LOCUS       NZ_MK024374             2381 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain 9696 plasmid p9696_4, complete
            sequence.
ACCESSION   NZ_MK024374
VERSION     NZ_MK024374.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Sadowy,E., Gawryszewska,I., Kosecka-Strojek,M., Miedzobrodzki,J. and
            Hryniewicz,W.
  TITLE     An outbreak of linezolid-and meticilin-resistant Staphylococcus
            epidermidis in a neonatal ward
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Sadowy,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2018) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw, mazowieckie 00-725, Poland
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: CLC v. 9
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038098375.1-RS_2024_04_17
            Annotation Date              :: 04/17/2024 12:45:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MK024374.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="9696"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p9696_4"
                     /geo_loc_name="Poland"
                     /collection_date="2015"
     gene            1..999
                     /locus_tag="AAFM56_RS00005"
     CDS             1..999
                     /locus_tag="AAFM56_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_078153474.1"
                     /translation="MQHNTTRYIDENQDNETLKDMTKSGTQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLYKTWFCKSKLCPVCNWRRSMKNSYQAQKVI
                     EAVVKEKPKARWLFLTLSTKNAIDGETLEQSLKHLTKAFDRLSRYKKVKQNLVGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENSYFKNKANYITQEEWINLWQKALQVDYKPVANIKAI
                     KPNKKGDKDIQAAIKETSKYSVKSSDYLTGNHEKDAEIVQDLEQGLYRKRMLSYGGLLK
                     QKHKLLNLDDAEEGNLIQTSDEEKTTEEEQKAHSITAIWNFEKQNYFLKNL"
                     /name="protein rep"
     gene            1344..1541
                     /locus_tag="AAFM56_RS00010"
     CDS             1344..1541
                     /locus_tag="AAFM56_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020367458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_241526587.1"
                     /translation="MKKDMPEKTKLGNSLSFNYFLLLVGVLTFLGYYFLGDSNIMISWL
                     LAMCPITVGIANIGRIKNEK"
                     /name="hypothetical protein"
     gene            1744..2163
                     /locus_tag="AAFM56_RS00015"
     CDS             1744..2163
                     /locus_tag="AAFM56_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosfomycin resistance bacillithiol transferase
                     FosB"
                     /protein_id="WP_032488468.1"
                     /translation="MIKGINHITYSVSNIAKSIEFYRDILGADILVEGETSAYFNLGGI
                     WLALNEEKNIPRSEIKYSYTHIAFTISDNDFEDWYIWLKENEVNILEGRDRDIRDKKSI
                     YFTDLDGHKLELHTGSLEDRLSYYKEAKPHMNFYI"
                     /name="fosfomycin resistance bacillithiol transferase FosB"
     ARG             1745..2160
                     /name="fosB"
                     /gene="fosB"
                     /gene_name="fosfomycin resistance bacillithiol transferase
                     FosB"
                     /tool="amrfinderplus"
                     /drug_class="FOSFOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1745..2163
                     /name="FosB4"
                     /gene="FosB4"
                     /gene_name="fosfomycin thiol transferase"
                     /tool="rgi"
                     /drug_class="phosphonic acid antibiotic"
                     /identity="99.28"
                     /coverage="100.0"
     Typing          2..999
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.394"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaacata atactacaag atatatagac gaaaatcaag ataatgaaac attgaaagat
       61 atgactaaaa gtgggacaca acgcccatgg agagaaaaga aaatcgacaa tgtaagttat
      121 gcagatatac tagaaatttt aaaaattaaa aaggctttta acgtgaagca atgtggtaac
      181 gtcttagaat ttaagccgac tgatgaaggg tatttaaaac tttataaaac atggttttgt
      241 aagtctaaat tatgtccggt ttgtaattgg aggcgttcaa tgaaaaatag ttatcaagct
      301 caaaaagtca ttgaagcagt ggttaaagaa aagccaaaag cacgttggtt attcttaaca
      361 ctttcaacga aaaatgcgat agatggtgag actttagaac aaagtttaaa gcatttaaca
      421 aaagcttttg atagattgag tagatataaa aaagttaagc aaaatcttgt tggattttta
      481 cgttcaacag aagtgaccgt taataaaaat gatggcagtt ataatcaaca tatgcatgtt
      541 ttattgtgtg ttgaaaatag ttattttaag aataaagcta attatataac gcaagaagag
      601 tggatcaatt tatggcaaaa agctcttcaa gttgattaca aaccagtcgc aaatatcaaa
      661 gcaattaagc cgaataaaaa aggcgataaa gatatacaag ctgcaattaa agaaacatca
      721 aaatactcgg ttaagtcgtc cgattattta acgggaaacc atgaaaaaga cgcagaaatt
      781 gttcaagatt tggaacaagg attatacaga aaacgcatgt taagttacgg tggtttgctt
      841 aaacaaaaac ataaactttt aaatttagac gatgccgaag aaggtaattt aattcaaacg
      901 agcgatgaag aaaaaacaac tgaagaagaa caaaaagccc attcaattac ggctatttgg
      961 aatttcgaaa aacaaaatta tttcttaaaa aatttgtaac gttagcttat caaagctagc
     1021 gtttttttaa gcttttttgc ccctgcagga actatagcgt tcaaccggca tggttgccca
     1081 gtttttatgc caatttttaa atttgcatgt aactgggcag tgtctaaaaa attcaacact
     1141 ggttttgctc aaatttttgt ttgtgaaaag caattatttt tatttggctc tcatttgcgt
     1201 tttaagagcc aatataagtt tttaggcaat aaactatata aataacccct aattctttaa
     1261 aatgccccct taaaattaaa aataaaggca ttataatttt tattaagtct ttaaatattt
     1321 tgttagcaca aagaataagg agcgtgaaaa aagatatgcc tgaaaaaact aaattaggaa
     1381 attcattgtc ttttaattat tttttattac ttgtaggtgt attaactttt cttggttatt
     1441 attttttagg agattctaat atcatgatta gttggttact ggcaatgtgt ccaataactg
     1501 ttggaattgc aaatataggt agaataaaaa atgaaaagta gattatgcat ttatgccgag
     1561 aaaatttatt gatgttgaga agaaccctta actaaacttg gagacgaatg tcggtatagc
     1621 gtgagctatt aaaccgacga ttcgacaagt ttagggattg ttaagggttc cgaggctcaa
     1681 cgtcaataaa gcaattggaa taaagcaact atgttaaaat tttaaaaaaa ggggagggag
     1741 aatatgatta aaggaataaa tcatattact tattcggttt ctaatatagc taaatcaatt
     1801 gaattttaca gagatatttt aggggctgac attttagttg aaggtgagac ctcggcctat
     1861 tttaatttag gtggtatatg gttggctttg aacgaagaaa aaaatattcc tagaagcgaa
     1921 attaaatatt cgtatactca tatagcattt acaatttcag ataatgattt tgaagattgg
     1981 tatatctggt tgaaagaaaa tgaagtaaat attcttgaag gtagagatag agatattaga
     2041 gataaaaaat caatatattt cactgattta gatggtcata aattagaatt gcatacagga
     2101 agtttagaag atagattgag ttattataaa gaggctaaac ctcatatgaa tttttatatt
     2161 taaaacaaag ttatgtgttt tgttttgaaa cgagtgaaaa cgagtttctt cttgtcttga
     2221 tactatatag aaataactcg atttttaaat tatcgcttta tcccttgtca atagagggtt
     2281 aaggcgtttt tttgttgttg aaagaaattt ttgacataaa aaaaccccag tagtatgatt
     2341 aaagcgtcta ctcaataaac atctactgga ggtcttttta t
//
