LOCUS       NZ_MT312220             6027 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Escherichia coli isolate E566 plasmid pE566-OXA-232, complete
            sequence.
ACCESSION   NZ_MT312220
VERSION     NZ_MT312220.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Garcia,J.F., Nastro,M.B., Dabos,L., German,T., C,V.O., Hernan,R. and
            Carlos,V.
  TITLE     First report of Escherichia coli coproducing OXA-232 and mcr-1.1 in
            South America
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Garcia,J.F., Nastro,M.B., Dabos,L., German,T., C,V.O., Hernan,R. and
            Carlos,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2020) Bacteriologie, EA7361, Universite Paris Sud,
            Hopital Bicetre, 78 rue duGeneral Leclerc, Le Kremlin-bicetre 94275,
            Francia
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: CLC Workbench v. 12
            Assembly Name                     :: pE566-OXA232
            Coverage                          :: 70x
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/08/2024 02:00:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT312220.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="E566"
                     /isolation_source="abdominal collection"
                     /db_xref="taxon:562"
                     /plasmid="pE566-OXA-232"
                     /geo_loc_name="Argentina"
     gene            complement(236..1183)
                     /locus_tag="H5I66_RS00005"
     CDS             complement(236..1183)
                     /locus_tag="H5I66_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            <1559..1711
                     /locus_tag="H5I66_RS00010"
                     /pseudo=""
     CDS             <1559..1711
                     /locus_tag="H5I66_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            complement(2004..2801)
                     /locus_tag="H5I66_RS00015"
     CDS             complement(2004..2801)
                     /locus_tag="H5I66_RS00015"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            3145..3426
                     /locus_tag="H5I66_RS00020"
     CDS             3145..3426
                     /locus_tag="H5I66_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(3708..5048)
                     /locus_tag="H5I66_RS00050"
     CDS             complement(3708..5048)
                     /locus_tag="H5I66_RS00050"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_250911671.1"
                     /translation="MIEALIDSSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLP
                     GLEGNQYAVLWVEHRDKGRLELNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGE
                     LKLHDPDDPLNRQAVITPKDLPRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAG
                     FSVVRETKNSLSIADPDGGRNIRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIH
                     EARESYLRGVEIKRGENERRYCRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGG
                     IDSGAASHSPSLGREGRQAKADPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDG
                     IGAGIALRVRGFAERIRAAAQGMAGKLRELGEHEREHQTRGRDQQGSSRAIDEAGERLN
                     AASGRLEQAAGGLDRASQQVAKEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5143..5481)
                     /locus_tag="H5I66_RS00040"
     CDS             complement(5143..5481)
                     /locus_tag="H5I66_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5463..5855)
                     /locus_tag="H5I66_RS00045"
     CDS             complement(5463..5855)
                     /locus_tag="H5I66_RS00045"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     ARG             complement(2008..2801)
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(250..1171)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(3712..5162)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.347"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cattattcaa ccatgggcgg gatactgcaa gccctagagc aagatcaagg gaaagagcgg
       61 ctccgtggat aactgctgcc gcccacaccc cccgaaaaaa caggggggcg atggacggac
      121 aggcgcagtt gtccacctcg ccaaagatcc gcgctaaaag gattttttaa ggctaaaaga
      181 caagagcgca ggaacccccc taaagggggc tattatctga taaggcttgt ttcgatcacg
      241 gacgatcacg ctttaggtaa acgctcaccg tactggggga gatttgaaga tcgtccgcta
      301 tatcgcggtt ggtgtacccc tgagccttaa gccgcagaac ctccggcaat agatcggact
      361 tgtccttacc gctattgctc ggccttcccc ctcccttgga taccttgccg cccttcgcac
      421 ctctccgggc ctgctcctct ctgaaccctg cggggcttag gtgctggtgc gtccatttgg
      481 caatggactt ggccgtgtgc cggacttcgt ttgcggggag cggctgctcg aactgggcgt
      541 tatatcctga tgcccggtca acacaagcgg ccaaccattc ggggtaatcc ggccagccct
      601 gccggatcgc tttataggcc cacaggcgcg ttttctcgaa cagggtacag tttcgcccaa
      661 gcccgtactc aggaagcgat ttgcggccat tgtaggagct cagatcgagg taatcggata
      721 gccaggctaa atcgtagagc cggggttccc atacctgcac cagccagtgc tcatgcaggg
      781 ggttcttgca gatcagcccg gagtagccca tatccgcccc taatttctcc cggagcgcgg
      841 cctcgatggc ggcggcatac cgcagagggg ctgcatttcc atccggggca gtgcggatcg
      901 gcgtatccag tccgtagatc agatgcgcgt ggccgttggc cttgttcgtg acggtaatgt
      961 taggggctgg agcgcctacg tcatcccaag ataacgtagc ggctgcatgg tctacgtcaa
     1021 aaacgatcca gtggcggtgc gttggcccgt tcggctggat gtaacgcgca ccaagggcgc
     1081 gggcaacatc cgcaatccgt aggcccgttg taagctcgtc ggaatagtag ggtttccggg
     1141 gcaggcgctc ccgaaacagc tcaagctgtg cgcactttct caagcaccac cgcgcttatt
     1201 ggcggcgtgg ggttgtttgg catgatccat gtgatagact tctcttgtga ttcgtcacgg
     1261 cccccgtttt tcgcagctcc gccaagatgc aagcgataac gggggtttgt cttttctgga
     1321 caatacgtta cagcaggcac atcctagaga gcaacgttag ttgcatgtaa cctttcaccc
     1381 tggaacatat ttccacctcc ttctgcttct gcgctgtttt gaattgcttg atctacgcct
     1441 ctaactaggt cgatccttgg gattgagtgg caccattgtc agtgcaagaa tactgatcca
     1501 tataagtctc tctagtcgga caacaccaag tgttgaagag atcaaaaatc aacctatatc
     1561 ggaggattca tgtctgacat tgacagatgc ccccatgaaa gcaaagcgaa tgcggtccca
     1621 aagcgcctct gtagaaacga atttgagcga ggcatttgat gccatcgtct gcgtcccaag
     1681 cgccggcaag gacggcctgg ttgacctata gtaaaccggt agatgaaaat gatggagaac
     1741 caatatcagc gccttcttgg actttttcgt caaacgcttt agcagccaag aaagcctaca
     1801 atccttgatg ggctaggctt tttacgttta ggaatgtgat gctaatgctc aggctgaaag
     1861 ttaacgcgcg gagcttaagt cgattaggcc tgaccgatta agtatgagcc attaactaca
     1921 agcgcatcga gcatcagcat tttgtccata tatctttaca gcttatatga cggctatttc
     1981 acacaaatac gcgctaacca cttctaggga ataattttct cctgtttgag cacttctttt
     2041 gtgatggctt ggcgcagccc taaaccatcc gatgtgggca tatccatatt catcgcaaaa
     2101 aaccacacat tatcatcaag ttcaacccaa ccaacccacc agccaatctt aggttcgata
     2161 ctagtcgagt atcccgtttt agcccgaata atatagtcgc cattggcttc ggtcagcatg
     2221 gcttgtttca cgatgcgctg actacgctca gaaacgtgca gcttgttgtg atacagcttg
     2281 cgtaaaaaag cgatttgctg ggtagccgaa atgcgaatac caccatcgag ccaaaaactg
     2341 tctacattgc ccgagatatc ctcattgcca taatcgaagg cgtgcagcat tttactcata
     2401 cgtgcctcac caatttggcg ggcaaattct tgataaacag gcacaactga gtacttcatc
     2461 gcggtaatta agtcatggtc acgattccaa gcggcgatat cacgcgtctg tccatcccac
     2521 ttaaagactt ggtgttcatc cttaaccacg cccaaatcga gggcgatcaa gctattggga
     2581 attttaaagg tagatgcggg taaaaatgct tggttcgccc gtttaagatt attggtaaat
     2641 ccttgctgct tattctcatt ccagagcaca actacgccct gtgatttatg ttcagtaaag
     2701 tgagcattcc aacttttgtt ttcttgccat tcctttgcta ccgctggcat tccgataatc
     2761 gatgccacca aaaacacagc cgataaggct aatacacgca taacgtcccc ttgcttaatg
     2821 caatatatcc atacaaatta agtgctaagc ttatcattct ctgccctctc attccctagc
     2881 aaactgctga ccaattgtgg caacaatcag gcacaagctt taactattac ctaaattcca
     2941 cgtgtgtttt ttattagctt caaaaatcac tatttcacga agaatttaga ctgcttctca
     3001 cacattgtaa cattatttac aaccaccttt caatcatttt tgataaatca ttgatttcat
     3061 ctttgctgca atgatactta ataaactctg caagttatcc acagagcaac actcaatttt
     3121 attgatgata ttcttagggg gagtatggga ttttttaacc tatctaaagc gcttgaggag
     3181 gtgtctgatg cttttggggc taaagaaacc gcaatcgcta gtgccaagtt gttaggtaag
     3241 ggagttgtta acctagctca ttttgcagca acaacaggtt tggattcggt ggtcaaacaa
     3301 acgagcaaca aagttctcaa cagcgagtct tcaaccgaag aacaacgaga acaggctaaa
     3361 aagtctgccg catgggcaga cgcgagaatc gccgctagaa ataaagcaga aaacaccgaa
     3421 gactagctga acgcacattt cgacaatgat gtggactaca tcgttgatca taacttcacg
     3481 gtgcgctagc ttccatagaa gccaactaat aacaaggagt tagcatgtct gattcagatt
     3541 tcaaaaatag ccctgagtat gatgactgga ttgaatctgg tggtagagat gaagattaca
     3601 gttactacta tgaaaaatgg catagaagaa ctcaacgata attcccgccg ataaaaggaa
     3661 agtgatagca ctttcctttt tttataactg acggccgttt tgttccgtca acgctcaaag
     3721 cctcgcgatc tgtctccacg ctcccagcct cgatccagtg ccttcaggag cctgtgttcc
     3781 ctttcttttg caacttgctg actagcccgg tcaagccctc cagctgcttg ctcaagccgt
     3841 ccacttgctg cgttaagccg ttcacctgcc tcgtcaatcg ctctgctgct gccctgctga
     3901 tctcggcctc ttgtttggtg ctctcgttca tgctcgccaa gttcgcgtag ctttcctgcc
     3961 atgccttgtg ctgctgctcg tatgcgctct gcaaagcctc gaacgcgcag agcaattccc
     4021 gctccaatcc cgtcatttag taccccttcg gcacgatcca ataggtatcc ttcccccaaa
     4081 cggttggatt ggggtcggag atctgcacgc aaaggtgctc caatttcttc ccgcactggg
     4141 tcagctttag cttgccgccc ttctctgcca aggcttggag agtggcttgc cgctccgcta
     4201 tcaattcccc ctgataccag aggatcccgc tcgttgcgct caagaacagg acgatgctga
     4261 ccagcatcca gatccacgtt ttgagcaaca gccagcccat tttctggctg tgctcggcaa
     4321 tagcggcgct cattttctcc tcgcttgatt tcaacgcccc ggagatagct ttctcgtgcc
     4381 tcgtgaattc ttcggtcaga cgcttctcga tagcgttggc ctgctgcttc gagctctgct
     4441 cgaagtcctt cgccatgtct aaaatcttgc tcatacagca ccccctttaa tcggatgttc
     4501 cgccccccgt ccgggtcggc aatgctcagg ctgtttttgg tttcgcgtac cacgctgaaa
     4561 cccgcaccct gcaaggcttt gcgcacatca tcgcggctct ggatctcccc tgctccggcc
     4621 atgcgtaaaa gcccgtctgt aatggcctga gcggcctcct tgtgccccct cggcaaatcc
     4681 tttggcgtga taacggcctg acggtttaac ggatcgtccg ggtcgtgcaa tttcagctcc
     4741 ccgtttacca cggttcgcca cgcatcgagg cgcggattgt cggccttgtg gaagtagggt
     4801 tgcagccgct tcccgctctg caattcgatg ttcgggacta caaaattcag ctccagccgc
     4861 cccttgtcgc ggtgctctac ccacaaaaca gcgtactggt tgccctccag tccgggcagg
     4921 agcgcctttt caaagtcgct catcagcttg tccttggtcg ccctgggtaa atcagcctcg
     4981 gcaaacgaca gaacgccaga ggtgtatttt ttggcgtagg gactggaatc aatcagggct
     5041 tcaatcaccg cagggttgcc ccggtcgagc gttgcgcctt cccggttccc gtcacggccc
     5101 aacaagtagc caacaggccc ggagccccgg cctaccccac ggttatggaa tttgatgatc
     5161 atggtgcagt tcctccagct cgcggcctat agcggacagc tccgctatga tgcgcagccg
     5221 atccaacggc ccccactccc cggaattaac gcgccgggcg atctggttca ggttgttgcc
     5281 cattccggcc aactgccgca agagcgccgg atctacggta ggcgccggcg ttcggcgctg
     5341 gggttgcgca acaccgagac attgctcccg catccactcc gccagttggg ccttggggca
     5401 gagctcccgc aggcgctcaa gctccgcatc tgagacccgg atcttgatag agcggaggcg
     5461 ctttaacggc tggccagcca tgcagaggcc tccgctaagg cgtcctcata gaggctatcg
     5521 agtagcgtct ggagcgcctt ttcatcccga aaatcgcagt gctccggcaa ggggaaaacc
     5581 cccccgccga cctcttcgcc gttctccagc aaacgcagat tccagccccc gccaagaccg
     5641 agagtgcggg gggtgatttc gtagtcgtat tccgttgttg tgggttcgtc acttaccata
     5701 gctaacacct catcggtgga aatttttcca ttgatataat cttcaagtag aattttgaat
     5761 tcatcggtta tggcctcccc ctctagttgg aaactagaaa taacgccatt gactgccttc
     5821 cttctcgaat ctttctctat ctgtgttgtt gacatagggc ctcgtccatt ctccagagcc
     5881 aagaagccac cgcgcagacc gtcccgaagg ggcggatcga gcgggggcca cagggggttt
     5941 cgggggggcg tagcacccct gaacaaggcc acagaggccc gagaccgcga agcaggtaga
     6001 ggggcctctg tgtagccttg cttagta
//
