LOCUS       NZ_MT090963             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2079 plasmid pKP18-2079_5kb,
            complete sequence.
ACCESSION   NZ_MT090963
VERSION     NZ_MT090963.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qin,S. and Li,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2020) Zhengzhou University, Institute of Drug
            Discovery & Development, 100 Science Avenue, Zhengzhou City,
            Zhengzhou, Henan 450001, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 10.0
            Sequencing Technology             :: Illumina; PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 00:27:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT090963.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2079"
                     /isolation_source="blood from male"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2079_5kb"
                     /geo_loc_name="China"
                     /collection_date="22-Nov-2018"
     gene            513..671
                     /locus_tag="H5H27_RS00065"
     CDS             513..671
                     /locus_tag="H5H27_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1029..1355
                     /locus_tag="H5H27_RS00005"
     CDS             1029..1355
                     /locus_tag="H5H27_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1381..1926
                     /locus_tag="H5H27_RS00010"
     CDS             1381..1926
                     /locus_tag="H5H27_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            1992..2342
                     /locus_tag="H5H27_RS00015"
     CDS             1992..2342
                     /locus_tag="H5H27_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2412..2678)
                     /locus_tag="H5H27_RS00020"
     CDS             complement(2412..2678)
                     /locus_tag="H5H27_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2662..2886)
                     /locus_tag="H5H27_RS00025"
     CDS             complement(2662..2886)
                     /locus_tag="H5H27_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3049..3177
                     /locus_tag="H5H27_RS00055"
     CDS             3049..3177
                     /locus_tag="H5H27_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(3553..3765)
                     /locus_tag="H5H27_RS00030"
     CDS             complement(3553..3765)
                     /locus_tag="H5H27_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3772..4281)
                     /locus_tag="H5H27_RS00035"
     CDS             complement(3772..4281)
                     /locus_tag="H5H27_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4316..5020)
                     /locus_tag="H5H27_RS00070"
                     /pseudo=""
     CDS             complement(<4316..5020)
                     /locus_tag="H5H27_RS00070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4959..5303)
                     /locus_tag="H5H27_RS00045"
     CDS             complement(4959..5303)
                     /locus_tag="H5H27_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5293..5469)
                     /locus_tag="H5H27_RS00050"
     CDS             complement(5293..5469)
                     /locus_tag="H5H27_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          225..622
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3438..4969)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tacgctgtgt atactggctt actatgttgg catgagagca cgattcatga aagtgcctca
       61 tgtggcaggt gtgatatgtg gctcaggagc gtccgcaata ccatgcacgg acagatgaaa
      121 tgacgcctcc tcgctcactc ggtagctgcg ctcctgccgt tcggctgcgg cgggcgtgac
      181 cgactcacgg ataacgggat aaagcaaagg cgctcaaatc agaaagggcc ggagaaaagg
      241 atgcggcgtt gccgtttttc cataggctcc gcctcccctg acgagcatca cagaaatgga
      301 cgctcaagtc agaggaggac gaaacccgac aggacttaaa gatgccggtg atttctctcc
      361 ggcggctccc tcgtgcgctc tcctgttccg accctgccgt ttaccggata cctgttccgc
      421 ctttttccct ttcgggaagc gtggcgcttt ctcatagctc acgctgctgg tatctcggct
      481 cggtttaggt cgttcgctcc aagctgggct gtatgcacga accccccgtt cagcccgact
      541 gctgcgccct ttccggtaac tatcatcttg agtccaaccc ggacggacac gcaaaaacgc
      601 cactggcagc agccattggt aactggatat atacgagaga gatattgaga ggtcttgaag
      661 tgggtgccta actgcggcta cactgaaaga acagttttgg tatctgcggt ctcgtatacc
      721 cagttacccg gttaagcagt tccccaactg acttaacctt cgatcaaacc gcctccccag
      781 gcggtttttt cgtttacagt gcaagagatt acgcgcagaa aaaaaggatc tcaagaagat
      841 cctttaaatg atcgtcagtt ctgttatccc cggagctcca tccatcccac ggaaaaacac
      901 ccgccactga tgccctcagc gcactgcaca tcatgtacaa accactccct ccctcctttt
      961 ttattgcgtt tcttaatgcg caatggtagc tttgtgcgca acaaaaaacg caatagcaag
     1021 ggggggtaat gagagaaaga acaaatatca tgctagatat aggattaaag caatccttac
     1081 aacggctttc gaataaaacc aaaatgagtc taagtgatct tattaaccag agattatcag
     1141 gttcactttc agttgaagaa cgcattggcg aaaagcctga atggatgaga aaccttgatc
     1201 cctatctcgc caatcacttt tcaaatattg agttaccgct cccttcacca ttaaaagaat
     1261 accaagaaaa aatggatgag atagattcaa ttattaatga catgatggtt ctaaaggaat
     1321 atttcagagc taagttttac gacgaaataa aataggcacg ctatacggag gcggagaatt
     1381 atgtttttta aacggtattt aacggagaag gaaatataca aggcagcact cccaattgtt
     1441 aggcttcaag ggagagttaa cacttcatta cgttttttat ccaaatactc agatgtaaac
     1501 aaaaatggat taattgcatt gcatcttaat ttattacatt ttatctcata caacatacac
     1561 ggtgtagagt ctctgaaaat cactcaagca ggcataaaca aagctgtaca acaaagccct
     1621 cacgcagatg aaataaaaaa tattgcgcag gcaataggac gcaaaggacc tgagtacatc
     1681 gaaggattaa ttagaaaccc atgggggaag aaaaatgata atccactaat atttataatg
     1741 agcattgaag aaagtataaa agaaaccctt aactcaggtt atatgttata tagagtagct
     1801 cccataactg agggtgaagc agctgctttc atggatgttc ttaaacaatt attattattt
     1861 gaggataatt ttcgcctttt tggtagagac ttggtttata aaatagaaaa attaaaaatc
     1921 agataacaaa gtatggtgaa cgaaaaatga aagagaggat agtgaaatgt gtaataccaa
     1981 gattctcaac tatgtattgg ttaattgcgt tcctagcatt tatattcccc ggtcgatctt
     2041 ttgctgacgg ttacaccgct tactaccgat gtaatggtac tcaggaagca tctcttactg
     2101 ttaaagataa caaactaagt atggttgtag gtctttctaa gtttaatagg caactggata
     2161 cttatagatt caaagaaaag ggaatcgaag tagtaatgca gagagctgct tctgatgata
     2221 actctgaggc cataatgtct tttattatgc ctgacgttaa caatatgacc ttattagtta
     2281 aaaataacgg ctacattatc gttaagacaa cctgctggtt aactaaaacc gacaaacagt
     2341 agaacacgta gttagtcgag tatttaccat aatacctgtt gtcggcatca aaaaagcccg
     2401 catagcgggc ttcagtcgta aacttcgcgg cgatgtccaa tcgtggcggc aaggataaca
     2461 agctcgtcat ctcggatctc acacagaacg cgataatctc ccacccgata gcgccagaac
     2521 tcgcccagct cacctttgag cggcttccct gactggcgag gatcggcagc aactgcaatg
     2581 cgtaaactca taaaatccac aatccgtcgt gcgttctgtt tgtccatctt gcgtaacgat
     2641 ttgagcgccc gatcggaata gttaatcgtc cagaccaagt gacttcctca cgtcttcaga
     2701 cgaatgcact gcttcatcac cacggcgaac gcgtgcagca gtttctgctg aaagataata
     2761 atcctccagg tcttccagat gcgccagtat tgcttcccgc gcataaaacg tctttgttct
     2821 gccggtttgc ttcgccagcg agtccagacg ggactcaatc tcatcagata accggatagc
     2881 aagcatataa acctcccgaa caccatttgc tatacatgta tatcaacagt accacaatca
     2941 acctaaaaga acaaaaaata atcttgttag cccctcttga taatccgtaa cgcggtcaat
     3001 atatctagct tgagggcttt cctcttaact attgaaagga taacgattat gggtcttaag
     3061 ccgggtccga aacgaattgc cgattcaaca ggggaacctg ataaacgcca gcgtgacaat
     3121 aaaaaaacgc cgggaaatac tgacaaattg aaacccagca aatcttcaaa aaaataagtc
     3181 agtagttaaa agccctccgg ggcttttttg tatctgccgt tcacgctgat taaacataac
     3241 acccgttgcc cgcaccgccc cggacgggtt cgccgcgaac ggggtggcag tcaggtaaaa
     3301 ccggcaacaa accacccgat aaaccgcccc gttcgcgaca gataaatttt acctggctgc
     3361 atagccatgc agccaggtga atgacatccg gtttcagcgt aaatgcccga tgagaaccct
     3421 gttttcctgc atttcagccc agcgacggac cgtcatagct tcgctgccgg gaacgcgctt
     3481 ccgtcagttc cttttcgtgc tgccgttgct cgtgcgcccg ttctgcggcc acggccatct
     3541 catgacgctg cactaccggc tcaaacgctg cagttgcccg ctcaagcgtt ccacctgctg
     3601 actcaagcgc gtcacgcgct cgctcagcgc cgcgttctcc cgttgcataa gccctgacat
     3661 tttccgccat tccccgaagg cgctctccca ctcgtccagc cttttcgagt agtcctgctg
     3721 tagctgctct aatgcgctca gcaactgttt ttccagctcg gtcatcggca cttactcctt
     3781 caccagtctc agcgcgttct gcttcgaccg cccgttcccg accgtccagc tctgcgtcct
     3841 gtctacggcc gtccccttcg gcagcaccag aaaacgtccg ttgctgtctt ccaggtacgt
     3901 cacgccccag gtcttcgcgt tcagcttcga cagcgctgcg ttctgccggt cgatttcggc
     3961 cagatttgac gctatcaagc tcccctgata ccacaacacc ccgctcaggc tggcgaacag
     4021 cagtcctgac accatcgtga tggtcagcca tgtccgcccg accatacgca tcacgctcag
     4081 gcggttcgac tgcatggccg cgctcatacc ctgctcgtgg gcgcggatgg cggcgctgat
     4141 tctcttcccg ctttcgctca gttccgctct gacagacttc tcgtgctcgc tgaatgcggc
     4201 tttcagcatt tcgccggtac tctgctgctg cgctttcgat ttctgctcta agtccttcgc
     4261 cagcgttaaa agactgttca tagatggctc ccttaagtcg gatgtttcgc cctccgtccg
     4321 ggtcggcaat gctgatgctg ctctttgtgg tgcgcacgac ctcaaaacct gcgctttcca
     4381 gcgcctcagt gacgtcctga cgcgttttaa gctcccctga cgaggcgagg gccagtaagc
     4441 cccgcgtaat agcctctgcg gcttcctgct tcgttttggg taacgaagat ggcgtcacca
     4501 gcgcgcgccg gttctccggt gcgttcgggt cgtgcagccc cagtcgcccg ttcaccacgg
     4561 tctgccacgc atcgatgcgc ggacggtctg cccggtcgta gtacggctgg agacgcctgc
     4621 cggtcagcag ttccgtgttc ggtaccagaa aattcagttc cagccgcccc ttgtcccggt
     4681 gttcaaccca cagcacgctg tactggtctt tatcgagtcc gggcatcaga acccgctcga
     4741 agctcgccat cagcttttca cgctggccgg gcggtaaatc ctgttcagca aaagacagga
     4801 cgccggaggt gtactttttg gcgtagggcg aggcgtcgat aagctcccgg acctcctccg
     4861 gctttccctg caggacgatg gccccgtcgc gctggcggtc tttccccagc agataatcca
     4921 ccggcccggc accgccgcct cgccctcgcg gatgaaactt aacgatcatc gtctgccccc
     4981 ttttccagca cggcatgccg cagccgctcg agttcggcat caatggccat cagcgcggcc
     5041 accacctgaa cccggtcagc gcctgaccac tggccgccgt taattttccg ggcgatctgg
     5101 ttgaggttgt tccccatccc ggcaagctgg cgcagcaaaa ccggatcgat ggacggcagc
     5161 cgcaacgacc gcgccgggcg cgtatcgaga caggtttccc gcatccacac cgccagttgc
     5221 cttccattgc agcgctccag cagctgctgg tactccccat cggtcacccg gatacctaaa
     5281 aaacgggtac gcttaaccgc catcctctcc tccgatatcc gcaaaatgtg accgggctga
     5341 acgctcaagc tctgccatct gctcccggcg gtaatcttcc tcctgtgcct gacgcatcag
     5401 atttgccatc actgccggat gccaggatct tttttttacg acgagcggct tcttcttcgg
     5461 cttctgcatt atccctcctt cgcaaacgcc ggacggcacg gaaaagtttt ccgggtcgct
     5521 ccggcgttgg acaagcgcca gcgcgtcagt ggtgttagcg ggtttcgggg cgcagccctg
     5581 aaccagtcac agcgtt
//
