LOCUS       NZ_MH251947             2220 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Staphylococcus aureus subsp. aureus ECT-R 2 isolate Lancaster/2015/3
            plasmid pLUH01, complete sequence.
ACCESSION   NZ_MH251947
VERSION     NZ_MH251947.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14228209
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus subsp. aureus ECT-R 2
  ORGANISM  Staphylococcus aureus subsp. aureus ECT-R 2
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Atkinson,K.V., Bishop,L.A., Rhodes,G., Salez,N., McEwan,N.R.,
            Hegarty,M.J., Robey,J., Harding,N., Wetherell,S., Lauder,R.M.,
            Pickup,R.W., Wilkinson,M. and Gatherer,D.
  TITLE     Sequence Variation in Multidrug-Resistant Plasmid pLUH01, Isolated
            from Human Nasopharyngeal Swabs
  JOURNAL   Microbiol Resour Announc 7 (1), e00835-18 (2018)
   PUBMED   30533609
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Atkinson,K.V., Bishop,L.A., Rhodes,G., Salez,N., McEwan,N.R.,
            Hegarty,M.J., Robey,J., Harding,N., Wetherell,S., Lauder,R.M.,
            Pickup,R.W. and Wilkinson,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Division of Biomedical and Life Sciences,
            Lancaster University, Furness B77, Bailrigg, Lancaster, Lancashire
            LA1 4YT, UK
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/08/2024 01:00:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MH251947.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Staphylococcus aureus subsp. aureus ECT-R 2"
                     /mol_type="genomic DNA"
                     /strain="ECT-R 2"
                     /isolate="Lancaster/2015/3"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /sub_species="aureus"
                     /db_xref="taxon:889933"
                     /plasmid="pLUH01"
                     /geo_loc_name="United Kingdom: Lancaster"
                     /lat_lon="54.05 N 2.8 W"
                     /collection_date="2015-01"
                     /collected_by="K.V. Atkinson"
     gene            complement(164..487)
                     /gene="qacC"
                     /locus_tag="HUF37_RS00005"
                     /old_locus_tag="ECTR2_RS13725"
     ARG             complement(168..487)
                     /name="qacC"
                     /gene="qacC"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            809..1813
                     /locus_tag="HUF37_RS00010"
                     /old_locus_tag="ECTR2_RS13730"
     CDS             809..1813
                     /locus_tag="HUF37_RS00010"
                     /old_locus_tag="ECTR2_RS13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199882.1"
                     /translation="MQYNTTRCIDENQDNETLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVVKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENSYFKNKANYITQEEWVNLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDERNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     ARG             complement(165..487)
                     /name="qacJ"
                     /gene="qacJ"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="71.96"
                     /coverage="100.0"
     Typing          810..1813
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.801"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac ccttaactaa acttgcagac
       61 gaatgtcggc atagcgtgag ctattaagcc gaccattcga caagttttgg gwttgttaag
      121 ggttccgagg ctcaacgtca ataaagcaat tggaataaag caattaatgc gatgttccga
      181 aaatgtttaa cgaaactacg ccgactatga ttaaaactat agatactata gttattagat
      241 ttatttgttc tttgaaaata attattgaga ctacggttgt taagactaaa cctagtcccg
      301 cccaagttgc ataagttata tttagtggta ggtgttgcat tgttttactt aaaaaataga
      361 aacaaattcc aaatgaaatt attgttccta aggatggtat aaattttgaa aagccttctg
      421 aagatttaag aaatgcactt ccaataactt cagtacttat ggctattatt aaataaatat
      481 aaggcatttt taactccttt taattttcgt attttattta attttattct aagtattttt
      541 taattgtagt cttgtatttt gagtgtcatt cgcgtggtat ttttgtggat tgatggtttt
      601 tgaaaggttt ttgaaacgag ttgaaaacga gtttcttctt gtcttgatac tatatagaaa
      661 taactcgatt ttaaaattat agctgtaatc cttggtatgt aagggattat agctattttt
      721 cttgttgaaa ggaatttttg acataaaaaa accccagtag tatgattaaa gcgtctactc
      781 aataaacaac tactggaggt ctctttatat gcaatataat actactagat gtatagacga
      841 aaatcaagat aacgaaacac ttaaagatat gacgaaaagt gggaaacaac gcccatggag
      901 agaaaagaag atagataatg taagttatgc agatatactg gaaattttaa aaataaaaaa
      961 ggcttttaat gtaaaacaat gtggtaacgt cttagagttc aagccgactg atgaaggtta
     1021 tttgaagtta cataagacat ggttttgtaa gtcgaaactc tgcccagttt gtaattggag
     1081 gcgtgctatg aaaaatagtt atcaagctca aaaagtgatt gaagaagttg ttaaagaaaa
     1141 accaaaagcg cgttggttat ttttaacact ttcaacgaaa aatgcgatag atggggatac
     1201 tttagaacaa agtttgaaac atttaacgaa agcatttgat aggttaagta gatataaaaa
     1261 agtgaagcaa aatcttgttg ggtttttgcg ttcaacggaa gtaacagtta ataaaaatga
     1321 tggtagttat aatcaacata tgcatgtttt attatgtgtt gaaaatagtt attttaagaa
     1381 taaagctaat tatataactc aagaagaatg ggttaattta tggcaaaaag cattacaagt
     1441 aaattatcga cccgtagcaa atattaaagc gatcaaacca aatcaaaaag gcgataaaga
     1501 tattcaagca gctatcaaag aaacctctaa atattcggtt aagtcatctg attttttaac
     1561 tgatgatgat gaaagaaatc aagaaatcgt gaatgatttr gaaaaaggtt tatatcgaaa
     1621 acgtatgttg agttatggtg gtttgcttaa acaaaaacat aaaattttaa atttagatga
     1681 tgccgaagat ggcaatttga ttaatacaag tgacgaagat aaaacaacag acgaagaaga
     1741 aaaagcacat tcaattacgg caatttggaa ttttgaaaaa caaaattatt atttaaaaga
     1801 tttgaaacgt tagctaaaaa gctagcgttt ttttatgtct ttttgcccct gcgggaacta
     1861 tagcgttcaa ccggcatggt tgaccagttt tcatgttaac ttttaagttt gcatgtaaat
     1921 gggcagtgtc taaaaaatta gtcactggtt ttgctcaaat ttttgtttgt gaaattaaga
     1981 tatatttatt tggctcatat ttgcgtttta aaagcgtata tagactttta ggtataaaac
     2041 tatatgattt acccctgaat ctttaaaatg cccccttaaa attcaaataa aggcatataa
     2101 aaataaaaag ccaacccaga taaaatggat tggcttttta tttgcttttg cgaagtagat
     2161 tataaaaaac tacttttaat aagcacccta aatgtgatgt ctatattata acaagaacac
//
