LOCUS       NZ_MF807213             5143 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Streptococcus thermophilus strain CS17 plasmid pCS17-B, complete
            sequence.
ACCESSION   NZ_MF807213
VERSION     NZ_MF807213.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227592
KEYWORDS    RefSeq.
SOURCE      Streptococcus thermophilus
  ORGANISM  Streptococcus thermophilus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Hu,T. and Cui,Y.
  TITLE     Isolation and sequence analysis of three native plasmids from
            Streptococcus thermophilus CS17
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Hu,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2017) Department of Food Science and Engineering,
            School of Chemistry and Chemical Engineering, Harbin Institute of
            Technology, No. 92, Xidazhi Street, Nangang District, Harbin,
            Heilongjiang 150001, China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology        :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/20/2023 02:36:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MF807213.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Streptococcus thermophilus"
                     /mol_type="genomic DNA"
                     /strain="CS17"
                     /db_xref="taxon:1308"
                     /plasmid="pCS17-B"
     gene            complement(696..992)
                     /locus_tag="HTO59_RS00005"
     CDS             complement(696..992)
                     /locus_tag="HTO59_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360723.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(1119..1841)
                     /locus_tag="HTO59_RS00010"
     CDS             complement(1119..1841)
                     /locus_tag="HTO59_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002360724.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            2488..2574
                     /locus_tag="HTO59_RS00015"
     CDS             2488..2574
                     /locus_tag="HTO59_RS00015"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2759..3070)
                     /locus_tag="HTO59_RS00020"
     CDS             complement(2759..3070)
                     /locus_tag="HTO59_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360725.1"
                     /translation="MKKYLIKNIFYITIPLVLSYITSFLVNIDLPILIIIFYGILLFFL
                     IPSEVYLGSTMDYNAKVVNPTYRPENKSFEDSPKSKILSILIVLLCLILTISIWYFSN"
                     /name="hypothetical protein"
     gene            complement(3093..3293)
                     /locus_tag="HTO59_RS00025"
     CDS             complement(3093..3293)
                     /locus_tag="HTO59_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(3382..5142)
                     /gene="mobV"
                     /locus_tag="HTO59_RS00030"
     CDS             complement(3382..5142)
                     /gene="mobV"
                     /locus_tag="HTO59_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360721.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_002360721.1"
                     /translation="MRYTLLGSTAEIQRHRTTQEKKGGGALMSKIVARMEKMKDGNLSG
                     IQRHNQRETNNHSNPDIDIEKSHLNYDLVNPGSINYREKIKQIIESQRISKRAVRKDAV
                     LVNEWIITSDTAFFQENTDTQAFFTDVVAYFSDRCGRQNVAYATVHLDETTPHMHLGIV
                     PMYEGRLSSKQVFSRQNLLEIQEELPTYLQERGYAIERGLRGSPQKHLSVKEYKDVQKE
                     LQVSKQELANQEQILAEKERELHSYEQAIFRIDRLLEEKNEEVKGKVQAMNQLETKRNE
                     IINSLQTTVLPDLANVKEKKFSLNGLKYVLSASDFSAIKSITKDFETLRTQNKHLKIEN
                     NNLAQKNKELTQVNHEQELQITELAYELTLKKEQLTAFEEKQLNYDQSEQIKQELDTTT
                     QTVSVLETENKRLKDKITDLTYDFSELNEKYQEIKLKFDGLTTYLHERLGHAKETILFT
                     IQQGVKYLQNAPKLKKSLERYSKDRTYLIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKA
                     PFYNRETGCYEFVGEYMANGRSRTNTLQLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSR
                     DMSKGRGLSR"
                     /name="mobV"
     Typing          205..464
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="81.0"
                     /evalue="1.95e-45"
     Typing          complement(1120..1841)
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3386..5136)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atactttact tcgtaaaggt gcgctctgcc gagggctgtc ggctagtact cgaaaggtca
       61 gcctttaggc ttcccaatct cgcacttagc cgattgccta cggtcaaact ttccatttct
      121 gctaaggcag aaactccaaa tttccctccg tcagaaaacc gtttaaataa cgactgcctg
      181 aaattagtga acgtgctagc tttctgctag ccggtgctag taatgtgcta atttggtgct
      241 aaaaacctgc tagtcactgc taggaatctg ctagtttggg tgctagaaac ctgctattga
      301 gtgctaaaaa tgtgctagtt tacctgaaag tacctgctag taaagtgcta gcgaagaacg
      361 aaagtgtacc ccacgggaaa gccttgcact gcaaggcggg gtaccttgtg tgccccccag
      421 ttccccttat gctcttccga aacacaggaa gaaattaagc gaatttcaac agtaaaagca
      481 aaaaataaca gcagtgggat tgggggaacc ccaaaaattt gaacgactcc taccagtatt
      541 tttggtagga gtcgttcaaa atatcgcaac atggtaccat gttgctccgc ttgcaaaaag
      601 aaagcctacc ccaaaatgga agtaggctaa tcttgatatt tttcgagtgt tatcccaaat
      661 agtcaacatt tttttataac aagcaagggt agtttttatt cttcttcgtc tagagattga
      721 ccgtcaatct tcacaatccc acaggaaata tcttcttcta gcgtaacttt gtaggctacc
      781 gtatcaataa gtagaatgta tgaccgactt agataattaa acattcttag cgtttcgtct
      841 cgaactacag tatctaatcg tgataagtca agtggctcca aatcaagcaa tagcgcttct
      901 agttcactct caaacttatc tagtcgctcc agtacagcct gtgtgacttc gtactcctct
      961 tttgtcatgt tgtatcctgt cattaatccc ataagtagga cctccaaaat aatttatttt
     1021 agaagtcctc acgcaaatat gctaaaataa tctagcagtc cggaagattg cgtgaggaac
     1081 ttgctaactt gcttaatgtg agggggcaag ttcttttttt attctccgtt tacccaatcg
     1141 tgcatggtta cgcttggttt gttggttttc agaccagtaa acgtaaattc tagtttggca
     1201 atcttattgc cttttttggc tttgattttt gttacttcta aattctcaaa atagttatgg
     1261 agttctttca ttgctggttt taaaaccttt tggtcaatgt tacccatttg ataatattcg
     1321 ggtatgtcta acaattctct gaagtcttca atactgacta cataataccc tgttgaacga
     1381 tattgcatta acaaacgaaa caatgtttta gcatatgttg agcgtattga agtaaaagcg
     1441 gacagctcaa acctactaaa ctctgttgtt aaaccattta ttatatagtc taaatcttga
     1501 ttaactcgaa cttcaacaaa cttttgtttt ttgtcgattt taaatccatt gaataaaaca
     1561 aaatactcta tttcgtcatc gttttctgta cgataagaaa ggttcagcat atctttatac
     1621 aacttttcta agtctgctac aaaggctttt gtagcagtca ttttgtagtc actcaattcc
     1681 tttaattcct cgaaggagaa acgaatattc tctactcctt tatctttcat tcgtgcgcat
     1741 aacgcaaaga acagatccat ttgttttgca ttaaagtttt taagtggcac aagatttaac
     1801 tcattttgat atttaacagc taattccgat ttatcactca ttcctttcgc cccctcacaa
     1861 ttcgaatatt aaaacaaaca accaactttg tcaacagttg gttgtttgtt ttaatattat
     1921 gaaaataaaa cagataaaaa gttgttggta gtcagataaa aagttgttgg tagtcagata
     1981 aaaagttgtt ggtagtcaga taaaaagttg ttggtagtca gataaaaagt tgttggtacc
     2041 atctgctctt ccttagagcc acgtggcaca cagcccgcct aaaaaggttt taaaaatata
     2101 tataaaatat atataaaaga aacgcgtgcg cgcgcggaaa tttcccgccc aaaattaacc
     2161 tcttttttct acaaacaggt ccgcgatatt tacaaaactg gcactaaaaa atcaaaaaag
     2221 gagtgagcga aaatcccgct cacattttgc ttgcagcgtt cacttcgttc actcgctaaa
     2281 atcaaaatca ataccgttga caaggtaaat catttggtta ggctctattt gcttgtctga
     2341 agatctttaa gacatgttta gttgttttag ggtatcttgg ttaggattgc gccttgcagg
     2401 cggtcagggc aagccctaac aacccccaca agaaattctt cttgtgatat acttgttttg
     2461 gcaactgccc cgaaaggggg tgtaaacatg attgaaagct taatttcgtt gattatcgcc
     2521 ccactttttg tgggtttagt acttttgcta gttgatcgtt ggctagacga ttagcagata
     2581 atctatgcag ttgcttttct agctccaacg ctgaaatcgc aacagtgtaa aaaagccctc
     2641 ctactgagaa taggagggct ttttggtgta aacatgattt tgcttaattt cgcttcgcac
     2701 ttacacaata acattttttt tctgtatttt caaacatcag attagattac gcaaaacgtc
     2761 aattagagaa ataccatatc gaaatagtta gtattaaaca tagtaatact atgaggatag
     2821 acaatatttt actttttgga ctatcttcaa agcttttatt ttctggtctg taagtgggat
     2881 tcactacttt cgcgttgtaa tccattgtac ttcccaaata tacttctgaa ggaattaaaa
     2941 aaaataataa aattccgtaa aaaataatta tcaatatggg caaatcaata ttaactaaaa
     3001 aagaagttat atagcttaaa actaaaggaa tagtaatata aaatatattt tttattagat
     3061 attttttcat gaaataacct caaatttagc aactattttg tccatcttgt atgcattggg
     3121 atacaaaatc caccggcttt atgatatcct cctgcagtcc acccgccttt tttccaataa
     3181 aatcctggct gacactctat agaatcccac tctctagcac ttgcttctgt agttgaactt
     3241 aaagcaacag agataaaaga cacccctaaa attagaacta acaacttttt cataatgctt
     3301 acctcacttt tttttaattt ttattaatat attttaattt aactgttttt atgtgtcaat
     3361 aatttatcaa ttaaattatt tttaacgaga taaacctcgt ccttttgaca tatctcttga
     3421 atatttttta ctctgttgcc aagccttttt atctgtcgac tgttcaaacg taaaagcttt
     3481 cttctctagc acttgttctt cagacagttg taaagtattc gttcgagaac gtccattagc
     3541 catatattcg ccgacaaact cataacaccc tgtttctctg ttgtaaaacg gggcttttag
     3601 ggggtagaaa tgtgggttta aggacattgt tacccctttt gaccccgtat ctggtggtaa
     3661 aaacaatgtc cctttttcgt caatgaggta agttctatcc ttggaatatc gctctaggga
     3721 tttttttaat ttaggcgcgt tctgaaggta cttcactcct tgctgaatcg taaataaaat
     3781 ggtttcttta gcatgaccta gtctttcgtg aaggtaagta gttaacccgt caaactttaa
     3841 ttttatttct tgatattttt catttagttc agaaaagtcg taggttaagt cagtaatttt
     3901 atcttttaac cgcttattct ctgtttctaa cacagaaact gtttgtgttg ttgtatcaag
     3961 ttcttgtttg atttgttcgc tctggtcata gtttagttgt ttttcttcaa acgctgttaa
     4021 ttgttctttt ttcagggtta attcataggc taattccgtt atttgtaatt cttgttcatg
     4081 atttacttgt gtcaattctt tatttttctg tgccaagtta ttgttttcga ttttaaggtg
     4141 cttattttgc gttctaagcg tctcgaaatc tttcgtaata ctttttatcg ccgaaaagtc
     4201 agaagcgctt aaaacgtatt ttaaaccgtt taaactaaat ttcttttctt tgacatttgc
     4261 taaatcaggt aaaacggttg tttgtagaga gttaatgatt tcgtttcgct tcgtttccag
     4321 ttggttcatt gcttggactt tgccctttac ttcttcgttc ttctcttcaa gaaggcggtc
     4381 aatacggaag attgcctgtt cataagaatg gagttctctc tctttttctg ccagaatttg
     4441 ttcctgatta gcgagttcct gcttagaaac ttgtagctcc ttttgaacgt ctttgtattc
     4501 tttgacagat aaatgctttt gcggactccc acggagtcca cgctcaatag catagcctcg
     4561 ttcttgcaaa taggttggca attcttcttg aatctctaac aaattttgcc gactaaacac
     4621 ctgtttactg ctcaatcgcc cttcgtacat aggcacaatt cctaagtgca tatggggcgt
     4681 ggtttcgtct aaatgtaccg tggcataggc gacattttgt cgaccgcagc gatcagagaa
     4741 atatgcgaca acatcggtaa aaaatgcttg tgtgtctgta ttctcttgaa aaaaggcggt
     4801 atcactagtg attatccatt cgttcacaag gactgcgtct tttctaaccg ctcgtttact
     4861 gattcgttgg ctctcaatga tttgtttgat tttctcccga taattgattg aaccaggatt
     4921 gactaagtca taattcaagt gagatttctc aatatcaata tcaggattgg aatgattatt
     4981 ggtttctcgt tgattatgtc gttggatacc acttaaattt ccgtctttca ttttttccat
     5041 acgtgcaaca atttttgaca tgagcgctcc tccccctttt ttttcttgtg tcgttcggtg
     5101 tctttggatt tcggcagtac ttccaagtaa agtataacgc act
//
