LOCUS       NZ_LT985386             6859 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain 694 plasmid RCS55_pII, complete sequence.
ACCESSION   NZ_LT985386
VERSION     NZ_LT985386.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588839
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900290955.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:22:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 126
            CDSs (total)                      :: 126
            Genes (coding)                    :: 117
            CDSs (with protein)               :: 117
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985386.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="694"
                     /db_xref="taxon:562"
                     /plasmid="RCS55_pII"
     gene            <3..74
                     /locus_tag="GPJ62_RS00660"
                     /pseudo=""
     CDS             <3..74
                     /locus_tag="GPJ62_RS00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(290..445)
                     /gene="csl"
                     /locus_tag="GPJ62_RS00005"
     CDS             complement(290..445)
                     /gene="csl"
                     /locus_tag="GPJ62_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="WP_071703583.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            475..1013
                     /gene="csi"
                     /locus_tag="GPJ62_RS00010"
                     /pseudo=""
     CDS             475..1013
                     /gene="csi"
                     /locus_tag="GPJ62_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /name="csi"
     gene            complement(1041..2540)
                     /gene="csa"
                     /locus_tag="GPJ62_RS00015"
                     /old_locus_tag="RCS55_PII0001"
     CDS             complement(1041..2540)
                     /gene="csa"
                     /locus_tag="GPJ62_RS00015"
                     /old_locus_tag="RCS55_PII0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="WP_104807859.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            2779..3069
                     /locus_tag="GPJ62_RS00020"
                     /old_locus_tag="RCS55_PII0002"
     CDS             2779..3069
                     /locus_tag="GPJ62_RS00020"
                     /old_locus_tag="RCS55_PII0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024216191.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            complement(3061..3339)
                     /locus_tag="GPJ62_RS00025"
                     /old_locus_tag="RCS55_PIICDS3070R"
     CDS             complement(3061..3339)
                     /locus_tag="GPJ62_RS00025"
                     /old_locus_tag="RCS55_PIICDS3070R"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            3461..3883
                     /locus_tag="GPJ62_RS00030"
                     /old_locus_tag="RCS55_PII0003"
     CDS             3461..3883
                     /locus_tag="GPJ62_RS00030"
                     /old_locus_tag="RCS55_PII0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024216190.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4366..4599)
                     /locus_tag="GPJ62_RS00035"
     CDS             complement(4366..4599)
                     /locus_tag="GPJ62_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4606..5148)
                     /locus_tag="GPJ62_RS00040"
                     /old_locus_tag="RCS55_PIICDS4609R"
     CDS             complement(4606..5148)
                     /locus_tag="GPJ62_RS00040"
                     /old_locus_tag="RCS55_PIICDS4609R"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_231201172.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVTGG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5201..5881)
                     /locus_tag="GPJ62_RS00665"
                     /pseudo=""
     CDS             complement(<5201..5881)
                     /locus_tag="GPJ62_RS00665"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5844..6167)
                     /locus_tag="GPJ62_RS00050"
                     /old_locus_tag="RCS55_PII0005"
     CDS             complement(5844..6167)
                     /locus_tag="GPJ62_RS00050"
                     /old_locus_tag="RCS55_PII0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6235..6426
                     /locus_tag="GPJ62_RS00055"
                     /old_locus_tag="RCS55_PIICDS6202D"
     CDS             6235..6426
                     /locus_tag="GPJ62_RS00055"
                     /old_locus_tag="RCS55_PIICDS6202D"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024192883.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(<6581..6796)
                     /locus_tag="GPJ62_RS00610"
                     /pseudo=""
     CDS             complement(<6581..6796)
                     /locus_tag="GPJ62_RS00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          complement(6512..6599)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4260..5854)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6227..6429)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.765"
                     /coverage="100.0"
                     /evalue="1.38e-10"
ORIGIN
        1 ccggtaacta tcgtctcgag tccaccccgg taagacacga caaagcgcca ctggcagcag
       61 ccattggtaa ctgaaaagtg gatttagata cgcagaactc ttgaagttga agccttatag
      121 cggctacact gaaaggacag catttggtat ctgtgctcca cttaagccag ctacccggtt
      181 aagcagtccc caactgactt aaccttcgac taaaccgcct ccccaggcgg ttttttcgtt
      241 tacagggcaa gagactacga cgatctgtaa aggatctcaa gaagatcctt tacaggctac
      301 cactaccacc acttcccata ctcactccgg taacagtgga agatggtgaa acagccccac
      361 ctccagtatc tctgacattg ttgacttggc atgctgcaag cagcattatt cctagtgaaa
      421 tggcacaaat aataattttt ttcatatata cctttattat aatcctatag gtcaatgatg
      481 attgatgaac attcaataga tatagacaat agaaaagcca ataacttatt gtatttattt
      541 atggcaatcg gagttatacc tttactctgt atactagccg tttactatac aaatccagac
      601 aatctgttct tgcacacaat agctaccagt actgaaaaca tcccatcaat aacctccgcc
      661 tacaacccat taatgacaaa ggttatggat atttattgta aaacagcgcc ttcccttgct
      721 ttgatattat ttatcctgac atttaaaacg agaaagctaa taaaaaaaca aaccgaaatg
      781 ctgtacttag aagttgctta ttaagtccat ttgtctgtgc tgtttttttg tatttactct
      841 gtttccgaaa ccttgagcta acgacagcag gaaggccagc caggctaatg accaacaatg
      901 atgcaacact attgctattt tatattggcc tgtacttaat catttttttc ataagctatt
      961 tcacgctatt cacaccagtt accacgttta aattattaaa agaaaggcag taaccctgcc
     1021 ttccttttac ccggtattta ttaatgagcg ggtttaatga tttctttatt tagtgcatct
     1081 gcaaacttgt catcaattaa tgcacctaca actgctgcga gtagaatacc tacgataccg
     1141 acagcaatag caggagcccc aagagataag gcatatgccc ctaatgtggc tgaaaaaaca
     1201 cccagggcca cagcagaagc gatgccactg agcacccagg actccacctc cagcatcaga
     1261 ggtccccagt tcccggtttc atagccctcg atgcttttct cccggacctt ctcaaccttc
     1321 atcaccacat cggcaaattt gaaagccttg ctgatattac ccagtttatt tgccatatcc
     1381 tgagcatcaa tacttcgcca gacgttcgcc agtgcatcac tgtcggccct gttaaattta
     1441 aaccccggat tagacaaaac tttatttaat gacgccattg catcatcgta gctacggata
     1501 gttttcccct ggaaattttt gacatcatca gcaatttctc ttgctaacga cttataagca
     1561 tctccaagat aatcaccgac cttattacct atatcagaga taacttcagc ggctttatta
     1621 agtacatcac tctgaatccg gtcacggttc atactgaaag aataaaacac atcataagca
     1681 gcttttttag cttgatcctg tcgttgaggc tcttcattat taaatcctaa tgcctcatat
     1741 atctcttccg gggtcttatg gtcgacttta tccataatac cagccttagc tttttcactc
     1801 cctacataag gacgaacagc tgttctagtt acagataaaa cattaccata atcatcaagc
     1861 accactaaat agtggaactg ttcaacatca acatcaaatt ttctcacatt aggaggcaaa
     1921 cgatcccacg catcttcagc ctgttgtttt gctttttcct gccgctgaga ttcatcttta
     1981 ttaaaaccta acgcctcata tatctcctca ggagttttat gattgatttt ttccataata
     2041 cccgccttag ctttttcact ccctacgtaa ggacggacac ctgttcgaag aataccgata
     2101 gctctaccat tatcattaag cgttacacga tatttaaaac catcgatact cacatcaatc
     2161 tctcgcggta agttgttttt atcccactta cgagcaactt catcattagg attttcaggg
     2221 atatagcccc cctgaacatc actatttccg ccagccccag gaactccata tacaggcgtt
     2281 tttaccagac catttgtgat ggtaatgcgc acctgcccat tatcatcaac ataaggcggc
     2341 acatccggat tattggtttt actccatccc caccccgtct gacttccatg ttcacgacct
     2401 ccattaccag agcctgaacc accaccccag tgaaccccac tattactatt attatttcca
     2461 ccttgctgat tcagattcgc ccctgttccc cccatagact caccagcagt tgggccatat
     2521 ccacttagtt ctttagccat aaattcctct ttgataatta aaacaataaa ttaaaaacag
     2581 aacactgtac atataaccac tggttttatg tacagcaaaa cctactactc actattgtcc
     2641 atgtcaagag catggatttt catttttgct ataaggatca cgctatgggg aggcaggcat
     2701 tgagaacgtc gaaacagaac accggagcaa atcaggatga gatataaaac tgttggatca
     2761 tgaaaaacgg agaacgatgt gagcaaatca ccccgccata aactgaacaa aacagacaaa
     2821 cgacttctcg acacccttgt tgctgccgga tatgagcatg acaaagcccg tgacctcatc
     2881 cagaaacagg tttacacgct gacactggct gatcagcgtc atgtggtcag tgaaatcagt
     2941 aatggtgtga atcccaccca ggcttactcg gcggtatacc aggcaagacg cattcgcctc
     3001 gcccgtaaat atctgaacgg taaaaaggtt atggaagaaa ccggggaaaa tacgccccca
     3061 tcaacgtaag gatttctttt gccgctccag agactccagt tttttacgca aatcctctct
     3121 tttttgggca tctctggtgc cagccagctc tgctctcaac tcatcgatct gaagttgtat
     3181 cttcagtctg ttactgaaca ttttctgtct ggcattaaca tccgcaacaa tgccgttttt
     3241 tgtcttctcg gccttttgtt gaaaaacatt gctgtccgca tgactggcaa ccgaagcaga
     3301 aagaacacta aaaagcagga ctggcacaca ttttttcacg ggattattcc tgactcattg
     3361 accatcaaat cacattggga gtaaaccgac gtatgataag agatactctt cggagatata
     3421 actccctgag tatcaagatt aaaaacgcaa ggagatgttt atgagatctg ccgctaccag
     3481 gctgcttctg atacctctga taacagcaac aatagctctt acaggatgca ccccaaagac
     3541 cagcctggaa cgacataccc gacattatgt ttatgcttca gatgatggat ttgatcctaa
     3601 cttctatacc cagaaagcag acaccatacg tatgatgctc ccgttctttc agcagttccg
     3661 ggatatgggg atgaaagaca aagcagccgg agtatcagca gaaacggcac agcaacgtgc
     3721 aaaagaattc cactcagaaa aattttttca ctcactccag agcacaacaa cctttgctgg
     3781 cagaaaatac acaaacagcg atatgccttc gctgaaaaaa atgaaactaa tggcagacac
     3841 catttctgcg gtttatctcg atggatacga gggcagacag taagggattt accataatcc
     3901 cttaattgta cgcaccgctg aaatgcgttc agcgcgatca cggctgctga caggtaaaaa
     3961 tggcaacaaa ccacccgaaa agctgccgcg atcgcacctg ataaatttta accgtatgca
     4021 tagctattca gccatgtgaa taacgctggt tttgcctgcg taaacctcat gacactgttt
     4081 ttttccatct tttcagttga tgacatacgc agacatcgcg ggatgaggct gaggaatgag
     4141 cgcgatctgg caaagaggca aaacacagca acaaaaacga cacgccagaa tcgcgcccgg
     4201 atgcgttttt aacgcgttcc ggtaccatct ggcaacctcc cggaacaact caccgtcaca
     4261 tccccattga cgggccacgc catacctgtg cttcccgttc ctgctcttca tgccaggacc
     4321 gcgcacgctc ccgttccagg cgtgcctgcc tttcctgttc atcccttatc tgctgttcgt
     4381 gataaataac cgactcaagc ggtccacctg cccggctaat ctctgcacct gctgactcaa
     4441 gtcgtcgcac tgttccctca gttgcccgtt ctcctgtcgt gtcagctcga acatatgctg
     4501 caaatccgtg aaggcgctct cccagtcttt cagccgctgc gtatagtcct gctgcaattg
     4561 ctctaaggcg ttcagtaagt gcatttccag ctctgtcata ctcacttact ccctgaccag
     4621 tcttactgcg ttcttcttct ccaccgtcca gttgttttcc cccttcaccc cggacggcag
     4681 caccagaaat ttcccgttcc tgccctcgtg atacgtcaca ccccatgttt tttcccggag
     4741 tttcgccagc gtctcttcct ggtccctgat agccaggatg ttcgccgcaa tccggctttc
     4801 ctgccactga atcagccccc atgacgccag aaaaatcccg cccgtgacgc aaagcgccgt
     4861 cagcgacggg tacagtatcc gccctttgat cagcttccag agcagctctt cctgccgccg
     4921 ggccagttcg ttctctgtgg cgctgaactg cgcgttcacg gcactgttca gcgtctccag
     4981 ttgttctttc accgctgctg tgtgtgcgct gatagcgtct ctgattttct gcccgtttaa
     5041 gttcagttcc ctgtctacag acgcttcgag cttcctgaac tcgctgttca gcatgttctc
     5101 tgtagagacg gcacgctctt tcagtttctt ctcgaagtct gtccccattt gtaaaagatt
     5161 gctcatacag cgcccctttc agcctgagat tacgcccgcc ctccgggtcg gcgatactga
     5221 tactgctcct ggttgtcctc acaacctcaa aacctgccgc tgtaagcgcc tcagtgacat
     5281 cctgacgcgt ttttagcgct ccggcatggt aaagagcctc cagtcccctc gtaatcgctt
     5341 ctgcggcctc ctgtttcgct ttcggcagat tattcggggt gacaagtgtc cgcctgttct
     5401 ccggtgcgtt cgggtcgtgc agcccgtaat ggtgattcac cagtgtctgc caggcattga
     5461 ttcgcggacg gtccgcccgg tcgtaatagg gctggagccg ttttccgctc gccagctcca
     5521 tattcgggat gacaaaattc agctcaagac gccccttgtc ctggtgctcc acccacagga
     5581 tgctgtactg atttttttca agaccgggca tcagtacccg ctcaaagctc tccatcaccc
     5641 tttcacgctc tcccggtggc agggtctgct ctgcaaaaga cagaaccccc gaggtgtatt
     5701 ttttcgcaaa cggcgtggca tcgatgagtt cccgtacctc ttcgggagca ccccgcagaa
     5761 ctctcgcccc ttcccggtta cgctcccggc ccagcaggta atcaaccgga ccactgccac
     5821 cgccttttcc cctggcatga aacttaacta tcatcccgtt ctccctgttt acggacctca
     5881 tccctaagct cactcagttc acgtccgatg gccatcagtg cagccaccac atgaacccgg
     5941 tcatgccccg accactgtcc gctgtttatc ttcctggcta tctgattcag gttattgccg
     6001 accgaagcga actggcgcaa cagcggcggt gccagtgtcg gaagacctga cgttttcgat
     6061 ggcggtgccc ccaggcagac cttacgcatc catgacgcaa gttgttttcc ctcacaacgt
     6121 gccagcagcc gcgcatgttc ctcatccgtg acccgtatcg tgagcatcct ttcgcgtttc
     6181 accggtatca ttaaaaacct ccgacagact ccccacacat ggagaaacag aactgtgact
     6241 aaacaggaaa aaaccgccct taacatggcc cgcttcatca gaagccagac gctgaccctg
     6301 ctggaaaaac tgaatgaact ggacgtcgac gaccaggctg acatctgcga agcgcttcac
     6361 gaccacgctg acgagcttta ccgcagctgc ctcgcacact tcggggatga cggtgaaaac
     6421 ctctgacaca tgcagctccc ggagacggtc acagctggcc tgtgagcgga tgccgggagc
     6481 agacaagccc gtcagggcgc gtcagcgggt tttagcgggt gtcggggcgc agccatgacc
     6541 cagtcacgta gcgatagcgg agtgtatact ggcttaatcg tgtagcatcg gtgaggattg
     6601 tatgaaaagt gcaccatgcc gggtgtgaaa tgccgcacag atgcgtaagg agaaaatgct
     6661 cgtccaggcg cttttccgct tcctcgctca ctgactcgct gcgctcggtc gttcgactgc
     6721 ggcgagcgga gtctgctggc tcaaaaacgg cgatacggtt atccacagaa tcaggggata
     6781 aggcagaaaa gaacatgtga gcaaaaaggc aaagggcaaa aaaagccgcg tagctggcgt
     6841 ttttccatag gctccgccc
//
