LOCUS       NZ_MG649065             5167 bp    DNA     circular CON 02-JUL-2023
DEFINITION  Escherichia coli strain Ec001 plasmid pMG2, complete sequence.
ACCESSION   NZ_MG649065
VERSION     NZ_MG649065.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14228412
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Giufre,M., Errico,G., Accogli,M., Monaco,M., Villa,L., Distasi,M.A.,
            Del Gaudio,T., Pantosti,A., Carattoli,A. and Cerquetti,M.
  TITLE     Emergence of NDM-5-producing Escherichia coli sequence type 167
            clone in Italy
  JOURNAL   Int J Antimicrob Agents 52 (1), 76-81 (2018)
   PUBMED   29501819
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Giufre',M. and Carattoli,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2017) Infectious Diseases, Istituto Superiore
            Sanita', viale Regina Elena 299, Rome, Italy
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: SPADES v. 3.8
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/02/2023 04:24:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MG649065.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec001"
                     /db_xref="taxon:562"
                     /plasmid="pMG2"
     gene            complement(1..915)
                     /locus_tag="HTQ05_RS00005"
     CDS             complement(1..915)
                     /locus_tag="HTQ05_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            1090..1503
                     /locus_tag="HTQ05_RS00010"
     CDS             1090..1503
                     /locus_tag="HTQ05_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023143259.1"
                     /translation="MYAPLKTPSVAFWRKKRKKTRKHQAKPEKRVRIVFRALHNYARKR
                     CAIGFVRSEHRTAQNDKFSPLVRGALTGFPVSKLHISPGQKKTDLGSVARIRVYGKYFS
                     YRMLNIHKRKKCLSDALKQPFQKESALYDARKR"
                     /name="hypothetical protein"
     gene            2652..2861
                     /locus_tag="HTQ05_RS00015"
     CDS             2652..2861
                     /locus_tag="HTQ05_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(2927..3460)
                     /locus_tag="HTQ05_RS00020"
     CDS             complement(2927..3460)
                     /locus_tag="HTQ05_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3463..4389)
                     /locus_tag="HTQ05_RS00025"
     CDS             complement(3463..4389)
                     /locus_tag="HTQ05_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4566..4964
                     /locus_tag="HTQ05_RS00030"
     CDS             4566..4964
                     /locus_tag="HTQ05_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(141..915)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3467..4389)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctactctacg agacctcgtt ttttcagttg gtaataccag gcgcgactga tccctaatgc
       61 ctcccacggt ttcagcgtgc gtgcagatgt agctactgtg ctgcgcttag acttagaacc
      121 gcccttgcga ccgcgtgcag cctgaatttc tggcgtgtgc gtatcagcca catactgtgc
      181 gaaagtttcc ggcgtgaagt tcctgtgcgt gtatttcgca atgctcttgc caatagcccg
      241 acattcagcc agtgaaagcg gaacgggaag cgatgcattg tacatttcga cacgctggat
      301 caccgcttca agccactgtg agaacgctgg ccagccctga cgaatcgccc tgtaagccca
      361 tttgcgcgtc atttcgaaca ggtggcagtt tcgccccatc ccgtaatgtt tatcgatgct
      421 acgacgctct gaggcgctca aatcgagata atcagccagt tcatcgaggg tataggcttc
      481 ctcgcgccac tccatcacca gccattccag gtggaacgga tttttgcaaa tcaggccgct
      541 gtagttcaca tccgcacaaa gtttctcaca cagcgaacgt tcaaccgctg cggcatattt
      601 cagcgcctta accgaggcat caggcgcggt tctcacggcg atgttgagtg cgtagagcag
      661 gtgagcgtgt ccgttcacag gatttttaac ggtgatgttc ggcgcgggag cgttccggtc
      721 gctccagtca atcgcagccc cctcgcggtc aacgtcaaaa accagccaga actgcgcatg
      781 aggctggttc tgctgtatgt accgcgcaag aagcgcacgc cctttgccgg agatgcgaag
      841 accaaaagcg agatcatccg tgtgataggg acgatggggt aaattttcgt cgaagtattg
      901 aagcgcggcg ctcatgtgtt acgattctct ccgggttatg cgacctacac gccctctgta
      961 tttcatgttg aatcttgccg gatctggaca tgatttcagg ggcgtttgtt tttcagtcgc
     1021 ttacactaca cccaacggca aatcctgtcg aaccttcccg aaaacccgat ccatttacca
     1081 taactcccgt tgtacgcccc ccttaaaacg ccctcagtcg ctttctggcg caaaaaacgc
     1141 aaaaagacaa gaaaacacca ggccaaacca gaaaaacgcg tcagaatcgt tttcagggcg
     1201 ttgcataact atgcacgaaa gcgatgtgcg atcggtttcg tgcgttctga acaccgaacg
     1261 gctcaaaacg acaagttttc tcccctggtg cgtggcgctc tgacggggtt tcctgtttcc
     1321 aaacttcaca tctcgccagg acaaaaaaaa accgacctgg ggtcggtggc aagaatcaga
     1381 gtctatggga aatattttag ttatcggatg ctcaatattc acaaacgcaa aaaatgcctc
     1441 agtgatgccc taaaacaacc tttccagaag gagtccgcgc tttacgatgc acggaagaga
     1501 taaaaaaccc cacaaaagtg gggaaccaag agaaacaaca attacaggtt gttggttgtg
     1561 ttcgaacaac gatgaagtag ctacgctaaa taataattac cgtacaggcc ctacatattc
     1621 aacactaaat ttaagatcct gacgaaggat ttataaatgc acgaaaacac atagacagac
     1681 aaattcacta aattcataca caaaaacaag tgtagttttc taagtaaatt agattcgaca
     1741 accaattagt ttatccacat cgcattccac tcttgctaca ccaatcacat ttcttgatat
     1801 gtaatagcag aaaaaataaa accagacacc aatagaccaa aaaacaaaca ttcaacaaga
     1861 aacattttga acaatatcac aaaactgtca caaccatcat cagactattg aaaaaaactg
     1921 gttgctgttt tataaaagcg actgtaacaa cctccactct atagttccat tagtttaaag
     1981 tgtcacacaa actgagcatc catttgtttt ccttactttt gcccctgtat tccaaggggt
     2041 ttttttataa cttaaagatt agcacaccac acaaaaacaa taaaaaaaaa cgacaagtta
     2101 tacaataaaa ccagtactat ccatacagct aattacatat aaatactatt aaaccatctg
     2161 gacattaaaa ataaactctt ataacataaa attaatgcgg ttccggtacc atatgtcagg
     2221 gataatgtgc aaatggaggt atcggaactg catttcagtt cattattaat taatgacaaa
     2281 aaaaacctct gcgagcgcag agggggtaag gaatgttccc ggtcataata tgtcgatagt
     2341 gtttatatac atacagaaca agaacaacaa ccagccaact aataaatatt ttatttttta
     2401 gagctgaaac cttaaagctc acttccattc catacattgt tataaacctt atgttgaaaa
     2461 ctgaaagtaa gtacggcaga cttattaagt tgcgtgcagt cttttcacgt tatacaaaca
     2521 agaacacctg cgaagttatt tctccttaca tatcccccta tgccaacggg ggattttttc
     2581 tggttcacta acgcataaat aatagtgaca ccctatcatt cacaaaccgt gataatataa
     2641 ggattatcat catggcaaaa ttaggtgaaa acaccccaca tattatcgat gctactgtag
     2701 attttatggc atcaagccag gcatttcgtg agtatctgaa aaaacttccc cctgataaca
     2761 caataccgcc agctcttact gatgagcacg taccgttata tatacgaagg ctggaatact
     2821 atcgtctgct ttatcgacca aagcagacag aatcatattg aaaaaaacgg tgaacggttc
     2881 gcgaaattct ttcgcgggtc gtgaggcgtt ggacaagcga agcgcgtcag tttttccggc
     2941 tcttccccgt gacaggtttc tccaccagtt tatcttccag tgtcacaact ttctgataca
     3001 ccgccgctgt ggcgttgtag ttcctgtcga gtattgtttt cattccccat ccgagcacac
     3061 agaacataac cagaaaagcc actgacaccc cacccagcca tttccagcta taccctttaa
     3121 gggtgttgta ttcctgcacg atattgctgt gaattgtcgc aatatccctg ttcagggcat
     3181 ccgcgtttgc cttcatgttt gcgactgact cttccagccg ctgaaaatcc tggctgatgc
     3241 gctcacctat cagtcctgcc acgtccagct tctgcacctc atttctgact tcatccctga
     3301 cctgttttgc cacaccgtca gaaatatccc gatatccgcg aagagtttcc cttgccacag
     3361 ccagagaatc agcaacagct ttctgttgct ccattgcggt atgttgcaga tgctccaccg
     3421 ataacaggag cgtaagtaat ttttcttctg tgctggccat gttcagaatc ccatagaaaa
     3481 tccacgtgac ggtgatttaa cccgttcagg tgcagttcgg ttctgtctgc tcactctgtc
     3541 tctcatgtca aagccatgct cccgcgcaac atcatgttgc agccttctga ccagttcatg
     3601 ttgcttaacc ggagtgtcca gccattccct gatgtcatgc ccgaccgttc gcgcctgttc
     3661 aagatggcgg gaatagtccg gttcagaccg tttttgcatg atgtcatgac gctgggcatc
     3721 atcaagccgt ccggcagctt caaaacccag atgcacttcg ggacgtcgtt caatgccctg
     3781 ttctttcagc gtccggcagt caatgaagcc cctttcaaag ccatgctttc tcatatgttc
     3841 gttgtgcttc acctcccagc gttttcgcaa ttcccgcaac tgctcttttt gctgagtgag
     3901 agtcaggctt ccggaatcct ttttcgcacc tccccgctcg ggatacctgg cgttatagcg
     3961 ccggaaatac tgttccggtg tacgttcaat gccatcattc acacgctgac tcatcatgat
     4021 atgcgcatga ggctgttcgc cgccgtcaat gctcgctttc ggattgtgaa tggcaaaact
     4081 ccaggcgtgc cgttcgccag cttcctgacg tacaaaatcc tgcaccagtt caaggcgctg
     4141 ttccggtgtc agttcacggg gtaatgcaat ttcaagttcc ctataggtcg aaccgttcgc
     4201 acgttcaaac tggtcagccg cctgccagaa atgggaagga ttatccctgg cccattccgg
     4261 catgtttccg tgtgcggaat attccaaatc ctgacggtcg cggtatttct ctttgcgctc
     4321 gatgtaatcg gcgtggtttg cagcctgccc cttgccacca aatttaacgc tgaggtgata
     4381 ggttgccacg gtttcagccc tctgcaggag cgcactgttt ctcgacgaag gagagaaaca
     4441 tataagtgcg cttccttcta attttacgaa ttagcacgga agataccccc tgcacccgcc
     4501 gaccgcaagc gatatactgc acgaccacaa cacaccgcaa aaaaacacca gggagcatca
     4561 cgaacatgcg catcagcaac atcgaatggc tgaagaaacg gatcggattc atcaggaagc
     4621 tcggagaaca gaccgcacga cagagacaga ttattgacct gatcgacaac gaagccggac
     4681 tcacagaaca ggaacgcaaa cttctccacg tactggcaac agcggaaaaa aacgacttac
     4741 aggcacagga gagcgaacga aaacaggcgg tccaaaaacg aatcgaggga aaaaaacaac
     4801 gacgggaacg caatcaccgg ctctttctcg cggctgggct actgatcgaa gcaggactgg
     4861 tagacacaaa aacaggcgag ctatgctata aaaaagacag gatattacaa gcacttaagg
     4921 aacttaagta cgatctggag acatccccaa acccagacgc gtaagtgcct ccatccgtgt
     4981 tccgattccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg agactgcggc
     5041 gagcactacc accacaatca cgcggaaagc cgcgccattg cagggctgaa cggggaaaaa
     5101 cggcaaaaaa cctgctgctc aaaaagacgc cagaaaggcg ctgttatctg atataggctt
     5161 atttggt
//
