LOCUS       NZ_MK181632            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae plasmid unnamed, complete sequence.
ACCESSION   NZ_MK181632
VERSION     NZ_MK181632.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227912
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Qin,S. and Cheng,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2018) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: CLC Genomics Workbench v. 10.0
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/09/2024 02:31:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MK181632.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolation_source="Blood"
                     /host="Homo sapiens male"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="China"
                     /note="20171103"
     gene            join(9881..10060,1..66)
                     /locus_tag="HTU51_RS00005"
     CDS             join(9881..10060,1..66)
                     /locus_tag="HTU51_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            340..711
                     /gene="tolA"
                     /locus_tag="HTU51_RS00010"
     CDS             340..711
                     /gene="tolA"
                     /locus_tag="HTU51_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            708..1073
                     /locus_tag="HTU51_RS00070"
     CDS             708..1073
                     /locus_tag="HTU51_RS00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1248..1526
                     /locus_tag="HTU51_RS00020"
     CDS             1248..1526
                     /locus_tag="HTU51_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1514..1828
                     /locus_tag="HTU51_RS00025"
     CDS             1514..1828
                     /locus_tag="HTU51_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1992..2420
                     /locus_tag="HTU51_RS00030"
     CDS             1992..2420
                     /locus_tag="HTU51_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2446..2625
                     /locus_tag="HTU51_RS00035"
     CDS             2446..2625
                     /locus_tag="HTU51_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2652..3182)
                     /locus_tag="HTU51_RS00040"
     CDS             complement(2652..3182)
                     /locus_tag="HTU51_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3189..3920)
                     /gene="mobC"
                     /locus_tag="HTU51_RS00045"
     CDS             complement(3189..3920)
                     /gene="mobC"
                     /locus_tag="HTU51_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3920..5884)
                     /locus_tag="HTU51_RS00050"
     CDS             complement(3920..5884)
                     /locus_tag="HTU51_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7368..7517)
                     /locus_tag="HTU51_RS00055"
     CDS             complement(7368..7517)
                     /locus_tag="HTU51_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7602..7859)
                     /locus_tag="HTU51_RS00060"
     CDS             complement(7602..7859)
                     /locus_tag="HTU51_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7869..9554)
                     /locus_tag="HTU51_RS00065"
     CDS             complement(7869..9554)
                     /locus_tag="HTU51_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             2869..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6687..7265
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3193..3920)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgcgtcggg agaaatacga ggctacggaa accaccttca cctggcagcc acagcgccgc
       61 cggtaagggg tatttaccat aacgtcgcat tgcccgcacc gctcagacgg tctgagggcg
      121 tttccggcgg ctacgggata aaacaggcaa caaaccaccc ggaaaaccgc cggaaacgcg
      181 cccggtaaat tgtaacccgc tgcatgccta tgcacgcaac tgcatggcgg gccgtttggt
      241 gtaaaaacga tacgataagc cgtgtttatg taaatgaacg tggccgtaaa gagtgacaaa
      301 tatacatgta acatttttta ctgacaggtc atcaacccga tgcgtttaat accctttatc
      361 ctgctggcat tgtgcagccc cttactcctg acggcctgta agcattccac cacccagccg
      421 ccgaaaattt atactctgga taagtcatct aaaatcagta gctacgctgc ggccatcaag
      481 accgctgttg aaatgcagat gtttgaacca gaggcctatg cgggaaaaat gtgcaccgtg
      541 cggatatcca tacaacgtga tggcactctg aacagcgcca ttgctgaagg aggagatccg
      601 aaactctgta aagcagcaat atcagccgtt acccacgcca aaattccgcc ggcaccggat
      661 gaaaatacct ggcaaatatt caaaaacgcc cctctggatt tcagaccatg aaagctttta
      721 ctctgacagt gctggcggcc atgttcctca ctccggtaca tgcgatacca ctaaaacccg
      781 taaccacact gcagccaacc tacccggtac aggccgcagc aaaacgcatc gaaggtcagg
      841 tgaccgccac gtttgatatt ctggacgatg gacgagtgga caacgtgtcg cttaccggct
      901 gtcacctttt ttttcgggaa acgctgacgg caatgaggca ctggctatat cccgcagggc
      961 acccggcaat gcgacaggtt ataaattttg aattcagtct gaaaaatgac ggcttacagg
     1021 atccgcgacc agtcaccacc caaaagctca atgatgatgg ttgccgccag taacaggtcc
     1081 tttaacccgc aggctgcaat agtcctggat gatgagtgag cctgtagcag ccctgattag
     1141 tggaagcgaa gctgaaaaat gggcagcaaa agccgtattt ctggcaaata gcgccgccac
     1201 ggattaaagt agtcatgtgt aatacatatg actacaaagg ggcttttatg tcatcagcaa
     1261 catccatcag gctcgatgaa gaacttaagg accggctgaa aacgctggcc gatgaccggc
     1321 accgctcagc gcacgcgcta atgctggagg caatcaccga atacatcgac cgggaagaga
     1381 agcgcagcca gtacctgcgg gacggacagg ccgcatggca gcactatcag gaaacgggtc
     1441 tgcaccttac tgcggaagaa gcggaagcct ggataagtac ctggggaact gagaacgagc
     1501 aggatgctcc gccatgccac aggtaacgat ttccgcgctg gcacagcgcg atttacaacg
     1561 ccttcaggac tttctcaaaa ccaaaaatcg gctggcagcc agaaaaggag gtgaggtgat
     1621 cgtccgggct atccagcaac tgaaaacatt gccagacatt ggccgcccgg tgccgtttct
     1681 gccgctggaa tataaggaac tggtgatcgg gtttggcgac agtggctatg tgatgctcta
     1741 ccgccacgac cgggaaatgg accagattgt gatcgtcacc gtcaggcatc agaaagaatc
     1801 cgggtatccg ggggcagaca gcctctaata accgatgacg ggggcgcaat tcattgcgtg
     1861 gccgtcgccg gtttgacaag cgcagcgcgt cagaaaacca catcctgaac ggaaaaacgg
     1921 gtaaaattca gcgatacatt taccgtgaga aattacacac ctttcacacc cgataaaatc
     1981 aggggtatac agtggcgaaa acattgctca acctttcaca ggcagcacaa gccgcgggca
     2041 ttaccagaag aacgctatac aatcatgtaa aacagggaaa ggttaccgtg tcacgtgacg
     2101 ggaaaaacaa ccctgtcgtg gatgtgtcag aactgatccg tgtatacgga aatgtgaata
     2161 tccctgagaa acaaattccc ggcatttcac accgtgaaaa tacacaaaaa aatttcccac
     2221 aggaacagct tctggcaatg caaaaggaac tggccgatct ccggcaggct gtcaccctga
     2281 tgctggaaga taaaacatcc cgggaagaag aacgccgcca gcacgatgat gagcgccgga
     2341 aactccaggc cgaagtggac agattgacaa cagaactgac acagaagaaa aaacgcttct
     2401 ggtccggctg gtttagctga gaacttaccg aagggaatgg agaacatgac gaagcaggaa
     2461 aaaactgccc tcaatatggc taaattcatt aaggatcagt cgctgctgtt gctggaaaag
     2521 ctgaacgagc tggacatgga taccccggcc gacatgtgcg aacggcttca cgaggatgcg
     2581 gaaacactct ggcaggtgat gcgggataag ctcggggaag aataacagca ccggccatgc
     2641 cggtgctggc gttatttttt gccgaacggc caccaccggc gggcgggttt gtcctccggc
     2701 agttcaggca ccggcacgtt aaaatagcgg ctgttatatt cccgcatcag ctccgctttc
     2761 agcttctcgt tcaccgcgct ttccccgtgc cgctcgctga ccacctcaat catcgcgtta
     2821 atctgctccg gtgagcactt cccggcatca accacctgca caccgttctc gatggcgaac
     2881 cgctccacct ttcgatagag gtaatcacac agcttcattc ctgtgggttt gtcccgttcc
     2941 cattgcggat tttcaccggt gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg
     3001 tcatacatcc cgatggattt catcatcgcc agatgctggg cccggtcgac aaaacagaaa
     3061 acatacgccc cggtctgcca ggcgagcacc accatgtctt ccagatgctc gctggttggc
     3121 ataatgcagc gctcgccgtc tttgtcttcc acgtcctcaa taaataccat gtacagcgcc
     3181 ataatccctc actccgttgt cataaattta aagcacgcga acagcctctc ccggtccagc
     3241 tccaccgttt tgcgggcctc gccggtctgt ggattcacga tttcatccac cgccagaatc
     3301 gcccgctgta cccggacaag cgtcggccgg tcagggcaga tgtaccagat ttcatcgatg
     3361 ccgttcgccc ggcggttgaa cagatacccg gacaccactt cctgataccg cctgcggctc
     3421 ttgacggtgc gctctacctc aaatgccacc gttctgccgt ccagctccgc cagcgcgtcc
     3481 ggcactttca tccctttcag ggccatacgg tgcaggtacc gccagccggt ggccccccgg
     3541 ccctccatgg agaggcgcag ggactgcacg gccagccggt gcggcagctg cgccgccgac
     3601 acccttgacg gctgaaacgg aataacgtcc gtcagcggct cgtcctcgtc gaacgagaac
     3661 gccaccccgt gcggcgtcag cccccacacg ctgacctgac ggccgtcaac cgggtatttc
     3721 gcccggacca gcagcccttt cgccaccaga ctgtccagga agcggtaaac cggctgtacc
     3781 gccgcaatcc ccagccgtcg cgcaaccagc tccgccgtgg aacacagctc ttcccgcagc
     3841 cagcgcaggg tcagccgtgt tttctccgcg ttccgcgcct gccgcttcgc gtggctgacg
     3901 ttgtactgct ccagtgtcat cagtaaagcc ccgcaaaatc atcatccgct tttggtccgc
     3961 cgccgtcggc cggaggcagg tactccgcca gcgggtccgt tttcgccagc accgggaagg
     4021 ttttcagcga aatttcccgg cgctcaaccc gcagcgggct gctgaacgcc agccgcgcca
     4081 cgcccagccc ggtcatcacc gcgaacccgt caggcaggtg ttgcagggtg ttcacgtcaa
     4141 acagcggccg cgcctccttc tggatgaagc tgtcgccgct gatgtgctcc acgttccccg
     4201 cctcccggct ggcccggcgg cgctccacgt ccacccggat ctccccggtc tggcccgccg
     4261 cccactgcgc ggactcctgg ctggccgcgc gatagaacca gcggatcggc gtgttatcca
     4321 gtaccgtggt tttcacaaaa tcagcgggca gatcctgccc gcactgaccg aagtcgccca
     4381 gcgactggtg cgcgagcagc aggttgcagt cccggctgcg cagcgtcccc agcgcgttca
     4441 gcacgtactt cgacagcaga tatttaatct catcgagcat gatgctcgcg tgcggccacc
     4501 tccggaactc atcccgcgca atgatgattt gcgcgcagcg ggcaaacagc atcttctgca
     4561 cccggatcac cgcctcatcg tccatcgagc cgatgacgta caggcagccg ccgccgttga
     4621 ttatccccgc cacatcgata ccgctgtccg tctgcaggac gctcaggtcg gccacgttct
     4681 ccagcttggt gatcagcccc ttcacgtcct ttttcagggc ctccggcaac gcgtacgccg
     4741 ccgccagcac ctgctgaatg tttgccccct gcgggaactg ctccgcaatc agcttcgccg
     4801 ctttctgctc gctgatgcgg tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc
     4861 cggcaaccag cagattcttc agggcgtact ggtcgagatc gcggaacaga ttcagctgcg
     4921 ccggtttccc cggccgcaga tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt
     4981 gcggcgccca cgcgtcccct ttggggtcga agtacaccac agcgtccccg aaatcgcgta
     5041 cgcagcgaat gagcgcgttg gtggccatcg cgctcttgcc gctcccgggc aggccgagga
     5101 tctgaatatt ggtcttgcgc caggtatcac gagggaccag caccggctgg ccctgctcat
     5161 ccttacccag aaacaccgcc tgctgtttcc gggcctgctc cgtggaaaaa tcggccagcg
     5221 cgttaaccgg taccggctcc ccgaaatgca ggtggcggac gtctgtcatt tctccggtcc
     5281 gctggctgct gcgccggctc tcctgattca gtttcgcgtt caccgcctcg tcagcggccg
     5341 caaaatgacc atcaagcagc aggaacgcca gcagccgcgc cagaaggacg ccggcgacaa
     5401 gccctccggc acaccaggct atcccgaaca ggttagccgg ctgcagcagg taataatctg
     5461 cgttcgcata gaggtccggg cgcaggacgg tttctcccca gctctcgata aacacggcga
     5521 caaccagccc ccccagaagg gccagaccgg cagcgccggt cagccagtac gcgtagttcc
     5581 agacatgtcg ccgtgccctg accatccgcc cgggtgaatc cagcgaggcc agcaccgaaa
     5641 atggcacagt ccggttatta tcgcgcttat agcgatcggc atactcccgc cgcgccttat
     5701 ggctcagccc cacaaaacgc attcggccgg cccagagttc agccagaccg aaaaacaggc
     5761 cgataaccag aaaaaaaccg gccagactgt aatactcccg gtacagccag ctgacccacg
     5821 ggtaaatctg ccgggccagc tccgggatat gccactgcca ggcaagcgaa tccgtattaa
     5881 acatgcaatc tctctccttc agacgacggg tttaccggcg tgatgcgcag cccccgctgc
     5941 acaagccgcg gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt
     6001 cagctttcac cgcggaggcc agcctctttc cgccccatgc ggaaaaggcg cagccccgga
     6061 ggaacaacga gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt
     6121 ccggtgttcc ggcccccgtc agagttcgcc atagcgagcg gctgaccacc cgcaaaatca
     6181 ggagcagctt tgctgcttcg tcaggtgcgt ccagcaccgt ggatttttgt gggtggtcag
     6241 gcgtgagcct gacgggggcc gggtgaattt ctggcgtggc cggcgtccag ccggacagag
     6301 ccagaaagag gggaaaagac cgtgcgtcgg tggcgtccag ccagcgtaag caaggtcttt
     6361 tcccctgaag tcagccacgc cccagcgggg atgacttgca tttctccgca aggagaaaaa
     6421 agcaacagag ataggttaac gtaaaatgtt cagcgaaaaa ccgacatggt tcacctatcc
     6481 tgataattga tcgtcaggga atagaaagac ttaatcaggg gacaatatcc cacatcagcg
     6541 cgtccggtga ccgtcttgca ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct
     6601 acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt
     6661 ccgtggataa gcaggggact aacatgtgag gcaaaacggc agggccgcgc cggtggcgtt
     6721 tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt tcggtgcatc
     6781 tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt
     6841 aaagatcccc accgtttccg gtaggtcgct ccctcttgcg ctctcctgtt ccgaccctgc
     6901 cgtttaccgg atacctgtcc gccgttctcc cttgcgggaa gtgtggcgct ttctcatagc
     6961 tcacacactg gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag
     7021 aactccccgt tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc
     7081 cgggaaagca cgcaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga
     7141 tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga
     7201 aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca
     7261 actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa
     7321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgcg
     7381 atccccgtca gttcagagga ggacgatggt gccaccgttc cgccctgaac atcccggata
     7441 taatttgcct gacatgcggc cagaagaaag ccagcaacaa tcaatataaa aagaaaaata
     7501 gtctttacct ttttcatata ctttccctgt atttaaagac tccggcattc atccctgaac
     7561 accggaagtc agtatttcaa atcatccttg ataatttttt gttaccagtt accgtcccga
     7621 taatcaaaag ctatataaca attatccaca tcaggtttta tttgattttt aaaaaatggc
     7681 tgtagtaaag gaacccattc tttcttcaaa tcaaactcac cattattaat attatccttt
     7741 aaaggcattc ccaagctttc aatgacagaa ccatcgtcac caaagtcttt agaatactct
     7801 tcccctttaa actcttcagt tttcttatca aaccatgtca aacgtaattt aagccccata
     7861 tttacctctc aaagatattt tttaatgttc cgttttggat ctggcccttt aacctgctta
     7921 cccgttttgg gatcaaatgc gcctaggtgt tcgccatcac tggcacgata cccttcaagc
     7981 tcaccgtgct gggaatccca ttcataaatt ttacgttttt tatcgccata ccatcgggcc
     8041 cgcttaccac caccaccttg tttaggtgtt ttaggatcac ctcgctttaa ctctccaagt
     8101 ccctttatgt cttctgtttt aggtactgga tgataatcat ggccgtagtc tttagtcccc
     8161 ttgcgaggct tgttcttttc ctcatttaac ttattttctg catccttcgc ttttttctcc
     8221 ttttgtttcc ggctctccat cgccccggta agtgcagcct tatctctgga taaggattca
     8281 ttcattgcgt ttaaagatgc ctgagccgct tttaatttct cattctgatt ctgaatatcc
     8341 ttatcaagat agcgaacctg atcctgccac ccggagccat attcatgagg cgggttataa
     8401 gtcacaaaat catctttatt tgcctctgcc tcttttacct ttttatcggc ttcctgaaca
     8461 gcagcccttg caccggggag cgccttctct gtcgatgcaa tgcggctgtt taaggtcgca
     8521 atatttttat cttcggcatc cagctcagct ttcgctttat catagtctct tttcagcccc
     8581 tcttccggat gagcggcgtc ccatgcctgc tggcgacgct tttcttcctc ctgacgctgt
     8641 tttacctgtg ccggggtaag aacatctgtc accgacacat aaaccggctt ctgtccggtc
     8701 tctttcggga aacgaataac ggcctcacgg gagttaccac cggctgtaaa accagccgga
     8761 cgtgaatcac ctttttcagc aacaatgcct tttggcgggg ctttcgctgt cggaacacct
     8821 ttcggtacgc tcacctgcag agacgggaga cccggaatcg acacactgaa tacccccgga
     8881 cgttttgtcg gtttcgcatc caccacaggg acactcattg gccgacctgc gacaaccgca
     8941 atatgctgcc gctcatcctt caccacatcc acaacccgtt gtctgacacg aaccgtcgcc
     9001 tggtccagtg gtaaagtact tgccggagtc tccgtcaccg catcggccgg taatgacgtc
     9061 ataatttttg acatcatttt cggatcatct tttgcaatct cagaaggcag cacaccgtag
     9121 atcgcaatcc cccacagacc aaacttaaac ggccccttca gggcagccag cacatcagca
     9181 acggcggccg acaacgcatc gccggaaact gataaagcca gtccttcagc ccccggagta
     9241 gccagggcag gcagaccgaa ggccatcgca gtggccgaag actgtccgcc accagagcta
     9301 ccgctatgat tgccgctgtt accaccatta ccaccgggtt tactcccgcc gttaccaaac
     9361 tcccccggat tatagttatg aatatctcca ttcggcgtat gcgtcgtacc ccagcccggc
     9421 ccgctgtttg gatccgtacc accacctgat gaagggccac ctttaccaga cgtaccgttc
     9481 acattcccac tggcctgacc accattatgt cccagacctg aattacccgg acctcgaccg
     9541 tctccaccac tcataccgtt ttcctctttg acatttaaaa caataaatta aagccaacat
     9601 actgtacata tacacagtat aattattacc ttactcctcc cgttttcagg tgtcaatata
     9661 tgagattagt tctcatcttc aggcacaaaa aagcgcccgg agccacctgt aagcagatcg
     9721 cgtcttttcc cgaaaatttc tcatggcaac aaattttatt atttaagaca ttcatcacac
     9781 ttacatttca gatggtatag acacatctga aagctaactg gcatgaggag aggacctgcg
     9841 gttaaactgt tcgggaaacc tgagacgccc ggaggccact atgacgcacc tgaaactgac
     9901 tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct caggcactgg ccatgggcct
     9961 gaaagggaac aaaacgctgc caaaggcggt aaaacaacag gtgcgggaaa gggtgcttcg
    10021 tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa
//
