LOCUS       NZ_LT985313             4591 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain ECOR 25 plasmid RCS92_pII, complete
            sequence.
ACCESSION   NZ_LT985313
VERSION     NZ_LT985313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588853
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900290045.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:49:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 15
            CDSs (total)                      :: 15
            Genes (coding)                    :: 14
            CDSs (with protein)               :: 14
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECOR 25"
                     /db_xref="taxon:562"
                     /plasmid="RCS92_pII"
     gene            complement(156..1055)
                     /locus_tag="GWA84_RS00030"
                     /old_locus_tag="RCS92_PII0001"
     CDS             complement(156..1055)
                     /locus_tag="GWA84_RS00030"
                     /old_locus_tag="RCS92_PII0001"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_162482842.1"
                     /translation="MTAPSYVSPLAEYVGGKLMPGFVACDFSLGFNRDEITTAAAETFI
                     RKVQMTKSSRASQRIMFVIFARRFGKIAFNKLRSEGVLAVTIANAFGNKVDESLTRLSR
                     VVQGSLSIEKHPDELLQMVKDLESVSGENGNLRGYVFELFVSSQISNFYGLGNIYINRE
                     YKINGKHAEADVVLESSDDIYIIECKNVKVLPSTELTRWMKERVPIINSYYKINNLEKK
                     NIHHYLWVTGNVYNKDLKRLDSFKNNNKNIDVDYLFGEHLDDFFYKKKRVFNLYRKVIS
                     PDYKKGVMPDFELEDDFF"
                     /name="NERD domain-containing protein"
     gene            complement(1097..2304)
                     /locus_tag="GWA84_RS00035"
                     /old_locus_tag="RCS92_PII0002"
     CDS             complement(join(1097..1983,1983..2304))
                     /locus_tag="GWA84_RS00035"
                     /old_locus_tag="RCS92_PII0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc48 family transposase"
                     /protein_id="WP_085948175.1"
                     /translation="MAGVLLGKEVRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQPS
                     LLFKWKKQYQEGSLTAVAAGEDVVPASELAAAIKQINQLQRLLGKKTMEVEILKEAVEY
                     AQSPKMDSACALVTFGQGIAPVSRVLGVSRAQLTLRMKASDNKPDKRRQRRDEAADAEV
                     LSRILDIIGDMPAYGYRRVWAILRRQSRNEGLPFVNAKRVYRIMSENSLLLLHDKPSRR
                     QREHKGRISVKESDQRWCSDGFEFGCDDGEKLRVTFVLDCCDREAIDWAASTGGYDKAT
                     VQDVMLGAIEKRFGDKVPEQSIQWLTDNGSAYRAHETRQFARELNLKPCTTAISSPQSN
                     GMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPREYRRQRV
                     MLT"
                     /name="IS3-like element ISEc48 family transposase"
     gene            complement(2380..3039)
                     /locus_tag="GWA84_RS00040"
                     /old_locus_tag="RCS92_PII0004"
     CDS             complement(2380..3039)
                     /locus_tag="GWA84_RS00040"
                     /old_locus_tag="RCS92_PII0004"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249939230.1"
                     /translation="MKKSVVRQVLEMSGPCISSDLAERIQWQYPSMSPEAIRKMISRST
                     DIGKLPFLKFSHNRRFIYLKDDFGSFKFWRALEKCMYEANSTYSHAILAVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYETVLRNLLSAKILRAVYIDGVGDCVLINNNIANDVNIRTM
                     ANCESFFDKPIFELVKAWLRNLGLVAFNQIKTKYDGEGNPVVGSFEWDMTAPELAP"
                     /name="hypothetical protein"
     gene            4400..4558
                     /locus_tag="GWA84_RS00095"
     CDS             4400..4558
                     /locus_tag="GWA84_RS00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001403044.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHDKTPLAAAIGIWFTERRGV
                     YKHTQSS"
                     /name="hypothetical protein"
     Typing          4128..4558
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagatttggt cactgcgctc cgtttaagcc agttaccacg gttcagaaac ttgccggttt
       61 cctgaacctt cgaaaaacca ccttgccggg tggttttttc gtttcagaga tggaagatac
      121 gcctttcaaa gcgccatcgt caagaaacgc agtagctaga aaaaatcgtc ctctaactca
      181 aaatcaggca ttacgccttt tttataatca ggtgaaatga cttttctata taagttgaac
      241 acccgctttt tcttgtaaaa gaaatcatcc aaatgctcac caaacaaata atcaacatcg
      301 atattcttat tattattctt aaaagagtct aaccttttta aatctttgtt gtacacattc
      361 cctgtaaccc acaaataatg gtggatattc tttttttcta aattatttat tttatagtaa
      421 gagtttataa ttggaaccct ttctttcatc caacgcgtaa gttcagttga aggtaagacc
      481 ttaacatttt tgcactctat tatataaata tcgtcactac tctccaatac aacatcagct
      541 tctgcatgct taccatttat cttatactct ctattaatgt aaatattacc aaggccataa
      601 aaattagaaa tttgcgagga tacaaaaagc tcaaaaacat accctctcaa attgccattc
      661 tctccagaaa cagactccag atctttaacc atctgaagca actcatctgg atgcttttca
      721 atggacaaag aaccctgaac caccctcgac aacctggtta agctttcatc aactttatta
      781 ccaaacgcat ttgctatagt tacagccaga accccctcac ttctcaactt attgaaggct
      841 attttgccga aacgccttgc gaaaataaca aacattatcc tctgactcgc tcgagaagac
      901 ttggtcatct ggacttttct tatgaatgtt tctgctgcag cggtagtaat ttcatcacga
      961 ttaaagccga gagaaaaatc acaggcaacg aaaccaggca tcagttttcc gccgacatat
     1021 tcagcgagag gggaaacgta acttggagcg gtcatatcct gttcttgccc ccttattttc
     1081 agacaggggt tgtatcttaa gttaacatta cccgctgacg tcgatattct cgcggagagc
     1141 gataacccaa cgcactatgc ggatggtttt cattgtaatg ctcgatcgcc actgcaaggt
     1201 tatgcaatgc cgttcttaca tttggtttcg gcatgaacgc gatgtagtct tccttcatcg
     1261 ttttcacgaa ccgttctgcc attccattac tctgggggct actgattgcc gttgtgcagg
     1321 gcttcagatt cagctctctg gcgaactgcc gtgtttcatg cgctctgtac gctgaaccgt
     1381 tatctgtcag ccactggatg gactgttccg gtactttatc gccaaagcgt ttttctatcg
     1441 ctcctaacat cacatcctgc actgtcgctt tatcgtaacc tcccgtgctt gcggcccagt
     1501 ctatggcttc acgatcgcaa cagtccagga cgaacgtaac acgcagtttt tcgccatcat
     1561 cgcaaccgaa ttcaaagcca tcagaacacc aacgctggtc gctttccttc accgatattc
     1621 ggcctttatg ttcccgctgc cgtcgtgatg gtttgtcatg taacaacaac aggctatttt
     1681 cgctcattat ccggtaaacg cgctttgcat tcacgaacgg tagtccttca tttcgggact
     1741 gccgcctgag tatcgcccag acacggcgat acccgtatgc tggcatatcg ccaatgatat
     1801 ccaggatgcg cgataagact tctgcatccg cagcctcatc gcgcctctga cgccgtttat
     1861 ctggcttgtt atcagatgcc ttcatcctta atgttaactg cgcacgtgac acgcccagaa
     1921 cacggcttac cggggctatt ccttgtccga aggtaacaag ggcacatgcg ctatccattt
     1981 tcgcgattgt gcatactcca ccgcttcttt gaggatctca acttccatgg ttttcttacc
     2041 cagtaaacgc tgaagctgat taatctgctt tattgcagcg gccagttcag aagcaggaac
     2101 aacgtcttca ccggcagcca cagcagtgag gcttccttcc tgatattgtt tcttccactt
     2161 aaacaacaga ctgggctgaa ttccatgcaa acgcgccacg tgggacaccg tcatgcccgg
     2221 ctccatagtc tgttgaataa ttgcgatttt ttcctgaggt gttttacgtt tacgcacctc
     2281 ttttcctaac aaaacgccag ccatctcaaa attagaacta gtgttagaca tatatccaag
     2341 cctatctctt attttaagat acatctactg tctggtattt cagggggcca gctcaggagc
     2401 ggtcatatcc cattcaaaag agcctacaac tggatttccc tccccatcat attttgtttt
     2461 tatctgatta aaagcgacta ggcctaaatt cctcaaccaa gctttaacaa gctcaaatat
     2521 tggtttgtca aaaaaggatt cgcaatttgc cattgttcta atattaacat cgttggctat
     2581 gttgttattt atcagtacgc aatcaccaac tccatcaata tacacggctc taagtatttt
     2641 tgccgaaagt agatttctta gaactgtttc ataacttaaa tgtttagctt gttttatagg
     2701 agatccagac acaatcccaa aatctttcac cttcaaataa ccgccattat ttattacggc
     2761 aagtatcgcg tgtgagtaag ttgagtttgc ttcgtacatg cacttttcga gagctcgcca
     2821 aaatttgaaa ctgccaaaat catcttttag ataaatgaat ctcctgttat ggctgaattt
     2881 caaaaatggc agcttaccta tgtccgtgct tcgcgagatc attttacgta tcgcttcagg
     2941 ggacattgag ggatattgcc actgaattct ctctgcaaga tccgaagaga tgcatggccc
     3001 gctcatttca agcacctgtc taacaacact ttttttcata tcctcacctt atttctgtca
     3061 cactacgcta tttataatat tacactacgt gtaatagggc gcagtctttt aaaaaaaagt
     3121 gactataaaa aataattaaa aatcagatgg ttaattactt actttaaatt gagcacaaag
     3181 aaatatcgtc tttataatta tttaatcctt ttaaatcata aagatagtat tgtgacattt
     3241 ttttatcgaa ctatcagcta ctccatttac cataacatcc gttgcccgca ccatctcaga
     3301 cgccctcaga gcatttctgc gccctcctgc ctatttccgg catctttccc tgtccatatc
     3361 gtgaaaaaac gcgcctgatg atttcttcgc gtctgcatgg ctatgcatac gaacgcatgg
     3421 acgtcatttt agcggctaaa atccggcgag cgccctgaaa gccaccacga accccaccga
     3481 taacggtgaa agggtgcgca gtttactgtg cgaccgtgag gcgttggcca ggccgtcagg
     3541 catggcagga tttattgcta agggggtcgg atttaacctg gattaatcag cacacagtga
     3601 tgaacgggat tcggaagggg cacccttgcg gcgcggcgat gtatacatcg ctaacgtgcc
     3661 ttgtttatgg agcgcaaaaa gagagtggta gcagtccgat agcaaaaaag tagcaaaaag
     3721 atagcaaaag gagagcaacc gaaaggctca aaggcccctg aaatagcaaa aagatagcaa
     3781 aaaaatagca aaaaagtagc aaacatacgg tgtcgggcgg aacctgacca ggagtcattg
     3841 gcgtgccggc ggtactccaa aaatacatct tgtcccgctg tcgtgtagcc acaaaacatt
     3901 tcctgtccgc tgctttaacc tttgtaatcc aggaacgtca ggtgacgtca ctttgcggcg
     3961 ttcgccctat cctgcatcgt attaaaaaat gcagctatgg cgcgtacggt gccaaagaga
     4021 agcccgcaga tattccgctt cctcgctcac tgactcgctc cgctcggtcg ttcggctgcg
     4081 gcgagcggag ggaacagaga cattaagcca taggcaatga gagagaatgg agaagcaaca
     4141 agaaaggccg cggcgttgcc ggttttccat aggctccgcc cccctgacaa gcatcacaga
     4201 atctgacgct caaatcagtg gtggcgaacc ccgacaggac tataaagatc ccaggcgttt
     4261 ccccctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcact
     4321 ccgctgttat ggccgcgttt atctcattcc acgcctgaca ctcagttccg ggaaggcagt
     4381 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgcctgttc
     4441 cggtaactat cgacttgagt ccaacccgga aagacacgac aaaacgccac tggcagcagc
     4501 cattggtatc tggtttacgg agcggagagg cgtgtacaag catacacaga gttcttgaag
     4561 tgttgacctg actacggcta cactggaagg a
//
