LOCUS       NZ_MH785230             4440 bp    DNA     circular CON 21-JUL-2023
DEFINITION  Staphylococcus aureus strain ch8 plasmid pSAP060B, complete
            sequence.
ACCESSION   NZ_MH785230
VERSION     NZ_MH785230.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09846907
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Bukowski,M., Piwowarczyk,R., Madry,A., Zagorski-Przybylo,R.,
            Hydzik,M. and Wladyka,B.
  TITLE     Prevalence of Antibiotic and Heavy Metal Resistance Determinants and
            Virulence-Related Genetic Elements in Plasmids of Staphylococcus
            aureus
  JOURNAL   Front Microbiol 10, 805 (2019)
  REMARK    DOI: 10.3389/fmicb.2019.00805
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Bukowski,M., Hydzik,M., Sabat,A.J. and Wladyka,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) Faculty of Biochemistry, Biophysics and
            Biotechnology, Department of Analytical Biochemistry, Jagiellonian
            University, Gronostajowa 7, Krakow, malopolskie 30-387, Poland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/21/2023 01:08:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MH785230.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ch8"
                     /isolation_source="synovial fluid"
                     /host="Broiler chicken"
                     /db_xref="taxon:1280"
                     /plasmid="pSAP060B"
                     /geo_loc_name="USA: Georgia"
                     /collection_date="1999"
     gene            join(3241..4440,1..42)
                     /gene="mobV"
                     /locus_tag="D0Y74_RS00005"
                     /old_locus_tag="D0Y74_e00000"
     CDS             join(3241..4440,1..42)
                     /gene="mobV"
                     /locus_tag="D0Y74_RS00005"
                     /old_locus_tag="D0Y74_e00000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            569..1513
                     /locus_tag="D0Y74_RS00010"
                     /old_locus_tag="D0Y74_e00010"
     CDS             569..1513
                     /locus_tag="D0Y74_RS00010"
                     /old_locus_tag="D0Y74_e00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1675..3054
                     /gene="tet(K)"
                     /locus_tag="D0Y74_RS00015"
                     /old_locus_tag="D0Y74_e00015"
     ARG             1676..3051
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          570..1513
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3242..4419
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 caacaaaaga atcaaaaacg agatagaggt atgcacttat agaacatgca tttatgccga
       61 gaaaacttat tggttggaat gggctatgtg ttagctaact tgttagcgag ttggttggac
      121 ttgaattggg attaatccca agaaagtacc aactcaacaa cacataaagc cctgtaggtt
      181 ccgaccaata aggaaattgg aataaagcaa taaaaggagt tgaagaaatg aaattcagag
      241 aagcctttga gaattttata acaagtaagt atgtacttgg tgttttagta gttttaactg
      301 tttaccagat aatacaaatg cttaaataaa aaaagacttg atctgattag accaagtctt
      361 ttgatagtgt tatattaata acaaaataaa aaggagtcgc tcacgccctg accaatgttt
      421 gtgaacgaca tcattcaaag aaaaaaacac tgagttgttt ttataatctt gtatatttag
      481 atattaaacg atatttaaat atacatcaag atatatattt gggtgagcga ttccttaaac
      541 gaaattgaga ttaaggagtc gattttttat gtataaaaac aatcatgcaa atcattcaaa
      601 tcatttggaa aatcacgatt tagacaattt ttctaaaacc ggctactcta atagccggtt
      661 ggacgcacat actgtgtgca tatctgatcc aaaattaagt tttgatgcaa tgacgatcgt
      721 tggaaatctc aaccgagaca acgctcaagc cctttctaaa tttatgagtg tagagcccca
      781 aataagactt tgggatattc ttcaaacaaa gtttaaagct aaagcacttc aagaaaaagt
      841 ttatattgaa tatgacaaag tgaaagcaga tagttgggat agacgtaata tgcgtattga
      901 atttaatcca aacaaactta cacgagatga aatgatttgg ttaaaacaaa atataataag
      961 ctacatggaa gatgacggtt ttacaagatt agatttagcc tttgattttg aagatgattt
     1021 gagtgactac tatgcaatgt ctgataaagc agttaagaaa actatttttt atggtcgtaa
     1081 tggtaagcca gaaacaaaat attttggcgt gagagatagt aatagattta ttagaattta
     1141 taataaaaag caagaacgta aagataatgc agatgctgaa gttatgtctg aacatttatg
     1201 gcgtgtagaa atcgaactta aaagagatat ggtggattac tggaatgatt gctttagtga
     1261 tttacatatc ttgcaaccag attggaaaac tatccaacgc actgcggata gagcaatagt
     1321 ttttatgtta ttgagtgatg aagaagaatg gggaaagctt cacagaaatt ctagaacaaa
     1381 atataagaat ttgataaaag aaatttcgcc agtcgattta acggacttaa tgaaatcgac
     1441 tttaaaagcg aacgaaaaac aattgcaaaa acaaatcgat ttttggcaac atgaatttaa
     1501 attttggaaa tagtgtacat attaatatta ctgaacaaaa atgatatatt taaactattc
     1561 taatttagga ggattttttt atgaagtgtc tatttaaaaa tttggggaat ttatatgagg
     1621 tgaaagaata atttacccct ataaacttta gtcacctcaa gtaaagaggt aaaattgttt
     1681 agtttatata aaaaatttaa aggtttgttt tatagcgttt tattttggct ttgtattctt
     1741 tcatttttta gtgtattaaa tgaaatggtt ttaaatgttt ctttacctga tattgcaaat
     1801 cattttaata ctactcctgg aattacaaac tgggtaaaca ctgcatatat gttaactttt
     1861 tcgataggaa cagcagtata tggaaaatta tctgattata taaatataaa aaaattgtta
     1921 attattggta ttagtttgag ctgtcttggt tcattgattg cttttattgg tcacaatcac
     1981 ttttttattt tgatttttgg taggttagta caaggagtag gatctgctgc attcccttca
     2041 ctgattatgg tggttgtagc tagaaatatt acaagaaaaa aacaaggcaa agcctttggt
     2101 tttataggat caattgtagc tttaggtgaa gggttaggtc cttcaatagg gggaataata
     2161 gcacattata ttcattggtc ttacctactt atacttccta tgattacaat agtaactata
     2221 ccttttctta ttaaagtaat ggtacctggt aaatcaacaa aaaatacatt agatatcgta
     2281 ggtattgttt taatgtctat aagtattata tgttttatgt tatttacgac aaattataat
     2341 tggacttttt taatactctt cacaatcttt tttgtgattt ttattaaaca tatttcaaga
     2401 gtttctaacc cttttattaa tcctaaacta gggaaaaaca ttccgtttat gcttggtttg
     2461 ttttctggtg ggctaatatt ttctatagta gctggtttta tatcaatggt gccttatatg
     2521 atgaaaacta tttatcatgt aaatgtagcg acaataggta atagtgttat ttttcctgga
     2581 accatgagtg ttattgtttt tggttatttt ggtggttttt tagtggatag aaaaggatca
     2641 ttatttgttt ttattttagg atcattgtct atctctataa gttttttaac tattgcattt
     2701 tttgttgagt ttagtatgtg gttgactact tttatgttta tatttgttat gggcggatta
     2761 tcttttacta aaacagttat atcaaaaata gtatcaagta gtctttctga agaagaagtt
     2821 gcttctggaa tgagtttgct aaatttcaca agttttttat cagagggaac aggtatagca
     2881 attgtaggag gtttattgtc actacaattg attaatcgta aactagttct ggaatttata
     2941 aattattctt ctggagtgta tagtaatatt cttgtagcca tggctatcct tattatttta
     3001 tgttgtcttt tgacgattat tgtatttaaa cgttctgaaa agcagtttga atagttatat
     3061 tatattttgg tttagaacta tgagtggcta gcattttgcc actcattttt tgcgttagca
     3121 aaaacaggtt taagcctcgc agagcacacg tattaacgac ttattgtaaa ataagtctag
     3181 tatattagac ttaaactatt aaatacacat gaaacctttg tgcttaggag tgatttttat
     3241 atgtcttatt ccattgttag agtttcaaaa gttaaatctg gaacaaatac aacgggcata
     3301 caaaaacatg ttcaaagaga aaataataat tatgaaaatg aagatataga ccatagtaaa
     3361 acttacttaa attatgattt ggtaaatgct aataaacaga attttaataa cttgattgat
     3421 gaaaaaatcg aacagaatta tacaggcaaa agaaaaatta gaacagacgc gattaaacac
     3481 attgatggtt taattacatc agacaatgat ttctttgata atcaaacgcc agaagataca
     3541 aagcagtttt ttgaatatgc taaagagttt ttagaacaag aatacggtaa agataattta
     3601 ttatatgcaa cagttcacat ggacgaaaaa acaccacata tgcattatgg cgttgttcca
     3661 ataactgatg atggtcgttt aagtgctaaa gaagttgtag gtaataaaaa agctttaaca
     3721 gcgtttcaag atagatttaa tgagcatgtt aaacaacgag gatatgattt agaacgtggg
     3781 caatcaagac aagtaacaaa tgctaaacat gagcaaataa gtcagtataa acaaaaaaca
     3841 gaatatcata agcaagaata tgaacgtgag agccaaaaaa cagaccatat aaagcaaaag
     3901 aacgataaat taatgcaaga gtaccaaaaa tcgttaaata cgcttaaaaa gcctataaat
     3961 gttccgtatg agcaagaaac tgaaaaagta ggtggtttat ttagcaaaga aatacaagaa
     4021 actggaaatg ttgtaataag ccaaaaagat ttcaatgaat ttcagaaaca gataaaagct
     4081 gctcaagata tttcggaaga ttacgagtat ataaagtctg gtagagcctt agatgataaa
     4141 gataaggaaa tacgagagaa agatgattta ttaaataaag cagttgagcg tattgaaaac
     4201 gcagacgata attttaacca actttacgaa aatgcaaagc cacttaaaga gaatatagaa
     4261 atagcgttaa agcttttaaa aatcttacta aaagagttag aacgagtttt aggaagaaat
     4321 acctttgcgg aaagagttaa taagttaaca gaagatgaac caaaactaaa tggtttagca
     4381 ggaaacttag ataaaaaaat gaatccagaa ttatattcag aacaggaaca gcaacaagaa
//
