LOCUS       NZ_MF370188             6953 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Enterobacter cloacae strain Ecl-35771cz plasmid pEcl-35771cz,
            complete sequence.
ACCESSION   NZ_MF370188
VERSION     NZ_MF370188.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227661
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Chudejova,K., Papagiannitsis,C.C. and Hrabak,J.
  TITLE     Characterization of an Enterobacter cloacae isolate producing GES-5
            carbapenemase from a Czech hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Chudejova,K., Papagiannitsis,C.C. and Hrabak,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) Department of Microbiology, Faculty of
            Medicine and University Hospital in Plzen, Charles University, Alej
            Svobody 80, Pilsen 30460, Czech Republic
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/20/2023 02:30:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9
            CDSs (total)                      :: 9
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MF370188.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="Ecl-35771cz"
                     /db_xref="taxon:550"
                     /plasmid="pEcl-35771cz"
                     /note="GES-5-encoding"
     gene            join(6754..6953,1..19)
                     /locus_tag="HTO32_RS00005"
     CDS             join(6754..6953,1..19)
                     /locus_tag="HTO32_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_160387704.1"
                     /translation="MPTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEYCNFR
                     DEKALQTLLDSLYEDALAEATAWLASR"
                     /name="hypothetical protein"
     gene            1..339
                     /locus_tag="HTO32_RS00055"
     CDS             1..339
                     /locus_tag="HTO32_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_160387705.1"
                     /translation="MAGQPLKRIRSIKIRVSDAELERLREICPKAQLAEWMREQCLGVV
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            320..1774
                     /locus_tag="HTO32_RS00060"
     CDS             320..1774
                     /locus_tag="HTO32_RS00060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_233471716.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGSPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRANKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASERRVHEARESYLRGVEIKRGENERRY
                     CRAQPEYGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASQSPSLGGEGRQDKA
                     GPVREEVGAPLRADLRFQPGRLGEGYVLGGSEGVLNDGIGAGIVLRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQARGREQPGSGETLDVASGRLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKVLDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1957..2871
                     /locus_tag="HTO32_RS00025"
     CDS             1957..2871
                     /locus_tag="HTO32_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_160387708.1"
                     /translation="MTNRAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLNWLADYLDLTP
                     YNGRKQLPAYGLGRNCTLFEKTRQWAYKAIRQGWPGYREWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLTPAGFSAWQAEQGAKKGKALRDLMLPVVEELLNQGLAKA
                     DIARELGQSKATIGNWVKRLEIK"
                     /name="replication initiation protein"
     gene            2868..3062
                     /locus_tag="HTO32_RS00030"
     CDS             2868..3062
                     /locus_tag="HTO32_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172692818.1"
                     /translation="MTLHEIFVGEELEMEQFDLERIADLIYGWLEGEHIDPQMAHYAGM
                     LLLSLSQRALSFSEDDCTS"
                     /name="hypothetical protein"
     gene            <3032..3097
                     /locus_tag="HTO32_RS00035"
                     /pseudo=""
     CDS             <3032..3097
                     /locus_tag="HTO32_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /name="EAL domain-containing protein"
     gene            complement(3124..3915)
                     /locus_tag="HTO32_RS00040"
     CDS             complement(3124..3915)
                     /locus_tag="HTO32_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="WP_172692819.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
                     /name="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
     gene            complement(4038..4901)
                     /locus_tag="HTO32_RS00045"
     CDS             complement(4038..4901)
                     /locus_tag="HTO32_RS00045"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     GES-5"
                     /protein_id="WP_012658785.1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIVD
                     PQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVEWSPATE
                     RFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRKEPE
                     MSDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRAGFPK
                     DWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQVITQL
                     ILSTDK"
                     /name="carbapenem-hydrolyzing class A beta-lactamase GES-5"
     gene            5081..6094
                     /gene="intI1"
                     /locus_tag="HTO32_RS00050"
     CDS             5081..6094
                     /gene="intI1"
                     /locus_tag="HTO32_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_062939554.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLQTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     ARG             complement(4042..4901)
                     /name="blaGES-5"
                     /gene="blaGES-5"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     GES-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3128..3915)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA15"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.62"
                     /coverage="100.0"
     Typing          321..1771
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.562"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctggcc agccgttaaa gcgcatccgc tctatcaaga tccgggtttc agatgcggag
       61 cttgagcgcc tgcgggagat ctgccccaag gcccaactgg cggagtggat gcgggagcaa
      121 tgtctcggcg ttgtgcagcc ccagcgccga acgccggcgc ctaccgtaga tccggcgctc
      181 ttgcggcagt tggccggaat gggcaacaac ctgaaccaga tcgcccggcg cgtaaattcc
      241 ggggagtggg ggccgttgga tcggctgcgc atcatagcgg agctgtccgc tataggccgc
      301 gagctggagg aactgcacca tgatcatcaa attccataac cgtggggtag gccggggctc
      361 cgggcctgtt ggctacctgt tgggccgtga cggcaaccgg gaaggcgcaa cgctcgatcg
      421 gggcagccct gcggtgattg aagccctgat tgattccagt ccctacgcca aaaaatacac
      481 ctctggcgtc ctgtcgtttg ccgaggctga tttacccagg gcgaacaagg acaagctgat
      541 gagcgacttt gaaaaggcgc tcctgcccgg actggagggc aaccagtacg ctgttttgtg
      601 ggtagagcac cgcgacaagg ggcggctgga gctgaatttt gtagtcccga acatcgaatt
      661 gcagagcggg aagcggctgc aaccctactt ccacaaggcc gacaatccgc gcctcgatgc
      721 gtggcgaacc gtggtaaacg gggagctgaa attgcacgac ccggacgatc cgttaaaccg
      781 tcaggccgtt atcacgccaa aggatttgcc gagggggcac aaggaggccg ctcaggctat
      841 tacagacggg cttttgcgca tggctggggc aggggagatc cagagccgcg atgatgtgcg
      901 caaagccttg cagggtgcgg gtttcagcgt ggtacgcgaa accaaaaaca gcctgagcat
      961 tgccgacccg gacggggggc ggaacatccg actaaaaggg gtgctgtatg agcaagattt
     1021 tagacatggc gaaggacttc gagcagaact cgaagcagca ggccaacgct atcgagaagc
     1081 gtctgagcga agagttcacg aggcacgaga aagctatctc cggggcgttg aaatcaagcg
     1141 aggagagaat gagcgccgct attgccgagc acagccagaa tatgggctgg ctgttgctca
     1201 aaacgtggat ctggatgctg gtcagcatcg tcctgttctt gagcgcaacg agcgggatcc
     1261 tttggtatca gggggaattg atagcggagc ggcaagccaa tctccaagcc ttggcggaga
     1321 agggcggcaa gataaagctg gcccggtgcg ggaagaagtc ggagcacctt tgcgtgcaga
     1381 tctccgattc caacccggac gtttggggga aggatacgtt ttgggtggtt ccgaaggggt
     1441 actaaatgac gggattggag cgggaattgt tctgcgcgtt cgaggctttg cagagcgcat
     1501 acgagcagca gcacaaggca tggcaggaaa gctacgcgag cttggcgagc atgtacgaga
     1561 gcaccaagcg agaggccgag agcagccggg aagcggcgaa acgcttgacg tggcaagtgg
     1621 acggcttaac gcagcaagtg gacggcttga gcaagcagct ggagggcttg accgggctag
     1681 tcagcaagtt gcaaaagaaa gggaacacag gctcctgaag gtactggatc gaggctggga
     1741 gcgtggagac agatcgcgag gctttgagcg atagcgccca gtaggatcag gacgtttttt
     1801 ggtgtaacgt actgtgcaga aaagacaaac cccgaagctc tgctgatcaa cttggcggaa
     1861 gactcagagc attcggggcc gttcactaag aacaggaaaa gcataccaca tggatcagac
     1921 caaacaaccc cagaccgcca ataagcgcgg tggattttga ccaacagagc acagcttgag
     1981 ctgttccggg agcgcctgcc ccggaagcct tactattccg acgagcttac aacgggccta
     2041 cggattgcgg atgttgcccg cgcccttggt gcgcgttaca tccagccgaa cgggccaacg
     2101 caccgccact ggatcgtttt tgacgtagac catgccgccg ctacgttatc ttgggatgac
     2161 gtaggcgctc cagcccctaa cattaccgtc acgaacaagg ccaacggcca cgcgcatctg
     2221 atctacggac tggatacgcc gatccgcact gccccggatg gaaatgcagc ccctctgcgg
     2281 tatgccgccg ccatcgaggc cgcgctccgg gagaaattag gggcggatat gggctactcc
     2341 gggttgatct gcaagaaccc cctgcatgag cactggctgg tgcaggtatg ggagccccgg
     2401 ctctacgatc tgaactggct ggccgactac ctcgatttga cgccctacaa tggccgcaaa
     2461 cagcttcctg cctacggact ggggcgaaac tgcaccctat tcgagaaaac tcgccagtgg
     2521 gcttataaag cgatccggca gggctggccg ggttaccgcg aatggttggc ggcttgtgtt
     2581 gatcgggcat caggatataa cgcccagttc gagcagccgc tccccgcaaa cgaagtccgg
     2641 cacacggcca agtccattgc taaatggacg caccagcatt taacccctgc gggctttagc
     2701 gcatggcaag cagagcaggg agccaagaag ggcaaggcgc tccgggattt gatgttaccc
     2761 gtagtcgagg agctgctgaa ccaaggatta gccaaggctg atattgcacg agagctggga
     2821 cagtccaagg cgaccatagg taattgggta aaaagattgg agatcaaatg actctgcacg
     2881 aaatcttcgt tggtgaagaa ctagaaatgg aacagtttga tctagagcgt atagccgatc
     2941 tgatatacgg ctggctggag ggtgagcaca ttgaccccca aatggcgcac tatgctggaa
     3001 tgctcctttt gtctctgtca cagcgagcgc tgtcattttc agaagacgac tgcaccagtt
     3061 gattgggcgt aatggctgtt gtgcagccag ctcctgacag ttcaatatca gaagtgttag
     3121 acatcattta ccaactgact tgatgatctc gcctttcaca aagcgaataa attcttccaa
     3181 gtgatctgcg cgtgaggcca agtgatcttc tttttgtccc agataagctt gcttagcttc
     3241 aagtaagacg ggctgatact gggcaggtag gcgttttatt gcccagtcgg cagcgacatc
     3301 cttcggcgcg attttgccgg ttattgcgct gtaccaaatg cgggacaacg taagcactac
     3361 atttcgctca tcgccggccc agtcgggctg cgagttccat agcgttaagg tttcatttag
     3421 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac
     3481 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt
     3541 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc
     3601 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac
     3661 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa
     3721 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact
     3781 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg
     3841 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat
     3901 caccgcttcc ctcatgatgt ttaacgcctg agttaagccg cggtgcgcag tggcaagcgg
     3961 cgtgcgaagc ttagcgtagc acgcaagcgc cgccacgaag caacgtcggc ttgaacgaat
     4021 tgttagacgg gcgtcaacta tttgtccgtg ctcaggatga gttgtgtaat aacttgaccg
     4081 acagaggcaa ctaattcgtc acgttctacg gccgatagtt tcggggccgt tgtatacacc
     4141 gctacagcgt aatctctctc ctgggcttta aaaaaaccaa tgtcgttccg gcccccgttg
     4201 gcgcaggtac cagttttctc tccaacaacc caatctttag gaaaacccgc tcgtagtgtc
     4261 gcgtctcccg tttggtttcc gatcagccac ctctcaatgg tgtgggtcga ggtggacgtc
     4321 agtgcgccgc catagaggac tttagccaca gtacgtgcca tagcaatagg cgtagttgta
     4381 tctctgaggt cgccaggtgt gttgtcgctc atctccggct ctttccggtc tagccgactc
     4441 acagagtcgc caattttacg aaaatactgc gtcattgcag caggtccgcc aatttctctc
     4501 agtaagaggt tagtagcccc attgtcgcta agctgcaccg cagcttgcgc tgcctcgaga
     4561 accgtcatgt gtcccgatgc tagaaaccgc tccgtggcag gagaccattc gacgatcatg
     4621 tccggcccat atgaaagttt tcgatccccc cgctcggtgc ctgagtcaat tctttcaaag
     4681 accagcgcgg ctagcggaaa cttgaacgtt gagcacattg caaaacgctg cgccattcgg
     4741 tggcccgcga cgatctctcc ttggggatcg acgatcgcaa caccgatctg agctgctttt
     4801 tcgcgctcta gcttctcaag atcggtcttg aaggttaatt tttccgacgc atatgcagag
     4861 tgagcgatcc ctgccagtaa tagtgcgtga atgaagcgca tggtgatccc ttgagatgga
     4921 aattatcttt gtacgcccgt ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     4981 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     5041 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     5101 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     5161 gcattacagt ttacaaaccg aacaggctta tgtccactgg gttcgtgcct tcatccgttt
     5221 ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg
     5281 gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct
     5341 gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc
     5401 tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg
     5461 ttttctggaa ggcgagcatc gtttgttcgc ccagcttctg tatggaacgg gcatgcggat
     5521 cagtgagggt ttgcaactgc gggtcaagga tctggatttc gatcacggca cgatcatcgt
     5581 gcgggagggc aagggctcca aggatcgggc cttgatgtta cccgagagct tggcacccag
     5641 cctgcgcgag cagctgtcgc gtgcacgggc atggtggctg aaggaccagg ccgagggccg
     5701 cagcggcgtt gcgcttcccg acgcccttga gcggaagtat ccgcgcgccg ggcattcctg
     5761 gccgtggttc tgggtttttg cgcagcacac gcattcgacc gatccacgga gcggtgtcgt
     5821 gcgtcgccat cacatgtatg accagacctt tcagcgcgcc ttcaaacgtg ccgtagaaca
     5881 agcaggcatc acgaagcccg ccacaccgca caccctccgc cactcgttcg cgacggcctt
     5941 gctccgcagc ggttacgaca ttcgaaccgt gcaggatctg ctcggccatt ccgacgtctc
     6001 tacgacgatg atttacacgc atgtgctgaa agttggcggt gccggagtgc gctcaccgct
     6061 tgatgcgctg ccgcccctca ctagtgagag gtagggcagc gcaagtcaat cctggcggat
     6121 tcactacccc tgcgcgaagg ccatcggtgc cgcatcgaac ggccggttgc ggaaagtcct
     6181 ccctgcgtcc gctgatggcc ggcagcagcc cgtcgttgcc tgatggatcc aacccctccg
     6241 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacaagcg
     6301 ccagatctcc ctgatgcaca gtgaatccaa caaaaaactc gtcttttaaa aaagagtaaa
     6361 aaagccatat cagataatag ccccgttagg gggttcctgc gctcttgtct tttagccgta
     6421 aaaaatcctt ttagcgcgga tccttggcga ggtggacaac tgcgcctgtc cgtccatcgc
     6481 ccccctgttt tttcgggggg tgtgggcggc agcagttatc cacggagcag ctcttgccct
     6541 tgatctagac ctagggcttg cagtatcccg cccatagttg aataatgtac taagcaaggc
     6601 tacacagagc cccctctacc tgcttcgcgg tctcggggct ctgtggcctt gttcaggggt
     6661 gctacgcccc cccgaaaccc ccctgtggcc cccgctcgat ccgccccttc gggacggtct
     6721 gcgcggcggc ttcttggctc tggagaatgg acgatgccca cggaatacga ttacgaaatc
     6781 accccccgca ctctcgggct tggcgggggc tggaatctgc gtttgctgga gaacggcgaa
     6841 gaggtcgggg ggggtgtttt ccccttgccg gaatactgca attttcggga tgaaaaggcg
     6901 ctccagacgc tactcgatag cctctatgag gacgccttag cggaggccac cgc
//
