LOCUS       NZ_MT560068             4938 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RIVM_C014947 plasmid pRIVM_C014947_5,
            complete sequence.
ACCESSION   NZ_MT560068
VERSION     NZ_MT560068.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Hendrickx,A.P.A., Landman,F., de Haan,A., Borst,D., Witteveen,S.,
            van Santen-Verheuvel,M.G., van der Heide,H.G.J. and Schouls,L.M.
  CONSRTM   Dutch CPE surveillance Study Group
  TITLE     Plasmid diversity among genetically related Klebsiella pneumoniae
            blaKPC-2 and blaKPC-3 isolates collected in the Dutch national
            surveillance
  JOURNAL   Sci Rep 10 (1), 16778 (2020)
   PUBMED   33033293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Hendrickx,A.P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2020) Bacterial Surveillance and Response, RIVM,
            Antonie van Leeuwenhoeklaan 9, Bilthoven 3721 MA, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015511185.1-RS_2024_05_08
            Annotation Date                   :: 05/08/2024 04:51:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 5
            CDSs (with protein)               :: 5
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT560068.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RIVM_C014947"
                     /db_xref="taxon:573"
                     /plasmid="pRIVM_C014947_5"
     gene            complement(join(4861..4938,1..138))
                     /locus_tag="I0P72_RS00005"
     CDS             complement(join(4861..4938,1..138))
                     /locus_tag="I0P72_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(145..636)
                     /locus_tag="I0P72_RS00010"
                     /old_locus_tag="PMIDBGBA_05660"
     CDS             complement(145..636)
                     /locus_tag="I0P72_RS00010"
                     /old_locus_tag="PMIDBGBA_05660"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<671..1375)
                     /locus_tag="I0P72_RS00045"
                     /pseudo=""
     CDS             complement(<671..1375)
                     /locus_tag="I0P72_RS00045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1314..1637)
                     /locus_tag="I0P72_RS00020"
                     /old_locus_tag="PMIDBGBA_05661"
     CDS             complement(1314..1637)
                     /locus_tag="I0P72_RS00020"
                     /old_locus_tag="PMIDBGBA_05661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2646..3932)
                     /locus_tag="I0P72_RS00025"
                     /old_locus_tag="PMIDBGBA_05663"
     CDS             complement(2646..3932)
                     /locus_tag="I0P72_RS00025"
                     /old_locus_tag="PMIDBGBA_05663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_225986332.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDSLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(4004..4231)
                     /locus_tag="I0P72_RS00030"
     CDS             complement(4004..4231)
                     /locus_tag="I0P72_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039697198.1"
                     /translation="MPVNACGAALVLPRLIDASFSAGAGGFAVNMNIFLLFSPLKMAGF
                     LRVKSGAGHHAGMAEERAFSRPVAVIYNAT"
                     /name="hypothetical protein"
     gene            complement(4757..>4930)
                     /locus_tag="I0P72_RS00040"
                     /pseudo=""
     CDS             complement(4757..>4930)
                     /locus_tag="I0P72_RS00040"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     Typing          complement(1753..1925)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          1850..2396
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(3..1324)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.097"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1321..1637)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="2.38e-59"
ORIGIN
        1 caccgcgttc tcccgttgca taagtccaca catgctccgc cattccgcga aggcgctctc
       61 ccattcgtcc agcctttgcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg
      121 cttttccagc tctgtcattt tccgctactc ctgtttcagg cggatccagg tcgtcccgtc
      181 gtaggggatg gcttcggctt gcatccccgg cggcaggata aggaacctcc ggccgtcgtt
      241 gctctcctga tagcgtactc cccacgtccg ggcggtcatt ttcgccaggg tatcttcctg
      301 ctgacgaaga tgtgtgtaat tgtcggttat ctgccgtccc tgccaccaca gaatgctccc
      361 gctcgtggcg atcagcagcg ctgacaccat caggatggtg agccatgtcc tcccggccgt
      421 cagcaacacg ctccgccggt ttttctccat cgcctcgctc atactctgct cgtgggcgct
      481 gatggcgtcg ctgattctcc tcgcgcttgc gttcagttcc gctctgacag actgctcgtg
      541 ctcgctgaat gcggctttca gcatctcgcc ggtactcttc tgctgcgctt tcgattgctg
      601 ctctaagtcc ttcgccagcg ttaaaagact gctcatagat ggctcccttc agtcggatgt
      661 ttcgcccccc gtcagggtcg gcaatgctga tactgctttt cgttgtacgc accacctcaa
      721 aacctgcacc ttcaagagcc tcagtgacgt cctgacggct tttcagctcc ccggacgagg
      781 ctaagctcag cagtccacgg gtgatggctt ctgcggcttc ctgcttcgtt ttcggcagtc
      841 cggccggcgt gaccagcgcc cggcggtttt ccggcgcgtt cggatcgtgc agccccagtc
      901 tgccgttcac gatggtctgc caggcatcga tacgcggccg gtcggcgcgg tcgtagtacg
      961 gctggagccg cctgccggtc agcagctcgg tattggggat cagaaagttc agctccagac
     1021 gccccttgtc ctcgtgctcc acccacagaa tgctgtactg atctttatca agtccgggca
     1081 tcagaacccg ctcgaagctg gccatcagtt tttcccgctg gccgggaggc agatcggcct
     1141 ccgcaaacga cagtacgccg gaggtgtatt tcttcacgta aggcgacgcg tcgatcagct
     1201 cccggacttc ttccggcttc ccctgcagga cgctggcgcc ttcacgctgc cggtctttac
     1261 ccagcagata gtccaccggc ccggcgccac cgccgcgacc gcgtggatga aatttaacga
     1321 tcatcgtccg ttcctttctc aagaacggcg tgccgcagcc gctcaagccc ggcatcgatg
     1381 gccatcagcg ccgccacaat ctgcacccgg tcatgaccgg taccgacgcc ggcgttaaca
     1441 cggcgggcta tctggttcag gttgttgccc ataccggcaa gctggcgcag cagcgccggc
     1501 gagatcgaag gaagttttgc ggtacgagcc ggcttttcgt ccaggcaggt ctgccgcatc
     1561 cacgccgcca gctgcctgcc gtcgcagcgc tcaagtagcc gcctgtgctc atcctccgtc
     1621 acccacatgg tgagcatctt gttccgtttc tctgacaaaa cagtctcctg ataaccccgg
     1681 gacgggcggg aaaacctttc ccggtcggcg cggggttgga caagccgcag gcgcgtcagt
     1741 ggctgttagc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta gcggagtgta
     1801 tactggctta ctatgttggc acggatgcga atatcagtga agtgcttcat gtggcaggag
     1861 aaaaatgcag cagcggagcg tcagcagaat atgcgataca ggatatattc cgcttcctcg
     1921 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacaggcgg
     1981 ggcggaaatt tcctgggaga tgccagaaag aagcttaaca gggaagcgat aaggccgcgg
     2041 cgaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
     2101 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggtggct
     2161 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     2221 cgcgtttgtc tcattccacg cctgacactc agttccggga aggcagttcg ctccaagctg
     2281 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcaa
     2341 cttgagtcca atccggaaaa acacgacaaa gcgccactgg cagcagccac tggtaacagg
     2401 tttagaggag gagagtcttg aagtttcggg tgcgataagg ctacactgaa agaacagttt
     2461 tggtgactgt gctcctccaa gccagttacc gcggttcaaa agagcgtcaa ctcttgcgaa
     2521 ccttcgaaaa agccaccttg ccgggtggtt ttttcgtttt cagagcaaga gattacgacg
     2581 caacggaaag gatctcaaga agatcatctg tgatgttcga cagatatggg gatatgcacc
     2641 atggcctatc cggctttaac tgaaatggcg acatccttaa ccgcagtctc aacccctgca
     2701 gcattcctct gaatgctggt cataacttca cccgcttcgc gcatgagtgt aaccccttcc
     2761 cgagtgttca tcaggctgtt ttcaatgcca cagagaacat ccttggccag ttgactattg
     2821 ctggccacga cttgctcaat ttcttcagtc gcgctactga cgctcgccgc aagattacgc
     2881 acttctgcgg caacaaccgc aaaacttctt ccggatgcgc cagcccttgc tgcttcaatc
     2941 gcggcattca gtgcaataag tcttgtctgc atggcaaact tccggatggt ttccaccata
     3001 tcgtcaatac tttcagactg attgttgagc cgtgagatat cggtggagac agcccccatc
     3061 ccctgcgcaa ttctgtcaat cataaggatg ctattttcaa taacattggc accgttctgc
     3121 gcactttctc gggtctgcac ggccatatcc catgcatgca cagcagcctc ttgctctctc
     3181 tggtttcgca ggacatcagc cgtaacatct gtagcgaatt tcacaatttt ataaagctgc
     3241 ccatcactgt taaagacagg gttataagtt gcacggaacc acagagagtc tccctgacgg
     3301 ttaaggcgtg gaaacagacc agaaaaaaac tcaccttgat tcagactctc ccagaaatgt
     3361 cggtattcat cacttttgta aagcgtctcg gcacagaaca gacgatgatg cttacctatg
     3421 atttcatccc gcttatagcc agtggcattg acgaaatttt catttgcttc aagcacgatc
     3481 ccttcaggat tgaaggtgat gatggccata gaacggttta tggcatttac gactgcttcc
     3541 tgctcaagag cagaatgcac gcgctcagaa atatcggcag caattttaat gactctgata
     3601 acacggccgc gcctgtcaga aacggggata taactggctt caagccatac ggaacgattt
     3661 ccctttgcca gccgcaggta cttgccacta aagctttctc ccctggcaag gcgctgccag
     3721 aactggctgt attccggtga acttaccagc gagggcgggc agaatatacg atggtggtga
     3781 ccgattattt catcagccct gtaccccata gtggagagaa aaagcgggct ggctttattt
     3841 atcaccccct ccggactgaa ctcaatgaca ggcttattct gacaaattgc gtcatacgaa
     3901 atggctgatc gctggaggtt cagggagaac atatttttaa gtgaaaacat atgaatcaga
     3961 ttgcccggat aatgtaagta ctgccatggt tatcggctac tccttatgta gcgttatata
     4021 ttactgcgac agggcgggaa aaagcccgct cttcagccat gccggcatga tgtccggctc
     4081 cggatttcac gcgtaaaaaa cccgccatct ttagcgggct gaacaataag aaaatgttca
     4141 tatttacggc aaatccgccg gctccagcag agaagctggc gtctatcaat cttggaagaa
     4201 ccagcgcagc tccgcatgca ttaacaggca tcagaaggca aaaaaatggc tcacatccgg
     4261 gagccggaaa acactcaact gcctggatcg cagcacatcc tttgtgaagg ataatttaag
     4321 tatcggccgg attgccgcgg gcataagccc ggccggcata attttttccg tatccgccag
     4381 acctcggctt ttaacataac accccttgcc cgcaccgcag aaacggggtt ggctgcgttt
     4441 ccggtgccag acgcgagaaa gtggcgacag acatgacgaa aaaacaccaa aaacgcagcc
     4501 gctggatatt acactgatgc atagtcatgc agccagatgc atgcagcgta aagtcacact
     4561 aaaatcagcg cgaacgcctt aaaaaccggc aaatgcctct ccggcaactc acacagctcc
     4621 atcaccaggt aaaaacagtt cagcagggct tcaataacgt tgagaatttt catcacttcc
     4681 tccgggataa gtcactacca ttccggtagt cagcctattt agcaggaacg cgatgagccc
     4741 ttcttcgtgc gggctgtcac ctcatcttcg gaccatcgaa actcttctgc ttctgtcgga
     4801 gcgtcagctc cagctcgttc tgacgctgat actccagtgc agccttctcc tcgatggcat
     4861 tcagttcccg gacaagcggc gcagctgctc gcttaaactc agcacctgcg tgctcaagtc
     4921 cgacacgcgc tcgttcag
//
