LOCUS       NZ_MN612109             4439 bp    DNA     circular CON 25-MAY-2024
DEFINITION  Staphylococcus pseudintermedius strain G3C4 plasmid pSP-G3C4,
            complete sequence.
ACCESSION   NZ_MN612109
VERSION     NZ_MN612109.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14228847
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Vines Pujol,J., Francino Marti,O. and Cusco Marti,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2019) Departament de Ciencia Animal i dels
            Aliments, Universitat Autonoma de Barcelona, Travessera dels Turons,
            s/n, Bellaterra, Barcelona 08193, Espana
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.6
            Assembly Name                :: pSP-G3C4
            Coverage                     :: 64X
            Sequencing Technology        :: Illumina; Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009989225.1-RS_2024_05_25
            Annotation Date              :: 05/25/2024 02:50:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MN612109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus pseudintermedius"
                     /mol_type="genomic DNA"
                     /strain="G3C4"
                     /isolation_source="skin, otitis"
                     /host="dog"
                     /db_xref="taxon:283734"
                     /plasmid="pSP-G3C4"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            70..1311
                     /gene="mobV"
                     /locus_tag="HTX60_RS00005"
     CDS             70..1311
                     /gene="mobV"
                     /locus_tag="HTX60_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            1838..2782
                     /locus_tag="HTX60_RS00010"
     CDS             1838..2782
                     /locus_tag="HTX60_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2944..4323
                     /gene="tet(K)"
                     /locus_tag="HTX60_RS00015"
     ARG             2945..4320
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1839..2782
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          71..1308
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taagtctagt gtattagact taaactatta aatacacatg aaacctttgt gcttaggagt
       61 gatttttata tgtcttattc cattgttaga gtttcaaaag ttaaatctgg aacaaataca
      121 acgggcatac aaaaacatgt tcaaagagaa aataataatt atgaaaatga agatatagac
      181 catagtaaaa cttacttaaa ttatgatttg gtaaatgcta ataaacagaa ttttaataac
      241 ttgattgatg aaaaaatcga acagaattat acaggcaaaa gaaaaattag aacagacgcg
      301 attaaacaca ttgatggttt aattacatca gacaatgatt tctttgataa tcaaacgcca
      361 gaagatacaa agcagttttt tgaatatgct aaagagtttt tagaacaaga atacggtaaa
      421 gataatttat tatatgcaac agttcacatg gacgaaaaaa caccacatat gcattatggc
      481 gttgttccaa taactgatga tggtcgttta agtgctaaag aagttgtagg taataaaaaa
      541 gctttaacag cgtttcaaga tagatttaat gagcatgtta aacaacgagg atatgattta
      601 gaacgtgggc aatcaagaca agtaacaaat gctaaacatg agcaaataag tcagtataaa
      661 caaaaaacag aatatcataa gcaagaatat gaacgtgaga gccaaaaaac agaccatata
      721 aagcaaaaga acgataaatt aatgcaagag taccaaaaat cgttaaatac gcttaaaaag
      781 cctataaatg ttccgtatga gcaagaaact gaaaaagtag gtggtttatt tagcaaagaa
      841 atacaagaaa ctggaaatgt tgtaataagc caaaaagatt tcaatgaatt tcagaaacag
      901 ataaaagctg ctcaagatat ttcggaagat tacgagtata taaagtctgg tagagcctta
      961 gatgataaag ataaggaaat acgagagaaa gatgatttat taaataaagc agttgagcgt
     1021 attgaaaacg cagacgataa ttttaaccaa ctttacgaaa atgcaaagcc acttaaagag
     1081 aatatagaaa tagcgttaaa gcttttaaaa atcttactaa aagagttaga acgagtttta
     1141 ggaagaaata cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat
     1201 ggtttagcag gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag
     1261 caacaagaac aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat
     1321 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
     1381 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
     1441 ctgtaggttc cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga
     1501 aattcagaga agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag
     1561 ttttaactgt ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga
     1621 ccaagtcttt tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga
     1681 ccaaagtttg tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg
     1741 tatatttaga tattaaacga tatttaaata tacatcaaga tgtatatttg ggtgagcgat
     1801 tccttaaacg aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa
     1861 tcattcaaat catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa
     1921 tagccggttg gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat
     1981 gacgatcgtt ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt
     2041 agagccccaa ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca
     2101 agaaaaagtt tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat
     2161 gcgtattgaa tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa
     2221 tataataagc tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga
     2281 agatgatttg agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta
     2341 tggtcgtaat ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat
     2401 tagaatttat aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga
     2461 acatttatgg cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg
     2521 ctttagtgat ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag
     2581 agcaatagtt tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc
     2641 tagaacaaaa tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat
     2701 gaaatcgact ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca
     2761 tgaatttaaa ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt
     2821 aaactattct aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt
     2881 tatatgaggt gaaagaataa tttaccccta taaactttag tcacctcaag taaagaggta
     2941 aaattgttta gtttatataa aaaatttaaa ggtttgtttt atagcgtttt attttggctt
     3001 tgtattcttt cattttttag tgtattaaat gaaatggttt taaatgtttc tttacctgat
     3061 attgcaaatc attttaatac tactcctgga attacaaact gggtaaacac tgcatatatg
     3121 ttaacttttt cgataggaac agcagtatat ggaaaattat ctgattatat aaatataaaa
     3181 aaattgttaa ttattggtat tagtttgagc tgtcttggtt cattgattgc ttttattggt
     3241 cacaatcact tttttatttt gatttttggt aggttagtac aaggagtagg atctgctgca
     3301 ttcccttcac tgattatggt ggttgtagct agaaatatta caagaaaaaa acaaggcaaa
     3361 gcctttggtt ttataggatc aattgtagct ttaggtgaag ggttaggtcc ttcaataggg
     3421 ggaataatag cacattatat tcattggtct tacctactta tacttcctat gattacaata
     3481 gtaactatac cttttcttat taaagtaatg gtacctggta aatcaacaaa aaatacatta
     3541 gatatcgtag gtattgtttt aatgtctata agtattatat gttttatgtt atttacgaca
     3601 aattataatt ggactttttt aatactcttc acaatctttt ttgtgatttt tattaaacat
     3661 atttcaagag tttctaaccc ttttattaat cctaaactag ggaaaaacat tccgtttatg
     3721 cttggtttgt tttctggtgg gctaatattt tctatagtag ctggttttat atcaatggtg
     3781 ccttatatga tgaaaactat ttatcatgta aatgtagcga caataggtaa tagtgttatt
     3841 tttcctggaa ccatgagtgt tattgttttt ggttattttg gtggtttttt agtggataga
     3901 aaaggatcat tatttgtttt tattttagga tcattgtcta tctctataag ttttttaact
     3961 attgcatttt ttgttgagtt tagtatgtgg ttgactactt ttatgtttat atttgttatg
     4021 ggcggattat cttttactaa aacagttata tcaaaaatag tatcaagtag tctttctgaa
     4081 gaagaagttg cttctggaat gagtttgcta aatttcacaa gttttttatc agagggaaca
     4141 ggtatagcaa ttgtaggagg tttattgtca ctacaattga ttaatcgtaa actagttctg
     4201 gaatttataa attattcttc tggagtgtat agtaatattc ttgtagccat ggctatcctt
     4261 attattttat gttgtctttt gacgattatt gtatttaaac gttctgaaaa gcagtttgaa
     4321 tagttatatt atattttggt ttagaactat gagtggctag cattttgcca ctcatttttt
     4381 gcgttagcaa aaacaggttt aagcctcgca gagcacacgt attaacgact tattaaaaa
//
