LOCUS       NZ_MH090915             2418 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Staphylococcus petrasii strain NRL/CEM 08/435 plasmid pLF08,
            complete sequence.
ACCESSION   NZ_MH090915
VERSION     NZ_MH090915.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14228259
KEYWORDS    RefSeq.
SOURCE      Staphylococcus petrasii
  ORGANISM  Staphylococcus petrasii
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Fisarova,L., Doskar,J. and Pantucek,R.
  TITLE     Variability of resistance plasmids in coagulase-negative
            staphylococci and their interspecies horizontal transfer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Fisarova,L., Doskar,J. and Pantucek,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2018) Experimental Biology, Masaryk University,
            Kamenice 5, Brno 62500, Czech Republic
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: A5-miseq pipeline 2015
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/20/2023 02:36:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MH090915.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Staphylococcus petrasii"
                     /mol_type="genomic DNA"
                     /strain="NRL/CEM 08/435"
                     /isolation_source="blood culture"
                     /db_xref="taxon:1276936"
                     /plasmid="pLF08"
                     /geo_loc_name="Czech Republic"
                     /collection_date="2008-05"
     gene            complement(311..1315)
                     /locus_tag="HTR73_RS00005"
                     /old_locus_tag="pLF08_1"
     CDS             complement(311..1315)
                     /locus_tag="HTR73_RS00005"
                     /old_locus_tag="pLF08_1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199882.1"
                     /translation="MQYNTTRCIDENQDNETLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVVKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENSYFKNKANYITQEEWVNLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDERNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     gene            1637..>1732
                     /locus_tag="HTR73_RS00020"
                     /pseudo=""
     CDS             1637..>1732
                     /locus_tag="HTR73_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /name="SMR family transporter"
     gene            1779..2102
                     /gene="qacC"
                     /locus_tag="HTR73_RS00015"
                     /old_locus_tag="pLF08_2"
     ARG             1780..2099
                     /name="qacC"
                     /gene="qacC"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1780..2102
                     /name="qacJ"
                     /gene="qacJ"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="71.96"
                     /coverage="100.0"
     Typing          complement(312..1315)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaaagatt caggggtaaa tcatatagtt ttatacctaa aagtctatat acgcttttaa
       61 aacgcaaata tgagccaaat aaatatatct taatttcaca aacaaaaatt tgagcaaaac
      121 cagtgactaa ttttttagac actgcccagt tatatgcaaa ttaaaaattt agcaaactgg
      181 gcagaaaaaa ataaacactg cccatttaca tgcaaactta aaagttaaca tgaaaactgg
      241 tcaaccatgc cggttgaacg ctatagttcc cgcaggggca aaaagacata aaaaaacgct
      301 agctttttag ctaacgtttc aaatctttta aataataatt ttgtttttca aaattccaaa
      361 ttgccgtaat tgaatgtgct ttttcttctt cgtctgttgt tttatcttcg tcacttgtat
      421 taatcaaatt gccatcttcg gcatcatcta aatttaaaat cttatgtttt tgtttaagca
      481 aaccaccata actcaacata cgttttcgat ataaaccttt ttctaaatca ttcacgattt
      541 cttgatttct ttcatcatca tcagttaaaa aatcagatga cttaaccgaa tatttagagg
      601 tttctttgat agctgcttga atatctttat cgcctttttg atttggtttg atcgctttaa
      661 tatttgctac gggtcgataa tttacttgta atgctttttg ccataaatta acccattctt
      721 cttgagttat ataattagct ttattcttaa aataactatt ttcaacacat aataaaacat
      781 gcatatgttg attataacta ccatcatttt tattaactgt tacttccgtt gaacgcaaaa
      841 acccaacaag attttgcttc acttttttat atctacttaa cctatcaaat gctttcgtta
      901 aatgtttcaa actttgttct aaagtatccc catctatcgc atttttcgtt gaaagtgtta
      961 aaaataacca acgcgctttt ggtttttctt taacaacttc ttcaatcact ttttgagctt
     1021 gataactatt tttcatagca cgcctccaat tacaaactgg gcagagtttc gacttacaaa
     1081 accatgtctt atgtaacttc aaataacctt catcagtcgg cttgaactct aagacgttac
     1141 cacattgttt tacattaaaa gcctttttta tttttaaaat ttccagtata tctgcataac
     1201 ttacattatc tatcttcttt tctctccatg ggcgttgttt cccacttttc gtcatatctt
     1261 taagtgtttc gttatcttga ttttcgtcta tacatctagt agtattatat tgcatataaa
     1321 gagacctcca gtagttgttt attgagtaga cgctttaatc atactactgg gtttttttta
     1381 tgtcaaaaat tcctttcaac aagaaaaata gctataatcc cttacatacc aaggattaca
     1441 gctataattt taaaatcgag ttatttctat atagtatcaa gacaagaaga aactcgtttt
     1501 caactcgttt caaaaacctt tcaaaaacca tcaatccaca aaaataccac gcgaatgaca
     1561 ctcaaaatac aagactacaa ttaaaaaata cttagaataa aattaaataa aatacgaaaa
     1621 ttaaaaggag ttaaaaatgc cttatattta tttaataata gccataagta ctgaagttat
     1681 tggaagtgca tttcttaaat cttcagaagg cttttcaaaa tttataacat cctttagaat
     1741 aaaattaaat aaaatacgaa aattaaaagg agttaaaaat gccttatatt tatttaataa
     1801 tagccataag tactgaagtt attggaagtg catttcttaa atcttcagaa ggcttttcaa
     1861 aatttatacc atccttagga acaataattt catttggaat ttgtttctat tttttaagta
     1921 aaacaatgca acacctacca ctaaatataa cttatgcaac ttgggcggga ctaggtttag
     1981 tcttaacaac cgtagtctca ataattattt tcaaagaaca aataaatcta ataactatag
     2041 tatctatagt tttaatcata gtcggcgtag tttcgttaaa cattttcgga acatcgcatt
     2101 aattgcttta ttccaattgc tttattgacg ttgagcctcg gaacccttaa caatcccaaa
     2161 acttgtcgaa tggtcggctt aatagctcac gctatgccga cattcgtctg caagtttagt
     2221 taagggttct tctcaacgca caataaattt tctcggcata aatgcgtgtt cttgttataa
     2281 tatagacatc acatttaggg tgcttattaa aagtagtttt ttataatcta cttcgcaaaa
     2341 gcaaataaaa agccaatcca ttttatctgg gttggctttt tatttttata tgcctttatt
     2401 tgaattttaa gggggcat
//
