LOCUS       NZ_MF445422             4439 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Staphylococcus saprophyticus strain KM1053 plasmid pSSTET1, complete
            sequence.
ACCESSION   NZ_MF445422
VERSION     NZ_MF445422.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227649
KEYWORDS    RefSeq.
SOURCE      Staphylococcus saprophyticus
  ORGANISM  Staphylococcus saprophyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Lee,J.-H.
  TITLE     Characterization of Mobile Staphylococcus saprophyticus Plasmids
            Isolated from Fermented Seafood That Confer Tetracycline Resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Lee,J.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2017) Department of Food and Nutrition, Dongduk
            Woman's University, 60 Hwarang-ro 13-gil, Seongbuk-gu, Seoul 02748,
            Republic of Korea
COMMENT     ##Assembly-Data-START##
            Sequencing Technology        :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/20/2023 02:33:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MF445422.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus saprophyticus"
                     /mol_type="genomic DNA"
                     /strain="KM1053"
                     /isolation_source="Myeolchi-jeotgal"
                     /db_xref="taxon:29385"
                     /plasmid="pSSTET1"
                     /geo_loc_name="South Korea"
     gene            55..1434
                     /gene="tet(K)"
                     /locus_tag="HTO16_RS00005"
     ARG             56..1431
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1620..2861
                     /gene="mobV"
                     /locus_tag="HTO16_RS00010"
     CDS             1620..2861
                     /gene="mobV"
                     /locus_tag="HTO16_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            3388..4332
                     /locus_tag="HTO16_RS00015"
     CDS             3388..4332
                     /locus_tag="HTO16_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277652.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKKERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          3389..4332
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1621..2858
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgaaagaata atttacccct ataaacttta gtcacctcaa gtaaagaggt aaaattgttt
       61 agtttatata aaaaatttaa aggtttgttt tatagcgttt tattttggct ttgtattctt
      121 tcatttttta gtgtattaaa tgaaatggtt ttaaatgttt ctttacctga tattgcaaat
      181 cattttaata ctactcctgg aattacaaac tgggtaaaca ctgcatatat gttaactttt
      241 tcgataggaa cagcagtata tggaaaatta tctgattata taaatataaa aaaattgtta
      301 attattggta ttagtttgag ctgtcttggt tcattgattg cttttattgg tcacaatcac
      361 ttttttattt tgatttttgg taggttagta caaggagtag gatctgctgc attcccttca
      421 ctgattatgg tggttgtagc tagaaatatt acaagaaaaa aacaaggcaa agcctttggt
      481 tttataggat caattgtagc tttaggtgaa gggttaggtc cttcaatagg gggaataata
      541 gcacattata ttcattggtc ttacctactt atacttccta tgattacaat agtaactata
      601 ccttttctta ttaaagtaat ggtacctggt aaatcaacaa aaaatacatt agatatcgta
      661 ggtattgttt taatgtctat aagtattata tgttttatgt tatttacgac aaattataat
      721 tggacttttt taatactctt cacaatcttt tttgtgattt ttattaaaca tatttcaaga
      781 gtttctaacc cttttattaa tcctaaacta gggaaaaaca ttccgtttat gcttggtttg
      841 ttttctggtg ggctaatatt ttctatagta gctggtttta tatcaatggt gccttatatg
      901 atgaaaacta tttatcatgt aaatgtagcg acaataggta atagtgttat ttttcctgga
      961 accatgagtg ttattgtttt tggttatttt ggtggttttt tagtggatag aaaaggatca
     1021 ttatttgttt ttattttagg atcattgtct atctctataa gttttttaac tattgcattt
     1081 tttgttgagt ttagtatgtg gttgactact tttatgttta tatttgttat gggcggatta
     1141 tcttttacta aaacagttat atcaaaaata gtatcaagta gtctttctga agaagaagtt
     1201 gcttctggaa tgagtttgct aaatttcaca agttttttat cagagggaac aggtatagca
     1261 attgtaggag gtttattgtc actacaattg attaatcgta aactagttct ggaatttata
     1321 aattattctt ctggagtgta tagtaatatt cttgtagcca tggctatcct tattatttta
     1381 tgttgtcttt tgacgattat tgtatttaaa cgttctgaaa agcagtttga atagttatat
     1441 tatattttgg tttagaacta tgagtggcta gcattttgcc actcattttt tgcgttagca
     1501 aaaacaggtt taagcctcgc agagcacacg tattaacgac ttattaaaaa taagtctagt
     1561 gtgttagact taaactatta aatacacatg aaacctttgt gcttaggagt gatttttata
     1621 tgtcttattc cattgttaga gtttcaaaag ttaaatctgg aacaaataca acgggcatac
     1681 aaaaacatgt tcaaagagaa aataataatt atgaaaatga agatatagac catagtaaaa
     1741 cttacttaaa ttatgatttg gtaaatgcta ataaacagaa ttttaataac ttgattgatg
     1801 aaaaaatcga acagaattat acaggcaaaa gaaaaattag aacagacgcg attaaacaca
     1861 ttgatggttt aattacatca gacaatgatt tctttgataa tcaaacgcca gaagatacaa
     1921 agcagttttt tgaatatgct aaagagtttt tagaacaaga atacggtaaa gataatttat
     1981 tatatgcaac agttcacatg gacgaaaaaa caccacatat gcattatggc gttgttccaa
     2041 taactgatga tggtcgttta agtgctaaag aagttgtagg taataaaaaa gctttaacag
     2101 cgtttcaaga tagatttaat gagcatgtta aacaacgagg atatgattta gaacgtgggc
     2161 aatcaagaca agtaacaaat gctaaacatg agcaaataag tcagtataaa caaaaaacag
     2221 aatatcataa gcaagaatat gaacgtgaga gccaaaaaac agaccatata aagcaaaaga
     2281 acgataaatt aatgcaagag taccaaaaat cgttaaatac gcttaaaaag cctataaatg
     2341 ttccgtatga gcaagaaact gaaaaagtag gtggtttatt tagcaaagaa atacaagaaa
     2401 ctggaaatgt tgtaataagc caaaaagatt tcaatgaatt tcagaaacag ataaaagctg
     2461 ctcaagatat ttcggaagat tacgagtata taaagtctgg tagagcctta gatgataaag
     2521 ataaggaaat acgagagaaa gatgatttat taaataaagc agttgagcgt attgaaaacg
     2581 cagacgataa ttttaaccaa ctttacgaaa atgcaaagcc acttaaagag aatatagaaa
     2641 tagcgttaaa gcttttaaaa atcttactaa aagagttaga acgagtttta ggaagaaata
     2701 cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat ggtttagcag
     2761 gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag caacaagaac
     2821 aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat ttatgccgag
     2881 aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact
     2941 tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc ctgtaggttc
     3001 cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga aattcagaga
     3061 agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag ttttaactgt
     3121 ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga ccaagtcttt
     3181 tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga ccaaagtttg
     3241 tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg tatatttaga
     3301 tattaaacga tatttaaata tacatcaaga tatatatttg ggtgagcgat tccttaaacg
     3361 aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa tcattcaaat
     3421 catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa tagccggttg
     3481 gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat gacgatcgtt
     3541 ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt agagccccaa
     3601 ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca agaaaaagtt
     3661 tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat gcgtattgaa
     3721 tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa tataataagc
     3781 tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga agatgatttg
     3841 agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta tggtcgtaat
     3901 ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat tagaatttat
     3961 aataaaaaga aagaacgtaa agataatgca gatgctgaag ttatgtctga acatttatgg
     4021 cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg ctttagtgat
     4081 ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag agcaatagtt
     4141 tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc tagaacaaaa
     4201 tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat gaaatcgact
     4261 ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca tgaatttaaa
     4321 ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt aaactattct
     4381 aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt tatatgagg
//
