LOCUS       NZ_MF150119             9294 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain A64477 plasmid pKP64477e, complete
            sequence.
ACCESSION   NZ_MF150119
VERSION     NZ_MF150119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07137341
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Cassu-Corsi,D., Martins,W.M.B.S., Nicoletti,A.G., Almeida,L.G.P.,
            Vasconcelos,A.T.R. and Gales,A.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2017) Labinfo, LNCC - Laboratorio Nacional de
            Computacao Cientifica, Rua Getulio Vargas 333, Petropolis, RJ
            25651070, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Newbler v. 3.0
            Genome Coverage              :: 11200x
            Sequencing Technology        :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/09/2024 02:04:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MF150119.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="A64477"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP64477e"
                     /geo_loc_name="Brazil: City of Sao Paulo, State of Sao
                     Paulo"
                     /collection_date="2014-11-25"
     gene            271..2235
                     /locus_tag="KP64477e_RS00005"
                     /old_locus_tag="KP64477e_00001"
     CDS             271..2235
                     /locus_tag="KP64477e_RS00005"
                     /old_locus_tag="KP64477e_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            2235..2966
                     /locus_tag="KP64477e_RS00010"
                     /old_locus_tag="KP64477e_00002"
     CDS             2235..2966
                     /locus_tag="KP64477e_RS00010"
                     /old_locus_tag="KP64477e_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            2973..3503
                     /locus_tag="KP64477e_RS00015"
                     /old_locus_tag="KP64477e_00003"
     CDS             2973..3503
                     /locus_tag="KP64477e_RS00015"
                     /old_locus_tag="KP64477e_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3531..3710)
                     /locus_tag="KP64477e_RS00020"
                     /old_locus_tag="KP64477e_00004"
     CDS             complement(3531..3710)
                     /locus_tag="KP64477e_RS00020"
                     /old_locus_tag="KP64477e_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3779..4156
                     /locus_tag="KP64477e_RS00025"
                     /old_locus_tag="KP64477e_00005"
     CDS             3779..4156
                     /locus_tag="KP64477e_RS00025"
                     /old_locus_tag="KP64477e_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(4218..4517)
                     /locus_tag="KP64477e_RS00030"
                     /old_locus_tag="KP64477e_00006"
     CDS             complement(4218..4517)
                     /locus_tag="KP64477e_RS00030"
                     /old_locus_tag="KP64477e_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4507..4770)
                     /locus_tag="KP64477e_RS00035"
                     /old_locus_tag="KP64477e_00007"
     CDS             complement(4507..4770)
                     /locus_tag="KP64477e_RS00035"
                     /old_locus_tag="KP64477e_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(5076..5495)
                     /locus_tag="KP64477e_RS00040"
                     /old_locus_tag="KP64477e_00008"
     CDS             complement(5076..5495)
                     /locus_tag="KP64477e_RS00040"
                     /old_locus_tag="KP64477e_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5898..7583
                     /locus_tag="KP64477e_RS00045"
                     /old_locus_tag="KP64477e_00009"
     CDS             5898..7583
                     /locus_tag="KP64477e_RS00045"
                     /old_locus_tag="KP64477e_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7593..7850
                     /locus_tag="KP64477e_RS00050"
                     /old_locus_tag="KP64477e_00010"
     CDS             7593..7850
                     /locus_tag="KP64477e_RS00050"
                     /old_locus_tag="KP64477e_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            7935..8084
                     /locus_tag="KP64477e_RS00055"
     CDS             7935..8084
                     /locus_tag="KP64477e_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             898..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(8188..8766)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2236..2963
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact ggggtgccgc
       61 cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca
      121 tggggcggaa agaggctggc ctccgtggtg aaagctgaac gcaatcacca cggacgtgac
      181 cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg cacatcacgc
      241 cggcaaacca tcgtctgaag gagagagaac atgtttaata cggattcgct tgcctggaag
      301 tggcacattc cggagctggc ccggcagatt tatccgtggg tcagctggct gtaccaggag
      361 tattacggtc tggccggggc tttcctagtt atcggcctgt ttttcggtct ggctgaactt
      421 tgggccggac ggatgcgttt tgtggggctg agtcataagg agcgacggga gtatgccgat
      481 cgctataagc gcgataataa acggccagcg ccattttcgg tgctggcctc gctggattcg
      541 cccgggcgga tggtcagggc acgtcgacat gtctggaact acgcgtactg gctgaccggc
      601 gcggccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga gagctgggga
      661 gaaaccgtcc tgcgtccgga cctctacgca aacgcagatt attacctgct gcagccggct
      721 aacctgttcg ggattgcctg gtgcgcgggc gggctggctg tcgggattgt gttggcgcgg
      781 ctgctggcgt tcctgctgct tgacggtcat tttgcggctg ctgacgaggc ggtgaacgcg
      841 aaactgaatc aggagagccg gcgcagcagc cagcggacag gagagatgac cgacgtgcgc
      901 cacctgcatt tcggggagcc ggtaccggtt aatgcgctgg ccgatttttc ctcggagcag
      961 gcccggcagg agcaggcggt gtttctgggt aaggatgagc agggcaagcc ggtgctggtt
     1021 ccgcgtgata cctggcgcaa gaccaacatt cagatcctcg gcctgcccgg gagcggcaag
     1081 agcgtgatgg ccaccaacgc gctcattcgc tgcgtacgcg atttcgggga cgcggtggtg
     1141 tacttcgacc ccaaagggga cgcatgggcg ccgcacgtct tccgggcgca ctgtccggac
     1201 ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc
     1261 gaccagtacg cgctgaaaaa cctgctgatt gccgggctta acctgggcga gaccggcgac
     1321 gtggcggacc actaccgcat cagcgagcag aaagcggcga agctgattgc ggagcagttt
     1381 ccgcaggggg caaacattca gcaggtgctg gcggcggcgt acgcgctgcc ggaggccctg
     1441 aaaaaggacg tgaaggggtt gatcactagg ctggaaaacg tcgctgacct gagcgtcctg
     1501 cagacggaca gtggcatcga tgtggcgggg attatcaacg gcggcggctg cctgtacgtt
     1561 atcggctcga tggacgacga ggcggtgatc cgtgtgcaaa aaatgctgtt tgcccgctgc
     1621 gcgcaaatca tcattgcgcg ggacgagttc cgggagtggc cacacgtcag cgtgatgctc
     1681 gatgaaatta aatatctgct gtcgaagtat gtactgaacg cactggggac gctgcgcagc
     1741 cgggactgca atctgttgct cgcgcaccag tcgctgggcg atttcgggca gtgcgggcag
     1801 gatctgcccg ctgaatttgt gaaaaccacg gtactggata acacgccgat ccgctggttt
     1861 taccgggcgg ccagcctgga gtccgcgcag tgggcggcgg ggcagaccgg ggaaatcagg
     1921 gtggacgtgg agcgccgccg tgccagccgg gaggcgggga acgtggagca tatcagcggg
     1981 gacagcttca tccagaagga ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg
     2041 ccggacgggt tcgcggtcat gaccgggctg ggtgtggccc ggatggcgtt cagcagtccg
     2101 ctgcgggttg agcgccggga aatcccgttg cagcgtttcc cggtgctggc gaaaaccgat
     2161 cccctggcgg agttccggcc cccggccgac ggcggcgggc cgaaagcgga tgatgatttt
     2221 gcggggattt actgatggcg ctggagctgt acaacgtcag ccacgcgaaa cgacaggcgc
     2281 ggaacgcgga gaaaacccgg ctgaccctgc gctggttgcg ggaagagctg tgttccacgg
     2341 cggagctggt tgcgcggcgg ctggggattg cggcggtgca gccggtctac cgcttcctcg
     2401 acagcctggt ggcgaaaggg ctgctggtcc ggaccaaata tccggtggac ggccgtcagg
     2461 tcagcgtgtg ggggctgacg ccgcatggcg tggcgttctc gttcgacgag gacgaaccgc
     2521 tggcggatgt tattccgttt cagccgtcaa gggtgtcggc agcacatctg ccgcaccggc
     2581 tggccgtgca atccctgcgc cttgatatgg aagccagagg ggccaccggc tggcgcattc
     2641 tgcaccgtct gagtcttaaa ggcatgaaag tgccggacgc cctggcggag ctggacggta
     2701 aaacggtggc gtttgaggtg gagcgcaccg tcaagagccg tcgccggtat caggaagtgg
     2761 tgtccgggta tctgttcaac cgcaaagcta acggcatcga tgaaatctgg tacatctgcc
     2821 cggaccgggc gacgcaggtc agggtgcagc gggcaatcct gtcggtggat gaaatcgtga
     2881 acccacagac cggcgaggcc cgcaaaacgg cagagctgga tcgtgaacgg ttgtttgcgt
     2941 gctttaaatt tatgacaacg gagtgaggga ttatggcgct gtacatggta tttattgaag
     3001 acgcggagga ccggaacggc gagcgctgca tcatgccgac cagcgagcat ctggaagata
     3061 tggtggtgct cgcctggcag accggggcgt atgttttctg ttttgtcgac cgggcccagc
     3121 atctggcgat gatgaaatcc atcgggatgt atgacgagcg gctcaacagc gacgaaaaag
     3181 ccacgtactt tttcactggt aaaaatccgc aatgggaacg gaacaaaccc acaggaatga
     3241 agctgtgtga ttacctctat cgcaaggtgg agcggttcgc catcgagaac ggtgtgcagg
     3301 tggttgatgc cgggaagtgc tcaccggagc agattaacgc gatgattgac gtggtgagtg
     3361 agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc ggagctgatg cgggaatata
     3421 acagccgcta ttttaacgtg ccggtacctg aactgccgga ggacaaacct gcccgccggt
     3481 ggtggccgtt cggcaaaaaa taaatgccag caccggccat gccggtgctg ttattcctcc
     3541 ccgagcttat cccgcatcac ctgccagagt gtttccgcat cctcgtgtag ccgctcgcac
     3601 atgtcggccg gggtatccat atccagctca ttcagtttat cgagcaatag cagcgactgc
     3661 tccttaataa atttagccat attgagggct gttttttcct gttttgtcat gattctttcc
     3721 ttccgggcat ggtgtgcgcc tgtcaggtat actgtctcaa aaatagcggg gtgagacaat
     3781 ggcgtgggtg acggtgagac aggcgacgga actgacgggt aaagcccgaa gttccctgta
     3841 tcgggatatg gcgaaaggcc gcgtatcata ccgtacggaa gctgatggcg gccgggtggt
     3901 ggataccagc gagcttatcc gggtgtacgg tgagttaaga caggatgaga ctcacgagcg
     3961 ggacgagttg agactgtcgg gtgagactga aaaaatggcc tccgacgcgt tgatcgcgga
     4021 gataaaagca ctcagggagg aggtggccgg gctacggcag gaaatgcagt ccatgagact
     4081 gctggaacac aaaccggaga cagcagagac cccggccgga acaccgcgcc ggtggtggcc
     4141 gtggggaaaa cgctgacgcg ctgtgcttgt ccaaccggcg acggccacgc aatgaattgc
     4201 gtccccgtca ccggttatta gagaacaata tggggattct ggcgctggcc gaccacggcc
     4261 agaacccgga cttttctggt ctggtcatca acatcataga ggacatgctg tcccatcaga
     4321 ctgatccggc gaacgtcctc gccatagcgt gagtccactt catagagtcg cgggaagtcg
     4381 gccagtttgt ccgccgcagc gagaacccgg tcggtaacgt tctgcgcatc ccacagggat
     4441 gaattttccg cgatatagtc aaacagcgtc gttacatcct cgatggctcc ctgcttccat
     4501 tccacgtcat acggcatcgc gcagttcctg ataacggcca tgcaggcgct ggcggagttg
     4561 ttccgtggtc atacccggac gcgtgtcagc acgggcggcg gtcactttgg cgcgaacctg
     4621 atcttcatac tctgcctggg ccagttcttc gttagccagt cgctgcaaca gcgcagcttt
     4681 gtccgggcca ccttccggca gtatgcgggg caggctttca gccagttccc gaagaaagtt
     4741 ggcgttttcg tagtgtgtag tgttgctcat tgcggagcct cccgggttaa tgcggcaata
     4801 tgaaggaagt ttagcagggg gcggtctgaa agaatatggt cgtgtcagtc aggctgatga
     4861 agcagaaagt cggtaaaaaa gggcttacgg catccatttt acgtcaaaac atatgccatg
     4921 cagttgcgtg cataggcatg cattaggata aaatttatcg ggcgcgtttc cggcagtttt
     4981 ccgggggggt tgttgcctgt tttatcccgt agccgccgga aacgccctga gcccgtctga
     5041 gcggtgcgcg taatgacgcg ttatggtaaa tccacttact gtctgccttc atagccatcg
     5101 agataaacag ctgaaatggt gtcagccagt agccttgatt ttttcggtgt gaggtctttg
     5161 ctgcggtaat cggtacccgc aaattgtgtt gtgccttgga gtgagtttaa aaattcctga
     5221 ctgtgaaact gctgaatgcg ttgctgcaca tcactccgtg attttccggt cgctttatcc
     5281 tttgcgccca tatcccagaa ctgccggaaa aaaggcacca tcattcgtgt tgtgtcagct
     5341 ttctgggtgg aaaaattggg atcaaaaccg tcatctgaag catatacgta atgccgggta
     5401 tgctgttcca cgctgccctt tggagcacag gcagtgagag ttgctgtcgc tgttatcatg
     5461 gcgataagac acagtttgct gccggaacgt ctcatgagcg tctccttacc tttaattctt
     5521 ttttctcatt gagttatata tccgcaggct atcttatcat cagtttctga ccgtgttcag
     5581 tgaccgaaca acccgaacca gaccccccgt ttcttttcct gtcccggagg gggctttctg
     5641 gtatctcata taatgatttc agtaaaacat actgaggccg gagaacggcg ccgtttgtgc
     5701 tggtttttgt tgcatctgga ggggattgcg catggtgtga gtggctgttt ttttgatcat
     5761 tttcacaaaa acgcaaattt acagacttga catgaaaaac aaggcagcgt aatttttata
     5821 ctgtatataa aaccagtggt tatatgtaca gtattttatt ttagtttatt gttttaaaag
     5881 tcaaagagga aaagtatatg agtggcggag acggacgagg tccgggtaat tcaggtctgg
     5941 gacacaatgg tggtcaggcc agtgggaatg tgaacggtac atcaggtaaa ggtggccctt
     6001 catcaggtgg tggtacggat ccaaacagcg ggccgggctg gggtacgacg catacaccta
     6061 acggggatat tcataactat aacccggggg agtttggtaa cggcgggagt aaacccggtg
     6121 gtaatggcgg taacagcggc aatcatagcg ggagctctgg tggcggacag tcttcggcca
     6181 ccgcgatggc cttcggtctg cctgccttgg ccactccggg ggctgaagga ctggctttat
     6241 ccgtttccgg cgatgcgttg tcggccgctg ttgctgatgt gctggctgcc ctgaaaggtc
     6301 cgtttaagtt tggtctgtgg gggattgcga tttacggagt gctgccttct gagatagcaa
     6361 aagatgatcc gaaaatgatg tcaaaaatcg tgacgtcatt gccggcagac acggtaacgg
     6421 agacgccggt cagctccctg ccgctggacc aggcgacggt cagcgtcact aaacgtgtgg
     6481 cggatattgt gaaggacgag cggcagcata ttgcggttgt caccggccgg ccaatgagtg
     6541 ttcctgtggt ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg tcgattccgg
     6601 gtctcccgtc tctgcaggtg agcgtaccta aaggtgttcc ggccgcgaaa gccccgccaa
     6661 aaggcattat tgctgaaaaa ggtgattcac gtccggctgg ttttacggcc ggtggtaact
     6721 cccgtgaagc cgttattcgt ttcccgaaag agagcggaca gaaaccggtc tatgtgtcgg
     6781 tgacggatgt tcttactccg gcacaggtaa aacagcgtct ggaggaagaa aagcgtcgcc
     6841 agcaggcatg ggacgctgct cacccggaag aggggctgaa aagagagtat gataaagcga
     6901 aagctgagct ggatgctgaa gataaaaata ttgcgacctt aaacagtcgc attgcatcga
     6961 cagagaaggc gatccccggt gcaagggctg ctgttcagga agccgataaa aaggtaaaag
     7021 aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa tatggctccg
     7081 ggtggcagga tcaggttcgc tatcttgata aggatattca gaatcagaat gagaaattaa
     7141 aagctgccca gacatcttta aacgaaatga atgaatcctt atccagggat aaggctgcgc
     7201 tttccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata
     7261 aattaaatga ggaaaagaaa aaacctcgta agggaactaa agattacggc catgattatt
     7321 ttcctgatcc caagactgaa gatattaagg ggttgggaga gttgaaagag ggtaaaccta
     7381 aaacccctaa acaaggtggt ggtggtaagc gggcccgatg gtatggtgat aaaaagcgta
     7441 aaatttatga atgggattcc cagcacggtg agcttgaagg gtaccgcgcc agtgatggcg
     7501 aacacctcgg ggcattcgat ccaaaaacgg gtaagcaggt taaagggccg gatccaaaac
     7561 gaaacattaa aaaatatctt taagaggtaa atatggggct taaattacat attcattggt
     7621 ttgataagaa aaccgaagag tttaaaggtg gtgaatactc aaaagacttc ggtgatgatg
     7681 gttctgtcat tgaaagtctg gggatgcctt taaaggataa tattaataat ggttggtttg
     7741 atgttgaaaa atcatgggtt tcgatattac agccacactt taaaaatgta atcgatatta
     7801 gtaaatttga ttactttgta tcctttgttt accgggatgg taactggtaa gcaaaagtta
     7861 tcaaggatga tttgaaatac agacttccgg tgttcaggga tgaataccgg ggtttttaaa
     7921 tacagggaag gtatatgaaa aaggtaaagg ttgtttttct ttttatattg attttttcag
     7981 gctttcttct ggtcgcatgt caggcaaact atatccggga tgttcagggt ggaacggtgg
     8041 caccatcgtc ctcctctgaa ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc
     8101 ttcttgagat cctttttttc tgcgcgtaat cttttgccct gtaaacgaaa aaaccacctg
     8161 gggaggtggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgt ggtaactggc
     8221 tttcgcagag cacagcaacc aaatctgtcc ttccagtgta gccggacttt ggcgcacact
     8281 tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg aactcccagt taccaatggc
     8341 tgctgccagt ggcgttttgc gtgcttttcc gggttggact caagtgaaca gttaccggat
     8401 aaggcgcagc agtcgggctg aacggggggt tcttgcttac agcccagctt ggagcgaacg
     8461 acctacaccg agccgagata ccagagtgtg agctatgaga aagcgccaca cttcccgcaa
     8521 gggagaaagg cggaacaggt atccgggaaa cggcagggtt ggaacaggag agcgcaagag
     8581 ggagcgattc atcggaaacg gtgatgatct ttaagtcctg tcgggtttcg ccactcctga
     8641 ctgattcatg gttgagccac cggctcccac agatgcaccg aaaaagcgtc tgtttatgtg
     8701 aactcagtca ggagggcgga gcctatggaa aaacgccacc tgcgtggcct tgccgttttg
     8761 cctcacatgt tagtcccctg cttattcacg gaatctgtgg gtaactttgc atgtgtccgc
     8821 agcgcccgcc gcagtctcac ggccggagcg tagcgaccga gtgagcgagg aggcgcaatt
     8881 tccccggcac ctacaagacg gacaccggac gcgctgatgt ggcatattgt cccctgatta
     8941 agtctttata ttccctgata ataaattacc aggataggtg aatcatatcg gtttttcgct
     9001 gaacatttta cgttaaccta tctctgttgc ttttttctcc ttacggagaa atacaggtca
     9061 tccccgcgag ggcgtggctg acctcagggg gatatcccct gcttacgctg gctggacgcc
     9121 accgacgcac ggtctctccc cctctttctg gctccgtccg gctggacgcc ggccacgcca
     9181 gaaattcacc cggcccccgt caggctcacg cctgaccacc cacaaaaatc cacggtgctg
     9241 gacgcacctg gcgaagcagc aaagctgctc ctgattttgc gggtggtcag ccgc
//
