LOCUS       NZ_LT985312             3174 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain ECOR 25 plasmid RCS92_pIII, complete
            sequence.
ACCESSION   NZ_LT985312
VERSION     NZ_LT985312.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588853
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900290045.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:49:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 15
            CDSs (total)                      :: 15
            Genes (coding)                    :: 14
            CDSs (with protein)               :: 14
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985312.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECOR 25"
                     /db_xref="taxon:562"
                     /plasmid="RCS92_pIII"
     gene            complement(202..1311)
                     /gene="wzz(fepE)"
                     /locus_tag="GWA84_RS00005"
                     /old_locus_tag="RCS92_PIII0001"
     CDS             complement(202..1311)
                     /gene="wzz(fepE)"
                     /locus_tag="GWA84_RS00005"
                     /old_locus_tag="RCS92_PIII0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_053890740.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEGIDLFGLISVLWAAKIKIVGVIL
                     FFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDNAFDLF
                     IKKFQSVNSLNEYMRSSPYVMEMIKKNNISALELHRAIVELSKNMKSVDDNTSKKNDKS
                     SLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEILALD
                     EIKMRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATKLNI
                     KKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAIIVI
                     LSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            2157..2351
                     /locus_tag="GWA84_RS00010"
                     /old_locus_tag="RCS92_PIII0002"
     CDS             2157..2351
                     /locus_tag="GWA84_RS00010"
                     /old_locus_tag="RCS92_PIII0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041630.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAENETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2997..3131
                     /locus_tag="GWA84_RS00090"
     CDS             2997..3131
                     /locus_tag="GWA84_RS00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071606675.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY"
                     /name="replication initiation protein"
     Typing          complement(1643..1891)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="86.0"
                     /evalue="4.400000000000001e-111"
     Typing          complement(2023..2121)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.0"
                     /coverage="100.0"
                     /evalue="6.26e-40"
     Typing          complement(2432..2518)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.455"
                     /coverage="100.0"
                     /evalue="1.14e-31"
     Typing          2543..3069
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.956"
                     /coverage="98.0"
                     /evalue="2.2599999999999997e-176"
ORIGIN
        1 cagatttggt gacatgtctc gcacaagact gttaccacgg ttcagaagtt ccccaactta
       61 ctgaaccttc gaaaaaccac ctccccaggt ggttttttcg ttttctagag tagaagacta
      121 cggctgtaag ggaatgatct caagaagatc ctttgaactt tttataaaag ttgcttataa
      181 aatgatgcat cagcacaact tttactcaat cacactcatc tttctggcct ccatggcatg
      241 acgtaaaagt atatagccac atgcaagcat ccctccaatg acagaagaaa gaattacaat
      301 tatggcatta cttggcccat cctttctgac cggtaacgtt gggctcaatt gatactgaaa
      361 cggcataaaa tcaacatccc ccactttcgc cacaaccaat tgattcacaa catattgacg
      421 atttcgcaac tccccactca attccgaaac atccttgatt gattttttga tgttcaattt
      481 agttgctata ccatcagaac cgagagccac aggaaaatct gggtcatcct tcataatctg
      541 accattgctg tatacaggtt tttttattcc agccgcatta gcaacctcca gtgaataatt
      601 gaggcgtcga atatctgcat tcagctggtt tctcattttt atctcatcca gtgcaagaat
      661 ttctttttca acattagttt taacttctag tttatttctt atatcttcca tcaaatccct
      721 tacaacaagg gaagaaacat aattaatata cccagacaac acatcgtgag cttctttact
      781 tgttggagca gtaaaactca gagtccatga tacatacaat gatgacttgt cgtttttctt
      841 actggtatta tcatcaaccg acttcatatt tttactcaac tcaacaattg cacgatgcag
      901 ttcgagagcg ctaatgttat tctttttaat catctccata acataaggag atgatctcat
      961 atactcattc aacgaattaa cggactgaaa tttcttaata aacagatcaa atgcgttatc
     1021 acgattgata tcaaaatcca ctcccagaac atgaagctta gaaagctcct tatttaattc
     1081 actccattga acagactcag caggagtaac cacagctgaa ctagtccatt tttgtggcaa
     1141 aacaaaagaa acaagaaagc caatacaagc aaaaaataga atcaccccaa caatttttat
     1201 ttttgctgcc cataaaacgc taatcaagcc aaaaagatca attccttcat taccattcgg
     1261 caacatgttg taattagaaa aatgttcgtc agataccttt gagattttca ttaaaaaaaa
     1321 acacaatata ttaacatatg gttttttatt ctatcatatg ttaggaaaaa acctaaaaac
     1381 cattcagcag acatttaaca taacatttta ttacgcgcac cgctaaaacg cgctgaacgc
     1441 gattcagccg caaacaccgc aaaaagatat gttttccccg gggtaaaccg gaaaaatgcg
     1501 tcaggatcgc tttcagctca ctgcatagct atgcatgaaa gtgaatgacg atcggtttgg
     1561 gcgcatacag tgcccatgag acacattttc ggacattttc agttcgtgaa tccctgactc
     1621 attgctacat atctgaacag acacccctag cagcgccccc aggggcgtcc tgtaaaaaaa
     1681 cacaccgcgc cgctagcagc actcctaata taagatgatg ttttttatga aaatagtcag
     1741 taccacccct aaaaagcggt gttggcgccg ctacaacacc gcgccgacac cgctttttta
     1801 aatatcacaa agagagcaag aagaactaat ttgtcataac tctctattta taaagaaaaa
     1861 tcagcaaaaa attgtttttg cgtggggtgt gagcatttca ggcatgagaa cagactcgcg
     1921 tttttccgga gtatcagcgg aattattgat taagaaacac tcgacgcaga gcgaaatttt
     1981 atgcaacagc ggctgcactg cacagggatt atttatcagt tgatatgctg tgccatgata
     2041 cgtcctaaag aaacaggaca ggatgtgcaa tcgtaatacc gtcgcccgca acgtcttaca
     2101 attaccacct gctcagggtc cccccgacac ccggaactga cggagaacaa cggaaggtga
     2161 acaaacaaca acaaactgcg ctgaatatgg cgcgatttat caaaagccag agcctgacac
     2221 tgcttgaaaa actggatgct ctggatgccg acgaacaggc ggccatgtgc gaacgactgc
     2281 acgaactcgc agaagaactc cagaacagca tccagattcg ctttgaggcc gaaaatgaaa
     2341 caggaacata acgaagctga cggaggcggt cacggctttc ccgtgggcgt tttcgtcagc
     2401 agacaagccc gtcagggcgc gtcagggggt tttagcgggt gtcggggcgc agccatgacc
     2461 cagtcacgta gcgatagcgg agtgtatact agcttaatta tgcggcgcca gagcagatgg
     2521 tgagagaaca gcgacatatg aggtgtgaaa tgctgcacag atacgtaagg agaaaatgca
     2581 gcttccggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2641 ggcgagcggt gtctgctcac gcaaagacgg atatacggtt atccacagaa tcaggggata
     2701 acaccggaaa aaacatgtga gcaaaaaaga ggccccgaaa aaaggccgcg ccggtggcgt
     2761 ttttccatag gctccgcccc cctgacaagc atcacaaaaa tcgacgctca agtcagaggt
     2821 ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct ccctcgtgcg
     2881 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgttcgtc
     2941 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg ggctgtgtgc
     3001 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3061 acccggtaag acacgactta acgccattgg cagcggccac tggtaaacag gatgtgcgag
     3121 agatgtattg agagttcttg aagtggcagc cttactgagg ctacactaga agga
//
