LOCUS       NZ_MT560067             5596 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RIVM_C014947 plasmid pRIVM_C014947_4,
            complete sequence.
ACCESSION   NZ_MT560067
VERSION     NZ_MT560067.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hendrickx,A.P.A., Landman,F., de Haan,A., Borst,D., Witteveen,S.,
            van Santen-Verheuvel,M.G., van der Heide,H.G.J. and Schouls,L.M.
  CONSRTM   Dutch CPE surveillance Study Group
  TITLE     Plasmid diversity among genetically related Klebsiella pneumoniae
            blaKPC-2 and blaKPC-3 isolates collected in the Dutch national
            surveillance
  JOURNAL   Sci Rep 10 (1), 16778 (2020)
   PUBMED   33033293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Hendrickx,A.P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2020) Bacterial Surveillance and Response, RIVM,
            Antonie van Leeuwenhoeklaan 9, Bilthoven 3721 MA, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015511175.1-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 02:03:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT560067.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RIVM_C014947"
                     /db_xref="taxon:573"
                     /plasmid="pRIVM_C014947_4"
     gene            complement(274..432)
                     /locus_tag="I0P74_RS00065"
     CDS             complement(274..432)
                     /locus_tag="I0P74_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1072..1248
                     /locus_tag="I0P74_RS00005"
                     /old_locus_tag="PMIDBGBA_05651"
     CDS             1072..1248
                     /locus_tag="I0P74_RS00005"
                     /old_locus_tag="PMIDBGBA_05651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1238..1582
                     /locus_tag="I0P74_RS00010"
                     /old_locus_tag="PMIDBGBA_05652"
     CDS             1238..1582
                     /locus_tag="I0P74_RS00010"
                     /old_locus_tag="PMIDBGBA_05652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1791..>2225
                     /locus_tag="I0P74_RS00055"
                     /pseudo=""
     CDS             1791..>2225
                     /locus_tag="I0P74_RS00055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2260..2769
                     /locus_tag="I0P74_RS00020"
                     /old_locus_tag="PMIDBGBA_05653"
     CDS             2260..2769
                     /locus_tag="I0P74_RS00020"
                     /old_locus_tag="PMIDBGBA_05653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2776..2988
                     /locus_tag="I0P74_RS00025"
                     /old_locus_tag="PMIDBGBA_05654"
     CDS             2776..2988
                     /locus_tag="I0P74_RS00025"
                     /old_locus_tag="PMIDBGBA_05654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3364..3492)
                     /locus_tag="I0P74_RS00060"
     CDS             complement(3364..3492)
                     /locus_tag="I0P74_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            3655..3879
                     /locus_tag="I0P74_RS00030"
                     /old_locus_tag="PMIDBGBA_05655"
     CDS             3655..3879
                     /locus_tag="I0P74_RS00030"
                     /old_locus_tag="PMIDBGBA_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3863..4129
                     /locus_tag="I0P74_RS00035"
                     /old_locus_tag="PMIDBGBA_05656"
     CDS             3863..4129
                     /locus_tag="I0P74_RS00035"
                     /old_locus_tag="PMIDBGBA_05656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4199..4549)
                     /locus_tag="I0P74_RS00040"
     CDS             complement(4199..4549)
                     /locus_tag="I0P74_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4615..5160)
                     /locus_tag="I0P74_RS00045"
                     /old_locus_tag="PMIDBGBA_05658"
     CDS             complement(4615..5160)
                     /locus_tag="I0P74_RS00045"
                     /old_locus_tag="PMIDBGBA_05658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(5186..5512)
                     /locus_tag="I0P74_RS00050"
                     /old_locus_tag="PMIDBGBA_05659"
     CDS             complement(5186..5512)
                     /locus_tag="I0P74_RS00050"
                     /old_locus_tag="PMIDBGBA_05659"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          906..986
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.476"
                     /coverage="98.0"
                     /evalue="3.1e-22"
     Typing          complement(324..721)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1573..3104
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggtttgtac atgatgtgca gtgcgctgag ggcatcagtg gcgggtgttt ttccgtggga
       61 tggatggagc tccggggata acagaactga cgatcattta aaggatcttc ttgagatcct
      121 ttttttctgc gcgtaatctc ttgcactgta aacgaaaaaa ccgcctgggg aggcggtttg
      181 atcgaaggtt aagtcagttg gggaactgct taaccgggta actgggtata cgagaccgca
      241 gataccaaaa ctgttctttc agtgtagccg cagttaggca cccacttcaa gacctctcaa
      301 tatctctctc gtatatatcc agttaccaat ggctgctgcc agtggcgttt ttgcgtgtcc
      361 gtccgggttg gactcaagat gatagttacc ggaaagggcg cagcagtcgg gctgaacggg
      421 gggttcgtgc atacagccca gcttggagcg aacgacctaa accgagccga gataccagca
      481 gcgtgagcta tgagaaagcg ccacgcttcc cgaaagggaa aaaggcggaa caggtatccg
      541 gtaaacggca gggtcggaac aggagagcgc acgagggagc cgccggagag aaatcaccgg
      601 catctttaag tcctgtcggg tttcgtcctc ctctgacttg agcgtccatt tctgtgatgc
      661 tcgtcagggg aggcggagcc tatggaaaaa cggcaacgcc gcatcctttt ctccggccct
      721 ttctgatttg agcgcctttg ctttatcccg ttatccgtga gtcggtcacg cccgccgcag
      781 ccgaacggca ggagcgcagc gaccgagtga gcgaggaggc gtcatttcat ctgtccgtgc
      841 atggtattgc ggacgctcct gagccacata tcacacctgc cacatgaggc actttcatga
      901 atcgcgctct catgccaaca tagtaagcca gtatacacag cgtaaacgct gtgactggtt
      961 cagggctgcg ccccgaaacc cgctaacacc actgacgcgc tggcgcttgt ccaacgccgg
     1021 agcgacccgg aaaacttttc cgtgccgtcc ggcgtttgcg aaggagggat aatgcagaag
     1081 ccgaagaaga agccgctcgt cgtaaaaaaa agatcctggc atccggcagt gatggcaaat
     1141 ctgatgcgtc aggcacagga ggaagattac cgccgggagc agatggcaga gcttgagcgt
     1201 tcagcccggt cacattttgc ggatatcgga ggagaggatg gcggttaagc gtacccgttt
     1261 tttaggtatc cgggtgaccg atggggagta ccagcagctg ctggagcgct gcaatggaag
     1321 gcaactggcg gtgtggatgc gggaaacctg tctcgatacg cgcccggcgc ggtcgttgcg
     1381 gctgccgtcc atcgatccgg ttttgctgcg ccagcttgcc gggatgggga acaacctcaa
     1441 ccagatcgcc cggaaaatta acggcggcca gtggtcaggc gctgaccggg ttcaggtggt
     1501 ggccgcgctg atggccattg atgccgaact cgagcggctg cggcatgccg tgctggaaaa
     1561 gggggcagac gatgatcgtt aagtttcatc cgcgagggcg aggcggcggt gccgggccgg
     1621 tggattatct gctggggaaa gaccgccagc gcgacggggc catcgtcctg cagggaaagc
     1681 cggaggaggt ccgggagctt atcgacgcct cgccctacgc caaaaagtac acctccggcg
     1741 tcctgtcttt tgctgaacag gatttaccgc ccggccagcg tgaaaagctg atggcgagct
     1801 tcgagcgggt tctgatgccc ggactcgata aagaccagta cagcgtgctg tgggttgaac
     1861 accgggacaa ggggcggctg gaactgaatt ttctggtacc gaacacggaa ctgctgaccg
     1921 gcaggcgtct ccagccgtac tacgaccggg cagaccgtcc gcgcatcgat gcgtggcaga
     1981 ccgtggtgaa cgggcgactg gggctgcacg acccgaacgc accggagaac cggcgcgcgc
     2041 tggtgacgcc atcttcgtta cccaaaacga agcaggaagc cgcagaggct attacgcggg
     2101 gcttactggc cctcgcctcg tcaggggagc ttaaaacgcg tcaggacgtc actgaggcgc
     2161 tggaaagcgc aggttttgag gtcgtgcgca ccacaaagag cagcatcagc attgccgacc
     2221 cggacggagg gcgaaacatc cgacttaagg gagccatcta tgaacagtct tttaacgctg
     2281 gcgaaggact tagagcagaa atcgaaagcg cagcagcaga gtaccggcga aatgctgaaa
     2341 gccgcattca gcgagcacga gaagtctgtc agagcggaac tgagcgaaag cgggaagaga
     2401 atcagcgccg ccatccgcgc ccacgagcag ggtatgagcg cggccatgca gtcgaaccgc
     2461 ctgagcgtga tgcgtatggt cgggcggaca tggctgacca tcacgatggt gtcaggactg
     2521 ctgttcgcca gcctgagcgg ggtgttgtgg tatcagggga gcttgatagc gtcaaatctg
     2581 gccgaaatcg accggcagaa cgcagcgctg tcgaagctga acgcgaagac ctggggcgtg
     2641 acgtacctgg aagacagcaa cggacgtttt ctggtgctgc cgaaggggac ggccgtagac
     2701 aggacgcaga gctggacggt cgggaacggg cggtcgaagc agaacgcgct gagactggtg
     2761 aaggagtaag tgccgatgac cgagctggaa aaacagttgc tgagcgcatt agagcagcta
     2821 cagcaggact actcgaaaag gctggacgag tgggagagcg ccttcgggga atggcggaaa
     2881 atgtcagggc ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtgac gcgcttgagt
     2941 cagcaggtgg aacgcttgag cgggcaactg cagcgtttga gccggtagtg cagcgtcatg
     3001 agatggccgt ggccgcagaa cgggcgcacg agcaacggca gcacgaaaag gaactgacgg
     3061 aagcgcgttc ccggcagcga agctatgacg gtccgtcgct gggctgaaat gcaggaaaac
     3121 agggttctca tcgggcattt acgctgaaac cggatgtcat tcacctggct gcatggctat
     3181 gcagccaggt aaaatttatc tgtcgcgaac ggggcggttt atcgggtggt ttgttgccgg
     3241 ttttacctga ctgccacccc gttcgcggcg aacccgtccg gggcggtgcg ggcaacgggt
     3301 gttatgttta atcagcgtga acggcagata caaaaaagcc ccggagggct tttaactact
     3361 gacttatttt tttgaagatt tgctgggttt caatttgtca gtatttcccg gcgttttttt
     3421 attgtcacgc tggcgtttat caggttcccc tgttgaatcg gcaattcgtt tcggacccgg
     3481 cttaagaccc ataatcgtta tcctttcaat agttaagagg aaagccctca agctagatat
     3541 attgaccgcg ttacggatta tcaagagggg ctaacaagat tattttttgt tcttttaggt
     3601 tgattgtggt actgttgata tacatgtata gcaaatggtg ttcgggaggt ttatatgctt
     3661 gctatccggt tatctgatga gattgagtcc cgtctggact cgctggcgaa gcaaaccggc
     3721 agaacaaaga cgttttatgc gcgggaagca atactggcgc atctggaaga cctggaggat
     3781 tattatcttt cagcagaaac tgctgcacgc gttcgccgtg gtgatgaagc agtgcattcg
     3841 tctgaagacg tgaggaagtc acttggtctg gacgattaac tattccgatc gggcgctcaa
     3901 atcgttacgc aagatggaca aacagaacgc acgacggatt gtggatttta tgagtttacg
     3961 cattgcagtt gctgccgatc ctcgccagtc agggaagccg ctcaaaggtg agctgggcga
     4021 gttctggcgc tatcgggtgg gagattatcg cgttctgtgt gagatccgag atgacgagct
     4081 tgttatcctt gccgccacga ttggacatcg ccgcgaagtt tacgactgaa gcccgctatg
     4141 cgggcttttt tgatgccgac aacaggtatt atggtaaata ctcgactaac tacgtgttct
     4201 actgtttgtc ggttttagtt aaccagcagg ttgtcttaac gataatgtag ccgttatttt
     4261 taactaataa ggtcatattg ttaacgtcag gcataataaa agacattatg gcctcagagt
     4321 tatcatcaga agcagctctc tgcattacta cttcgattcc cttttctttg aatctataag
     4381 tatccagttg cctattaaac ttagaaagac ctacaaccat acttagtttg ttatctttaa
     4441 cagtaagaga tgcttcctga gtaccattac atcggtagta agcggtgtaa ccgtcagcaa
     4501 aagatcgacc ggggaatata aatgctagga acgcaattaa ccaatacata gttgagaatc
     4561 ttggtattac acatttcact atcctctctt tcatttttcg ttcaccatac tttgttatct
     4621 gatttttaat ttttctattt tataaaccaa gtctctacca aaaaggcgaa aattatcctc
     4681 aaataataat aattgtttaa gaacatccat gaaagcagct gcttcaccct cagttatggg
     4741 agctactcta tataacatat aacctgagtt aagggtttct tttatacttt cttcaatgct
     4801 cattataaat attagtggat tatcattttt cttcccccat gggtttctaa ttaatccttc
     4861 gatgtactca ggtcctttgc gtcctattgc ctgcgcaata ttttttattt catctgcgtg
     4921 agggctttgt tgtacagctt tgtttatgcc tgcttgagtg attttcagag actctacacc
     4981 gtgtatgttg tatgagataa aatgtaataa attaagatgc aatgcaatta atccattttt
     5041 gtttacatct gagtatttgg ataaaaaacg taatgaagtg ttaactctcc cttgaagcct
     5101 aacaattggg agtgctgcct tgtatatttc cttctccgtt aaataccgtt taaaaaacat
     5161 aattctccgc ctccgtatag cgtgcctatt ttatttcgtc gtaaaactta gctctgaaat
     5221 attcctttag aaccatcatg tcattaataa ttgaatctat ctcatccatt ttttcttggt
     5281 attcttttaa tggtgaaggg agcggtaact caatatttga aaagtgattg gcgagatagg
     5341 gatcaaggtt tctcatccat tcaggctttt cgccaatgcg ttcttcaact gaaagtgaac
     5401 ctgataatct ctggttaata agatcactta gactcatttt ggttttattc gaaagccgtt
     5461 gtaaggattg ctttaatcct atatctagca tgatatttgt tctttctctc attacccccc
     5521 cttgctattg cgttttttgt tgcgcacaaa gctaccattg cgcattaaga aacgcaataa
     5581 aaaaggaggg agggag
//
