LOCUS       NZ_MH785246             3605 bp    DNA     circular CON 21-JUL-2023
DEFINITION  Staphylococcus aureus strain ph1 plasmid pPH1-3, complete sequence.
ACCESSION   NZ_MH785246
VERSION     NZ_MH785246.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09846913
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Bukowski,M., Piwowarczyk,R., Madry,A., Zagorski-Przybylo,R.,
            Hydzik,M. and Wladyka,B.
  TITLE     Prevalence of Antibiotic and Heavy Metal Resistance Determinants and
            Virulence-Related Genetic Elements in Plasmids of Staphylococcus
            aureus
  JOURNAL   Front Microbiol 10, 805 (2019)
  REMARK    DOI: 10.3389/fmicb.2019.00805
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Bukowski,M., Hydzik,M., Sabat,A.J. and Wladyka,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) Faculty of Biochemistry, Biophysics and
            Biotechnology, Department of Analytical Biochemistry, Jagiellonian
            University, Gronostajowa 7, Krakow, malopolskie 30-387, Poland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/21/2023 01:07:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MH785246.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ph1"
                     /isolation_source="tenosynovitis"
                     /host="Farmed pheasant"
                     /db_xref="taxon:1280"
                     /plasmid="pPH1-3"
                     /geo_loc_name="United Kingdom: Scotland"
     gene            220..636
                     /gene="mobC"
                     /locus_tag="D0Y80_RS00005"
                     /old_locus_tag="D0Y80_n00005"
     CDS             220..636
                     /gene="mobC"
                     /locus_tag="D0Y80_RS00005"
                     /old_locus_tag="D0Y80_n00005"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_172693035.1"
                     /translation="MIALGGKFDGTFLPSPPKLDEKGVRFLATENRRESKQIKFRVTQE
                     EYERLEQMAETLNTSVPMLAKDAAQKKRIKAPLIDRQGAIEIAKELRAIGTNINQITRA
                     VNTKDLIDYDIGNVLLDELGDLRNEMGKLWQLLK"
                     /name="mobC"
     gene            618..1727
                     /locus_tag="D0Y80_RS00010"
                     /old_locus_tag="D0Y80_n00010"
     CDS             618..1727
                     /locus_tag="D0Y80_RS00010"
                     /old_locus_tag="D0Y80_n00010"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_172693036.1"
                     /translation="MAVIKISATKSLNRLISYAEKXAEXQSGVNVDDEFAKEQMKTTRM
                     IFNKNDGRQGYHLIQSFDPEDNITPEEANEIGQELAKEVAPDHEITIYTHTDRDHIHNH
                     ICINSVNMNTGKKFVNDRNKLYEMRNKSNELCQRYNLINSSNKQAQIRYTQAERAIIEK
                     GQVSWKDEIRQAIDKEKLNSNSFDEFKDNMQENYGIEVKERGKNISFKHPDKKAFVRGK
                     TLGNAYEKGTINNELNRESERTEQQTQSDRPKQVTRTNERSRGTDKDIEKRADVTRIEH
                     YSRDNGDTQSVGQDTKPNRKGTKRNSKRERDFRRAFKKHNERTAELNGRADEELPDREQ
                     ENVRDNQADIGEDRAEPQLEIELDGPELQ"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2043..2225
                     /locus_tag="D0Y80_RS00015"
                     /old_locus_tag="D0Y80_n00015"
     CDS             2043..2225
                     /locus_tag="D0Y80_RS00015"
                     /old_locus_tag="D0Y80_n00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172693037.1"
                     /translation="MLGMNDDTKELVVKELEYINGSVEELKAFLDRYDDETIIGRIVEI
                     EARTQKIFNNIQKEH"
                     /name="hypothetical protein"
     gene            2677..2838
                     /locus_tag="D0Y80_RS00020"
                     /old_locus_tag="D0Y80_n00025"
     CDS             2677..2838
                     /locus_tag="D0Y80_RS00020"
                     /old_locus_tag="D0Y80_n00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019835937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165628176.1"
                     /translation="MENKKERKRLNITLPPELIEELEKVNKKYKINKSSQIETLITKYL
                     RKEYGDLG"
                     /name="hypothetical protein"
     gene            2920..3489
                     /locus_tag="D0Y80_RS00025"
                     /old_locus_tag="D0Y80_n00020"
     CDS             2920..3489
                     /locus_tag="D0Y80_RS00025"
                     /old_locus_tag="D0Y80_n00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_069996713.1"
                     /translation="MAKYTKSRTWGMVVYPESAPENWQELLEETFMQFAVSPLHDKDTN
                     PDGEIKKPHWHVILIWDGPVTQNAAMKIAEKVNAPQPVKLESVRGAYRYFTHMDNPEKY
                     QYDEKDIKLYNGFDISSYVSLTKEEKYEAIGKIMDIINDNGITEYIDLLNILRMNDYNL
                     FKVACDNTILFTNVVRSLRHSEDKRR"
                     /name="replication protein"
     Typing          2921..3489
                     /name="rep_cluster_2342"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          619..1724
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.748"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttattcagtc cctttttgcg ttagcaaaaa cataaagggt ttgggatata atcccaacaa
       61 gtaggtgtat gggtaccata cacgtatctt gccaaatccc tctcatcaat gctatgatga
      121 ggccgtaaag ttgtttattt aattggtgcc gacaattaaa taaatcaaca tacgggcgtt
      181 ttttagggtg gcgcaaagat taagcgccag actaaaaaca tgatagcttt agggggcaaa
      241 tttgacggca catttttgcc gtcgccccct aaacttgatg agaaaggggt gcgatttttg
      301 gctacggaga atagacgaga aagcaagcaa ataaagttca gagtgacaca agaagaatat
      361 gagcgacttg aacaaatggc agaaacgtta aatacatctg ttccaatgct tgcaaaagac
      421 gcagcgcaga aaaagcgtat aaaagcacca ttgattgata gacaaggtgc aattgaaatt
      481 gcgaaagagt taagagcaat tggaacaaat attaatcaga ttacaagagc agtaaataca
      541 aaagatttaa ttgattatga cataggaaac gtattattag acgaattagg cgatttaaga
      601 aacgagatgg gtaaattatg gcagttatta aaataagcgc tacaaagtct ttaaataggc
      661 ttatatcgta tgccgaaaag waagcagaar wacaatcggg tgtaaatgtt gatgatgagt
      721 ttgcgaaaga gcagatgaaa acaacacgaa tgatatttaa taaaaacgac ggacgacagg
      781 gttatcattt aattcagtca ttcgatccag aagataatat cacaccagaa gaagccaatg
      841 agataggtca agaattagca aaagaagttg caccagacca tgaaataacg atttatacgc
      901 atacagatag agaccatatt cataatcata tatgcataaa tagtgtgaat atgaatacag
      961 ggaaaaaatt tgtgaatgat agaaataagc tttatgaaat gcgtaataaa agtaatgagt
     1021 tatgccaaag atacaattta attaattcta gcaacaaaca agcacaaatt aggtacacgc
     1081 aggcagaaag agcaattata gaaaaaggtc aagttagttg gaaagatgaa attagacaag
     1141 cgattgataa agaaaaacta aattcaaata gttttgatga atttaaagat aatatgcaag
     1201 agaactacgg tattgaagtt aaagaacgtg gaaagaatat cagtttcaaa catcccgata
     1261 aaaaagcatt tgttagaggt aaaactttag gcaatgctta cgagaaagga acgataaata
     1321 atgagcttaa cagagagagt gaacgaacag aacaacagac gcaatcagat agaccaaaac
     1381 aagttactag aacaaatgaa agatctagag gcacagataa ggacattgag aaacgagccg
     1441 atgttactag aatcgagcat tactcaagag ataacggcga tacacaaagc gtcggacaag
     1501 atactaaacc aaatcgcaaa ggcacaaaaa gaaatagcaa gcgagaacga gacttccgta
     1561 gagcatttaa aaagcataat gaacgcactg cagaacttaa tggacgagca gatgaagaac
     1621 ttccagatag agaacaagaa aatgttagag acaatcaagc cgacattgga gaagatagag
     1681 cagagccaca actcgaaatt gaactcgatg gaccagaact tcaatagaat ggttatgcat
     1741 atacgtcaca caagcaatat gaataacctt agaaaccatt taatcaatat tgggggttca
     1801 agtatcctaa caacgatatt agtccttatt ttgcatttta taggggttgc atgagccaca
     1861 taaatgatta agcacgtttt atgccgagaa aatctatttg tcgatgagag cccttaatta
     1921 gactaaaagc gccagcggtc ggatagcgtc agctatcaaa tcgaccgatg gacagcttta
     1981 ggattattaa ggagcgcaga atcgtcggca aatagaggaa ttggaataaa catataaagg
     2041 gagtgcttgg aatgaatgat gatacaaaag aattagtagt taaggaatta gaatatatta
     2101 atggttcggt tgaagagtta aaagcttttt tggatagata tgatgacgaa acgataattg
     2161 gccgtattgt agaaatagag gctagaacgc aaaagatttt taataatata caaaaagagc
     2221 actgatcctt tttgggtcag tgctctttta tagcatattg tcctactaac aggataactc
     2281 gtgcatttga attttaacgt tcgcgcagag tcgacaaacg acagacaggt caattgccag
     2341 ttagtacaaa cgccgacctt ccgtaagcgt tatggcatta ctcacacgca ttaattttta
     2401 tatctgccac gagtaactat ggccgattct gcaatatgct attagtatgt tataacatga
     2461 tatgaaatgt catatcaatt taagtgtttt ttggtttttg ggttagtatt ttttttgagg
     2521 ttgtttcaca tattttgggg tgttttccta ggggggtact acgacccccc ctaggagtgc
     2581 attgtgcatt gtgaaaaata atgagcatat tattgacttt atgcgcatag atatgtatat
     2641 tgaacacata agcaattttg gaggtgtttt ttgatattgg agaataaaaa agagagaaaa
     2701 agattaaata ttacattacc gccagagcta attgaggaat tagaaaaagt gaataaaaaa
     2761 tataaaataa ataagtctag tcagattgaa acgttgatca ctaaatattt aagaaaggag
     2821 tatggtgatt taggataaaa aaaagaacct tgccctaaca agattctttt tcgatgatgt
     2881 catgacgtga catgttttgt taatcaccaa tataatacca tggctaaata cactaaatca
     2941 agaacgtggg gtatggttgt ttacccagaa tcagcccccg aaaattggca agaattatta
     3001 gaggaaactt ttatgcaatt tgcagtttct cctttgcatg ataaagatac aaatccagat
     3061 ggagaaataa aaaaaccaca ttggcatgta atattaatat gggacgggcc agttactcaa
     3121 aatgcagcaa tgaaaatagc tgaaaaagta aatgcacctc aacctgttaa attagaaagc
     3181 gtgcgtggtg catatagata tttcactcac atggataacc ctgaaaaata tcaatacgat
     3241 gaaaaagata taaaattata taatggcttt gatatatctt cttatgtgtc attaacaaaa
     3301 gaagaaaagt atgaggctat tggaaaaata atggatataa ttaacgataa tggaattaca
     3361 gaatatatag atttattaaa tatacttaga atgaatgact ataatttatt taaagttgct
     3421 tgtgataaca ctatattgtt tacaaatgtt gtacgttcgc ttagacattc agaagataag
     3481 cgaagatgat tttgaactta gagaatttaa ttttattcta cgagtttaaa cgagtagatc
     3541 ataaaattaa atgaacatat agcaaccata tttttggttg ctgggttttg aactttggga
     3601 ctgat
//
