LOCUS       NZ_MT180435             6075 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Escherichia coli strain CE1681 plasmid pCE1681-F, complete sequence.
ACCESSION   NZ_MT180435
VERSION     NZ_MT180435.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Tarabai,H., Wyrsch,E.R., Bitar,I., Djordjevic,S.P. and Dolejska,M.
  TITLE     Comparative analysis of multidrug resistant Escherichia coli ST216
            isolates from silver gulls in Australia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Tarabai,H., Wyrsch,E.R., Bitar,I., Djordjevic,S.P. and Dolejska,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2020) Department of Biology and Wildlife Diseases,
            University of Veterinary and Pharmaceutical Sciences Brno, Palackeho
            tr. 1946/1, Brno, Brno 61242, Czech Republic
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: SMRT Link v. 8.0
            Assembly Name                :: pCE1681-F
            Coverage                     :: 326X
            Sequencing Technology        :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/08/2024 01:59:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT180435.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CE1681"
                     /host="Chroicocephalus novaehollandiae"
                     /db_xref="taxon:562"
                     /plasmid="pCE1681-F"
                     /geo_loc_name="Australia"
                     /collection_date="2012"
                     /type="ST216"
                     /note="ColE plasmid"
     gene            complement(join(5681..6075,1..139))
                     /locus_tag="IFO76_RS00005"
     CDS             complement(join(5681..6075,1..139))
                     /locus_tag="IFO76_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031944069.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(142..1068)
                     /locus_tag="IFO76_RS00010"
     CDS             complement(142..1068)
                     /locus_tag="IFO76_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_023148976.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1245..1643
                     /locus_tag="IFO76_RS00015"
     CDS             1245..1643
                     /locus_tag="IFO76_RS00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560793.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(1847..2761)
                     /locus_tag="IFO76_RS00020"
     CDS             complement(1847..2761)
                     /locus_tag="IFO76_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032333168.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(3260..3403)
                     /locus_tag="IFO76_RS00025"
     CDS             complement(3260..3403)
                     /locus_tag="IFO76_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001560790.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(3465..3728)
                     /locus_tag="IFO76_RS00030"
     CDS             complement(3465..3728)
                     /locus_tag="IFO76_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_001560791.1"
                     /translation="MELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKIT
                     EHPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(3730..5460)
                     /locus_tag="IFO76_RS00035"
     CDS             complement(3730..5460)
                     /locus_tag="IFO76_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_048266662.1"
                     /translation="MSGGDGRGHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGHGNGGGNSNSGGGSNSSVAAPMAFGFPALAAPGAGTLGISVSG
                     EALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQ
                     VSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGV
                     PSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVL
                     VTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARNQERQA
                     KAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDV
                     NNKKAAFDAAAKEKSDADVALSSALERRKQKENKEKDAKAKLDKESKRNKPGKATGKGK
                     PVNNKWLNNAGKDLGSPVPDRIANKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFSRNN
                     NDRMKVGKAPKTRTQDVSGKRTSFELHHEKPISQNGGVYDMDNISVVTPKRHIDIHRGK
                     "
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             1..6075
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1987..2761)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.139"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(146..1068)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.104"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatatcccg atatccgcga agtgtttccc ttgccacagc cagagaatca gcaacagctt
       61 tctgttgctc cattgcggta tgttgcagat gctccaccga taacaggagc gtgagtaatt
      121 tttcttctgt gctggccatg ttcagaatcc catagaaaat ccacgtgacg gtgatttaac
      181 ccgttcaggt tcagttcggt cctgtctgct cactctgtct ctcatgtcaa agccatgctc
      241 ccgcgcaaca tcatgttgaa gccttctgac cagttcatgt tgcttaaccg gagtgtccag
      301 ccattccctg atgtcatgcc cgaccgttcg cgcctgttca agatggcggg aatagtccgg
      361 ttcagaccgt ttttgcatga tgtcatgacg ctgggcatca tcaagccgtc cggcagcttc
      421 aaaacccaga tgcacttcgg gacgtcgttc aatgccctgt tctttcagcg tccggcagtc
      481 aatgaagccc ctttcaaagc catgctttct catatgttcg ttgtgcttca cctcccagcg
      541 ttttcgcaat tcccgcaact gctctttttg ctgagtgaga gtcaggcttc cggaatcctt
      601 tttcgcacct ccccgctcgg gatacctggc gttatagcgc cggaaatact gttccggtgt
      661 acggtcaatg ccatcattca cacgctggct catcatgata tgcgcatgag gctgttcgcc
      721 gccgtcaatg ctcgctttcg gattgtgaat ggcaaaactc caggcgtgcc gttcgccggc
      781 ttcctgacgt acaaaatcct gcaccagttc aaggcgctgt tccggtgtca gttcacgggg
      841 cagcgcgatt tccagttccc gataagtcga accgttcgca cgttcaaact ggtcagccgc
      901 ctgccagaaa tgggaaggat tatccctggc ccattccggc atgtttccgt gctccgtgta
      961 ttccagatct ttccgtttcg catactcccc tttgcgctcg atgtaatcgg cgtggtttgc
     1021 agcctgtccc ttgcctccaa atttgacgct gaggtgatag gttgccacgg tttcagccct
     1081 ccgcaggagc gcactgtttc tcgacgaagg agagaaacat ataagtgcgc ttccttctaa
     1141 ttttacgaat tagcacggaa gatacccccc tgcacccacc gaccgcaagc gatatactgc
     1201 acgaccacaa cacaccgcaa aaaacaccag ggagcaccac gaacatgcgc atcagcaaca
     1261 tcgaatggct gaagaaacgg atcggattca tcaggaagct cggagaacag accgcacgac
     1321 agagacagat tattgacctg ctcgacaacg aagcaggact cacagaacag gaacgcaaac
     1381 tcctccacgt actggcaaca gcggaaaaaa acgacttaca ggcacaggag agcgaacgaa
     1441 aacaggcggt ccaaaaacga atcgagggaa aaaaacaacg acgggaacgc aatcaccggc
     1501 tctttctcgc ggctgggcta ctgatcgaag caggactggt agacacaaaa acaggcgagc
     1561 tatgctataa aaaagacagg atattacagg cacttaagga acttaagtac gatctggaga
     1621 catccccaaa cccagacgcg taagtgcctc catccgtgtt ccgattccct ggtgcttcct
     1681 cgctcattcg ctcgctacgc tcggtcatga gactgcggcg agcactacca gcgcaatcat
     1741 gcggaaaaca gcaccattgc agggctgaac ggggaaaaac ggcaaaaaac ctgctgctca
     1801 aaaagacgcc aaaaaggcgc tgttatctga tataggctta tttggtctac tctacgagac
     1861 ctcgtttttt cagttggtaa taccaggcgc gactgatccc taatgcctcc cacggtttca
     1921 gcgtgcgtgc agatgtagcc actgtgctgc gcttagactt agaaccgccc ttgcgaccgc
     1981 gtgcagcctg aatttctggt gtgtgcgtat cagccacata ctgtgcgaaa gtttccggcg
     2041 tgaagttcct gtgcgtgtat ttcgcaatgc tcctgccaat agcccgacat tcaggcggtg
     2101 aaagcggaac gggaagcgat gcgttgtaca tttcgacacg ctgaatcacg gcatcaagcc
     2161 actgtgagaa ttcaggccag ccctgacgaa tcgccctgta agcccatttg cgcgtcattt
     2221 cgaacaggtg gcagtttcgc cccatcccgt aatgtttatc gatgctacgg cgcgctgagg
     2281 cgctcaaatc gagataatca gccagttcat cgagggtata ggcttcctct cgccactcca
     2341 tcacctgcca ttccaggtgg aacggatttt tacaaatcag tccgctgtag ttcacatccg
     2401 cacaaagttt ctcacacagc gaacgttcaa tcgcagcggc gtatttcagc gccttaaccg
     2461 agctgtccgg cgcggttctc acggcgatat tgagcgcgta gagcaggtga gcatgaccgt
     2521 tcacaggatt ttttacggtg atgttcggcg cgggagcgtt ccggtcgctc cagtcaatcg
     2581 ctgccccctc gcggtcaacg tcaaaaacca gccagaactg tgcatgaggc tggttctgct
     2641 gtatgtaccg cgcaagaagc gcacgccctt tgccggagat gcgaagacca aaagcgagat
     2701 catccgtgtg atagggacgg tggggtaaat tttcgtcgaa gtattgaagc gcggcgctca
     2761 tgtgttacga ttctctccgg gttatgcgac ctacacgccc tctgtatttc atgttgaatc
     2821 ttgccggatc tggacatgat ttcaggggcg tttgtttttc agtcgcttac actacaccca
     2881 acggcaaatc ctgtcgaacc ttcccgaaaa cccgatccat ttaccataac tcccgttaaa
     2941 cgcaccgcta aaacgcgctc aatcgctttc tgacgcaaaa aacgcaaaaa gacaaggaaa
     3001 caccaggctg aaccagaaaa atgcgtcaga atcgttttta ggggcctgca taactatgca
     3061 cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca aaacgaaaag
     3121 ttttctccct cctgatggaa ggttctgcac ggagatttta gatcccatct cccacatgga
     3181 atttttgtgg cacttcaaca aaaaaagcgc gtcagaatca gcctgacacg ctttgttctt
     3241 gtcaaatgag gattgcaggt tactgcgttt ccactccggt cagttcagca gttgacgacg
     3301 gtgatactgt cccgccctga acatcacgga tatagtttgc ctgacatgca gcaagaataa
     3361 tggctgcaag aagcaataaa ataatccctg ttattttttt cataacgact ccttgttgtg
     3421 aaagaatgcc agtcattcat ccttaaacaa ccggcattca tattttagcc ctgtttaaat
     3481 cctggcttac cgttagcagc tcgccattct ttaatttcct tgacaatccc ttcggggcta
     3541 tcgtctctat tatcactagg ataataaatc agatccgttc catctggatg ctcagtaatt
     3601 tttacaaagt gttcgagtaa cacatctaac acatcatcag ttgcagcaac attctctttt
     3661 tcaatttcct taagaagttg aacaaactca gcctctgtgt aatcactaat actatttttc
     3721 agttccatat tatttacctc ggtgaatatc aatatgcctt ttaggtgtta ccacgctgat
     3781 gttatccata tcatagacac caccattttg gctgatcggc ttctcatgat gaagctcgaa
     3841 tgaagttctc ttccctgaaa catcctgggt tctagtcttg ggcgcttttc caaccttcat
     3901 tcgatcatta ttgttcctac taaattgttt acttaactca ggatcttttg acacttcttc
     3961 ccagaatttc ttacgaaaat catcgaaact tttaaactcc ttatcacgta gtttattagc
     4021 tatacgatct ggaacaggag aacctaagtc tttacctgca ttatttaacc acttattatt
     4081 gacaggtttt cctttacctg ttgccttccc tggcttattc cgtttactct ccttatctaa
     4141 tttggcttta gcgtcctttt ctttattttc tttctgtttg cggcgctcca acgcagaact
     4201 caatgcaaca tcagcatctg acttttcttt tgcagcagca tcaaatgcag ccttcttatt
     4261 atttacatcc gtctgtgccc gctgggcctt aagccctgcc atttgccaca ttctgtgacc
     4321 agcagccatt ggttctcggg caaatcgctc gaattgtttt atttcagcct ttgcatcagc
     4381 aagagtttta ttcgctgcat caagttcact tttacgagaa ttataaacct gaacagcttt
     4441 agcctgtcgt tcctggtttc tggcaacatc tttattagcc tgattcagtt ctgcgcgggc
     4501 ctgttcataa ttgcgttcag ccacttccac cggatgtgcg tcattccatt cctgctggag
     4561 gcgtttttcc tcatcctgac gttgttttac ctgtgctggg gtaagaacat ctgtcactaa
     4621 tacataaacc ggcttctgcc cactttcttt cggaaagcga atcaccgctt catgtgaacc
     4681 accaccaaag gtaaatcccg caggaacggc agtccggcct ttatcctccg taataccacg
     4741 gggaagggtt gttgacaccg gaaggccttt gacagtgctg actgtcagag aagggacacc
     4801 aggaaatgat gcgtgaaata cacctggagt acgtgttggt ttggcattca caacaggcac
     4861 actcataggc acacctgcaa ctacggcaat atgctgtcgt gtgtccttca caacatctgt
     4921 cacacgtttc gtcacgctga cagttgcctg gtccagcgga agtgtactga cctgaacatt
     4981 cgtcactgtt tctgccggta atgacgtcac aatctttgac atcatattcg ggtcatcttt
     5041 tgctatttca gatggcagaa tgccgtaaag cgcaataccc catgcgctga atttaaacgg
     5101 acctttcagg gctgcaaaga tatccgctat tgccgcagat aaagcctcac cagatacgga
     5161 aataccaaga gttccggcac caggagcagc caaagcagga aaaccaaatg ccataggtgc
     5221 tgctacggat gaattgctgc caccaccgga attactattc cccccgccat tgccatggcc
     5281 ggagccacct ccccagtgaa caccactacc ggaaccgcca ccccatgggt tattttccga
     5341 actccatccg gagccgtcag aagccccacc atttccacca agccccgtag ggccgccatt
     5401 aatgttacca cctgtgttat gtgcgccact gttgtggcca cgtccatctc caccgctcat
     5461 aaaaattccc tcttttaagc gttaaaacaa ttaattacat agttaatact gtacataaaa
     5521 ccactggttt tatgtacaga tcaaaaagta ctcgccatta ttttctctgt caagtcaggg
     5581 agggatattt tttggcagtt cggaggagtc actcatggaa acggtgaacg gttcgcgaaa
     5641 tcctttcgcg gtccgtgagg cgttggacaa acgaagcgcg tcagtttttc cggctcttac
     5701 ccgtgacagg tttctccgcc agtttatctt ccagtgtcac aactttctga tacaccgccg
     5761 ctgtggcgtt gtagttcctg tcgagaattg ttttcatccc ccatccgagc acactgaaca
     5821 taaccagaaa ggccactgac accccaccca gccatttcca gctataccct ttaagggtgt
     5881 tgtattcctg cacgatattg ctgtgaattg tcgcaatatc cctgttcagg gcatccgcgt
     5941 ttgccttcat gtttgcgact gactcttcca gccgctgaaa atcctgactg atgcgctcac
     6001 ctatcagccc tgcaacatcc agcctctgca cttcgtttct gacttcatcc ctgacctgtt
     6061 ttgccacacc gtcag
//
