LOCUS       NZ_MN689939             4087 bp    DNA     circular CON 26-JUN-2024
DEFINITION  Escherichia coli strain LWY24J plasmid pLWY24J-1, complete sequence.
ACCESSION   NZ_MN689939
VERSION     NZ_MN689939.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Yuan,L. and Li,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) College of Animal Science and Veterinary,
            Henan Agricultural University, Pingan Road, Zhengzhou, Henan
            Province 450046, China
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Newbler software v. v2.6
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009989235.1-RS_2024_06_26
            Annotation Date              :: 06/26/2024 00:54:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MN689939.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LWY24J"
                     /isolation_source="feces"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pLWY24J-1"
                     /geo_loc_name="China"
                     /collection_date="05-Mar-2016"
     gene            complement(join(2761..4087,1..833))
                     /gene="mobQ"
                     /locus_tag="I6U34_RS00005"
     CDS             complement(join(2761..4087,1..833))
                     /gene="mobQ"
                     /locus_tag="I6U34_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_101744408.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTEYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            1012..1266
                     /gene="mobC"
                     /locus_tag="I6U34_RS00010"
     CDS             1012..1266
                     /gene="mobC"
                     /locus_tag="I6U34_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1798..2505
                     /locus_tag="I6U34_RS00015"
     CDS             1798..2505
                     /locus_tag="I6U34_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(476..834)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          835..1011
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1799..2505
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..920)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.673"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2765..4086)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.773"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttcggcaacg cttcgatgat gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcacg
       61 atattcagaa ggtcagccac acggcgggca ggctctgtca gcccgctgtt tctgagttcg
      121 gtacgggaaa tctgtatttc caggttaagt ctgttaatca gacgattctg ttcgccgcgt
      181 tctgtttcga taccgcgtgt ttccatcgca caggcagact ttccgaggtg aataccaggc
      241 gttttctcaa gcccctgtcg ctcgtatgaa cgatggtcta tctcttcctg ataaccggcg
      301 ttcgccagtg ccctgttcgc atggtcagcc catgatgcac gccacgtctc ggcatgtgtc
      361 cggtcgttcc agtccctgac ctttccaccg aatccagcag gtccgacagc tctcgtggtc
      421 agcatgatgt gcgcatgggg atttgtttta tccatgtgat ggaacgccac atcagcaatc
      481 atgccctgac tgacaaagtt ttctctgaca aaggcgagaa cggtttcccg tgcggcatcc
      541 tgcggtagtt cgcgggggat ggcgagttca atttcccgcg caagttgtga gttcttccgg
      601 cgttcggcct tctcaacggc attccagagt tcggcacggt tcgtacacca ggcaggggct
      661 gtcgcgggag tcagaatcac agcttcagcc acaccgcttt tgcgggtgta gtcgtgagtc
      721 agtccggtac gctcatcaag catcagtgaa ccggaacgat aggcagcaga agcaacggcg
      781 ctgtagccgc tgtttctgga gatgattttc atgctgagat gataaatcgc catttctgcc
      841 gtaatgcctt tttgtggttt tctttcagcc ccgcagggcg cacacaatcg aaacggagtg
      901 gagattgtat aagtgcgcac ttcgtgattt tccgtgcatt atgccagctc agtttttaaa
      961 ctaccagtga cgcgctaaaa tgcgattttt catcgcgcac ggagttacat catgagcaga
     1021 acactcgaac agaagattgc tgatgctgag gccagattac agcgcctgaa ggcgaagagc
     1081 aggagtctgg acacagcgca gaaggtcatt gtgggtgcag cattgctggc aaaggtcaga
     1141 aagccggagg aggtgcagtt gcgtgcctgg ttactccagt tcctgaaggc agaggtgaca
     1201 cgacaggctg atgtgacgcg catactgccg ctgattaacg agctggaagc gctgccagga
     1261 cagtgattgt atcgtccgct cgccgcagtc ccagcgaccg agcgttagcg agcgagtggg
     1321 cgaggaagcg gaagtgaatc cgtcgcctga ttgagcacat acgcacggtt tttcgggagg
     1381 tgatatgcgc tgaacccgca tgaatactgg atttccacag ggttggaggg ggtgtattcg
     1441 gaacttgcaa ttctgctttt ttgcaacgca ttgtttttat tggttttttg ttttcagcac
     1501 tccaagttat ccacaggctc gtcgttaagt ttttagatct tgtttttaag atcttgtttt
     1561 taatacttgt tttagcgcca aaattcctta tagaaaacaa aatgttacaa cgctataggt
     1621 gatatgattt aaggtaaagg tgatataatt taaggtgctt gggtgaggta atttaaggta
     1681 aaggtgagga gatttaaggt aatattcaca ggaaaatggg gggtgaaaat ataattgctc
     1741 accaatgcta aaggtgatag gtttttattt cacctcattt ttggcctggt ggttaaaatg
     1801 acatcaaacc ctctgatagc ctataagtcc aatgcgctgg ttgaggccag ttacaagcta
     1861 actttacaag aacagcgttt cttattgctc tgcataagcc gacttaactc cggtactgac
     1921 gtagcttcac ctgaattgca aaagacaatg accattacag ctgcggaata ttttgattca
     1981 ttccctgata tggggcggaa aaatgccgag gttcagctac aggaagcgat cgatcgtttg
     2041 tgggacaggt cgatcattct caaggacgac gagaagcgcg aggagttccg ctggattcag
     2101 tatcgggcac agtacgcgag gggagagggg aaagcacaaa tcactttttc tgacgcggta
     2161 atgccttatc tgacacagtt gaaggggcaa ttcaccagag tcgtcattaa aaatatttct
     2221 aatttatcaa gaagttacag cataagaata tacgaaattc tccagcaatt ccgcagtact
     2281 ggggaacgta ttatcgccct cgatgatttt aaatcatcac tgatgcttga tggtaaatat
     2341 aaagatttca aaacgttaaa cagggatcta ataaaaccct gtgttgacga actgaacaaa
     2401 aaaagcgacc tggctgtaac ggttgagacg atcaaaaagg ggcgcacggt tgtagccctg
     2461 cattttcgct tcaaggaaga caaacagatc aagatgacga tctagaatcg attttaaggc
     2521 acctaaaacg ctccctgctg cgtttttttt gtgttagtag tgcgaaggta ctattgaagt
     2581 gaaaaattgc gtcaaaagcg ctctaatgca gcgaaaaaag aacccccatt tttcgggggc
     2641 ggaaaaacaa aactatgctt gcattatgtg tgtgcttctt gcattcatcc ctgtcgatga
     2701 acatcgaaga acatcgacga acataacacg atgttaaaca aaacaaaata ttgttcacat
     2761 tcactgtgac caaccgcgat catgtctcct ctcggtggcg tgtgttcgtg catcttcctc
     2821 gcgccgccat tcgtcccagg tacgattaaa acccagcgag cggaacagat gatccggctg
     2881 tttttctggt gtgttgacgg cagcaaaaag cggtgcctca cgggttttca gatgaaactc
     2941 cataagttcc ggcgcaggtg atatgtggct gtctgcatcg tgatttacgg caacaccata
     3001 ccctgcatac cgtaacccct tatcggtcag tgcactgatg accagctggc tgttatcggc
     3061 tgagggaacg gcaaacgcgg gatacagcgt accattaagc cggacaacac cgcccagaga
     3121 gtcatccacg cccatgacca gcaaaagctg aagctggcgg gtttcccggt cgacagaact
     3181 cattaccggc atcagcgtcg gtagcagacg tccctgcgta aaatccacct tccttgctgc
     3241 cagtgatggc atggctggct ggcggtcttc tccaagacag aaagactccg gccatgtctt
     3301 cggctggcgg agattgcggg tatattgcgg ccatgtctgg caaacctgcg tcagaagttg
     3361 ctcaaaggtc gcagaggaat aaacacgttg cagtggtgga tgttgaccga tttcgcttcg
     3421 gtcacagata taaccactcc tgtcacgcat taaatcaaca cgctcaaaga ttctggtgcg
     3481 gtatttctcc agctcgttcc cccagagcgt gtattctccg ttccggttgt gaagcagaag
     3541 gtaacaggtc atttccccgt taccaggcat gggggcctcg ccatactccg taacgactgc
     3601 gctcagtacc cctgaatcca taacggcatt gtcatttttc ctgagctgtt ccgctcgtcg
     3661 ctccgcgcga ctacgttctg tatcctcctg ctccttccgg cgcaattcct cttcacgttt
     3721 aagcaactgg actctgagag ctgcaaggcc ggatgccccc cactgctgcc actgtttttc
     3781 aaatgccgtc tgactcgcgg gaatgtcctg tttctccacc ttccggtaat gctgtcgcaa
     3841 tttttccatt tgctggctca gcgactggat ttttccctgt tcgctacggt attccggtgc
     3901 cgcccattgc atcaggggaa tacgggaaaa accggacgaa aacggcgatg attttttcag
     3961 gctggcagca tggctcatcg cttcctttcg ttcggtgttc agctcttccc agcgtctgcg
     4021 cgtcgcctgc atatctgcca tcatcgacat ggcattactg gtgagtttcc atgccgcatt
     4081 actgtcc
//
