LOCUS       NZ_MT035878             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-3-8 plasmid pKP18-3-8_5_KB,
            complete sequence.
ACCESSION   NZ_MT035878
VERSION     NZ_MT035878.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qin,S. and Li,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2020) Zhengzhou University, Institute of Drug
            Discovery & Development, 100 Science Avenue, Zhengzhou City,
            Zhengzhou, Henan 450001, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 10.0
            Sequencing Technology             :: Illumina; PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 00:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 13
            CDSs (total)                      :: 13
            Genes (coding)                    :: 12
            CDSs (with protein)               :: 12
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT035878.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-3-8"
                     /isolation_source="Urine"
                     /host="Homo sapiens male"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-3-8_5_KB"
                     /geo_loc_name="China"
                     /collection_date="14-Jul-2018"
                     /collected_by="Starin"
     gene            join(5473..5596,1..122)
                     /locus_tag="H5H01_RS00055"
     CDS             join(5473..5596,1..122)
                     /locus_tag="H5H01_RS00055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     gene            complement(304..648)
                     /locus_tag="H5H01_RS00010"
     CDS             complement(304..648)
                     /locus_tag="H5H01_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(638..814)
                     /locus_tag="H5H01_RS00015"
     CDS             complement(638..814)
                     /locus_tag="H5H01_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1454..1612
                     /locus_tag="H5H01_RS00070"
     CDS             1454..1612
                     /locus_tag="H5H01_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1970..2296
                     /locus_tag="H5H01_RS00020"
     CDS             1970..2296
                     /locus_tag="H5H01_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            2322..2867
                     /locus_tag="H5H01_RS00025"
     CDS             2322..2867
                     /locus_tag="H5H01_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2933..3283
                     /locus_tag="H5H01_RS00030"
     CDS             2933..3283
                     /locus_tag="H5H01_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3353..3619)
                     /locus_tag="H5H01_RS00035"
     CDS             complement(3353..3619)
                     /locus_tag="H5H01_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3603..3827)
                     /locus_tag="H5H01_RS00040"
     CDS             complement(3603..3827)
                     /locus_tag="H5H01_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3990..4118
                     /locus_tag="H5H01_RS00065"
     CDS             3990..4118
                     /locus_tag="H5H01_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(4494..4706)
                     /locus_tag="H5H01_RS00045"
     CDS             complement(4494..4706)
                     /locus_tag="H5H01_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4713..5222)
                     /locus_tag="H5H01_RS00050"
     CDS             complement(4713..5222)
                     /locus_tag="H5H01_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5257..5589)
                     /locus_tag="H5H01_RS00060"
                     /pseudo=""
     CDS             complement(<5257..5589)
                     /locus_tag="H5H01_RS00060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(901..977)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1166..1563
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(4..314)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="86.0"
                     /evalue="2.640000000000001e-59"
     Typing          complement(4379..5595)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.754"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agttccagcc gccccttgtc ccggtgttca acccacagca cgctgtactg gtctttatcg
       61 agtccgggca tcagaacccg ctcgaagctc gccatcagct tttcacgctg gccgggcggt
      121 aaatcctgtt cagcaaaaga caggacgccg gaggtgtact ttttggcgta gggcgaggcg
      181 tcgataagct cccggacctc ctccggcttt ccctgcagga cgatggcccc gtcgcgctgg
      241 cggtctttcc ccagcagata atccaccggc ccggcaccgc cgcctcgccc tcgcggatga
      301 aacttaacga tcatcgtctg cccccttttc cagcacggca tgccgcagcc gctcgagttc
      361 ggcatcaatg gccatcagcg cggccaccac ctgaacccgg tcagcgcctg accactggcc
      421 gccgttaatt ttccgggcga tctggttgag gttgttcccc atcccggcaa gctggcgcag
      481 caaaaccgga tcgatggacg gcagccgcaa cgaccgcgcc gggcgcgtat cgagacaggt
      541 ttcccgcatc cacaccgcca gttgccttcc attgcagcgc tccagcagct gctggtactc
      601 cccatcggtc acccggatac ctaaaaaacg ggtacgctta accgccatcc tctcctccga
      661 tatccgcaaa atgtgaccgg gctgaacgct caagctctgc catctgctcc cggcggtaat
      721 cttcctcctg tgcctgacgc atcagatttg ccatcactgc cggatgccag gatctttttt
      781 ttacgacgag cggcttcttc ttcggcttct gcattatccc tccttcgcaa acgccggacg
      841 gcacggaaaa gttttccggg tcgctccggc gttggacaag cgccagcgcg tcagtggtgt
      901 tagcgggttt cggggcgcag ccctgaacca gtcacagcgt ttacgctgtg tatactggct
      961 tactatgttg gcatgagagc acgattcatg aaagtgcctc atgtggcagg tgtgatatgt
     1021 ggctcaggag cgtccgcaat accatgcacg gacagatgaa atgacgcctc ctcgctcact
     1081 cggtagctgc gctcctgccg ttcggctgcg gcgggcgtga ccgactcacg gataacggga
     1141 taaagcaaag gcgctcaaat cagaaagggc cggagaaaag gatgcggcgt tgccgttttt
     1201 ccataggctc cgcctcccct gacgagcatc acagaaatgg acgctcaagt cagaggagga
     1261 cgaaacccga caggacttaa agatgccggt gatttctctc cggcggctcc ctcgtgcgct
     1321 ctcctgttcc gaccctgccg tttaccggat acctgttccg cctttttccc tttcgggaag
     1381 cgtggcgctt tctcatagct cacgctgctg gtatctcggc tcggtttagg tcgttcgctc
     1441 caagctgggc tgtatgcacg aaccccccgt tcagcccgac tgctgcgccc tttccggtaa
     1501 ctatcatctt gagtccaacc cggacggaca cgcaaaaacg ccactggcag cagccattgg
     1561 taactggata tatacgagag agatattgag aggtcttgaa gtgggtgcct aactgcggct
     1621 acactgaaag aacagttttg gtatctgcgg tctcgtatac ccagttaccc ggttaagcag
     1681 ttccccaact gacttaacct tcgatcaaac cgcctcccca ggcggttttt tcgtttacag
     1741 tgcaagagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttaaat gatcgtcagt
     1801 tctgttatcc ccggagctcc atccatccca cggaaaaaca cccgccactg atgccctcag
     1861 cgcactgcac atcatgtaca aaccactccc tccctccttt tttattgcgt ttcttaatgc
     1921 gcaatggtag ctttgtgcgc aacaaaaaac gcaatagcaa gggggggtaa tgagagaaag
     1981 aacaaatatc atgctagata taggattaaa gcaatcctta caacggcttt cgaataaaac
     2041 caaaatgagt ctaagtgatc ttattaacca gagattatca ggttcacttt cagttgaaga
     2101 acgcattggc gaaaagcctg aatggatgag aaaccttgat ccctatctcg ccaatcactt
     2161 ttcaaatatt gagttaccgc tcccttcacc attaaaagaa taccaagaaa aaatggatga
     2221 gatagattca attattaatg acatgatggt tctaaaggaa tatttcagag ctaagtttta
     2281 cgacgaaata aaataggcac gctatacgga ggcggagaat tatgtttttt aaacggtatt
     2341 taacggagaa ggaaatatac aaggcagcac tcccaattgt taggcttcaa gggagagtta
     2401 acacttcatt acgtttttta tccaaatact cagatgtaaa caaaaatgga ttaattgcat
     2461 tgcatcttaa tttattacat tttatctcat acaacataca cggtgtagag tctctgaaaa
     2521 tcactcaagc aggcataaac aaagctgtac aacaaagccc tcacgcagat gaaataaaaa
     2581 atattgcgca ggcaatagga cgcaaaggac ctgagtacat cgaaggatta attagaaacc
     2641 catgggggaa gaaaaatgat aatccactaa tatttataat gagcattgaa gaaagtataa
     2701 aagaaaccct taactcaggt tatatgttat atagagtagc tcccataact gagggtgaag
     2761 cagctgcttt catggatgtt cttaaacaat tattattatt tgaggataat tttcgccttt
     2821 ttggtagaga cttggtttat aaaatagaaa aattaaaaat cagataacaa agtatggtga
     2881 acgaaaaatg aaagagagga tagtgaaatg tgtaatacca agattctcaa ctatgtattg
     2941 gttaattgcg ttcctagcat ttatattccc cggtcgatct tttgctgacg gttacaccgc
     3001 ttactaccga tgtaatggta ctcaggaagc atctcttact gttaaagata acaaactaag
     3061 tatggttgta ggtctttcta agtttaatag gcaactggat acttatagat tcaaagaaaa
     3121 gggaatcgaa gtagtaatgc agagagctgc ttctgatgat aactctgagg ccataatgtc
     3181 ttttattatg cctgacgtta acaatatgac cttattagtt aaaaataacg gctacattat
     3241 cgttaagaca acctgctggt taactaaaac cgacaaacag tagaacacgt agttagtcga
     3301 gtatttacca taatacctgt tgtcggcatc aaaaaagccc gcatagcggg cttcagtcgt
     3361 aaacttcgcg gcgatgtcca atcgtggcgg caaggataac aagctcgtca tctcggatct
     3421 cacacagaac gcgataatct cccacccgat agcgccagaa ctcgcccagc tcacctttga
     3481 gcggcttccc tgactggcga ggatcggcag caactgcaat gcgtaaactc ataaaatcca
     3541 caatccgtcg tgcgttctgt ttgtccatct tgcgtaacga tttgagcgcc cgatcggaat
     3601 agttaatcgt ccagaccaag tgacttcctc acgtcttcag acgaatgcac tgcttcatca
     3661 ccacggcgaa cgcgtgcagc agtttctgct gaaagataat aatcctccag gtcttccaga
     3721 tgcgccagta ttgcttcccg cgcataaaac gtctttgttc tgccggtttg cttcgccagc
     3781 gagtccagac gggactcaat ctcatcagat aaccggatag caagcatata aacctcccga
     3841 acaccatttg ctatacatgt atatcaacag taccacaatc aacctaaaag aacaaaaaat
     3901 aatcttgtta gcccctcttg ataatccgta acgcggtcaa tatatctagc ttgagggctt
     3961 tcctcttaac tattgaaagg ataacgatta tgggtcttaa gccgggtccg aaacgaattg
     4021 ccgattcaac aggggaacct gataaacgcc agcgtgacaa taaaaaaacg ccgggaaata
     4081 ctgacaaatt gaaacccagc aaatcttcaa aaaaataagt cagtagttaa aagccctccg
     4141 gggctttttt gtatctgccg ttcacgctga ttaaacataa cacccgttgc ccgcaccgcc
     4201 ccggacgggt tcgccgcgaa cggggtggca gtcaggtaaa accggcaaca aaccacccga
     4261 taaaccgccc cgttcgcgac agataaattt tacctggctg catagccatg cagccaggtg
     4321 aatgacatcc ggtttcagcg taaatgcccg atgagaaccc tgttttcctg catttcagcc
     4381 cagcgacgga ccgtcatagc ttcgctgccg ggaacgcgct tccgtcagtt ccttttcgtg
     4441 ctgccgttgc tcgtgcgccc gttctgcggc cacggccatc tcatgacgct gcactaccgg
     4501 ctcaaacgct gcagttgccc gctcaagcgt tccacctgct gactcaagcg cgtcacgcgc
     4561 tcgctcagcg ccgcgttctc ccgttgcata agccctgaca ttttccgcca ttccccgaag
     4621 gcgctctccc actcgtccag ccttttcgag tagtcctgct gtagctgctc taatgcgctc
     4681 agcaactgtt tttccagctc ggtcatcggc acttactcct tcaccagtct cagcgcgttc
     4741 tgcttcgacc gcccgttccc gaccgtccag ctctgcgtcc tgtctacggc cgtccccttc
     4801 ggcagcacca gaaaacgtcc gttgctgtct tccaggtacg tcacgcccca ggtcttcgcg
     4861 ttcagcttcg acagcgctgc gttctgccgg tcgatttcgg ccagatttga cgctatcaag
     4921 ctcccctgat accacaacac cccgctcagg ctggcgaaca gcagtcctga caccatcgtg
     4981 atggtcagcc atgtccgccc gaccatacgc atcacgctca ggcggttcga ctgcatggcc
     5041 gcgctcatac cctgctcgtg ggcgcggatg gcggcgctga ttctcttccc gctttcgctc
     5101 agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg ctttcagcat ttcgccggta
     5161 ctctgctgct gcgctttcga tttctgctct aagtccttcg ccagcgttaa aagactgttc
     5221 atagatggct cccttaagtc ggatgtttcg ccctccgtcc gggtcggcaa tgctgatgct
     5281 gctctttgtg gtgcgcacga cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg
     5341 acgcgtttta agctcccctg acgaggcgag ggccagtaag ccccgcgtaa tagcctctgc
     5401 ggcttcctgc ttcgttttgg gtaacgaaga tggcgtcacc agcgcgcgcc ggttctccgg
     5461 tgcgttcggg tcgtgcagcc ccagtcgccc gttcaccacg gtctgccacg catcgatgcg
     5521 cggacggtct gcccggtcgt agtacggctg gagacgcctg ccggtcagca gttccgtgtt
     5581 cggtaccaga aaattc
//
