LOCUS       NZ_MH785254            20653 bp    DNA     circular CON 21-JUL-2023
DEFINITION  Staphylococcus aureus strain ch24 plasmid pMW2, complete sequence.
ACCESSION   NZ_MH785254
VERSION     NZ_MH785254.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05853514
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20653)
  AUTHORS   Bukowski,M., Piwowarczyk,R., Madry,A., Zagorski-Przybylo,R.,
            Hydzik,M. and Wladyka,B.
  TITLE     Prevalence of Antibiotic and Heavy Metal Resistance Determinants and
            Virulence-Related Genetic Elements in Plasmids of Staphylococcus
            aureus
  JOURNAL   Front Microbiol 10, 805 (2019)
  REMARK    DOI: 10.3389/fmicb.2019.00805
REFERENCE   2  (bases 1 to 20653)
  AUTHORS   Bukowski,M., Hydzik,M., Sabat,A.J. and Wladyka,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) Faculty of Biochemistry, Biophysics and
            Biotechnology, Department of Analytical Biochemistry, Jagiellonian
            University, Gronostajowa 7, Krakow, malopolskie 30-387, Poland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 01:09:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 25
            CDSs (total)                      :: 25
            Genes (coding)                    :: 21
            CDSs (with protein)               :: 21
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 3 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MH785254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ch24"
                     /isolation_source="Broiler chicken"
                     /host="Gallus gallus"
                     /db_xref="taxon:1280"
                     /plasmid="pMW2"
                     /geo_loc_name="Poland: Wroclaw"
                     /collection_date="2008"
     gene            228..904
                     /locus_tag="BJL71_RS00005"
                     /pseudo=""
     CDS             228..904
                     /locus_tag="BJL71_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            <915..1197
                     /locus_tag="BJL71_RS00135"
                     /pseudo=""
     CDS             <915..1197
                     /locus_tag="BJL71_RS00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2406..3023
                     /gene="cadD"
                     /locus_tag="BJL71_RS00015"
                     /old_locus_tag="BJL71_h00015"
     ARG             2407..3020
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            3042..3389
                     /locus_tag="BJL71_RS00020"
                     /old_locus_tag="BJL71_h00020"
     CDS             3042..3389
                     /locus_tag="BJL71_RS00020"
                     /old_locus_tag="BJL71_h00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3558..3650
                     /locus_tag="BJL71_RS00025"
     CDS             3558..3650
                     /locus_tag="BJL71_RS00025"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3862..4836
                     /locus_tag="BJL71_RS00030"
                     /old_locus_tag="BJL71_h00140"
     CDS             3862..4836
                     /locus_tag="BJL71_RS00030"
                     /old_locus_tag="BJL71_h00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072390.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(4956..5771)
                     /locus_tag="BJL71_RS00035"
                     /old_locus_tag="BJL71_h00030"
     CDS             complement(4956..5771)
                     /locus_tag="BJL71_RS00035"
                     /old_locus_tag="BJL71_h00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(5764..7207)
                     /locus_tag="BJL71_RS00040"
                     /pseudo=""
     CDS             complement(5764..7207)
                     /locus_tag="BJL71_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(7179..7772)
                     /locus_tag="BJL71_RS00045"
                     /old_locus_tag="BJL71_h00045"
     CDS             complement(7179..7772)
                     /locus_tag="BJL71_RS00045"
                     /old_locus_tag="BJL71_h00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(8036..8416)
                     /gene="blaI"
                     /locus_tag="BJL71_RS00050"
                     /old_locus_tag="BJL71_h00050"
     ARG             complement(8040..8416)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(8406..10163)
                     /gene="blaR1"
                     /locus_tag="BJL71_RS00055"
                     /old_locus_tag="BJL71_h00055"
     ARG             complement(8410..10163)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            10270..11115
                     /gene="blaZ"
                     /locus_tag="BJL71_RS00060"
                     /old_locus_tag="BJL71_h00060"
     ARG             10271..11112
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11484..12017
                     /locus_tag="BJL71_RS00065"
                     /old_locus_tag="BJL71_h00065"
     CDS             11484..12017
                     /locus_tag="BJL71_RS00065"
                     /old_locus_tag="BJL71_h00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001823239.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNESK
                     RRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEVNIT
                     RQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            12431..12724
                     /locus_tag="BJL71_RS00070"
                     /old_locus_tag="BJL71_h00075"
     CDS             12431..12724
                     /locus_tag="BJL71_RS00070"
                     /old_locus_tag="BJL71_h00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            12799..14733
                     /locus_tag="BJL71_RS00075"
                     /old_locus_tag="BJL71_h00080"
     CDS             12799..14733
                     /locus_tag="BJL71_RS00075"
                     /old_locus_tag="BJL71_h00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            14737..15048
                     /locus_tag="BJL71_RS00080"
                     /old_locus_tag="BJL71_h00085"
     CDS             14737..15048
                     /locus_tag="BJL71_RS00080"
                     /old_locus_tag="BJL71_h00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            15060..15686
                     /locus_tag="BJL71_RS00085"
                     /old_locus_tag="BJL71_h00090"
     CDS             15060..15686
                     /locus_tag="BJL71_RS00085"
                     /old_locus_tag="BJL71_h00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            15924..16553
                     /gene="mobV"
                     /locus_tag="BJL71_RS00140"
                     /pseudo=""
     CDS             15924..16553
                     /gene="mobV"
                     /locus_tag="BJL71_RS00140"
                     /inference="COORDINATES: protein motif:HMM:NF013258.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            16647..17192
                     /locus_tag="BJL71_RS00100"
                     /old_locus_tag="BJL71_h00105"
     CDS             16647..17192
                     /locus_tag="BJL71_RS00100"
                     /old_locus_tag="BJL71_h00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000273005.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            complement(17234..17518)
                     /locus_tag="BJL71_RS00105"
     CDS             complement(17234..17518)
                     /locus_tag="BJL71_RS00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254231383.1"
                     /translation="MKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQKHSFYVA
                     KTLLLCSKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGATYFSS"
                     /name="hypothetical protein"
     gene            17576..18436
                     /locus_tag="BJL71_RS00110"
                     /old_locus_tag="BJL71_h00110"
     CDS             17576..18436
                     /locus_tag="BJL71_RS00110"
                     /old_locus_tag="BJL71_h00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000145511.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            18523..18864
                     /locus_tag="BJL71_RS00115"
                     /old_locus_tag="BJL71_h00115"
     CDS             18523..18864
                     /locus_tag="BJL71_RS00115"
                     /old_locus_tag="BJL71_h00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(19103..19174)
                     /locus_tag="BJL71_RS00120"
     CDS             complement(19103..19174)
                     /locus_tag="BJL71_RS00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            19323..19508
                     /locus_tag="BJL71_RS00125"
                     /old_locus_tag="BJL71_h00125"
     CDS             19323..19508
                     /locus_tag="BJL71_RS00125"
                     /old_locus_tag="BJL71_h00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(19727..20470)
                     /locus_tag="BJL71_RS00130"
                     /old_locus_tag="BJL71_h00130"
     CDS             complement(19727..20470)
                     /locus_tag="BJL71_RS00130"
                     /old_locus_tag="BJL71_h00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843757.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEAKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             10271..11115
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             7431..17724
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1255..1402
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          17577..18436
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19728..20470)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15925..16199
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 attcaaagtg ctataataac cttaatataa caaaaacccc aactactggt aatagtcggg
       61 gtttggtaat aaaacgttta gaaactgttt atttatataa ttttcgatac ttagattata
      121 taatgaataa tatcttagtg caagcaccta gattattaaa taaacacaaa aaatgtaagc
      181 gttttattac ctcttaccaa actaaaattc ggaaagaggt ttttattatg tcaattaaac
      241 tttacaacat aaatgaggaa tacagagaaa agttttatca aataccgaaa gtatttttta
      301 caaatcctaa atatatcaag ttatccaatg atgcaaaaat ggcatggggt attcttagag
      361 atcgtttaga tttatcaatt aaaaatggtt gggttgattc taaaacaggt aatatttatt
      421 tttattacaa aaatgaaaac ccacaaaata tattaaattg taaaaaagat aaagtaatta
      481 aaataaagaa agaacttaat tgaagccgaa ttactactac aagaaagaca aggtttaaat
      541 aagacaaata aactttattt agttaaacca gaagtaacag aagatgatat ttataaaatt
      601 caaaagcaag aaaacactgt tgagagcagc aatggcaagg aagtcggaaa aaccgacttc
      661 aagaagtcga taaaacagaa tctaatgata ctgaacttag ctatactgaa tctaacaata
      721 ctgaaataaa tgatatgaat gacttgaatg atatagagtc cgaaaatgaa attagtaatc
      781 attcaagtca atgcaaggtt atttaatgca aacaatttta actgaatttg aagaaacaca
      841 tgcactttat atgcgccgta aaaacatgaa acaatataat atctttaatc aatcattcaa
      901 atgattttat aacaatcaaa gatacatcaa aatatttcgt taaatcatct gactttttaa
      961 ctgatagcga tgaaaaaatc aagaaattgt cagtgatcta gaaaaagatt tgtatcgaaa
     1021 acgtatgtta agttatggtg gattgcttaa ataaaaacat aaaattttaa acttagacga
     1081 tgctgaagat ggcaatttaa ttaatacaag tgatgaagat aaaatagcaa atgaagaaga
     1141 aaaggcacat tcgattacgg caattttgaa aagcaaaatt attatttaag gcattagtga
     1201 taactgatgt ctttttttat tacttttttg taaaaaaagt gctgtcttat ttttgtgaca
     1261 aatgctgtat gtagtgtcac aaaagtgtga cacttgagct ttttatgacc ccacacaaaa
     1321 acattttcga agccttgaaa tatctggctt tgccagatcc cccattcatt gatggggaca
     1381 aatttccctt atgctcttac ggagatttta gagttaaatt aaaatttcta agtttttgat
     1441 aagaaaagcc ctgcaggaat ttagaagaac gtggggaagt tttaaagaat tttatgctaa
     1501 tttttaattt gcatgtaaat gggcagtgtc taaaaaatag ttattgtttt tgctcaaatt
     1561 tttgtttttg aaaatagcat atatttattt ggctcatatt tgcgttttaa aagcgtatat
     1621 aagttttaga taaaactata tgaatcaccc ctaaatcttt aaaatgccct cttaaaattc
     1681 aaaataaggg cataaaaaat aaaagccccc ccagataaag tgaggtagct ttttctttcg
     1741 acgccgtatc gtcgatgtaa ttcaaaaaag ttatgggcta taaatctaca tcactttttc
     1801 aagaatgtag tagcaatatt caacgcaaat taatttttat aactgctcgg aatatctcaa
     1861 gccgtttctt taattttgaa ataaaaaaat cgacgaaggt cgattacgtt tttgcacatg
     1921 tcgatttatc cgacgtataa gtgcgccctt acgggattta actagattat aacgacgaat
     1981 tttagacctg taaagcaata acaattaggt ctaccaggta gataaagtgt acaaagtaga
     2041 accgaaaaag ttatttattt tgaagatttt tcgtagatat aatttgctaa taaacttcct
     2101 aaaactgaat aaagaaaaat agttaaactc atgtaatcac tccataattc tgaagtgtct
     2161 ccatgtctac gcatagacac gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac
     2221 ccttaactaa acttgcagac gaatgtcggc atagcgtgag ctattaagcc gaccattcga
     2281 caagttttgg gattgttaag ggttccgagg ctcaacgtca ataaagcaat tggaataaag
     2341 cacctatgat tatttaaaaa tataagttat aatacattca aaaatatttt tgaatgaggt
     2401 gtattatgat tcaaacggtt gttgctgctg ctgttcttta tattgctaca gcagttgatt
     2461 tattagtaat tttattaata ttttttgcta gagcaaagac tagaaaagaa tatagaaata
     2521 tttatgttgg tcaatattta gggtctatta ttttaatatt agttagtttg tttttagctt
     2581 ttgtattaaa ttatgttcca gaaaagtgga tattaggttt attgggttta ataccaattt
     2641 atcttggaat taaagtggct atttatgatg attgtgaagg agaaaagaga gctaaaaaag
     2701 aattgaatga aaaaggattg tctaaattag ttggtacggt tgcaattgtt acgatagcaa
     2761 gttgtggcgc cgataatatt ggtttatttg ttccttattt tgtgacatta agtgttacta
     2821 atttattact tactctgttt gtctttttaa ttttaatttt cttcttggtg tttactgcac
     2881 aaaaattagc taatatccca ggaattggag aaattgttga gaaatttagt cgttggatta
     2941 tggctatcat ttatatagct ttaggtttat ttattattat tgaaaatgac actattcaaa
     3001 caattttagg atttatattt taatttaggg tgtgatttta tatgagttat gaaaatactt
     3061 gtgatgtgat ctgtgtacat gaggataaag ttaacaatgc tttaagtttt ttagaagatg
     3121 ataaatctaa gaaattactt aacattttag aaaaaatttg tgatgagaag aaattgaaaa
     3181 tcatattatc tttgattaaa gaagatgagt tgtgtgtttg tgatatttct ttgatattga
     3241 aaatgagtgt tgcttcaact tcacatcatt taaggctttt atataaaaat gaggtacttg
     3301 atttttataa agatggaaag atggcatatt attttattaa agacgatgaa ataagagagt
     3361 ttttctctaa aaatcaggag ggtttttgaa acgagtgaaa cgagtttctt cttgtcttga
     3421 tactataaaa aacaaagtta tataacataa gacgacttat cggaagttat ctatcatcgt
     3481 atattcggag atgatataat atacaggtcg cctatctctc aggcgtcaat tcagacgcag
     3541 agaggaggtg cattactttg ctaatcattt tcgttcacat catagcacca gtcatcagtg
     3601 gctgtgctgt tgcgtattat acttattggc ttagtaaacg caacaaataa ttaataggcg
     3661 actttataag ccagcatcga aaaaatcccc acactattgc agtagtgtgg ggattggtgc
     3721 attaattgtt aatcattttc gttatctaga ttataacact ggttagtaga ggaatgcaaa
     3781 aagataatat tttaagtagt ctttttggat attatcatat gatatattta tgtaaattga
     3841 ttttttatta aaggagcata tatgagtaat actaaaatta aatatcaaat ttttatttca
     3901 tcaacttatt tagatttaat agaagagcgt caagcagtag tagaagcagt tttaaacagt
     3961 aataatattc cagcaggtat ggaacttttt aactcaagta gtaaaagcca atgggaaata
     4021 ataacacaat ggatcaatga atcagatgca gtgatattat tgttaggttc tagttatgga
     4081 acaattgata gtgaagaaaa tattagctat acagaaatgg agtacaattt tgctaaatct
     4141 ttaggtaaac ctatatatgt ttttgagatg aataaagcat ctaccgaatc cgcggaaaag
     4201 tctaaaaaat tagatgagtt taagacaaaa gttaaagaaa atagactaat tagtccaaat
     4261 attaatgata tttcggaatt aaaaagtaaa gtatctcaga ctttaatgca aagtgtacct
     4321 agtgaaatta aagaaaatag tggatggata agagtacagg attgtggtaa aaatgtaaaa
     4381 aaattaaaaa aattggatgc tatacaaaga gatttagata catatgattt tttgaaagaa
     4441 gaactttata aaatattcat gtatgtaaaa actttttatt tccagcacac accatttgat
     4501 gatagttata ttgaagatat agaaaactac ctgctaaatt atgaaaataa tccacataaa
     4561 tcattcatga aaataaaagt ttatgattat aaaaactttt taaaacattt aaaaaacata
     4621 ttgattttaa ttaaacaagg aaaaaagcca ttagctcaag ccggtcattt taaattaagc
     4681 ttttacgaag aggatttaaa attgttacaa aaaaattata aggctacgct agtcgatatt
     4741 aaaggattga ataaacattt ttataaggaa tgtgataggt gcttaaaagc atataatatt
     4801 tttattgagg gtataaataa acatttatat gaatgatata agtacaccct aatcatacat
     4861 atataattgt tgtcacttta ctcgatatat gagaatgact ttacctaata aatgagaaaa
     4921 gcaacgcact atattaaaat gcgttgcttt ttctattatc gaatacctat aactaaacta
     4981 tctctagctg tttctacaac ttctgtactt attttatcta aaccatttat atccataatt
     5041 ctgtctatct gcgcaaataa acgatgaatc agcctaaaat tcccttttgt aattttaata
     5101 atcgtcgtta ttgcctcata atcagtaaag tcttcgagtt ttaaatcaaa tcctaagtct
     5161 tgccacttat attctaatat atgatgcgtc tcatctttac ttagattatc aaattcatgc
     5221 gcaaaaccta tacgcgaata tagttgagga taacgagata gttttttctc tatacccggc
     5281 ataccaataa atatcattgc taaattatgt tcatcataaa tagctcttaa ttgctctaaa
     5341 tgttgcactt taaggcgatc aatctcatct actataatta aatcaatata tttaaacata
     5401 tcactataat gtttactatg ttcatgtcca tacttattta caacatgtaa tttttgaccc
     5461 aaatcaatga ttgcagtaat actatataaa tcattcctta atttagtttg tttttctgct
     5521 ggtgcattat aaaatatttt atccaccgat agtatgtcat cggtcgtttt actagctaaa
     5581 tcttcccaac ctctatggtt aacttgtttt tcaatagtat cccaatttgt gtaataccta
     5641 gacgataaag tttttccgac accaggttga ccataacata taccaatata ctgatattta
     5701 atacaagcat cacaaaattc agcaaagcgt ttatactctt tcgtttcaat gaaattttga
     5761 tttttagtca ttacgatatc tctttaattt tttgacgttt ttagtcgttt cttgaggtga
     5821 ataaccacaa tttttttctt ccttaattaa ttccgtggta cttggactag caatattttg
     5881 ttttaagtgt tttcttctct gactacgagc gtgctgtatg tcttttatat ctatggagta
     5941 atcagctatt tctggacata tagcagtaca aagaaattca tctctataaa acacacgtat
     6001 ttcagccata tcatttgggt tataacgaat cagtacatat tcacctacat aagcttctag
     6061 attagtatta ctgtatcgaa aaccttgaaa atgaatgcca tctgaatgaa cttttctaga
     6121 tttaggtatt tccaacaata ataaatcaag ttgttctaag ctacttggca tattagggaa
     6181 aaagtgatta ctattccaat gactgatagg cgtggtatga atagtactat gttctttttg
     6241 attataatct tcaattaaaa agtaacgtaa tttttcttca aaattgtgaa aatttattag
     6301 attactagga gtatcattat tgtttatata tccaggcagt tgttctaaga aagtttgatt
     6361 aactgtctga aagaatcgtt ctatttttcc acgaccacga ggaacaccaa ctttcgaaaa
     6421 catcaagtta attttcaaat caatagcgac ttgttccata tgatgtgaag taaagtcact
     6481 tccatgatct gtgtagaatt tttcgggtat accacacact ggccaattgg tatcattttt
     6541 attccatatt gcttggtgta gtgttaatgc tgtgttttga gcattggggg gcatcaaaac
     6601 ttaagaaata gcctgcaatt gcacgactat agtcatccat aataatagtt agccatggtc
     6661 tattgatatt acctttttgg tctaatataa agatatctag caaagtatgg tctgcttgcc
     6721 atatttcatt gggtctcgag gattctctaa tttgtattaa atcgtactta ttttgatagt
     6781 atttttcacc ctgatgagag aaatcaataa cagacttagg catcgcttta ataacactat
     6841 aaatttgttt ataacttggt atatcaaaat tattttcttt acagtaatta gtgattttcc
     6901 tatgaattgt ggctatacta tttcttttat tcataagcgc aaggcgttca atttcatcaa
     6961 ttatctttgg ttctatttta cgtgttcctt tatcagctct tgtcgcatag attagtcctt
     7021 taagtccttg ttctttatat gacttattcc acctataaag ggtgcttaac gcaatccctt
     7081 tacttttaga aatacttgat aaagattgtt cgcctaggat aaaaggtttt ataatattgt
     7141 acttttgagt agctttttta cgtttggctt cagaaaaatt agttagatac ttctccttgt
     7201 ttttcatctt ccttgtcctc caatttattg aggtaacgat aaattgttgt acgtgaaaca
     7261 tgccactgtt ctgcaattgt cttaattggc gtgccattat cataaagggt cttaagtaag
     7321 tttaaatctt tttgatttaa tttttctggt ctgcctccat atcggcctct agcacgtgct
     7381 gcaattcggc cagctgaaga acgctctaaa attaaattac gttcaaattc tgcaaatgct
     7441 gcgaataaat gaaataataa ttgtccagtt gaacttgatt tatccattgt tatattttct
     7501 tctaaactat ggaaacttac acctcgttcg ttaagctcat taactaatgt gattaaatcc
     7561 tccatattac gtcccaatct atctaatctc caaacaacaa tcgtatctcc tgacctcgca
     7621 aattcaattg ctttatctaa accaggtctt ttactttttg aaccactcat atggtcatta
     7681 aatatctttt cacaaccata tgtatttaat cgatcctcct gtaaatttaa attttgtaat
     7741 ccagttgaaa ctcttgcata acctattttc aaaatacaaa cctcctatat acatcttcag
     7801 tgtaccataa cttaattaca aggtctttta tgaacataga ttttttgttg ggtttttgaa
     7861 cacataatcc tatgtaattc ttacttattt agacataaaa gaagagtgta cagaaaatgg
     7921 ttattttctg tacactctca tctttaataa tatattttaa atgtcattaa caaaactaat
     7981 ttaataagag tcaagcatag tttacatatg tattatgttt gaaacatacc tctaattact
     8041 ttttactaat atcatttaaa atatctcgca attcttcaat ttctttgtta tttaattctt
     8101 catttttcgc aaaattcaac actaaacttt tcatatcccc tccatacagt ttattaagaa
     8161 aggttttagc agttttcatt ttaatatcgt cttctttaat atttgatgag taaaaataaa
     8221 tattttctga tttgtatcgt tttataatct cttttttata tagtcttgtg attaatgttc
     8281 taatcgtttt atcactaact tctttatatt tttgaatttc aactacaatt tcattagctg
     8341 atactgattt tttatcccat attatattca taacatccca ttcagccata gatatttcaa
     8401 cttgcttatt ggccatttaa aacacccatt ccttttaata ttttttcact tattaattca
     8461 gcatttttcc cagacgggtt tccatctgat aaatgtgtag caaaataata cttatcatga
     8521 tttgtaatta cgtaacctac aaaccaccca ttattatact tcccgtttac tatacctgta
     8581 cctgttttcc catacagttc atacttttca tttttcttaa tcaataatga agaagataat
     8641 tgattctttg cttttttact aaaatggtta ttttgttcca tcatattttt aaaaactatt
     8701 acttgttcaa gattagatat tttcaaacta tcttccatcc aatagctttt ataacttccc
     8761 aaattttcat taccataatt taattgcttg agttgagccg cagtatagtt ctttggtatt
     8821 tgattactaa tacgttcgaa gtaccaatta actgaatttt gcattgctgt atttaaatct
     8881 tgttccttat tccaagactc gaaaagataa tgtttatgat tccatgacat acgtgaattt
     8941 ttatcactta taatatgacg gtcaagtcca aacagtgcta aataaatttt gtaagttgaa
     9001 tcaggagaat atctttttct actttctttt tcattataaa tatagtattt gtctttcttc
     9061 atgctgtaca taacgaaaga tccactattt gaaccaaaat ttttactttc atctaaaatt
     9121 tggtaatcgc tttgtaaggg ctttttataa ttataatcag ttaaggattg ccccattaaa
     9181 aactggcttt gaattatcat gataaaaaaa gtaaaaatac aaataaaaat tagaattaat
     9241 tttgactgct ttttcagatc ggcttctttt atattaatta atctattttt gagtaatgat
     9301 tttttaccat taaatgaatg gcttaatata tttttgttga aaaatgaggt ttttaatacg
     9361 gagtccataa tagattccgc ataagtttta aattcatttt tgttaatatt attaaggaca
     9421 aatctatcgg cttctacttc attgtcatga attatctttc tttttacaat atgtactaat
     9481 ggattgtaac tcataataat agagaagata ttaaagataa ttaaatgtaa agtatctcta
     9541 tttttagcat gagcatattc atgcagtatt atatatttta gtcttttatc aattacactt
     9601 ttaaaatatg aactaggaat caaaataata tatttcccat accaaaaagt tattggagat
     9661 tgaatagcct ctgcttttcg aatcacaata ttttttttat attgatggtt gaaaagtatc
     9721 gtatctattt tatttttttc attttcgttt agataaagtg actgtttctt taaatattta
     9781 agatataata aggctttcaa aaatttaaaa cttaaaataa taactaaaac tatccaaata
     9841 actgtgcaga tattatcaat tgaatcccaa ttaaacttat ggatatctgt tgcgaactct
     9901 tgaacaggtt tggtggtaat tatgttatgg ttcaagtcgt gtgacttact ttcaactgtg
     9961 ggcgcttgat tattcacatt attaaatttt ataaaagaga atttaattgg gataaaagga
    10021 attaatccag caagcacagt tagataccaa actttataat ttaaactata attaaaatag
    10081 cgttttaata tgtacctaaa aaatactaac aatagaaata taaaacaaaa gcttactaca
    10141 ctcattatta gtaatttagc cattttgaca ccttctttca aatatttata ataaacaatt
    10201 gacatcaata ttacaattgt aatattattg ttttgtaaaa attacaactg taatatcgga
    10261 gggtttattt tgaaaaagtt aatattttta attgtaatgg ctttagtttt aagtgcatgt
    10321 aattcaaaca gttcacatgc caaagagtta aatgatttag aaaaaaaata taatgctcat
    10381 attggtgttt atgctttaga tactaaaagt ggtaaggaag taaaatttaa ttcagataag
    10441 agatttgcct atgcttcgac ttcaaaagcg ataaatagtg ctattttgtt agaacaagta
    10501 ccttataata agttaaataa aaaagtacat attaacaaag atgatatagt tgcttattct
    10561 cctattttag aaaaatatgt aggaaaagac atcactttaa aagaacttat tgaggcttca
    10621 atgacatata gtgataatac agcaaacaat aaaattataa aagaaatcgg tggaatcaaa
    10681 aaagttaaac aacgtctaaa agaactagga gataaagtaa caaatccagt tagatatgag
    10741 atagaattaa attactattc gccaaagagc aaaaaagata cttcaacgcc tgctgctttc
    10801 ggcaagactt taaataaact tatcgcaaat ggaaaattaa gcaaaaaaaa taaaaatttc
    10861 ttacttgatt taatgtttaa taataaaaac ggagacactt taattaaaga tggtgttcca
    10921 aaagactata aggttgctga taaaagtggt caagcaataa catatgcttc tagaaatgat
    10981 gttgcttttg tttatcctaa gggccaatct gaacctattg ttttagtcat ttttacgaat
    11041 aaagacaata aaagtgataa gccaaatgat aagttgataa gtgaaaccgc caagagtgta
    11101 atgaaggaat tttaatattc taaatgcata ataaatactg ataacatctt atagtttgta
    11161 ttatattttc tattatcgtt gacatgtata attttgatat caaaacctga ttttcccttt
    11221 attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca
    11281 aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa
    11341 cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat
    11401 atcaagcaaa gtgacataat tgttgaattt atagtaactg aaacgtatga ttagggtgta
    11461 tattaattat gagatggaaa tcaatgattg ttggctatgc gagagtatca tcgaacgatc
    11521 aaaatttaga aagacaactt gaaaatttaa agacgttttg tgctgaaaaa atatttacag
    11581 aaaaacagtc tgggaaatct attgaaaata gaccagtact tcaagaagca cttaactttt
    11641 tgaggatggg agatcggttt gtagtagaat ccattgattg tttaggtcgt aattatgatg
    11701 aagtggttgg caatccatta ctggataagt ttatgaaaga tttaattatt caaatattag
    11761 caatggtttc agaacaagag agaaatgaaa gtaaacgtcg acaagcgcaa ggaattcaag
    11821 ttgcgaagga aaaaggcgta tataaagggc gccccttact ttattcacca aatgctaaag
    11881 atccacagaa acgtattatt tatcatcgcg ttgtagaaat gttagaagaa ggccaatcaa
    11941 ttagtaaaat tgcgaaagaa gtaaatataa caaggcagac gatttacaga attaaaaacg
    12001 ataatgattt gatttaataa actttgtttc aatgcaactg agagcttctc acagcgtctc
    12061 aaaacagatg attataatct attatgagtt tttaataata agacaagaaa agagagcgta
    12121 aatatgctct ctctttttct cattaacgaa cgtttataac gcaatcatga tgagagtatt
    12181 aatacagtta attatttgaa atactttagt ttatataatg ttgaattata ggtagtgaaa
    12241 ttgtatttat tatacattgt catatcgttg tttaatgata gttaatttaa ggataataga
    12301 attaatatta tatatagatg aacaaaacaa gcaacagagc agaaaaaaca cttttagatt
    12361 atttttactc attctctatt ttatttattt ttgctataat aaataaagaa tattaaatgg
    12421 aggtgttttt atgaaaaaga aatttgtttc tagttgtatt gctagtacaa tcttattcgg
    12481 cactttatta ggtgtaactt ataaagctga agctgcaaca gtacatgtag ctggtggtgt
    12541 ttggagccat ggtattggaa aacattacgt atggtcttat tatagtcaca ataaaagaaa
    12601 tcatggttca acagcagtag ggaaatattc atcttttagt ggtgttgctc gacctggtgt
    12661 tcaatctaag gcatctgctc caaaggcttg gggtggaaac aaaacatttt atagtcttca
    12721 ctaattacat atattaatta aggagaatag acagaattaa gtaaacttaa ttctgtctat
    12781 tctcttgaaa gaggcaatat gaaaaaattc aaattatttt tagattttag tactttatta
    12841 ttaatttcag ggctattatt tttatttttc ttcaaagaaa atgaagaaat tatacctgaa
    12901 agttccaata ttttaacaat ctctaattgg gataaatcta acagtaaatc aaaagtgtta
    12961 gatgtcattg aatcaggcgc caaaaatcaa aatatacaaa taattaaatc cgttaaagat
    13021 tttgataata aaaaagaatt ttttgttttc aactcaaaac gaaataattc tgactttata
    13081 cgtaataaaa catcactttt aactccatca gatttattaa acagagagat aaaaggaaag
    13141 tattatatta ttggtgaaca tttcaatgta gaagagctta cgagtgaatt aaaaaaagct
    13201 ggtttaacat ctgaagttga atacataaat agaaacatat tattttttga attagtcata
    13261 gataatgatt tattagtgcc tttcatattc ttaggcgtat tatatctttt gtacttctta
    13321 tatgacagag gatataattt gaaattttat gctattcaaa agctacatgg cttttcaact
    13381 acacgcatta tttttcataa tattcatata aaaatcattt attggctagt acttaccgct
    13441 attttcttta ttgctaatat attaatcatt catatagcca atttagaatt ggatttctta
    13501 atgtttattc gtagatttat cgtgttgctt tttgtttttg cttgtattac tacattgctg
    13561 tggctgattt cgtatagtct tcttttgaag ttaagtattc cattaatctt aaaagggaaa
    13621 aaaggatata agttttcaat atttatgggt accttcacca aatgtatcat ggtgctaata
    13681 ctatcttttt tactggcaca aaatttaaat gcttatacga agttaaagaa tatatatgac
    13741 tctgaaaaaa tctggcaaaa gttagataat tactatacat tagagatttc tcctaatatt
    13801 agaaattcag aagaaaaaca aattttagaa actaatatat acaatttaat taagaaaagc
    13861 gaacaacttg gtggattgct tttaaagaat aataatatag ccaatcccga taaaaacaat
    13921 tacattcctg aaaatggcaa tgtctttttt atcaataaca attttctgaa tttctataaa
    13981 aatataaatc atgattttaa aatgattgac aatcattcgg ataaaataaa cgtgtttatt
    14041 cctccaaggt tacaatatca aaaatctgaa attcaaaaaa atcaccaagg atggattgac
    14101 tttcaaaagc aacaaaacaa aaaagtagat gtgcaagtac tgtcaaaaaa cgtatctgtt
    14161 ttttcttttg atcgcacaac taatttgaaa tttgggtatc tagattctcc aatagtaatg
    14221 ttgttgacac ttgacgattt aacaggagat ttttatttag ctgctgtttc tcaaggtgga
    14281 tatctattta aagatttaga tacacttaaa cagttgctta aagataatca cctagaagaa
    14341 gaaattagcg gtataactaa ttacaaggat tctgtgttaa atgaattaaa tgaaacgaaa
    14401 acaaaaatga taataacaat agttactatt attattaacg ttttcattct tttgatagct
    14461 actgtatttg aaacaattca atattttagc tggaataaaa agcaactatt attaagaaaa
    14521 attcatggtt attcattatt cagcagtaat gtacgttatt taacaatttc tatcttactt
    14581 tctattatgt tagcttacac tacgcatatt ttatttggtt caaagatatt actattcata
    14641 ataatgtcta ttgctattat tcaacattta cttcaaattg cttatattaa atatcttgaa
    14701 aaatacttta aagatttaat gagggagatt taaacaatga aaagaacttt aattattgta
    14761 acaattgtac tattaatcat catacttagt ctgttattgg ttagaaatga gcatttagat
    14821 cgttttaacc cactgttaaa agaaaaagta tcttatgcca aagttccaaa agatacccag
    14881 gactatagaa atataactgt atactctaaa aatggagaaa aaaagagtta taaattggat
    14941 tttttaggtt atgatcctac aaaagaatat gtgaaagtaa atcataaagg gaaatatgtg
    15001 cgttcgatag aatacattac aaaagatatt cctagctttt taaaatgagg tgaccgatag
    15061 tgataaaact tgaaaatgtt tttgtaaaaa aaggtaataa aaacatttta gacggatgta
    15121 actttaattt tgaaaaagga aaatcatatg ctttagttgg agaaagtggc gctggaaaat
    15181 ctactttact taacataatc gctgggttcg aagatgtatc acaaggaagc atatatattg
    15241 aagataagtt actaaaaaag aaagtcgatt tttatagata tacgttaggg tatttgtttc
    15301 aaaattttgc tttacttgaa aatcaaacaa tcagtcaaaa tttagattta gccctaaagt
    15361 tcaaaaagaa taaaaaagat aatatgaact taaagaaaga agtattaaaa aaggtcggcc
    15421 tagatttaga tatcaagaga attgttagtt ctctaagcgg tggtgaacaa caaagagtag
    15481 cattggcaag attaatatta aaagatccga aaatcatttt agctgatgaa cctactgggt
    15541 cactagatac gaaaaatgga aaaatagtta ttgatttatt attgaagcta cttgatgaaa
    15601 ataaaactat gattgtcgta acgcatgatt tagagcttgc gaaacgattt gatgtcatag
    15661 tcaatatttc tgagcttaga agttaagaaa tattgatagg tatagccata catagagaaa
    15721 aaacaatatg aaatatgatt agtcattact tttgagtttt ttaatttaag gactggattt
    15781 tccagtcctt ttttatgatg acaaaaaaca agcctcgcag agcacacgtt tttaatgact
    15841 tatttggttt ttaataagtc tagtatgtta aaacttttct gtgtttaggt gttataatca
    15901 tatgcagtgg aagttgattt attatgtctt attcaattat tcgagttgtt aaagtgaaat
    15961 caaaaacaaa tacaagaggt attcaaagac atattcaaag agaaaataaa aattatgaaa
    16021 atatagatat tgatttaagt aagtcatatt taaactatga tttagtcaat gatactaagt
    16081 ttgattttaa taaaaaaatt gatgagaaaa tcgaaaaaaa ttataaaggg aaacgcaaaa
    16141 ttagaacaga tgccattaaa catattgatg gattaattac atcagataat gtgtttttta
    16201 atcaattatc tgaagaagag acaaaatagt tttttaagca tgctaaagcg ttcttggaag
    16261 aagaatacgg taaagaaaat cttttatatg cgacagtaca tatggatgag ataacaccac
    16321 atatgcatta tggcgtagtt cctattacta aagatggacg tttaagtgcg aaagaagttg
    16381 taggtaataa aaaagcatta accgaattcc aagatagatt caatacgtat attaataagc
    16441 aaggctatga tttaaaacga ggaatttcta gacaacttac aaaagaaaaa catgatcaag
    16501 ttagccgtta taaacaaaaa acagaatatc ataaacaaat gcatatgcgc tagaagcaaa
    16561 tagaggatta tttaaagcaa gaaaatgata aattgataca aaactaccaa aaatcattag
    16621 atacgcttaa aaagcttctg agtgtcatgt atgaaattaa aactaaaaat gtaggtggat
    16681 ggtttcataa agagaagcaa gaaacaggca atattgtaat tactaaaact gattttgaaa
    16741 aatacactaa acaaattaaa gcagcacaga tgatattaga tgattatgaa tgtattaaaa
    16801 gtgggaagtc attaaaaaaa gcagagaaac aaaatgaaag tttagttaat gaattgacta
    16861 gtgtccatat ggaaaatgaa aagctggttg aagaatttaa tgatttagca caacgttata
    16921 attatttgtt gtcagagaat gaaaagaaag ataaagaact aaattatacg cttaagttat
    16981 tcaatcaagt ttttaaaatc attaaaagta tgatgaaaga agagcgttat catacactaa
    17041 taaatcatat tgataatcat ttagacaatt ctaaaatacg tgaagtaatg acgatagata
    17101 acaatgatga acagttcttt aagaagaaat atcaagcaca agaacgagaa atcattttta
    17161 aagaagatag agaagatggt tatacattat aaaaatagat atttaaatgc atttgtttga
    17221 ggttaattat tttttaggat gaaaaatagg tagcccccct cgaacttttt aatatatttt
    17281 tataaatctt tttaaaaacc tttttagggg cttgttcagc cctagagcaa caagggattt
    17341 caatgcctaa aggagtgttt ttgctacata aaaggagtgt ttttgccaca taaaaggagt
    17401 gtttttgcca cataaaagga gtgtttttgc cacataaaag gagtgtttta aaaagttgta
    17461 ctccttttat ggtttgacat tgataataaa gtagtttatt gtgaataaaa gttttcaaat
    17521 gggagtgtat attcaaacga tactccttta ggttcgtaat aaagggggct gtatcatgac
    17581 aggagagaca gttgtatata aaaatgaaat gaatttagtt ccattaaggc gatttacagc
    17641 tactgaaatt aatttatttt ttgcgatgtg taataaatta aaagagcaag atactaatac
    17701 attacgtttg tcttttgatg aattaaaaaa attaagtaat tatagtccag aaacacgtaa
    17761 tattaataga tttgctaatg atttagataa tgtgtataaa aagatgttga atttaaccat
    17821 tagatatgaa gatgatgatg taatagaaag attcgtttta ttcaatcatt atagaatcca
    17881 taagcgagaa caatatctag aaatatcaac ttcttcaaat ttaaaacaca tattgaattc
    17941 aattactaat aattttacaa aatttgaatt aaaagaaatg actaggctta aatctacata
    18001 ttctaaaaat atgtttagat tacttaaaca atacaaacat acaggctatt taaaaattca
    18061 catcgatgat tttaaaaatc gtttagatat acctaaatct tatcgtatga ctgatattaa
    18121 caaaaatgtt tttaaaccta ttattattga attaggttct atatttaata atcttactat
    18181 caataaaatc aaagcgaaaa aagggcgtaa aattgaatgg atagagttca catttgacgc
    18241 tgagaaacgc attcataaca aacgacaacc tcaaatgtcc aagatagata aatcaagaca
    18301 atatgtgagg cgtgaaaaga cgcctaaatg gttagaagaa cggtcatatg aaaagcaacc
    18361 tcaaaaagat tacgacccac aattagagaa agaacgagag gattttttaa aacaacttga
    18421 actgaattgg gaataacgca acttttcgtt gcgagttggt taactaaaag tatcgtgact
    18481 tttcaattat tttaattata gtttgagtta aggagtgatt ttatgaaatt agctgaagct
    18541 ataaaagaac agagagaatt aaaaggatgg tctcaagagg aattagccaa tatattaaag
    18601 gtttccagac aaagtgtttc taaatgggaa agtgccaaaa actatccttc cttagatatt
    18661 ttgattgcta tgagcgattt attcggaatc tctttagagc atttaattaa aggagatgca
    18721 cgttttaaaa aagttatttt agagggaaat tatagagaga gtaacagggt accttccata
    18781 gttgattttc tttatgattt ttggtggctt ttctttcctg ttgccggatt tttagtttgg
    18841 gccattcatt cgttcatggg ttgattttaa aatcaaaatt taatgcacat caggcaatat
    18901 gaaaaaggaa agtattttta cgacaccttg aaatgtattg ataagaatag aagagtttac
    18961 aaaaggcaat atgaaatata tactaaaagt aacaagaatt tcgtttgtaa acgcaccaaa
    19021 ccccgaacta ttcgccgtag ttcggggttt tttggtgaat tggctttaat cgtctatatt
    19081 attttttatc acgtttatgg agctaataac caaataacgt aacgagacaa ccactgattg
    19141 ccgttgtcat gatgtgaaca agaatttcat tcataaatcg cacctcctct ctacgccgaa
    19201 ttgacatctg agagatagac gaccttcaca ttataacaaa tctctccttc catgaaataa
    19261 catgaatatt aataaatgtt gttatatatt aaagataaat aaatataagg gtgggattga
    19321 taatgtttgg cgatcaaaga caagaagcta caaaatatgt cattaaagaa ggttatcaag
    19381 atatttattt cctcaacaaa aacggggaat ggtattattt cgaagtaaga agtgtctggc
    19441 gtggcaaaca tattattaga gttaaagatg gtttgttagg ttggagaaaa gaaatagtaa
    19501 ctgaataaag atacaaaata cttgccaatg caaaagaatg aaaacgcgtc aaatcaacga
    19561 tgtataaaag atgcataaaa gataatgaaa gtaaaaagcc ataaatgccg tcatataggc
    19621 atttatggct ttttgtttgg ttaatataat cgatgattat cagaagttca attcacataa
    19681 aaaagcacgg ctaatttatg aacttgtcgt acctttttaa ttaaaattag ttaccaaata
    19741 gacgactcca aaaccccttt ttagcttccg tttcttcact cttttcttct atatcactat
    19801 cattgtcatt tggattagct gaaacgtctt ctctttgtgc ttcttcacct tgtgtacttt
    19861 cgtcgctttg acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct
    19921 gatctcggtt aatatttaca ggactagtag tatatgtttt ttcttgtgca tcaacgtttt
    19981 gtctagcgtt agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat
    20041 tttctaattt ttgaatcttt ttattactct ctaatgccag aacttgctga ttctcaagca
    20101 attttgatct ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt
    20161 cttgtttttc aatttgatta tttaaagtat taatctgatt tttaagtatt tctactattt
    20221 gagcatcgct tttagattca aaattaatat tatccgactc ttttttttgt ttgtcttcag
    20281 tagcactttc aaaaccatat ttctttttat tttcatttac tctttgaatg attttttcta
    20341 tatcggtatc ttctttaata aacgaagtgt tttcctgttt tattgtctct atattcaatc
    20401 tcttaatatt attaaaaatc gtttgcttac taacccctaa ttcttcactc aaccttttta
    20461 cacttttcat atcatcaacc ctttataaaa tgtttataaa cccttagtaa aatattataa
    20521 gcttataacg attaataata aagtaatcga caaataaaaa tataaattcg ccaacctttt
    20581 tatcaagggt ttgtaaaggg tttatggtgt ttatcaaagg tttataaacc tatattttcc
    20641 cttttttaag ttt
//
