LOCUS       NZ_MH785245             2036 bp    DNA     circular CON 21-JUL-2023
DEFINITION  Staphylococcus aureus strain ch8 plasmid pCH8, complete sequence.
ACCESSION   NZ_MH785245
VERSION     NZ_MH785245.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09846907
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Bukowski,M., Piwowarczyk,R., Madry,A., Zagorski-Przybylo,R.,
            Hydzik,M. and Wladyka,B.
  TITLE     Prevalence of Antibiotic and Heavy Metal Resistance Determinants and
            Virulence-Related Genetic Elements in Plasmids of Staphylococcus
            aureus
  JOURNAL   Front Microbiol 10, 805 (2019)
  REMARK    DOI: 10.3389/fmicb.2019.00805
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Bukowski,M., Hydzik,M., Sabat,A.J. and Wladyka,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) Faculty of Biochemistry, Biophysics and
            Biotechnology, Department of Analytical Biochemistry, Jagiellonian
            University, Gronostajowa 7, Krakow, malopolskie 30-387, Poland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/21/2023 01:11:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MH785245.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ch8"
                     /isolation_source="synovial fluid"
                     /host="Broiler chicken"
                     /db_xref="taxon:1280"
                     /plasmid="pCH8"
                     /geo_loc_name="USA: Georgia"
                     /collection_date="1999"
     gene            99..245
                     /locus_tag="D0Y74_RS00005"
                     /old_locus_tag="D0Y74_i00005"
     CDS             99..245
                     /locus_tag="D0Y74_RS00005"
                     /old_locus_tag="D0Y74_i00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017176832.1"
                     /translation="MKFGNYKIDSFWLIMIIGFLATSIFFPFMLLSVIILLIFGLEKDD
                     KKG"
                     /name="hypothetical protein"
     gene            464..1468
                     /locus_tag="D0Y74_RS00010"
                     /old_locus_tag="D0Y74_i00015"
     CDS             464..1468
                     /locus_tag="D0Y74_RS00010"
                     /old_locus_tag="D0Y74_i00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_107523773.1"
                     /translation="MHFYTTGYIDENQDNETLKDMTKSGKQRPWKEKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQRVI
                     GEVVKEKPKARWLFLTLSTRNAIDGDTLEQSLKHLTKAFHRLTKYKKVSKNLVGFMRST
                     EVTVNENDGSYNQHMHVLLCVENAYFRKKENYITQTEWVDLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDERNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     Typing          465..1468
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.219"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaagttttg ggattgttaa gggttccgag gctcaacgtc aataaagcaa ttggaataaa
       61 gcaattatgt tattttgatt aaaaaaaggg gggcttgtat gaagtttgga aattataaaa
      121 ttgattcttt ttggttaatt atgattatag gttttttagc tacttctata ttttttcctt
      181 ttatgctttt gtctgtaatt attttattga tttttggttt agaaaaagat gataaaaaag
      241 gttaaatttg atttcttgat tttggttttt gaaacgagtt gaaaacgagt ttcttcttgt
      301 cttgatacta tatagaaata acaatctttt tgaaattgct cgtcaaatgt tgatgtgaga
      361 gtgtttgagc tgttttttat gcgtgaaaga aatttttgac ataaagaaac ccctgtatta
      421 agatttaatt gtctagaaaa aaccaaatac agggggctct tatatgcatt tttatactac
      481 aggatatata gacgaaaatc aagataatga aacattaaaa gatatgacta aaagtgggaa
      541 acaacgccca tggaaagaaa agaaaattga taatgtgagc tatgcagata tactggaaat
      601 tttgaaaatc aaaaaggctt ttaacgtaaa acaatgtggt aacgtcttag agttcaagcc
      661 gactgatgaa ggttatttga agttacataa gacatggttt tgtaaatcaa aattatgccc
      721 agtttgtaat tggaggcgtg ctatgaaaaa tagttatcaa gctcaaagag taattggaga
      781 agtggttaaa gaaaaaccga aagcgcgttg gttgttttta actctttcaa caagaaatgc
      841 gatagacgga gatactttag aacaaagttt aaaacattta acaaaagctt ttcatagatt
      901 aactaaatac aaaaaagtta gtaagaattt agttggtttt atgcgttcaa ctgaagttac
      961 agttaatgag aatgacggaa gttataatca acatatgcat gttttgttat gtgttgaaaa
     1021 tgcgtatttt agaaaaaaag aaaattatat tacgcagact gaatgggtcg atttatggca
     1081 aaaagcatta caagtaaatt atcgacccgt agcaaatatt aaagcgatca aaccaaatca
     1141 aaaaggcgat aaagatattc aagcagctat caaagaaacc tcgaaatatt cggttaagtc
     1201 atctgatttt ttaactgatg atgacgaaag aaatcaagaa atcgtgaatg atttagaaaa
     1261 aggtttatac cgaaaacgta tgttgagtta tggtggattg cttaaacaaa aacacaaaat
     1321 tttaaattta gatgatgccg aagatggcaa tttaattaat acaagtgacg aagataaaac
     1381 aacagacgaa gaagaaaaag cacattcaat tactgcaatt tggaattttg aaaaacaaaa
     1441 ttattactta aaagatttga aacgttagct taaaagctag cgtttttttt atgctttttg
     1501 cccctgcggg aactatagga ttaaccagca tggttaccca gtttttacgc gaaaaataat
     1561 tttgtgcgta actgggcagt gtctgaattt ttttgccagt acatgccaaa tttttaattt
     1621 gcatgtaact gggcagtgtc taaaaaatca ttcactggtt ttgatcaaat tttagttggt
     1681 gtaattaaaa tatatttatt tggctcatat ttgcgtttta agagcttata tacgttttta
     1741 ggtataaaac tatatgattt acccctaaat ctttaaaatg cccccttaaa attcaaaata
     1801 aaggcattta aaatttaaat agttcttgtg ataaagtttg ctaaaaagga gatagaaaat
     1861 gcttatattt ttatttattt tggtttgtgg tcttttaggt tattttttga ttaatcgttc
     1921 taaaaaagat tagacatgca tttatgccga gaaaatttat tgtgcgttga gaagaaccct
     1981 taactaaact tgcagacgaa tgtcggcata gcgtgagcta ttaagccgac cattcg
//
