LOCUS       NZ_MT180432            11153 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Escherichia coli strain CE1681 plasmid pCE1681-C, complete sequence.
ACCESSION   NZ_MT180432
VERSION     NZ_MT180432.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Tarabai,H., Wyrsch,E.R., Bitar,I., Djordjevic,S.P. and Dolejska,M.
  TITLE     Comparative analysis of multidrug resistant Escherichia coli ST216
            isolates from silver gulls in Australia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11153)
  AUTHORS   Tarabai,H., Wyrsch,E.R., Bitar,I., Djordjevic,S.P. and Dolejska,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2020) Department of Biology and Wildlife Diseases,
            University of Veterinary and Pharmaceutical Sciences Brno, Palackeho
            tr. 1946/1, Brno, Brno 61242, Czech Republic
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: SMRT Link v. 8.0
            Assembly Name                     :: pCE1681-C
            Coverage                          :: 326X
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/08/2024 02:02:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 13
            CDSs (total)                      :: 13
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 2 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT180432.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CE1681"
                     /host="Chroicocephalus novaehollandiae"
                     /db_xref="taxon:562"
                     /plasmid="pCE1681-C"
                     /geo_loc_name="Australia"
                     /collection_date="2012"
                     /type="ST216"
                     /note="Col156 plasmid"
     gene            <1..534
                     /locus_tag="IFO77_RS00005"
                     /pseudo=""
     CDS             <1..534
                     /locus_tag="IFO77_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            537..1070
                     /locus_tag="IFO77_RS00010"
     CDS             537..1070
                     /locus_tag="IFO77_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031944069.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            1291..3021
                     /locus_tag="IFO77_RS00015"
     CDS             1291..3021
                     /locus_tag="IFO77_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_048266662.1"
                     /translation="MSGGDGRGHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGHGNGGGNSNSGGGSNSSVAAPMAFGFPALAAPGAGTLGISVSG
                     EALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQ
                     VSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGV
                     PSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVL
                     VTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARNQERQA
                     KAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDV
                     NNKKAAFDAAAKEKSDADVALSSALERRKQKENKEKDAKAKLDKESKRNKPGKATGKGK
                     PVNNKWLNNAGKDLGSPVPDRIANKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFSRNN
                     NDRMKVGKAPKTRTQDVSGKRTSFELHHEKPISQNGGVYDMDNISVVTPKRHIDIHRGK
                     "
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3023..3286
                     /locus_tag="IFO77_RS00020"
     CDS             3023..3286
                     /locus_tag="IFO77_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_001560791.1"
                     /translation="MELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKIT
                     EHPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            3348..3491
                     /locus_tag="IFO77_RS00025"
     CDS             3348..3491
                     /locus_tag="IFO77_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001560790.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     gene            3990..4904
                     /locus_tag="IFO77_RS00030"
     CDS             3990..4904
                     /locus_tag="IFO77_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032333168.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(5181..5507)
                     /locus_tag="IFO77_RS00035"
     CDS             complement(5181..5507)
                     /locus_tag="IFO77_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_190259823.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKKRLTGTGERTKTGGPKTNRGKKTTTGTQSPALSRGWATDRSRTGRHKNR
                     RAML"
                     /name="hypothetical protein"
     gene            5684..6610
                     /locus_tag="IFO77_RS00040"
     CDS             5684..6610
                     /locus_tag="IFO77_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_023148976.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            6613..7146
                     /locus_tag="IFO77_RS00045"
     CDS             6613..7146
                     /locus_tag="IFO77_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031944069.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(7461..7994)
                     /locus_tag="IFO77_RS00050"
     CDS             complement(7461..7994)
                     /locus_tag="IFO77_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031944069.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(7997..8923)
                     /locus_tag="IFO77_RS00055"
     CDS             complement(7997..8923)
                     /locus_tag="IFO77_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_023148976.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            9100..9426
                     /locus_tag="IFO77_RS00060"
     CDS             9100..9426
                     /locus_tag="IFO77_RS00060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_190259823.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKKRLTGTGERTKTGGPKTNRGKKTTTGTQSPALSRGWATDRSRTGRHKNR
                     RAML"
                     /name="hypothetical protein"
     gene            complement(9708..10620)
                     /locus_tag="IFO77_RS00065"
                     /pseudo=""
     CDS             complement(9708..10620)
                     /locus_tag="IFO77_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     BGC             1..8021
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3991..4765
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.139"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(9845..10620)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.015"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          31..305
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.739"
                     /coverage="100.0"
                     /evalue="1.57e-50"
     Typing          5685..6607
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.104"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8001..8923)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.104"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatggcattg accgtacacc ggaacagtat tccggcgcta taacgccagg tatcccgagc
       61 ggggaggtgc gaaaaaggat tccggaagcc tgactctcac tcagcaaaaa gagcagttgc
      121 gggaattgcg aaaacgctgg gaggtgaagc acaacgaaca tatgagaaag catggctttg
      181 aaaggggctt cattgactgc cggacgctga aagaacaggg cattgaacga cgtcccgaag
      241 tgcatctggg ttttgaagct gccggacggc ttgatgatgc ccagcgtcat gacatcatgc
      301 aaaaaacggt ctgaaccgga ctattcccgc catcttgaac aggcgcgaac ggtcgggcat
      361 gacatcaggg aatggctgga cactccggtt aagcaacatg aactggtcag aaggcttcaa
      421 catgatgttg cgcgggagca tggctttgac atgagagaca gagtgagcag acaggaccga
      481 actgaacctg aacgggttaa atcaccgtca cgtggatttt ctatgggatt ctgaacatgg
      541 ccagcacaga agaaaaatta ctcacgctcc tgttatcggt ggagcatctg caacataccg
      601 caatggagca acagaaagct gttgctgatt ctctggctgt ggcaagggaa acacttcgcg
      661 gatatcggga tatttctgac ggtgtggcaa aacaggtcag ggatgaagtc agaaacgaag
      721 tgcagaggct ggatgttgca gggctgatag gtgagcgcat cagtcaggat tttcagcggc
      781 tggaagagtc agtcgcaaac atgaaggcaa acgcggatgc cctgaacagg gatattgcga
      841 caattcacag caatatcgtg caggaataca acacccttaa agggtatagc tggaaatggc
      901 tgggtggggt gtcagtggcc tttctggtta tgttcagtgt gctcggatgg gggatgaaaa
      961 caattctcga caggaactac aacgccacag cggcggtgta tcagaaagtt gtgacactgg
     1021 aagataaact ggcggagaaa cctgtcacgg gtaagagccg gaaaaactga cgcgcttcgt
     1081 ttgtccaacg cctcacggac cgcgaaagga tttcgcgaac cgttcaccgt ttccatgagt
     1141 gactcctccg aactgccaaa aaatatccct ccctgacttg acagagaaaa taatggcgag
     1201 tactttttga tctgtacata aaaccagtgg ttttatgtac agtattaact atgtaattaa
     1261 ttgttttaac gcttaaaaga gggaattttt atgagcggtg gagatggacg tggccacaac
     1321 agtggcgcac ataacacagg tggtaacatt aatggcggcc ctacggggct tggtggaaat
     1381 ggtggggctt ctgacggctc cggatggagt tcggaaaata acccatgggg tggcggttcc
     1441 ggtagtggtg ttcactgggg aggtggctcc ggccatggca atggcggggg gaatagtaat
     1501 tccggtggtg gcagcaattc atccgtagca gcacctatgg catttggttt tcctgctttg
     1561 gctgctcctg gtgccggaac tcttggtatt tccgtatctg gtgaggcttt atctgcggca
     1621 atagcggata tctttgcagc cctgaaaggt ccgtttaaat tcagcgcatg gggtattgcg
     1681 ctttacggca ttctgccatc tgaaatagca aaagatgacc cgaatatgat gtcaaagatt
     1741 gtgacgtcat taccggcaga aacagtgacg aatgttcagg tcagtacact tccgctggac
     1801 caggcaactg tcagcgtgac gaaacgtgtg acagatgttg tgaaggacac acgacagcat
     1861 attgccgtag ttgcaggtgt gcctatgagt gtgcctgttg tgaatgccaa accaacacgt
     1921 actccaggtg tatttcacgc atcatttcct ggtgtccctt ctctgacagt cagcactgtc
     1981 aaaggccttc cggtgtcaac aacccttccc cgtggtatta cggaggataa aggccggact
     2041 gccgttcctg cgggatttac ctttggtggt ggttcacatg aagcggtgat tcgctttccg
     2101 aaagaaagtg ggcagaagcc ggtttatgta ttagtgacag atgttcttac cccagcacag
     2161 gtaaaacaac gtcaggatga ggaaaaacgc ctccagcagg aatggaatga cgcacatccg
     2221 gtggaagtgg ctgaacgcaa ttatgaacag gcccgcgcag aactgaatca ggctaataaa
     2281 gatgttgcca gaaaccagga acgacaggct aaagctgttc aggtttataa ttctcgtaaa
     2341 agtgaacttg atgcagcgaa taaaactctt gctgatgcaa aggctgaaat aaaacaattc
     2401 gagcgatttg cccgagaacc aatggctgct ggtcacagaa tgtggcaaat ggcagggctt
     2461 aaggcccagc gggcacagac ggatgtaaat aataagaagg ctgcatttga tgctgctgca
     2521 aaagaaaagt cagatgctga tgttgcattg agttctgcgt tggagcgccg caaacagaaa
     2581 gaaaataaag aaaaggacgc taaagccaaa ttagataagg agagtaaacg gaataagcca
     2641 gggaaggcaa caggtaaagg aaaacctgtc aataataagt ggttaaataa tgcaggtaaa
     2701 gacttaggtt ctcctgttcc agatcgtata gctaataaac tacgtgataa ggagtttaaa
     2761 agtttcgatg attttcgtaa gaaattctgg gaagaagtgt caaaagatcc tgagttaagt
     2821 aaacaattta gtaggaacaa taatgatcga atgaaggttg gaaaagcgcc caagactaga
     2881 acccaggatg tttcagggaa gagaacttca ttcgagcttc atcatgagaa gccgatcagc
     2941 caaaatggtg gtgtctatga tatggataac atcagcgtgg taacacctaa aaggcatatt
     3001 gatattcacc gaggtaaata atatggaact gaaaaatagt attagtgatt acacagaggc
     3061 tgagtttgtt caacttctta aggaaattga aaaagagaat gttgctgcaa ctgatgatgt
     3121 gttagatgtg ttactcgaac actttgtaaa aattactgag catccagatg gaacggatct
     3181 gatttattat cctagtgata atagagacga tagccccgaa gggattgtca aggaaattaa
     3241 agaatggcga gctgctaacg gtaagccagg atttaaacag ggctaaaata tgaatgccgg
     3301 ttgtttaagg atgaatgact ggcattcttt cacaacaagg agtcgttatg aaaaaaataa
     3361 cagggattat tttattgctt cttgcagcca ttattcttgc tgcatgtcag gcaaactata
     3421 tccgtgatgt tcagggcggg acagtatcac cgtcgtcaac tgctgaactg accggagtgg
     3481 aaacgcagta acctgcaatc ctcatttgac aagaacaaag cgtgtcaggc tgattctgac
     3541 gcgctttttt tgttgaagtg ccacaaaaat tccatgtggg agatgggatc taaaatctcc
     3601 gtgcagaacc ttccatcagg agggagaaaa cttttcgttt tgagccgttc ggtgttcaga
     3661 acgcacgaaa ccgatcgcac atcgctttcg tgcatagtta tgcaggcccc taaaaacgat
     3721 tctgacgcat ttttctggtt cagcctggtg tttccttgtc tttttgcgtt ttttgcgtca
     3781 gaaagcgatt gagcgcgttt tagcggtgcg tttaacggga gttatggtaa atggatcggg
     3841 ttttcgggaa ggttcgacag gatttgccgt tgggtgtagt gtaagcgact gaaaaacaaa
     3901 cgcccctgaa atcatgtcca gatccggcaa gattcaacat gaaatacaga gggcgtgtag
     3961 gtcgcataac ccggagagaa tcgtaacaca tgagcgccgc gcttcaatac ttcgacgaaa
     4021 atttacccca ccgtccctat cacacggatg atctcgcttt tggtcttcgc atctccggca
     4081 aagggcgtgc gcttcttgcg cggtacatac agcagaacca gcctcatgca cagttctggc
     4141 tggtttttga cgttgaccgc gagggggcag cgattgactg gagcgaccgg aacgctcccg
     4201 cgccgaacat caccgtaaaa aatcctgtga acggtcatgc tcacctgctc tacgcgctca
     4261 atatcgccgt gagaaccgcg ccggacagct cggttaaggc gctgaaatac gccgctgcga
     4321 ttgaacgttc gctgtgtgag aaactttgtg cggatgtgaa ctacagcgga ctgatttgta
     4381 aaaatccgtt ccacctggaa tggcaggtga tggagtggcg agaggaagcc tataccctcg
     4441 atgaactggc tgattatctc gatttgagcg cctcagcgcg ccgtagcatc gataaacatt
     4501 acgggatggg gcgaaactgc cacctgttcg aaatgacgcg caaatgggct tacagggcga
     4561 ttcgtcaggg ctggcctgaa ttctcacagt ggcttgatgc cgtgattcag cgtgtcgaaa
     4621 tgtacaacgc atcgcttccc gttccgcttt caccgcctga atgtcgggct attggcagga
     4681 gcattgcgaa atacacgcac aggaacttca cgccggaaac tttcgcacag tatgtggctg
     4741 atacgcacac accagaaatt caggctgcac gcggtcgcaa gggcggttct aagtctaagc
     4801 gcagcacagt ggctacatct gcacgcacgc tgaaaccgtg ggaggcatta gggatcagtc
     4861 gcgcctggta ttaccaactg aaaaaacgag gtctcgtaga gtagaccaaa taagcctata
     4921 tcagataaca gcgccttttt ggcgtctttt tgagcagcag gttttttgcc gtttttcccc
     4981 gttcagccct gcaatggtgc tgttttccgc atgattgcgc tggtagtgct cgccgcagtc
     5041 tcatgaccga gcgtagcgag cgaatgagcg aggaagcacc agggaatcgg aacacggatg
     5101 gaggcactta cgcgtctggg tttggggatg tctccagatc gtacttaagt tccttaagtg
     5161 cctgtaatat cctgtctttt ttatagcata gctcgcctgt ttttgtgtct accagtcctg
     5221 cttcgatcag tagcccagcc gcgagaaaga gccggtgatt gcgttcccgt cgttgttttt
     5281 ttccctcgat tcgtttttgg accgcctgtt ttcgttcgct ctcctgtgcc tgtaagtcgt
     5341 tttttttccg ctgttgccag tacgtggagg agtttgcgtt cctgttctgt gagtcctgct
     5401 tcgttgtcga gcaggtcaat aatctgtctc tgtcgtgcgg tctgttctcc gagcttcctg
     5461 atgaatccga tccgtttctt cagccattcg atgttgctga tgcgcatgtt cgtggtgctc
     5521 cctggtgttt tttgcggtgt gttgtggtcg tgcagtatat cgcttgcggt cggtgggtgc
     5581 aggggggtat cttccgtgct aattcgtaaa attagaagga agcgcactta tatgtttctc
     5641 tccttcgtcg agaaacagtg cgctcctgcg gagggctgaa accgtggcaa cctatcacct
     5701 cagcgtcaaa tttggaggca agggacaggc tgcaaaccac gccgattaca tcgagcgcaa
     5761 aggggagtat gcgaaacgga aagatctgga atacacggag cacggaaaca tgccggaatg
     5821 ggccagggat aatccttccc atttctggca ggcggctgac cagtttgaac gtgcgaacgg
     5881 ttcgacttat cgggaactgg aaatcgcgct gccccgtgaa ctgacaccgg aacagcgcct
     5941 tgaactggtg caggattttg tacgtcagga agccggcgaa cggcacgcct ggagttttgc
     6001 cattcacaat ccgaaagcga gcattgacgg cggcgaacag cctcatgcgc atatcatgat
     6061 gagccagcgt gtgaatgatg gcattgaccg tacaccggaa cagtatttcc ggcgctataa
     6121 cgccaggtat cccgagcggg gaggtgcgaa aaaggattcc ggaagcctga ctctcactca
     6181 gcaaaaagag cagttgcggg aattgcgaaa acgctgggag gtgaagcaca acgaacatat
     6241 gagaaagcat ggctttgaaa ggggcttcat tgactgccgg acgctgaaag aacagggcat
     6301 tgaacgacgt cccgaagtgc atctgggttt tgaagctgcc ggacggcttg atgatgccca
     6361 gcgtcatgac atcatgcaaa aacggtctga accggactat tcccgccatc ttgaacaggc
     6421 gcgaacggtc gggcatgaca tcagggaatg gctggacact ccggttaagc aacatgaact
     6481 ggtcagaagg cttcaacatg atgttgcgcg ggagcatggc tttgacatga gagacagagt
     6541 gagcagacag gaccgaactg aacctgaacg ggttaaatca ccgtcacgtg gattttctat
     6601 gggattctga acatggccag cacagaagaa aaattactca cgctcctgtt atcggtggag
     6661 catctgcaac ataccgcaat ggagcaacag aaagctgttg ctgattctct ggctgtggca
     6721 agggaaacac ttcgcggata tcgggatatt tctgacggtg tggcaaaaca ggtcagggat
     6781 gaagtcagaa acgaagtgca gaggctggat gttgcagggc tgataggtga gcgcatcagt
     6841 caggattttc agcggctgga agagtcagtc gcaaacatga aggcaaacgc ggatgccctg
     6901 aacagggata ttgcgacaat tcacagcaat atcgtgcagg aatacaacac ccttaaaggg
     6961 tatagctgga aatggctggg tggggtgtca gtggcctttc tggttatgtt cagtgtgctc
     7021 ggatggggga tgaaaacaat tctcgacagg aactacaacg ccacagcggc ggtgtatcag
     7081 aaagttgtga cactggaaga taaactggcg gagaaacctg tcacgggtaa gagccggaaa
     7141 aactgacgcg cttcgtttgt ccaacgcctc acggaccgcg aaaggatttc gcgaaccgtt
     7201 caccgtttcc atgagtgact cctccgaact gccaaaaaat atccctccct gacttgacag
     7261 agaaaataat ggcgagtact ttttgatctg tacataaaac cagctggttt tatgtacaga
     7321 tcaaaaagta ctcgccatta ttttctctgt caagtcaggg agggatattt tttggcagtt
     7381 cggaggagtc actcatggaa acggtgaacg gttcgcgaaa tcctttcgcg gtccgtgagg
     7441 cgttggacaa acgaagcgcg tcagtttttc cggctcttac ccgtgacagg tttctccgcc
     7501 agtttatctt ccagtgtcac aactttctga tacaccgccg ctgtggcgtt gtagttcctg
     7561 tcgagaattg ttttcatccc ccatccgagc acactgaaca taaccagaaa ggccactgac
     7621 accccaccca gccatttcca gctataccct ttaagggtgt tgtattcctg cacgatattg
     7681 ctgtgaattg tcgcaatatc cctgttcagg gcatccgcgt ttgccttcat gtttgcgact
     7741 gactcttcca gccgctgaaa atcctgactg atgcgctcac ctatcagccc tgcaacatcc
     7801 agcctctgca cttcgtttct gacttcatcc ctgacctgtt ttgccacacc gtcagaaata
     7861 tcccgatatc cgcgaagtgt ttcccttgcc acagccagag aatcagcaac agctttctgt
     7921 tgctccattg cggtatgttg cagatgctcc accgataaca ggagcgtgag taatttttct
     7981 tctgtgctgg ccatgttcag aatcccatag aaaatccacg tgacggtgat ttaacccgtt
     8041 caggttcagt tcggtcctgt ctgctcactc tgtctctcat gtcaaagcca tgctcccgcg
     8101 caacatcatg ttgaagcctt ctgaccagtt catgttgctt aaccggagtg tccagccatt
     8161 ccctgatgtc atgcccgacc gttcgcgcct gttcaagatg gcgggaatag tccggttcag
     8221 accgtttttg catgatgtca tgacgctggg catcatcaag ccgtccggca gcttcaaaac
     8281 ccagatgcac ttcgggacgt cgttcaatgc cctgttcttt cagcgtccgg cagtcaatga
     8341 agcccctttc aaagccatgc tttctcatat gttcgttgtg cttcacctcc cagcgttttc
     8401 gcaattcccg caactgctct ttttgctgag tgagagtcag gcttccggaa tcctttttcg
     8461 cacctccccg ctcgggatac ctggcgttat agcgccggaa atactgttcc ggtgtacggt
     8521 caatgccatc attcacacgc tggctcatca tgatatgcgc atgaggctgt tcgccgccgt
     8581 caatgctcgc tttcggattg tgaatggcaa aactccaggc gtgccgttcg ccggcttcct
     8641 gacgtacaaa atcctgcacc agttcaaggc gctgttccgg tgtcagttca cggggcagcg
     8701 cgatttccag ttcccgataa gtcgaaccgt tcgcacgttc aaactggtca gccgcctgcc
     8761 agaaatggga aggattatcc ctggcccatt ccggcatgtt tccgtgctcc gtgtattcca
     8821 gatctttccg tttcgcatac tcccctttgc gctcgatgta atcggcgtgg tttgcagcct
     8881 gtcccttgcc tccaaatttg acgctgaggt gataggttgc cacggtttca gccctccgca
     8941 ggagcgcact gtttctcgac gaaggagaga aacatataag tgcgcttcct tctaatttta
     9001 cgaattagca cggaagatac ccccctgcac ccaccgaccg caagcgatat actgcacgac
     9061 cacaacacac cgcaaaaaac accagggagc accacgaaca tgcgcatcag caacatcgaa
     9121 tggctgaaga aacggatcgg attcatcagg aagctcggag aacagaccgc acgacagaga
     9181 cagattattg acctgctcga caacgaagca ggactcacag aacaggaacg caaactcctc
     9241 cacgtactgg caacagcgga aaaaaaacga cttacaggca caggagagcg aacgaaaaca
     9301 ggcggtccaa aaacgaatcg agggaaaaaa acaacgacgg gaacgcaatc accggctctt
     9361 tctcgcggct gggctactga tcgaagcagg actggtagac acaaaaacag gcgagctatg
     9421 ctataaaaaa gacaggatat tacaggcact taaggaactt aagtacgatc tggagacatc
     9481 cccaaaccca gacgcgtaag tgcctccatc cgtgttccga ttccctggtg cttcctcgct
     9541 cattcgctcg ctacgctcgg tcatgagact gcggcgagca ctaccagcgc aatcatgcgg
     9601 aaaacagcac cattgcaggg ctgaacgggg aaaaacggca aaaaacctgc tgctcaaaaa
     9661 gacgccaaaa aggcgctgtt atctgatata ggcttatttg gtctactcta cgagacctcg
     9721 tttttttcag ttggtaaata ccaggcgcga ctgatcccta atgcctccca cggtttcagc
     9781 gtgcgtgcag atgtagccac tgtgctgcgc ttagacttag aaccgccctt gcgaccgcgt
     9841 gcagcctgaa tttctggtgt gtgcgtatca gccacatact gtgcgaaagt ttccggcgtg
     9901 aagttcctgt gcgtgtattt cgcaatgctc ctgccaatag cccgacattc aggcggtgaa
     9961 agcggaacgg gaagcgatgc gttgtacatt tcgacacgct gaatcacggc atcaagccac
    10021 tgtgagaatt caggccagcc ctgacgaatc gccctgtaag cccatttgcg cgtcatttcg
    10081 aacaggtggc agtttcgccc catcccgtaa tgtttatcga tgctacggcg cgctgaggcg
    10141 ctcaaatcga gataatcagc cagttcatcg agggtatagg cttcctctcg ccactccatc
    10201 acctgccatt ccaggtggaa cggattttta caaatcagtc cgctgtagtt cacatccgca
    10261 caaagtttct cacacagcga acgttcaatc gcagcggcgt atttcagcgc cttaaccgag
    10321 ctgtccggcg cggttctcac ggcgatattg agcgcgtaga gcaggtgagc atgaccgttc
    10381 acaggatttt ttacggtgat gttcggcgcg ggagcgttcc ggtcgctcca gtcaatcgct
    10441 gccccctcgc ggtcaacgtc aaaaaaccag ccagaactgt gcatgaggct ggttctgctg
    10501 tatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaagaccaa aagcgagatc
    10561 atccgtgtga tagggacggt ggggtaaatt ttcgtcgaag tattgaagcg cggcgctcat
    10621 gtgttacgat tctctccggg ttatgcgacc tacacgccct ctgtatttca tgttgaatct
    10681 tgccggatct ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa
    10741 cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt taccataact ccgttaaacg
    10801 caccgctaaa acgcgctcaa tcgctttctg acgcaaaaac acgcaaaaag acaaggaaac
    10861 accaggctga accagaaaaa tgcgtcagaa tcgtttttta gggcctgcat aactatgcac
    10921 gaaagcgatg tgcgatcggt ttcgtgcgtt ctgaacaccg aacggctcaa aacgaaaagt
    10981 tttctccctc ctgatggaag gtttctgcac ggagatttta gatcccatct cccacatgga
    11041 atttttgtgg cacttcaaca aaaaaagcgc gtcagaatca gcctgacacg ctttgttctt
    11101 gtcaaatgag gattgcaggt tactgcgttt ccactccggt cagttcagca gtt
//
