcagacc ccttttgatc ccaatttttc cacccagaaa gctgacacaa
      121 cacgaatgat ggtgcctttt ttccggcagt tctgggatat gggcgcaaag gataaagcga
      181 ccggaaaatc acggagtgat gtgcagcaac gcattcagca gtttcacagt caggaatttt
      241 taaactcact ccaaggcaca acacaatttg cgggtaccga ttaccgcagc aaagacctca
      301 caccgaaaaa atcaaggcta ctggctgaca ccatttcagc tgtttatctc gatggctatg
      361 aaggcagaca gtaagtggat ttaccataac gcgtcattac gcgcaccgct cagacgggct
      421 cagggcgttt ccggcggcta cgggataaaa caggcaacaa cccccccgga aaactgccgg
      481 aaacgcgccc gataaatttt atcctaatgc atgcctatgc acgcaactgc atggcatatg
      541 ttttgacgta aaatggatgc cgtaagccct tttttaccga ctttctgctt catcagcctg
      601 actgacacga ccatattctt tcagaccgcc ccctgctaaa cttccttcat attgccgcat
      661 taacccggga ggctccgcaa tgagcaacac tacacactac gaaaacgcca actttcttcg
      721 ggaactggct gaaagcctgc cccgcatact gccggaaggt ggcccggaca aagctgcgct
      781 gttgcagcga ctggctaacg aagaactggc ccaggcagag tatgaagatc aggttcgcgc
      841 caaagtgacc gccgcccgtg ctgacacgcg tccgggtatg accacggaac aactccgcca
      901 gcgcctgcat ggccgttatc aggaactgcg cgatgccgta tgacgtggaa tggaagcagg
      961 gagccatcga ggatgtaacg acgctgtttg actatatcgc ggaaaattca tccctgtggg
     1021 atgcgcagaa cgttaccgac cgggttctcg ctgcggcgga caaactggcc gacttcccgc
     1081 gactctatga agtggactca cgctatggcg aggacgttcg ccggatcagt ctgatgggac
     1141 agcatgtcct ctatgatgtt gatgaccaga ccagaaaagt ccgggttctg gccgtggtcg
     1201 gccagcgcca gaatccccat attgttctct aataaccggt gacggggacg caattcattg
     1261 cgtggccgtc gccggttgga caagcacagc gcgtcagcgt tttccccacg gccaccaccg
     1321 gcgcggtgtt ccggccgggg tctctgctgt ctccggtttg tgttccagca gtctcatgga
     1381 ctgcatttcc tgccgtagcc cggccacctc ctccctgagt gcttttatct ccgcgatcaa
     1441 cgcgtcggag gccatttttt cagtctcacc cgacagtctc aactcgtccc gctcgtgagt
     1501 ctcatcctgt cttaactcac cgtacacccg gataagctcg ctggtatcca ccacccggcc
     1561 gccatcagct tccgtacggt atgatacgcg gcctttcgcc atatcccgat acagggaact
     1621 tcgggcttta cccgtcagtt ccgtcgcctg tctcaccgtc acccacgcca ttgtctcacc
     1681 ccgctatttt tgagacagta tacctgacag gcgcacacca tgcccggaag gaaagaatca
     1741 tgacaaaaca ggaaaaaaca gccctcaata tggctaaatt tattaaggag cagtcgctgc
     1801 tattgctcga taaactgaat gagctggata tggatacccc ggccgacatg tgcgagcggc
     1861 tacacgagga tgcggaaaca ctctggcagg tgatgcggga taagctcggg gaggaataac
     1921 agcaccggca tggccggtgc tggcatttat tttttgccga acggccacca ccggcgggca
     1981 ggtttgtcct ccggcagttc aggtaccggc acgttaaaat agcggctgtt atattcccgc
     2041 atcagctccg ctttcagctt ctcgttcacc gcgctttccc cgtgccgctc actcaccacg
     2101 tcaatcatcg cgttaatctg ctccggtgag cacttcccgg catcaaccac ctgcacaccg
     2161 ttctcgatgg cgaaccgctc caccttgcga tagaggtaat cacacagctt cattcctgtg
     2221 ggtttgttcc gttcccattg cggattttta ccagtgaaaa agtacgtggc tttttcgtcg
     2281 ctgttgagcc gctcgtcata catcccgatg gatttcatca tcgccagatg ctgggcccgg
     2341 tcgacaaaac agaaaacata cgccccggtc tgccaggcga gcaccaccat atcttccaga
     2401 tgctcgctgg tcggcatgat gcagcgctcg ccgttccggt cctccgcgtc ttcaataaat
     2461 accatgtaca gcgccataat ccctcactcc gttgtcataa atttaaagca cgcaaacaac
     2521 cgttcacgat ccagctctgc cgttttgcgg gcctcgccgg tctgtgggtt cacgatttca
     2581 tccaccgaca ggattgcccg ctgcaccctg acctgcgtcg cccggtccgg gcagatgtac
     2641 cagatttcat cgatgccgtt agctttgcgg ttgaacagat acccggacac cacttcctga
     2701 taccggcgac ggctcttgac ggtgcgctcc acctcaaacg ccaccgtttt accgtccagc
     2761 tccgccaggg cgtccggcac tttcatgcct ttaagactca gacggtgcag aatgcgccag
     2821 ccggtggccc ctctggcttc catatcaagg cgcagggatt gcacggccag ccggtgcggc
     2881 agatgtgctg ccgacaccct tgacggctga aacggaataa catccgccag cggttcgtcc
     2941 tcgtcgaacg agaacgccac gccatgcggc gtcagccccc acacgctgac ctgacggccg
     3001 tccaccggat atttggtccg gaccagcagc cctttcgcca ccaggctgtc gaggaagcgg
     3061 tagaccggct gcaccgccgc aatccccagc cgccgcgcaa ccagctccgc cgtggaacac
     3121 agctcttccc gcaaccagcg cagggtcagc cgggttttct ccgcgttccg cgcctgtcgt
     3181 ttcgcgtggc tgacgttgta cagctccagc gccatcagta aatccccgca aaatcatcat
     3241 ccgctttcgg cccgccgccg tcggccgggg gccggaactc cgccagggga tcggttttcg
     3301 ccagcaccgg gaaacgctgc aacgggattt cccggcgctc aacccgcagc ggactgctga
     3361 acgccatccg ggccacaccc agcccggtca tgaccgcgaa cccgtccggc aggtgttgca
     3421 gggtgttcac gtcaaacagc ggccgcgcct ccttctggat gaagctgtcc ccgctgatat
     3481 gctccacgtt ccccgcctcc cggctggcac ggcggcgctc cacgtccacc ctgatttccc
     3541 cggtctgccc cgccgcccac tgcgcggact ccaggctggc cgcccggtaa aaccagcgga
     3601 tcggcgtgtt atccagtacc gtggttttca caaattcagc gggcagatcc tgcccgcact
     3661 gcccgaaatc gcccagcgac tggtgcgcga gcaacagatt gcagtcccgg ctgcgcagcg
     3721 tccccagtgc gttcagtaca tacttcgaca gcagatattt aatttcatcg agcatcacgc
     3781 tgacgtgtgg ccactcccgg aactcgtccc gcgcaatgat gatttgcgcg cagcgggcaa
     3841 acagcatttt ttgcacacgg atcaccgcct cgtcgtccat cgagccgata acgtacaggc
     3901 agccgccgcc gttgataatc cccgccacat cgatgccact gtccgtctgc aggacgctca
     3961 ggtcagcgac gttttccagc ctagtgatca accccttcac gtcctttttc agggcctccg
     4021 gcagcgcgta cgccgccgcc agcacctgct gaatgtttgc cccctgcgga aactgctccg
     4081 caatcagctt cgccgctttc tgctcgctga tgcggtagtg gtccgccacg tcgccggtct
     4141 cgcccaggtt aagcccggca atcagcaggt ttttcagcgc gtactggtcg agatcgcgga
     4201 acagattcag ctgcgccggt ttccccggcc gcagatccag cagggtgaag tccggacagt
     4261 gcgcccggaa gacgtgcggc gcccatgcgt cccctttggg gtcgaagtac accaccgcgt
     4321 ccccgaaatc gcgtacgcag cgaatgagcg cgttggtggc catcacgctc ttgccgctcc
     4381 cgggcaggcc gaggatctga atgttggtct tgcgccaggt atcacgcgga accagcaccg
     4441 gcttgccctg ctcatcctta cccagaaaca ccgcctgctc ctgccgggcc tgctccgagg
     4501 aaaaatcggc cagcgcatta accggtaccg gctccccgaa atgcaggtgg cgcacgtcgg
     4561 tcatctctcc tgtccgctgg ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg
     4621 cctcgtcagc agccgcaaaa tgaccgtcaa gcagcaggaa cgccagcagc cgcgccaaca
     4681 caatcccgac agccagcccg cccgcgcacc aggcaatccc gaacaggtta gccggctgca
     4741 gcaggtaata atctgcgttt gcgtagaggt ccggacgcag gacggtttct ccccagctct
     4801 cgataaacac ggcgacaacc agccccccca gaagggccag accggccgcg ccggtcagcc
     4861 agtacgcgta gttccagaca tgtcgacgtg ccctgaccat ccgcccgggc gaatccagcg
     4921 aggccagcac cgaaaatggc gctggccgtt tattatcgcg cttatagcga tcggcatact
     4981 cccgtcgctc cttatgactc agccccacaa aacgcatccg tccggcccaa agttcagcca
     5041 gaccgaaaaa caggccgata actaggaaag ccccggccag accgtaatac tcctggtaca
     5101 gccagctgac ccacggataa atctgccggg ccagctccgg aatgtgccac ttccaggcaa
     5161 gcgaatccgt attaaacatg ttctctctcc ttcagacgat ggtttgccgg cgtgatgtgc
     5221 agcccccgct gcacaagccg cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt
     5281 ggtgattgcg ttcagctttc accacggagg ccagcctctt tccgccccat gcggaaaagg
     5341 cgcagccccg gaggaacaac gagcacgccc cagcgtgtga gaggaaaagg cggcacccca
     5401 gtgcggtctt ttccggtgtt ccggcccccg tcagagttcg ccatagcgag cggctgacca
     5461 cccgcaaaat caggagcagc tttgctgctt cgccaggtgc gtccagcacc gtggattttt
     5521 gtgggtggtc aggcgtgagc ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc
     5581 agccggacgg agccagaaag agggggagag accgtgcgtc ggtggcgtcc agccagcgta
     5641 agcaggggat atccccctga ggtcagccac gccctcgcgg ggatgacctg tatttctccg
     5701 taaggagaaa aaagcaacag agataggtta acgtaaaatg ttcagcgaaa aaccgatatg
     5761 attcacctat cctggtaatt tattatcagg gaatataaag acttaatcag gggacaatat
     5821 gccacatcag cgcgtccggt gtccgtcttg taggtgccgg ggaaattgcg cctcctcgct
     5881 cactcggtcg ctacgctccg gccgtgagac tgcggcgggc gctgcggaca catgcaaagt
     5941 tacccacaga ttccgtgaat aagcagggga ctaacatgtg aggcaaaacg gcaaggccac
     6001 gcaggtggcg tttttccata ggctccgccc tcctgactga gttcacataa acagacgctt
     6061 tttcggtgca tctgtgggag ccggtggctc aaccatgaat cagtcaggag tggcgaaacc
     6121 cgacaggact taaagatcat caccgtttcc gatgaatcgc tccctcttgc gctctcctgt
     6181 tccaaccctg ccgtttcccg gatacctgtt ccgcctttct cccttgcggg aagtgtggcg
     6241 ctttctcata gctcacactc tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg
     6301 gctgtaagca agaacccccc gttcagcccg actgctgcgc cttatccggt aactgttcac
     6361 ttgagtccaa cccggaaaag cacgcaaaac gccactggca gcagccattg gtaactggga
     6421 gttcgcagag gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc
     6481 ggctacactg gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta
     6541 agcagttccc caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt
     6601 tacagggcaa aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6661 tactgaaccg cgatccccgt cagttcagag gaggacgatg gtgccaccgt tccaccctga
     6721 acatcccgga tatagtttgc ctgacatgcg accagaagaa agcctgaaaa aatcaatata
     6781 aaaagaaaaa caacctttac ctttttcata taccttccct gtatttaaaa accccggtat
     6841 tcatccctga acaccggaag tctgtatttc aaatcatcct tgataacttt tgcttaccag
     6901 ttaccatccc ggtaaacaaa ggatacaaag taatcaaatt tactaatatc gattacattt
     6961 ttaaagtgtg gctgtaatat cgaaacccat gatttttcaa catcaaacca accattatta
     7021 atattatcct ttaaaggcat ccccagactt tcaatgacag aaccatcatc accgaagtct
     7081 tttgagtatt caccaccttt aaactcttcg gttttcttat caaaccaatg aatatgtaat
     7141 ttaagcccca tatttacctc ttaaagatat tttttaatgt ttcgttttgg atccggccct
     7201 ttaacctgct tacccgtttt tggatcgaat gccccgaggt gttcgccatc actggcgcgg
     7261 tacccttcaa gctcaccgtg ctgggaatcc cattcataaa ttttacgctt tttatcacca
     7321 taccatcggg cccgcttacc accaccacct tgtttagggg ttttaggttt accctctttc
     7381 aactctccca accccttaat atcttcagtc ttgggatcag gaaaataatc atggccgtaa
     7441 tctttagttc ccttacgagg ttttttcttt tcctcattta atttattttc tgcatccttc
     7501 gcttttttct ccttttgttt ccggctctcc atcgccccgg aaagcgcagc cttatccctg
     7561 gataaggatt cattcatttc gtttaaagat gtctgggcag cttttaattt ctcattctga
     7621 ttctgaatat ccttatcaag atagcgaacc tgatcctgcc acccggagcc atattcatga
     7681 ggcgggttat aagtcacaaa atcatcttta tttgcctctg cctcttttac ctttttatcg
     7741 gcttcggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8041 cgctcaccgg ctccagattt atcagggcac tgttgcaaag ttagcgatga ggcagccttt
     8101 tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat ttcgcccagg
     8161 ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat cacctcaata
     8221 cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc gccgattatc
     8281 cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat ctgtcggtgt
     8341 tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg accataggcg
     8401 ggcgctttat ccgtgttgat gaatcgcggg atctgccact tcttcacgtt gttgaggatt
     8461 ttacccagaa accggtatgc agctttgctg ttacgacggg aggagagata aaaatcgaca
     8521 gtgcggcccc ggctgtcgac ggcccggtac agatacgccc agcggccatt gaccttcacg
     8581 taggtttcat ccatgtgcca cgggcaaaga tcggaagggt tacgccagta ccagcgcagc
     8641 cgtttttcca tttcaggcgc ataacgctga acccagcggt aaatcgtgga gtgatcgaca
     8701 ttcactccgc gttcagccag catctcctgc agctcacggt aactgatgcc gtatttgcag
     8761 taccagcgta cggcccaccg aatgatgtca cgctgaaaat gccggccttt gaatgggttc
     8821 atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact atttgcaaca
     8881 gtgccgtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     8941 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     9001 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     9061 ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt aacgtttgac
     9121 atgaggggcg gccaagggcg ccagcccttg gacgtccccc tcgatggaag ggttaggcaa
     9181 cactgcgtgt tcgctcgaat gcctggcgtg tttgaaccat gtacacggct ggaccatctg
     9241 gggtggttac ggtaccttgc ctctcaaacc ccgctttctc gtagcatcgg atcgctcgca
     9301 agttgctcgg cgacgggtcc gtttggatct tggtgacctc gggatcattg aacagcaact
     9361 caaccagagc tcgaaccagc ttggttccca agcctttgcc cagttgtgat gcattcgcca
     9421 gtaactggtc tattccgcgt actcctggat cggtttcttc ttcccaccat ccgtccccgc
     9481 ttccaagagc aacgtacgac tgggcatacc caatcggctc tccattcagc attgcaatgt
     9541 atggagtgac ggactcttgc gctaaaacgc ttggcaagta ctgttcctgt acgtcagcaa
     9601 gtgtcgggcg tgcttcttct ccgccccacc actcgacgat atgagatcga tttagccact
     9661 catagagcat cgcaaggtca tgctcagtca tgaggcgcag tgtgacggaa tcgttgctgt
     9721 tggtcacgat gctgtacttt gtgatgccta actttgtttg gaaatgttga atactcatac
     9781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcactttt ccccgaaaag
     9901 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     9961 tcacgaggcc ctttcgtctt caagaatttt ataaaccgtg gagcgggcaa tactgagctg
    10021 atgagcaatt tccgttgcac cagtgccctt ctgatgaagc gtcagcacga cgttcctgtc
    10081 cacggtacgc ctgcggccaa atttgattcc tttcagcttt gcttcctgtc ggccctcatt
    10141 cgtgcgctct aggatcctcc ggcgttcagc ctgtgccaca gccgacagga tggtgaccac
    10201 catttgcccc atatcaccgt cggtactgat cccgtcgtca ataaaccgaa ccgctacacc
    10261 ctgagcatca aactctttta tcagttggat catgtcggcg gtgtcgcggc caagacggtc
    10321 gagcttcttc accagaatga catcaccttc ctccaccttc atcctcagca aatccagccc
    10381 ttcccgatct gttgaactgc cggatgcctt gtcggtaaag atgcggttag cttttacccc
    10441 tgcatctttg agcgctctga tctgaatatc gagggactgc tggctggttg agacccgcgc
    10501 ataaccaaaa attcgcataa aaatgtacct taaatcgaat atcagacacg atgtgtctat
    10561 tatgccaaaa tgacgattta atggacactc aaacgaagcc gttttactat gtctgataat
    10621 ttataatatt tcgaacggtt gcagttgtgt taaaaaagcc gtcaggcagg gaggccgata
    10681 tgcccgttga ttttttgacc actgagcagg ttgagagtta tggcaggttc actggcgaac
    10741 ccgatgaact tcagctggcg cgttattttc atcttgatga agcggataaa gaatttatcg
    10801 ggaaaagccg gggtgatcac aatcgacttg gtattgccct gcaaatcggg tgtgtgcgtt
    10861 ttctgggcac ttttcttact gacatgaatc atattccttc cggcgtccgg cattttaccg
    10921 ccagacagct cgggattcgt gatatcaccg ttcttgcaga atacggtcag agggaaaata
    10981 cccgccgtga gcatgcagcg ctgatacgtc agcactatca gtatcgtgaa tttgcctggc
    11041 cctggacatt tcgccttacc cgtcttttat atacccggag ctggataagc aacgaacgtc
    11101 ctggcctgct tttcgacctg gcgacagggt ggcttatgca acatcgtatt attctccccg
    11161 gagccaccac gctgacccgg ttgatttcag aggtaaggga aaaggcgacg ttgcgcctgt
    11221 ggaacaaact ggcactgata ccgtcagccg aacagcgttc acagctggag atgctgctgg
    11281 ggccaactga ttgcagccgc ctgtctttac tggaatcact gaaaaaaggc cctgtgacca
    11341 tcagtggtcc ggcgtttaat gaagcaattg aacgctggaa aactctgaac gattttggcc
    11401 tgcatgctga aaacctgagt acactcccgg ctgtgcgcct gaaaaatctc gcacgttatg
    11461 ctggtatgac ttcggtgttc aatattgcca ggatgtcacc gcagaaaagg atggcggttc
    11521 tggttgcctt tgtccttgca tgggaaacgc tggcgctgga tgatgcactg gacgttctgg
    11581 acgccatgct ggccgttatc atccgtgacg ccagaaagat tgggcagaaa aaacggctcc
    11641 gctcgctgaa ggatctggat aaatctgcat tggcgctcgc cagcgcatgt tcgtacttgc
    11701 tgaaagaaga aacaccggac gaatcgattc gtgctgaggt gttcagctac atccctaggc
    11761 aaaagctggc tgaaatcatc acgcttgtcc gtgaaattgc ccggccctca gacgataatt
    11821 ttcatgacga aatggtggag cagtacgggc gcgttcgtcg tttcctgccc catctgctga
    11881 ataccgttaa attttcatcc gcacctgccg gggttaccac tctgaatgcc tgtgactacc
    11941 tcagccggga gttcagctca cggcggcagt tttttgacga cgcaccaacg gaaatcatca
    12001 gtcagtcatg gaaacggctg gtgattaaca aggaaaaaca tatcacccga aggggataca
    12061 cgctctgctt tctcagtaaa ctgcaggata gtctgagacg gagggatgtc tacgttaccg
    12121 gcagtaaccg gtggggagat cctcgtgcaa gattactaca gggtgctgac tggcaggcaa
    12181 atcggattaa ggtttatcgt tctttggggc acccgacaga cccgcaggaa gcaataaaat
    12241 ctctgggcca tcagcttgat agtcgttaca gacaggttgc tgcacgtctt ggcgaaaatg
    12301 aggctgtcga actcgatgtt tctggcccga agccccggtt gacaatttct cccctcgcca
    12361 gtcttgatga gccggacagt ctgaaacgac tgagcaaaat gatcagtgat ctgctccctc
    12421 cggtggattt aacggagttg ctgctcgaaa ttaacgccca taccggattt gctgatgagt
    12481 ttttccatgc cagtgaagcc agtgccagag ttgatgatct gcccgtcagc atcagcgccg
    12541 tgctgatggc tgaagcctgc aatatcggtc tggaaccact gatcagatca aatgttcctg
    12601 cactgacccg acaccggctg aactggacaa aagcgaacta tctgcgggct gaaactatca
    12661 ccagcgctaa tgccagactg gttgattttc aggcaacgct gccactggca cagatatggg
    12721 gtggaggaga agtggcatct gcagatggaa tgcgctttgt tacgccagtc agaacaatca
    12781 atgccggacc gaaccgcaaa tactttggta ataacagagg gatcacctgg tacaactttg
    12841 tgtccgatca gtattccggc tttcatggca tcgttatacc ggggacgctg agggactcta
    12901 tctttgtgct ggaaggcctt ctggaacagg agaccgggct gaatccaacc gaaattatga
    12961 ccgatacggc aggtgccagc gatcttgtct ttggcctttt ctggctgctg ggataccagt
    13021 tttctccacg cctggctgat gccggtgctt cggttttctg gcgaatggac catgatgccg
    13081 actatggcgt gctgaatgat attgccagag ggcaatcaga tccccgaaaa atagtccttc
    13141 agtgggacga aatgatccgg accgcaggct ccctgaagct gggcaaagta caggcctcag
    13201 tgctggtccg ttcattgctg aaaagtgaac gtccctccgg actgactcag gcaatcattg
    13261 aagtggggcg catcaacaaa acgctgtatc tgcttaatta tattgatgat gaagattacc
    13321 gccggcgcat tctgacccag cttaatcggg gagaaagccg tcatgcagtt gccagagcca
    13381 tctgtcacgg tcaaaaaggt gagataagaa aacgatatac cgacggtcag gaagatcagt
    13441 tgggagctct ggggctggtc actaacgccg tcgtgttatg gaacactatt tatatgcagg
    13501 cagctctgga tcatctccgg gcgcagggtg aaacactgaa tgatgaagat atcgcacgcc
    13561 tctccccgct ttgccacgga catatcaata tgctcggcca ttattccttc acgctggcag
    13621 aactggtgac caaagg
//
