LOCUS       NZ_MK181634            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae plasmid unnamed, complete sequence.
ACCESSION   NZ_MK181634
VERSION     NZ_MK181634.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227910
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Qin,S., Cheng,J. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2018) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: CLC Genomics Workbench v. 10.0
            Sequencing Technology        :: Illumina; minION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/09/2024 02:36:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MK181634.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolation_source="Sputum"
                     /host="Homo sapiens male"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="China"
                     /note="20171110"
     gene            join(8382..10060,1..286)
                     /locus_tag="HTU60_RS00005"
     CDS             join(8382..10060,1..286)
                     /locus_tag="HTU60_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            286..1017
                     /gene="mobC"
                     /locus_tag="HTU60_RS00010"
     CDS             286..1017
                     /gene="mobC"
                     /locus_tag="HTU60_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            1024..1554
                     /locus_tag="HTU60_RS00015"
     CDS             1024..1554
                     /locus_tag="HTU60_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1581..1760)
                     /locus_tag="HTU60_RS00020"
     CDS             complement(1581..1760)
                     /locus_tag="HTU60_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1786..2214)
                     /locus_tag="HTU60_RS00025"
     CDS             complement(1786..2214)
                     /locus_tag="HTU60_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2378..2692)
                     /locus_tag="HTU60_RS00030"
     CDS             complement(2378..2692)
                     /locus_tag="HTU60_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2680..2958)
                     /locus_tag="HTU60_RS00035"
     CDS             complement(2680..2958)
                     /locus_tag="HTU60_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3133..3498)
                     /locus_tag="HTU60_RS00070"
     CDS             complement(3133..3498)
                     /locus_tag="HTU60_RS00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(3495..3866)
                     /gene="tolA"
                     /locus_tag="HTU60_RS00045"
     CDS             complement(3495..3866)
                     /gene="tolA"
                     /locus_tag="HTU60_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(4140..4385)
                     /locus_tag="HTU60_RS00050"
     CDS             complement(4140..4385)
                     /locus_tag="HTU60_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4712..6397
                     /locus_tag="HTU60_RS00055"
     CDS             4712..6397
                     /locus_tag="HTU60_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6407..6664
                     /locus_tag="HTU60_RS00060"
     CDS             6407..6664
                     /locus_tag="HTU60_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            6749..6898
                     /locus_tag="HTU60_RS00065"
     CDS             6749..6898
                     /locus_tag="HTU60_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7002..7580)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          287..1014
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ggaggcgggg aacgtggagc acatcagcgg cgacagcttc atccagaagg aggcgcggcc
       61 gctgtttgac gtgaacaccc tgcaacacct gcctgacggg ttcgcggtga tgaccgggct
      121 gggcgtggcg cggctggcgt tcagcagccc gctgcgggtt gagcgccggg aaatttcgct
      181 gaaaaccttc ccggtgctgg cgaaaacgga cccgctggcg gagtacctgc ctccggccga
      241 cggcggcgga ccaaaagcgg atgatgattt tgcggggctt tactgatgac actggagcag
      301 tacaacgtca gccacgcgaa gcggcaggcg cggaacgcgg agaaaacacg gctgaccctg
      361 cgctggctgc gggaagagct gtgttccacg gcggagctgg ttgcgcgacg gctggggatt
      421 gcggcggtac agccggttta ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc
      481 cgggcgaaat acccggttga cggccgtcag gtcagcgtgt gggggctgac gccgcacggg
      541 gtggcgttct cgttcgacga ggacgagccg ctgacggacg ttattccgtt tcagccgtca
      601 agggtgtcgg cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg cctctccatg
      661 gagggccggg gggccaccgg ctggcggtac ctgcaccgta tggccctgaa agggatgaaa
      721 gtgccggacg cgctggcgga gctggacggc agaacggtgg catttgaggt agagcgcacc
      781 gtcaagagcc gcaggcggta tcaggaagtg gtgtccgggt atctgttcaa ccgccgggcg
      841 aacggcatcg atgaaatctg gtacatctgc cctgaccggc cgacgcttgt ccgggtacag
      901 cgggcgattc tggcggtgga tgaaatcgtg aatccacaga ccggcgaggc ccgcaaaacg
      961 gtggagctgg accgggagag gctgttcgcg tgctttaaat ttatgacaac ggagtgaggg
     1021 attatggcgc tgtacatggt atttattgag gacgtggaag acaaagacgg cgagcgctgc
     1081 attatgccaa ccagcgagca tctggaagac atggtggtgc tcgcctggca gaccggggcg
     1141 tatgttttct gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg
     1201 tatgacgagc ggctcaacag cgacgaaaaa gccacgtact ttttcaccgg tgaaaatccg
     1261 caatgggaac gggacaaacc cacaggaatg aagctgtgtg attacctcta tcgaaaggtg
     1321 gagcggttcg ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag
     1381 cagattaacg cgatgattga ggtggtcagc gagcggcacg gggaaagcgc ggtgaacgag
     1441 aagctgaaag cggagctgat gcgggaatat aacagccgct attttaacgt gccggtgcct
     1501 gaactgccgg aggacaaacc cgcccgccgg tggtggccgt tcggcaaaaa ataacgccag
     1561 caccggcatg gccggtgctg ttattcttcc ccgagcttat cccgcatcac ctgccagagt
     1621 gtttccgcat cctcgtgaag ccgttcgcac atgtcggccg gggtatccat gtccagctcg
     1681 ttcagctttt ccagcaacag cagcgactga tccttaatga atttagccat attgagggca
     1741 gttttttcct gcttcgtcat gttctccatt cccttcggta agttctcagc taaaccagcc
     1801 ggaccagaag cgttttttct tctgtgtcag ttctgttgtc aatctgtcca cttcggcctg
     1861 gagtttccgg cgctcatcat cgtgctggcg gcgttcttct tcccgggatg ttttatcttc
     1921 cagcatcagg gtgacagcct gccggagatc ggccagttcc ttttgcattg ccagaagctg
     1981 ttcctgtggg aaattttttt gtgtattttc acggtgtgaa atgccgggaa tttgtttctc
     2041 agggatattc acatttccgt atacacggat cagttctgac acatccacga cagggttgtt
     2101 tttcccgtca cgtgacacgg taacctttcc ctgttttaca tgattgtata gcgttcttct
     2161 ggtaatgccc gcggcttgtg ctgcctgtga aaggttgagc aatgttttcg ccactgtata
     2221 cccctgattt tatcgggtgt gaaaggtgtg taatttctca cggtaaatgt atcgctgaat
     2281 tttacccgtt tttccgttca ggatgtggtt ttctgacgcg ctgcgcttgt caaaccggcg
     2341 acggccacgc aatgaattgc gcccccgtca tcggttatta gaggctgtct gcccccggat
     2401 acccggattc tttctgatgc ctgacggtga cgatcacaat ctggtccatt tcccggtcgt
     2461 ggcggtagag catcacatag ccactgtcgc caaacccgat caccagttcc ttatattcca
     2521 gcggcagaaa cggcaccggg cggccaatgt ctggcaatgt tttcagttgc tggatagccc
     2581 ggacgatcac ctcacctcct tttctggctg ccagccgatt tttggttttg agaaagtcct
     2641 gaaggcgttg taaatcgcgc tgtgccagcg cggaaatcgt tacctgtggc atggcggagc
     2701 atcctgctcg ttctcagttc cccaggtact tatccaggct tccgcttctt ccgcagtaag
     2761 gtgcagaccc gtttcctgat agtgctgcca tgcggcctgt ccgtcccgca ggtactggct
     2821 gcgcttctct tcccggtcga tgtattcggt gattgcctcc agcattagcg cgtgcgctga
     2881 gcggtgccgg tcatcggcca gcgttttcag ccggtcctta agttcttcat cgagcctgat
     2941 ggatgttgct gatgacataa aagccccttt gtagtcatat gtattacaca tgactacttt
     3001 aatccgtggc ggcgctattt gccagaaata cggcttttgc tgcccatttt tcagcttcgc
     3061 ttccactaat cagggctgct acaggctcac tcatcatcca ggactattgc agcctgcggg
     3121 ttaaaggacc tgttactggc ggcaaccatc atcattgagc ttttgggtgg tgactggtcg
     3181 cggatcctgt aagccgtcat ttttcagact gaattcaaaa tttataacct gtcgcattgc
     3241 cgggtgccct gcgggatata gccagtgcct cattgccgtc agcgtttccc gaaaaaaaag
     3301 gtgacagccg gtaagcgaca cgttgtccac tcgtccatcg tccagaatat caaacgtggc
     3361 ggtcacctga ccttcgatgc gttttgctgc ggcctgtacc gggtaggttg gctgcagtgt
     3421 ggttacgggt tttagtggta tcgcatgtac cggagtgagg aacatggccg ccagcactgt
     3481 cagagtaaaa gctttcatgg tctgaaatcc agaggggcgt ttttgaatat ttgccaggta
     3541 ttttcatccg gtgccggcgg aattttggcg tgggtaacgg ctgatattgc tgctttacag
     3601 agtttcggat ctcctccttc agcaatggcg ctgttcagag tgccatcacg ttgtatggat
     3661 atccgcacgg tgcacatttt tcccgcatag gcctctggtt caaacatctg catttcaaca
     3721 gcggtcttga tggccgcagc gtagctactg attttagatg acttatccag agtataaatt
     3781 ttcggcggct gggtggtgga atgcttacag gccgtcagga gtaaggggct gcacaatgcc
     3841 agcaggataa agggtattaa acgcatcggg ttgatgacct gtcagtaaaa aatgttacat
     3901 gtatatttgt cactctttac ggccacgttc atttacataa acacggctta tcgtatcgtt
     3961 tttacaccaa acggcccgcc atgcagttgc gtgcataggc atgcagcggg ttacaattta
     4021 ccgggcgcgt ttccggcggt tttccgggtg gtttgttgcc tgttttatcc cgtagccgcc
     4081 ggaaacgccc tcagaccgtc tgagcggtgc gggcaatgcg acgttatggt aaatacccct
     4141 taccggcggc gctgtggctg ccaggtgaag gtggtttccg tagcctcgta tttctcccga
     4201 cgcatttcct gcgcccgcct ggcttcacgt tcgtcctgaa tggcacgaag caccctttcc
     4261 cgcacctgtt gttttaccgc ctttggcagc gttttgttcc ctttcaggcc catggccagt
     4321 gcctgagcga caaaggcgtc cgccaggcgc ttttcttcag cgggagtcag tttcaggtgc
     4381 gtcatagtgg cctccgggcg tctcaggttt cccgaacagt ttaaccgcag gtcctctcct
     4441 catgccagtt agctttcaga tgtgtctata ccatctgaaa tgtaagtgtg atgaatgtct
     4501 taaataataa aatttgttgc catgagaaat tttcgggaaa agacgcgatc tgcttacagg
     4561 tggctccggg cgcttttttg tgcctgaaga tgagaactaa tctcatatat tgacacctga
     4621 aaacgggagg agtaaggtaa taattatact gtgtatatgt acagtatgtt ggctttaatt
     4681 tattgtttta aatgtcaaag aggaaaacgg tatgagtggt ggagacggtc gaggtccggg
     4741 taattcaggt ctgggacata atggtggtca ggccagtggg aatgtgaacg gtacgtctgg
     4801 taaaggtggc ccttcatcag gtggtggtac ggatccaaac agcgggccgg gctggggtac
     4861 gacgcatacg ccgaatggag atattcataa ctataatccg ggggagtttg gtaacggcgg
     4921 gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat agcggtagct ctggtggcgg
     4981 acagtcttcg gccactgcga tggccttcgg tctgcctgcc ctggctactc cgggggctga
     5041 aggactggct ttatcagttt ccggcgatgc gttgtcggcc gccgttgctg atgtgctggc
     5101 tgccctgaag gggccgttta agtttggtct gtgggggatt gcgatctacg gtgtgctgcc
     5161 ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa attatgacgt cattaccggc
     5221 cgatgcggtg acggagactc cggcaagtac tttaccactg gaccaggcga cggttcgtgt
     5281 cagacaacgg gttgtggatg tggtgaagga tgagcggcag catattgcgg ttgtcgcagg
     5341 tcggccaatg agtgtccctg tggtggatgc gaaaccgaca aaacgtccgg gggtattcag
     5401 tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta ccgaaaggtg ttccgacagc
     5461 gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat tcacgtccgg ctggttttac
     5521 agccggtggt aactcccgtg aggccgttat tcgtttcccg aaagagaccg gacagaagcc
     5581 ggtttatgtg tcggtgacag atgttcttac cccggcacag gtaaaacagc gtcaggagga
     5641 agaaaagcgt cgccagcagg catgggacgc cgctcatccg gaagaggggc tgaaaagaga
     5701 ctatgataaa gcgaaagctg agctggatgc cgaagataaa aatattgcga ccttaaacag
     5761 ccgcattgca tcgacagaga aggcgctccc cggtgcaagg gctgctgttc aggaagccga
     5821 taaaaaggta aaagaggcag aggcaaataa agatgatttt gtgacttata acccgcctca
     5881 tgaatatggc tccgggtggc aggatcaggt tcgctatctt gataaggata ttcagaatca
     5941 gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca atgaatgaat ccttatccag
     6001 agataaggct gcacttaccg gggcgatgga gagccggaaa caaaaggaga aaaaagcgaa
     6061 ggatgcagaa aataagttaa atgaggaaaa gaacaagcct cgcaagggga ctaaagacta
     6121 cggccatgat tatcatccag tacctaaaac agaagacata aagggacttg gagagttaaa
     6181 gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt aagcgggccc gatggtatgg
     6241 cgataaaaaa cgtaaaattt atgaatggga ttcccagcac ggtgagcttg aagggtatcg
     6301 tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa acgggtaagc aggttaaagg
     6361 gccagatcca aaacggaaca ttaaaaaata tctttgagag gtaaatatgg ggcttaaatt
     6421 acgtttgaca tggtttgata agaaaactga agagtttaaa ggggaagagt attctaaaga
     6481 ctttggtgac gatggttctg tcattgaaag cttgggaatg cctttaaagg ataatattaa
     6541 taatggtgag tttgatttga agaaagaatg ggttccttta ctacagccat tttttaaaaa
     6601 tcaaataaaa cctgatgtgg ataattgtta tatagctttt gattatcggg acggtaactg
     6661 gtaacaaaaa attatcaagg atgatttgaa atactgactt ccggtgttca gggatgaatg
     6721 ccggagtctt taaatacagg gaaagtatat gaaaaaggta aagactattt ttctttttat
     6781 attgattgtt gctggctttc ttctggccgc atgtcaggca aattatatcc gggatgttca
     6841 gggcggaacg gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg ttcagtagaa
     6901 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg ccctgtaaac
     6961 gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg aactgcttaa
     7021 ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag tgtagccgga
     7081 ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc tgcgaactcc
     7141 cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt tcccgggttg gactcaagtg
     7201 aacagttacc ggataaggcg cagcagtcgg gctgaacggg gagttcttgc ttacagccca
     7261 gcttggagcg aacgacctac accgagccga gataccagtg tgtgagctat gagaaagcgc
     7321 cacacttccc gcaagggaga acggcggaca ggtatccggt aaacggcagg gtcggaacag
     7381 gagagcgcaa gagggagcga cctaccggaa acggtgggga tctttaagtc ctgtcgggtt
     7441 tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc accgaaaaag
     7501 cgtctgttta tgtgaactct ggcaggaggg cggagcctat ggaaaaacgc caccggcgcg
     7561 gccctgccgt tttgcctcac atgttagtcc cctgcttatc cacggaatct gtgggtaact
     7621 ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg agcgtagcga ccgagtgagc
     7681 gaggaggcgc aatttccccg gcacctgcaa gacggtcacc ggacgcgctg atgtgggata
     7741 ttgtcccctg attaagtctt tctattccct gacgatcaat tatcaggata ggtgaaccat
     7801 gtcggttttt cgctgaacat tttacgttaa cctatctctg ttgctttttt ctccttgcgg
     7861 agaaatgcaa gtcatccccg ctggggcgtg gctgacttca ggggaaaaga ccttgcttac
     7921 gctggctgga cgccaccgac gcacggtctt ttcccctctt tctggctctg tccggctgga
     7981 cgccggccac gccagaaatt cacccggccc ccgtcaggct cacgcctgac cacccacaaa
     8041 aatccacggt gctggacgca cctgacgaag cagcaaagct gctcctgatt ttgcgggtgg
     8101 tcagccgctc gctatggcga actctgacgg gggccggaac accggaaaag accgcactgg
     8161 ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt gttcctccgg ggctgcgcct
     8221 tttccgcatg gggcggaaag aggctggcct ccgcggtgaa agctgaacgc aatcaccacg
     8281 gacgtgaccg ccgccgccat gccttggggc atgtccgcgg cttgtgcagc gggggctgcg
     8341 catcacgccg gtaaacccgt cgtctgaagg agagagattg catgtttaat acggattcgc
     8401 ttgcctggca gtggcatatc ccggagctgg cccggcagat ttacccgtgg gtcagctggc
     8461 tgtaccggga gtattacagt ctggccggtt tttttctggt tatcggcctg tttttcggtc
     8521 tggctgaact ctgggccggc cgaatgcgtt ttgtggggct gagccataag gcgcggcggg
     8581 agtatgccga tcgctataag cgcgataata accggactgt gccattttcg gtgctggcct
     8641 cgctggattc acccgggcgg atggtcaggg cacggcgaca tgtctggaac tacgcgtact
     8701 ggctgaccgg cgctgccggt ctggcccttc tgggggggct ggttgtcgcc gtgtttatcg
     8761 agagctgggg agaaaccgtc ctgcgcccgg acctctatgc gaacgcagat tattacctgc
     8821 tgcagccggc taacctgttc gggatagcct ggtgtgccgg agggcttgtc gccggcgtcc
     8881 ttctggcgcg gctgctggcg ttcctgctgc ttgatggtca ttttgcggcc gctgacgagg
     8941 cggtgaacgc gaaactgaat caggagagcc ggcgcagcag ccagcggacc ggagaaatga
     9001 cagacgtccg ccacctgcat ttcggggagc cggtaccggt taacgcgctg gccgattttt
     9061 ccacggagca ggcccggaaa cagcaggcgg tgtttctggg taaggatgag cagggccagc
     9121 cggtgctggt ccctcgtgat acctggcgca agaccaatat tcagatcctc ggcctgcccg
     9181 ggagcggcaa gagcgcgatg gccaccaacg cgctcattcg ctgcgtacgc gatttcgggg
     9241 acgctgtggt gtacttcgac cccaaagggg acgcgtgggc gccgcacgtc ttccgggcgc
     9301 actgcccgga cttcaccctg ctggatctgc ggccggggaa accggcgcag ctgaatctgt
     9361 tccgcgatct cgaccagtac gccctgaaga atctgctggt tgccgggttt aacctgagtg
     9421 agaccggcga cgtggcggac cactaccgca tcagcgagca gaaagcggcg aagctgattg
     9481 cggagcagtt cccgcagggg gcaaacattc agcaggtgct ggcggcggcg tacgcgttgc
     9541 cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa gctggagaac gtggccgacc
     9601 tgagcgtcct gcagacggac agcggtatcg atgtggcggg gataatcaac ggcggcggct
     9661 gcctgtacgt catcggctcg atggacgatg aggcggtgat ccgggtgcag aagatgctgt
     9721 ttgcccgctg cgcgcaaatc atcattgcgc gggatgagtt ccggaggtgg ccgcacgcga
     9781 gcatcatgct cgatgagatt aaatatctgc tgtcgaagta cgtgctgaac gcgctgggga
     9841 cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca gtcgctgggc gacttcggtc
     9901 agtgcgggca ggatctgccc gctgattttg tgaaaaccac ggtactggat aacacgccga
     9961 tccgctggtt ctatcgcgcg gccagccagg agtccgcgca gtgggcggcg ggccagaccg
    10021 gggagatccg ggtggacgtg gagcgccgcc gggccagccg
//
