LOCUS       NZ_MT035877            10060 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-3-8 plasmid pKP18-3-8_COL,
            complete sequence.
ACCESSION   NZ_MT035877
VERSION     NZ_MT035877.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Qin,S. and Li,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2020) Zhengzhou University, Institute of Drug
            Discovery & Development, 100 Science Avenue, Zhengzhou City,
            Zhengzhou, Henan 450001, China
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: CLC Genomics Workbench v. 10.0
            Sequencing Technology        :: Illumina; PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/10/2024 00:25:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MT035877.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-3-8"
                     /isolation_source="Urine"
                     /host="Homo sapiens male"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-3-8_COL"
                     /geo_loc_name="China"
                     /collection_date="14-Jul-2018"
                     /collected_by="Starin"
     gene            255..1940
                     /locus_tag="H5H03_RS00005"
     CDS             255..1940
                     /locus_tag="H5H03_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1950..2207
                     /locus_tag="H5H03_RS00010"
     CDS             1950..2207
                     /locus_tag="H5H03_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2292..2441
                     /locus_tag="H5H03_RS00015"
     CDS             2292..2441
                     /locus_tag="H5H03_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3925..5889
                     /locus_tag="H5H03_RS00020"
     CDS             3925..5889
                     /locus_tag="H5H03_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5889..6620
                     /gene="mobC"
                     /locus_tag="H5H03_RS00025"
     CDS             5889..6620
                     /gene="mobC"
                     /locus_tag="H5H03_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6627..7157
                     /locus_tag="H5H03_RS00030"
     CDS             6627..7157
                     /locus_tag="H5H03_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137970705.1"
                     /translation="MALYMVFIEDVEDKDGEHCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7184..7363)
                     /locus_tag="H5H03_RS00035"
     CDS             complement(7184..7363)
                     /locus_tag="H5H03_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7389..7817)
                     /locus_tag="H5H03_RS00040"
     CDS             complement(7389..7817)
                     /locus_tag="H5H03_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7981..8295)
                     /locus_tag="H5H03_RS00045"
     CDS             complement(7981..8295)
                     /locus_tag="H5H03_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8283..8561)
                     /locus_tag="H5H03_RS00050"
     CDS             complement(8283..8561)
                     /locus_tag="H5H03_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8736..9101)
                     /locus_tag="H5H03_RS00070"
     CDS             complement(8736..9101)
                     /locus_tag="H5H03_RS00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(9098..9469)
                     /gene="tolA"
                     /locus_tag="H5H03_RS00060"
     CDS             complement(9098..9469)
                     /gene="tolA"
                     /locus_tag="H5H03_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9743..9988)
                     /locus_tag="H5H03_RS00065"
     CDS             complement(9743..9988)
                     /locus_tag="H5H03_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..6940
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2545..3123)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5890..6617
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 agatgtgtct ataccatctg aaatgtaagt gtgatgaatg tcttaaataa taaaatttgt
       61 tgccatgaga aattttcggg aaaagacgcg atctgcttac aggtggctcc gggcgctttt
      121 ttgtgcctga agatgagaac taatctcata tattgacacc tgaaaacggg aggagtaagg
      181 taataattat actgtgtata tgtacagtat gttggcttta atttattgtt ttaaatgtca
      241 aagaggaaaa cggtatgagt ggtggagacg gtcgaggtcc gggtaattca ggtctgggac
      301 ataatggtgg tcaggccagt gggaatgtga acggtacgtc tggtaaaggt ggcccttcat
      361 caggtggtgg tacggatcca aacagcgggc cgggctgggg tacgacgcat acgccgaatg
      421 gagatattca taactataat ccgggggagt ttggtaacgg cgggagtaaa cccggtggta
      481 atggtggtaa cagcggcaat catagcggta gctctggtgg cggacagtct tcggccactg
      541 cgatggcctt cggtctgcct gccctggcta ctccgggggc tgaaggactg gctttatcag
      601 tttccggcga tgcgttgtcg gccgccgttg ctgatgtgct ggctgccctg aaggggccgt
      661 ttaagtttgg tctgtggggg attgcgatct acggtgtgct gccttctgag attgcaaaag
      721 atgatccgaa aatgatgtca aaaattatga cgtcattacc ggccgatgcg gtgacggaga
      781 ctccggcaag tactttacca ctggaccagg cgacggttcg tgtcagacaa cgggttgtgg
      841 atgtggtgaa ggatgagcgg cagcatattg cggttgtcgc aggtcggcca atgagtgtcc
      901 ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt cagtgtgtcg attccgggtc
      961 tcccgtctct gcaggtgagc gtaccgaaag gtgttccgac agcgaaagcc ccgccaaaag
     1021 gcattgttgc tgaaaaaggt gattcacgtc cggctggttt tacagccggt ggtaactccc
     1081 gtgaggccgt tattcgtttc ccgaaagaga ccggacagaa gccggtttat gtgtcggtga
     1141 cagatgttct taccccggca caggtaaaac agcgtcagga ggaagaaaag cgtcgccagc
     1201 aggcatggga cgccgctcat ccggaagagg ggctgaaaag agactatgat aaagcgaaag
     1261 ctgagctgga tgccgaagat aaaaatattg cgaccttaaa cagccgcatt gcatcgacag
     1321 agaaggcgct ccccggtgca agggctgctg ttcaggaagc cgataaaaag gtaaaagagg
     1381 cagaggcaaa taaagatgat tttgtgactt ataacccgcc tcatgaatat ggctccgggt
     1441 ggcaggatca ggttcgctat cttgataagg atattcagaa tcagaatgag aaattaaaag
     1501 cggctcaggc atctttaaac gcaatgaatg aatccttatc cagagataag gctgcactta
     1561 ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca gaaaataagt
     1621 taaatgagga aaagaacaag cctcgcaagg ggactaaaga ctacggccat gattatcatc
     1681 cagtacctaa aacagaagac ataaagggac ttggagagtt aaagcgaggt gatcctaaaa
     1741 cacctaaaca aggtggtggt ggtaagcggg cccgatggta tggcgataaa aaacgtaaaa
     1801 tttatgaatg ggattcccag cacggtgagc ttgaagggta tcgtgccagt gatggcgaac
     1861 acctaggcgc atttgatccc aaaacgggta agcaggttaa agggccagat ccaaaacgga
     1921 acattaaaaa atatctttga gaggtaaata tggggcttaa attacgtttg acatggtttg
     1981 ataagaaaac tgaagagttt aaaggggaag agtattctaa agactttggt gacgatggtt
     2041 ctgtcattga aagcttggga atgcctttaa aggataatat taataatggt gagtttgatt
     2101 tgaagaaaga atgggttcct ttactacagc cattttttaa aaatcaaata aaacctgatg
     2161 tggataattg ttatatagct tttgattatc gggacggtaa ctggtaacaa aaaattatca
     2221 aggatgattt gaaatactga cttccggtgt tcagggatga atgccggagt ctttaaatac
     2281 agggaaagta tatgaaaaag gtaaagacta tttttctttt tatattgatt gttgctggct
     2341 ttcttctggc cgcatgtcag gcaaattata tccgggatgt tcagggcgga acggtggcac
     2401 catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc
     2461 ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg
     2521 aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt aactggcttt
     2581 cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc gcacacttca
     2641 agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac caatggctgc
     2701 tgccagtggc gttttgcgtg ctttcccggg ttggactcaa gtgaacagtt accggataag
     2761 gcgcagcagt cgggctgaac ggggagttct tgcttacagc ccagcttgga gcgaacgacc
     2821 tacaccgagc cgagatacca gtgtgtgagc tatgagaaag cgccacactt cccgcaaggg
     2881 agaacggcgg acaggtatcc ggtaaacggc agggtcggaa caggagagcg caagagggag
     2941 cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga
     3001 ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt ttatgtgaac
     3061 tctggcagga gggcggagcc tatggaaaaa cgccaccggc gcggccctgc cgttttgcct
     3121 cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg tgtccgcagc
     3181 gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg cgcaatttcc
     3241 ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc ctgattaagt
     3301 ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt tttcgctgaa
     3361 cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg caagtcatcc
     3421 ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct ggacgccacc
     3481 gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc cacgccagaa
     3541 attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac ggtgctggac
     3601 gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg ctcgctatgg
     3661 cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg ccttttcctc
     3721 tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc atggggcgga
     3781 aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc acggacgtga ccgccgccgc
     3841 catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg ccggtaaacc
     3901 cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg gcagtggcat
     3961 atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtaccg ggagtattac
     4021 agtctggccg gtttttttct ggttatcggc ctgtttttcg gtctggctga actctgggcc
     4081 ggccgaatgc gttttgtggg gctgagccat aaggcgcggc gggagtatgc cgatcgctat
     4141 aagcgcgata ataaccggac tgtgccattt tcggtgctgg cctcgctgga ttcacccggg
     4201 cggatggtca gggcacggcg acatgtctgg aactacgcgt actggctgac cggcgctgcc
     4261 ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg gggagaaacc
     4321 gtcctgcgcc cggacctcta tgcgaacgca gattattacc tgctgcagcc ggctaacctg
     4381 ttcgggatag cctggtgtgc cggagggctt gtcgccggcg tccttctggc gcggctgctg
     4441 gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa cgcgaaactg
     4501 aatcaggaga gccggcgcag cagccagcgg accggagaaa tgacagacgt ccgccacctg
     4561 catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga gcaggcccgg
     4621 aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct ggtccctcgt
     4681 gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg caagagcgcg
     4741 atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt ggtgtacttc
     4801 gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc ggacttcacc
     4861 ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga tctcgaccag
     4921 tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg cgacgtggcg
     4981 gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gttcccgcag
     5041 ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc cctgaaaaag
     5101 gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt cctgcagacg
     5161 gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta cgtcatcggc
     5221 tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg ctgcgcgcaa
     5281 atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat gctcgatgag
     5341 attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg cagccgggac
     5401 tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg gcaggatctg
     5461 cccgctgatt ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttctatcgc
     5521 gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggagat ccgggtggac
     5581 gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcacatcag cggcgacagc
     5641 ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca cctgcctgac
     5701 gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag cccgctgcgg
     5761 gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac ggacccgctg
     5821 gcggagtacc tgcctccggc cgacggcggc ggaccaaaag cggatgatga ttttgcgggg
     5881 ctttactgat gacactggag cagtacaacg tcagccacgc gaagcggcag gcgcggaacg
     5941 cggagaaaac acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc acggcggagc
     6001 tggttgcgcg acggctgggg attgcggcgg tacagccggt ttaccgcttc ctggacagtc
     6061 tggtggcgaa agggctgctg gtccgggcga aatacccggt tgacggccgt caggtcagcg
     6121 tgtgggggct gacgccgcac ggggtggcgt tctcgttcga cgaggacgag ccgctgacgg
     6181 acgttattcc gtttcagccg tcaagggtgt cggcggcgca gctgccgcac cggctggccg
     6241 tgcagtccct gcgcctctcc atggagggcc ggggggccac cggctggcgg tacctgcacc
     6301 gtatggccct gaaagggatg aaagtgccgg acgcgctggc ggagctggac ggcagaacgg
     6361 tggcatttga ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa gtggtgtccg
     6421 ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat ctggtacatc tgccctgacc
     6481 ggccgacgct tgtccgggta cagcgggcga ttctggcggt ggatgaaatc gtgaatccac
     6541 agaccggcga ggcccgcaaa acggtggagc tggaccggga gaggctgttc gcgtgcttta
     6601 aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt gaggacgtgg
     6661 aagacaaaga cggcgagcac tgcattatgc caaccagcga gcatctggaa gacatggtgg
     6721 tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg
     6781 cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa aaagccacgt
     6841 actttttcac cggtgaaaat ccgcaatggg aacgggacaa acccacagga atgaagctgt
     6901 gtgattacct ctatcgaaag gtggagcggt tcgccatcga gaacggtgtg caggtggttg
     6961 atgccgggaa gtgctcaccg gagcagatta acgcgatgat tgaggtggtc agcgagcggc
     7021 acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa tataacagcc
     7081 gctattttaa cgtgccggtg cctgaactgc cggaggacaa acccgcccgc cggtggtggc
     7141 cgttcggcaa aaaataacgc cagcaccggc atggccggtg ctgttattct tccccgagct
     7201 tatcccgcat cacctgccag agtgtttccg catcctcgtg aagccgttcg cacatgtcgg
     7261 ccggggtatc catgtccagc tcgttcagct tttccagcaa cagcagcgac tgatccttaa
     7321 tgaatttagc catattgagg gcagtttttt cctgcttcgt catgttctcc attcccttcg
     7381 gtaagttctc agctaaacca gccggaccag aagcgttttt tcttctgtgt cagttctgtt
     7441 gtcaatctgt ccacttcggc ctggagtttc cggcgctcat catcgtgctg gcggcgttct
     7501 tcttcccggg atgttttatc ttccagcatc agggtgacag cctgccggag atcggccagt
     7561 tccttttgca ttgccagaag ctgttcctgt gggaaatttt tttgtgtatt ttcacggtgt
     7621 gaaatgccgg gaatttgttt ctcagggata ttcacatttc cgtatacacg gatcagttct
     7681 gacacatcca cgacagggtt gtttttcccg tcacgtgaca cggtaacctt tccctgtttt
     7741 acatgattgt atagcgttct tctggtaatg cccgcggctt gtgctgcctg tgaaaggttg
     7801 agcaatgttt tcgccactgt atacccctga ttttatcggg tgtgaaaggt gtgtaatttc
     7861 tcacggtaaa tgtatcgctg aattttaccc gtttttccgt tcaggatgtg gttttctgac
     7921 gcgctgcgct tgtcaaaccg gcgacggcca cgcaatgaat tgcgcccccg tcatcggtta
     7981 ttagaggctg tctgcccccg gatacccgga ttctttctga tgcctgacgg tgacgatcac
     8041 aatctggtcc atttcccggt cgtggcggta gagcatcaca tagccactgt cgccaaaccc
     8101 gatcaccagt tccttatatt ccagcggcag aaacggcacc gggcggccaa tgtctggcaa
     8161 tgttttcagt tgctggatag cccggacgat cacctcacct ccttttctgg ctgccagccg
     8221 atttttggtt ttgagaaagt cctgaaggcg ttgtaaatcg cgctgtgcca gcgcggaaat
     8281 cgttacctgt ggcatggcgg agcatcctgc tcgttctcag ttccccaggt acttatccag
     8341 gcttccgctt cttccgcagt aaggtgcaga cccgtttcct gatagtgctg ccatgcggcc
     8401 tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt cgatgtattc ggtgattgcc
     8461 tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg ccagcgtttt cagccggtcc
     8521 ttaagttctt catcgagcct gatggatgtt gctgatgaca taaaagcccc tttgtagtca
     8581 tatgtattac acatgactac tttaatccgt ggcggcgcta tttgccagaa atacggcttt
     8641 tgctgcccat ttttcagctt cgcttccact aatcagggct gctacaggct cactcatcat
     8701 ccaggactat tgcagcctgc gggttaaagg acctgttact ggcggcaacc atcatcattg
     8761 agcttttggg tggtgactgg tcgcggatcc tgtaagccgt catttttcag actgaattca
     8821 aaatttataa cctgtcgcat tgccgggtgc cctgcgggat atagccagtg cctcattgcc
     8881 gtcagcgttt cccgaaaaaa aaggtgacag ccggtaagcg acacgttgtc cactcgtcca
     8941 tcgtccagaa tatcaaacgt ggcggtcacc tgaccttcga tgcgttttgc tgcggcctgt
     9001 accgggtagg ttggctgcag tgtggttacg ggttttagtg gtatcgcatg taccggagtg
     9061 aggaacatgg ccgccagcac tgtcagagta aaagctttca tggtctgaaa tccagagggg
     9121 cgtttttgaa tatttgccag gtattttcat ccggtgccgg cggaattttg gcgtgggtaa
     9181 cggctgatat tgctgcttta cagagtttcg gatctcctcc ttcagcaatg gcgctgttca
     9241 gagtgccatc acgttgtatg gatatccgca cggtgcacat ttttcccgca taggcctctg
     9301 gttcaaacat ctgcatttca acagcggtct tgatggccgc agcgtagcta ctgattttag
     9361 atgacttatc cagagtataa attttcggcg gctgggtggt ggaatgctta caggccgtca
     9421 ggagtaaggg gctgcacaat gccagcagga taaagggtat taaacgcatc gggttgatga
     9481 cctgtcagta aaaaatgtta catgtatatt tgtcactctt tacggccacg ttcatttaca
     9541 taaacacggc ttatcgtatc gtttttacac caaacggccc gccatgcagt tgcgtgcata
     9601 ggcatgcagc gggttacaat ttaccgggcg cgtttccggc ggttttccgg gtggtttgtt
     9661 gcctgtttta tcccgtagcc gccggaaacg ccctcagacc gtctgagcgg tgcgggcaat
     9721 gcgacgttat ggtaaatacc ccttaccggc ggcgctgtgg ctgccaggtg aaggtggttt
     9781 ccgtagcctc gtatttctcc cgacgcattt cctgcgcccg cctggcttca cgttcgtcct
     9841 gaatggcacg aagcaccctt tcccgcacct gttgttttac cgcctttggc agcgttttgt
     9901 tccctttcag gcccatggcc agtgcctgag cgacaaaggc gtccgccagg cgcttttctt
     9961 cagcgggagt cagtttcagg tgcgtcatag tggcctccgg gcgtctcagg tttcccgaac
    10021 agtttaaccg caggtcctct cctcatgcca gttagctttc
//
