LOCUS       NZ_MG649064             4773 bp    DNA     circular CON 25-APR-2024
DEFINITION  Escherichia coli strain Ec001 plasmid pMG1_1, complete sequence.
ACCESSION   NZ_MG649064
VERSION     NZ_MG649064.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14228413
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Giufre,M., Errico,G., Accogli,M., Monaco,M., Villa,L., Distasi,M.A.,
            Del Gaudio,T., Pantosti,A., Carattoli,A. and Cerquetti,M.
  TITLE     Emergence of NDM-5-producing Escherichia coli sequence type 167
            clone in Italy
  JOURNAL   Int J Antimicrob Agents 52 (1), 76-81 (2018)
   PUBMED   29501819
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Giufre',M. and Carattoli,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2017) Infectious Diseases, Istituto Superiore
            Sanita', viale Regina Elena 299, Rome, Italy
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: SPADES v. 3.8
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009979715.1-RS_2024_04_25
            Annotation Date                   :: 04/25/2024 03:12:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MG649064.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec001"
                     /db_xref="taxon:562"
                     /plasmid="pMG1_1"
     gene            459..782
                     /locus_tag="HTP97_RS00005"
     CDS             459..782
                     /locus_tag="HTP97_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957080.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLAEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            991..>1425
                     /locus_tag="HTP97_RS00035"
                     /pseudo=""
     CDS             991..>1425
                     /locus_tag="HTP97_RS00035"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1460..1945
                     /locus_tag="HTP97_RS00015"
     CDS             1460..1945
                     /locus_tag="HTP97_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_079967943.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKVAFSEHEKFVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTIIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1949..2164
                     /locus_tag="HTP97_RS00020"
     CDS             1949..2164
                     /locus_tag="HTP97_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137420.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMFGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2363..2914)
                     /locus_tag="HTP97_RS00025"
     CDS             complement(2363..2914)
                     /locus_tag="HTP97_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654246.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(3483..3770)
                     /locus_tag="HTP97_RS00030"
     CDS             complement(3483..3770)
                     /locus_tag="HTP97_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000493377.1"
                     /translation="MFTACTVELAIYWYATTNNIPDKTSHIINGRISQLNQFLLVLSPD
                     MANTKAPVRASMPTNTQEDVASRNNITFRINFRDTAIAIKPIRVSYSAVR"
                     /name="hypothetical protein"
     gene            complement(4511..4594)
                     /locus_tag="HTP97_RS00040"
     CDS             complement(4511..4594)
                     /locus_tag="HTP97_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058652899.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPDRHD"
                     /name="hypothetical protein"
     Typing          171..355
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.17"
                     /coverage="86.0"
                     /evalue="5.83e-49"
     Typing          complement(3..225)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.625"
                     /coverage="99.0"
                     /evalue="1.87e-77"
     Typing          complement(4295..4773)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.454"
                     /coverage="94.0"
                     /evalue="8.02e-146"
     Typing          773..2268
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.385"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
       61 gcagcgccgc atccttttcc tgtcctgctt gccatgccgt atgcctttgc tcttatccgt
      121 ttgcaggtaa cgcccgccgc agccgaacgt caggagcgga agcgaccgag tgagcgagga
      181 ggcgtcatat caccgtaacc tccattttct cctgtacgcc ctgcagctgc atttttcccc
      241 tgccacatga agcactttca ttacgtgtta ctccgccgta acatattgag ccagtataca
      301 ctccgctagc gctacgtgac tggttcaggg ctgcgccccg aaacctgcta aaaccactga
      361 cgcgcctgcg gcttgtccaa caccgcgccg accgggaaaa gatttcccgc ccgtcccggc
      421 gttatcagga gatcggtttg tcagagaaac ggaacaagat gctcaccatg tgggtgaccg
      481 aagatgagca ccgccgtctg ctggaacgct gcgacggtaa acagcttgca gcctggatgc
      541 gccagacgtg cctggccgag aaacccgccc gcgccggcaa gcttccctca atctcgccgg
      601 cgctgcttcg tcagcttgcc ggcatgggga ataacctcaa ccagattgcc cggcaggtta
      661 acgccggtgg cggcagcggg cacgaccgcg tgcaggtcgt cgccgcgctg atggccatag
      721 atgccggact cgagcggctg cggcatgccg tgctggaaaa gggtgcagac gatgatcgtt
      781 aagtttcacc cgcgagggcg cggcggtggc gccggtccgg tggattatct gctggggaaa
      841 gaccgacagc gcgacggcgc caccgttctg caggggaagc cggaagaagt cagggagctt
      901 atcgacgcct cgccctacgc aaaaaagtac acatccggcg tcctgtcctt tgccgaaaag
      961 gatttaccgc ccgggcagcg cgaaaagctg atggcgagct ttgaacgggt tctgatgccc
     1021 ggactcgata aagaccagta cagcgtgcta tgggttgagc accaggataa ggggcggctg
     1081 gagctgaact tcctgatccc gaacatggag ctgctgacag gcaggcgtct ccagccgtat
     1141 tacgaccgcg ccgaccgtcc gcgcattgat gcctggcaga ccatcgtgaa cggcaggctg
     1201 gggctgcatg acccgaacgc gcctgaaaac cggcgggcgc tggtcacgcc gtccgcgctg
     1261 ccgaaaacga agcaggaagc cgctcaggca attacacggg gtttactggc cttagcctcg
     1321 tccggggagc tgaaaacccg ccaggacgtc actgaggcgc tagaaagcgc aggttttgag
     1381 gtggtgcgca ccacaaaaag cagcatcagc attgccgacc cggacggggg gcgaaacatc
     1441 cgactgaagg gagccatcta tgaacagtct tttaacgctg gcgaaggact tagagcagaa
     1501 atcgaaagcg cagcagcaga gtaccggcga gatgctgaaa gtcgcattca gcgagcacga
     1561 gaagtttgtc agagcggaac tgagcgaaag cgagaagaga atcagcgccg ccatcctcga
     1621 ccacgaccgg aagctgtcct cagccatgag ccagcgcacg aaaggcatgg tgcgtatggt
     1681 cagccagacg tggctgacca tcgtcctggt gtctacactg ctgacagcat cgggcgcaag
     1741 cattctatgg tggcaggggc agcagatact cgacaattac acgatcatcc gggagcagaa
     1801 gagcacgcag gccatgctgt ccgagaggaa cagcggcgtg cagctctcga cctgcggaga
     1861 gcagagacgg cgctgcgtga gggtgaaccc ggaagcggga cggttcggag aggattcgag
     1921 ctggatgata ctggcgggga aatagcacat gacggagctg gaaaaacagt tgctgagcgc
     1981 attagagcag ctacagcagg actactcgaa aaggctggac gagtgggaga acgccttcgc
     2041 ggaatggcgg acgatgtttg gtcttatgca acgggagaac gcggcgctga gcgagcgcgt
     2101 cacgggcttg agcaggcagg tgcagagttt gagcgagcag ctgcgccggt tgtcacaagg
     2161 ctgaacgtga tagaggcaca tcgtgagcag gaacgggcgg cgcagcatca gaaagcgctg
     2221 gagctggaac gatctcagcg acagcgaacg tacaacggtc cgtcactttg aatctgtgcg
     2281 ccggaaaagc atgggcttgc tgtttctggc gcacaggttc agttctagaa catgaggctg
     2341 ctatcttgtt gttcacgctt catcagatct tgatgcttat tgttatggct gagctatcaa
     2401 tccctgttgc gttgctaata tcctttttgg ctctttcaat tgcctcgtta agggagagtt
     2461 tttctgtttt ctcttcagcc agaccaggaa tcactgtatt aggaaccgat ggggatgaag
     2521 gatggaattt cttccattcc agttgttccg gtgttacccc aatacgtaaa aaagcttcct
     2581 gtgcgctcag gtaagcaacc ggatagcgct gaccatcggt tagcattttc catgcttttg
     2641 gagtgtgaat ttctgcgtat tcatcaaagg ttatgacgtt cctgtcgtcg taatcagggc
     2701 ttttaaaaca cccggtaatt ttggcgatca ggaatgctgt gccgtgttcc aggtctttta
     2761 ctgcccacgt ttcccttctg tttcttactg ctatgaggta atcggcatcg ttgagcctgt
     2821 ctgttctggc atgccagtag cctgaccccc cctcggttct cattatgttc aaatccttcc
     2881 cggtaaaaac cacaacagct ttcatatctt tcatatagaa acccttaata ggtgagtcaa
     2941 tttcattttt ttatgaaatc tatacgatag aattcgtgtt gtaaatgttt tttatgcgat
     3001 aaaatgcagc cccctacctc ttttattgtt agtgataagg cttcctgttt gtttttttga
     3061 taaaccggtt aaaacagggg cttggacgag gatatacatt gaaagcatga gccattcacg
     3121 tggctgcatg gctatgcagc cgcgtgaaat ttatcaggcg cagaatgcgc ggtttagcgg
     3181 ggagtttact gccggtttta ccgggctaac gccgctttca cgctgaaccc gtccggagcg
     3241 gtgcgggcaa gggatgttat ggtaaatcta gctgctaggg cgattcgcac gtcacatttt
     3301 caggtgtgat atccttatgc gccaaggatg aaaggacaaa aaaataatcc ataaggattc
     3361 tttttatcgt ctctgctttt ttcttgggct ccttccgctt gtttcctctc tggtaccata
     3421 tgcggactct actcaataag gatcgttttg tgcctgctcc aaacaattac ggtgtagctg
     3481 gttcacctaa cagcgctata gctcaccctg atgggcttga ttgctatggc cgtgtccctg
     3541 aaattaattc taaaggtgat gttattcctg ctggcgacat cttcttgcgt gttggtaggc
     3601 atactggccc taaccggggc tttggtgttc gccatatctg ggctgagcac gagcaggaac
     3661 tggttaagct gggatatacg accgttaatg atgtggctcg ttttgtccgg gatattattc
     3721 gtcgtggcgt accaatatat tgcgagttca acagtacaag cggtaaacat cggcctgcag
     3781 tgctaaagtc gtcggtaggc atcgtaattc ttgagcccag agaagcgcct gaaactgagt
     3841 caggttggat ctatgtcgtt gtgactgcgt acacacgaag gacggcgcac ggtactttgg
     3901 ttggtcatat tgaataaaaa aatagggcgc taaaaagcgc cctttatgaa ttcgttggta
     3961 gtcgcagcat ttccttagca catagggttc agaaaccgac gcttgtcgta actacccggc
     4021 agatgaccag ccatctgact gcccggcaca gaaggcctat tactggttga tgccccctgc
     4081 gaatgctctt gcagtacaat aatcataaac atcgtgatgt agatttgcaa ccaacatttt
     4141 ggttgcaacc tatatactaa tttttttcga taaccctgga atccattgct gtgtctggat
     4201 ttcttgaagc tcctggctat agatacccta tatcctaaca gttattctgc tgtgaatatc
     4261 agaaatgaac acttgagatc atcagtggct cgtcgtagtc tcttgctcag aaacgaaaaa
     4321 accaccctgg caggtggttt tttcgaaggt tagttaatcc tggcagattc tctaaccgtg
     4381 gtaacagtct tgtgcgagac atgtcaccaa atactgtcct ttcagtgtag cctcagttag
     4441 gccgccactt caagaactct cgttacatct ctcgcacatc ctgcttacca gtggccgttg
     4501 ccaatggcga ttagtcgtgt ctatccgggt tggactcaag acgatagtta ccggacaagg
     4561 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     4621 acccggaact gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaatgaca
     4681 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc
     4741 tggtatcttt aagtcctgtc gggtttcgcc acc
//
