LOCUS       NZ_LT985314             6222 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain ECOR 25 plasmid RCS92_pI, complete sequence.
ACCESSION   NZ_LT985314
VERSION     NZ_LT985314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588853
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900290045.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:49:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 15
            CDSs (total)                      :: 15
            Genes (coding)                    :: 14
            CDSs (with protein)               :: 14
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985314.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECOR 25"
                     /db_xref="taxon:562"
                     /plasmid="RCS92_pI"
     gene            500..658
                     /locus_tag="GWA84_RS00100"
     CDS             500..658
                     /locus_tag="GWA84_RS00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1118..1933
                     /gene="sul2"
                     /locus_tag="GWA84_RS00055"
                     /old_locus_tag="RCS92_PI0001"
     ARG             1119..1930
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1994..2797
                     /gene="aph(3'')-Ib"
                     /locus_tag="GWA84_RS00060"
                     /old_locus_tag="RCS92_PI0002"
     ARG             1995..2794
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2797..3633
                     /locus_tag="GWA84_RS00065"
                     /old_locus_tag="RCS92_PI0003"
     CDS             2797..3633
                     /locus_tag="GWA84_RS00065"
                     /old_locus_tag="RCS92_PI0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<3740..4144)
                     /locus_tag="GWA84_RS00070"
                     /old_locus_tag="RCS92_PICDS3608R"
                     /pseudo=""
     CDS             complement(<3740..4144)
                     /locus_tag="GWA84_RS00070"
                     /old_locus_tag="RCS92_PICDS3608R"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4188..4610)
                     /locus_tag="GWA84_RS00075"
                     /old_locus_tag="RCS92_PI0004"
     CDS             complement(4188..4610)
                     /locus_tag="GWA84_RS00075"
                     /old_locus_tag="RCS92_PI0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4795..5235
                     /locus_tag="GWA84_RS00080"
                     /old_locus_tag="RCS92_PI0005"
     CDS             4795..5235
                     /locus_tag="GWA84_RS00080"
                     /old_locus_tag="RCS92_PI0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(6002..6151)
                     /locus_tag="GWA84_RS00085"
                     /old_locus_tag="RCS92_PI0006"
     CDS             complement(6002..6151)
                     /locus_tag="GWA84_RS00085"
                     /old_locus_tag="RCS92_PI0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             2798..3630
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2..138)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.275"
                     /coverage="100.0"
                     /evalue="4.41e-64"
     Typing          62..667
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tagcggagtg tatactggct tactatgttg ccacgattgc gaaaatcagt gaagtgcttc
       61 atgtggcagg agaaaaaagg cagcaccggc gcgtcagcag aatatgcgat acaggagata
      121 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc gagcggtacc
      181 ggcttacgga cggggacgga aatttcctgg aagataccag gaagacactt agcaaagaag
      241 agataaggcc acggcgaagc cgtttttcca taggctccgc ccccctgaca agcatcgcaa
      301 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat cccaggcgtt
      361 tccccctggt agctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
      421 tccgctgtta tggccgcggt ttctcattcc acgcctgaca ctcagttccg tgaaggcagt
      481 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttgtc
      541 cggtaactat caacttgagt ccaacccgga aagacacgac aaaacgccac tggcagcagc
      601 cattagtaac aggagtgcac gagaagatag attaagaggt cttgaagtag aggcctgact
      661 gcggctacac tgaaagaaca gttttggtaa tggcggtctc gtgaaccctg ttaccgggtt
      721 aagcagttcc ccaactgact taaccttcga acaaaccgcc tccccaggcg gttttttcgt
      781 ttacagagca agagattacg attcattgaa aaggatctca agaagatcct ttgtagatta
      841 ctatcaatta atcttccgaa catgacacca gtcactgttg attacggtta aaccgctccg
      901 taacaggttt ccctcgtatt ggtgacttgc agcaccaaag aagcaaaaaa gataaatata
      961 tctacttatt gagagatgat atatttattt ccaaaatgct acagatacat catcatgaaa
     1021 cggatcaccg caaacaggtt actcgcctgg ggattccctt tcgacccgag catccgtatg
     1081 agactcatgc tcgattatta ttattataga agcccccatg aataaatcgc tcatcatttt
     1141 cggcatcgtc aacataacct cggacagttt ctccgatgga ggccggtatc tggcgccaga
     1201 cgcagccatt gcgcaggcgc gtaagctgat ggccgagggg gcagatgtga tcgacctcgg
     1261 tccggcatcc agcaatcccg acgccgcgcc tgtttcgtcc gacacagaaa tcgcgcgtat
     1321 cgcgccggtg ctggacgcgc tcaaggcaga tggcattccc gtctcgctcg acagttatca
     1381 acccgcgacg caagcctatg ccttgtcgcg tggtgtggcc tatctcaatg atattcgcgg
     1441 ttttccagac gctgcgttct atccgcaatt ggcgaaatca tctgccaaac tcgtcgttat
     1501 gcattcggtg caagacgggc aggcagatcg gcgcgaggca cccgctggcg acatcatgga
     1561 tcacattgcg gcgttctttg acgcgcgcat cgcggcgctg acgggtgccg gtatcaaacg
     1621 caaccgcctt gtccttgatc ccggcatggg gttttttctg ggggctgctc ccgaaacctc
     1681 gctctcggtg ctggcgcggt tcgatgaatt gcggctgcgc ttcgatttgc cggtgcttct
     1741 gtctgtttcg cgcaaatcct ttctgcgcgc gctcacaggc cgtggtccgg gggatgtcgg
     1801 ggccgcgaca ctcgctgcag agcttgccgc cgccgcaggt ggagctgact tcatccgcac
     1861 acacgagccg cgccccttgc gcgacgggct ggcggtattg gcggcgctga aagaaaccgc
     1921 aagaattcgt taactgcaca ttcgggatat ttctctatat tcgcgcttca tcagaaaact
     1981 gaaggaacct ccattgaatc gaactaatat tttttttggt gaatcgcatt ctgactggtt
     2041 gcctgtcaga ggcggagaat ctggtgattt tgtttttcga cgtggtgacg ggcatgcctt
     2101 cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct ggagagcgtg accgcctcat
     2161 ttggctcaaa ggtcgaggtg tggcttgccc cgaggtcatc aactggcagg aggaacagga
     2221 gggtgcatgc ttggtgataa cggcaattcc gggagtaccg gcggctgatc tgtctggagc
     2281 ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc acagcctatc
     2341 ggttgatcaa tgtccgtttg agcgcaggct gtcgcgaatg ttcggacgcg ccgttgatgt
     2401 ggtgtcccgc aatgccgtca atcccgactt cttaccggac gaggacaaga gtacgccgca
     2461 gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg cggctcgacc aagagcgcac
     2521 cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac
     2581 tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga
     2641 tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg
     2701 cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc
     2761 cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct
     2821 gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg
     2881 aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac
     2941 attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc
     3001 cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc
     3061 tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg
     3121 gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat
     3181 cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac
     3241 tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg
     3301 ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata
     3361 gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg
     3421 gctgacagag acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc
     3481 tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc
     3541 ctttccgcag cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc
     3601 gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg
     3661 ggaacacatc aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc
     3721 ccttcccgca aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact
     3781 gacgcatggg ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc
     3841 gccgcgcccc acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct
     3901 cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga
     3961 gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa
     4021 gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc
     4081 ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag
     4141 gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt
     4201 cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact
     4261 cccactctta cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt
     4321 ataatgttat taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca
     4381 gttatcgggt gtcggccagt gatgagacca aaggatgcag tataagatga accactacta
     4441 ttatcatggc caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta
     4501 tgaccatcat tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta
     4561 tttaactctg tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt
     4621 tataacaatg tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca
     4681 acaggttttt tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac
     4741 agtaatatgt tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca
     4801 tggcacacag ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt
     4861 gacatggcta aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag
     4921 accagtgaac tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac
     4981 aaggagggac actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt
     5041 cagcagaaac tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg
     5101 cgggaagcag aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc
     5161 cgtctggctg acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc
     5221 tccggttcag agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc
     5281 gcctgagagg ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc
     5341 cgaaaacccg ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa
     5401 gtgcatagcg cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc
     5461 ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg
     5521 cagccggaaa acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag
     5581 gatgaaagtg acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa
     5641 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5701 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5761 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5821 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5881 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5941 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     6001 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     6061 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     6121 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     6181 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gc
//
