LOCUS       NZ_LT985308             4913 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain CIP106223 plasmid RCS93_pIV, complete
            sequence.
ACCESSION   NZ_LT985308
VERSION     NZ_LT985308.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588852
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900290015.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 04:04:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 153
            CDSs (total)                      :: 153
            Genes (coding)                    :: 135
            CDSs (with protein)               :: 135
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 10 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 3 of 18
            Pseudo Genes (multiple problems)  :: 8 of 18
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985308.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CIP106223"
                     /db_xref="taxon:562"
                     /plasmid="RCS93_pIV"
     gene            607..834
                     /locus_tag="GWB16_RS00010"
                     /old_locus_tag="RCS93_PIV0001"
     CDS             607..834
                     /locus_tag="GWB16_RS00010"
                     /old_locus_tag="RCS93_PIV0001"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_015056439.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(831..2396)
                     /locus_tag="GWB16_RS00015"
                     /old_locus_tag="RCS93_PIV0002"
     CDS             complement(831..2396)
                     /locus_tag="GWB16_RS00015"
                     /old_locus_tag="RCS93_PIV0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_087934827.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            2656..3093
                     /locus_tag="GWB16_RS00020"
                     /old_locus_tag="RCS93_PIV0003"
     CDS             2656..3093
                     /locus_tag="GWB16_RS00020"
                     /old_locus_tag="RCS93_PIV0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            3174..3593
                     /locus_tag="GWB16_RS00025"
                     /old_locus_tag="RCS93_PIV0004"
     CDS             3174..3593
                     /locus_tag="GWB16_RS00025"
                     /old_locus_tag="RCS93_PIV0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3818..4141)
                     /gene="mobA"
                     /locus_tag="GWB16_RS00030"
                     /old_locus_tag="RCS93_PIV0005"
     CDS             complement(3818..4141)
                     /gene="mobA"
                     /locus_tag="GWB16_RS00030"
                     /old_locus_tag="RCS93_PIV0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            4425..4619
                     /locus_tag="GWB16_RS00035"
                     /old_locus_tag="RCS93_PIV0006"
     CDS             4425..4619
                     /locus_tag="GWB16_RS00035"
                     /old_locus_tag="RCS93_PIV0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(4151..4390)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(3822..4141)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.11e-62"
ORIGIN
        1 tatcgtcttg agtccaaccc ggtaagacac gacttagcgc cactggcagc agccgttggt
       61 aacttggtta gcagagcgat gatggcgcaa atggtgcaac agagttcttg aagtagtggc
      121 ccgactacgg ctacactgga aggacagtat ttggtatctg cgctctgctg aagccagtta
      181 cctcggttaa gcagttcccc aactgactta accttcgatc aaaccgcctc cccaggcggt
      241 tttttcgttt acaggcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      301 atcttttcta ctgaacagag atccccgtca atttagaaga agatgatgga gccacagtcc
      361 ctccctgaac atcacgtata tagttagcct gacatccaac aaggaggttt atagcgaata
      421 ttcccacaaa aaatcttttc ctcataactc gatccttata aaatgaaaag aatatataac
      481 gaggtttaat ttatgagttt aagatactac ataaaaaata ttttgtttgg cctatactgc
      541 gcacttatat atatatacct tataacaaaa aacaacgaag ggtattattt cctagcgtca
      601 gataagatgc tatacgcaat agtgataagc actattctat gcccatattc aaaatatgct
      661 attgaacaca tattttttaa gttcataaag aaagattttt tcagaaaaag aaaaaaccta
      721 aataatgccc ccgtggcaaa attaaaccta tttatgctat acaatctact ttgtttggtc
      781 ctagcaatcc catttggatt gctaggactg tttatatcaa taaagaataa ttaaataccc
      841 aacacctcat ttatagtatt aagtttattc ttatcaataa aggcacatag aatgcctgta
      901 acaatagcaa tcccccagat acctaatgta gttccagcaa gcacactaaa aagtaaaaca
      961 acaatataac taactccagc atctacagct ttcttctcta atgtaagaaa tagcggcttc
     1021 cagtcacctg tatccttaat ttttaggata tcagatacca catcatatcc aaaagaaaca
     1081 tgtcccgtaa tcttcaagta cttggcaaac tgatctaaat gcttagccca gtcttcatac
     1141 ttaaccgcct ccagtgcatt gaaaattgca tcacggtctg ctttgctgaa tttcttattt
     1201 aatacatcct tgtatttctc aaaagcagct agagcttcat tcacattgct gattttctta
     1261 cctttagatt tatcagcaag ttcctgtgcc atttttgaat atttttcacc atatttttca
     1321 gatagcgttt gataaaagct aactgttgca tccacagcat ccttaatctg cgagttcagg
     1381 agattagtct gtgctgtttt caaattatct tcagcatcat gaatacgtga aactgctgta
     1441 cttcgcttat tattagcctg agaaatagcc tcttggattc catttatctc agagctaatc
     1501 tggttaagac gcgtttctga tgctgttacc tgcttttgtt tttcctccat ctcatcacca
     1561 gctctcgccc gtagtctggt ggcctcaaaa aaaggacgac tctgaagcgg atcattcgcc
     1621 ctaggagata aaagtttaac cctttcatcc agttctttat atttagcaga tgcctgatcc
     1681 aactcatttc gttttccgga cagcgtattc gtttctgcat cacgggcatg aatactggag
     1741 cttaaacggg tattgagcgt attaatctct tcatccactt tcgccagctc agattgtgca
     1801 gcagcaagat ttttttgtgc cacctccaca gcccgggcct cttcactcaa tgctgccagg
     1861 cgtttctctt catcctcagc cagtttcaat tggcgttctg tttcagcctg ctccttctct
     1921 atctctttac gtcgttgttc tgcttcctga aaagcctttt ccgcagcttc cgcttcttta
     1981 cgggcttttt cttctgcttt tgcaaggcgc aaccgctctg cttctgcctg catcgctgca
     2041 ttattggcat gagccaggtc aataacctcc ggatgagtgg aattatggcg aagcgcctca
     2101 ttcacaatat ccttcagatg ttgagtcagc gcatcccggt ttgcttttgc tttagcctgt
     2161 gcttctgcgg cagcttttgc tcgggcagcc tgttctgcct gcgttttctt caattgagca
     2221 gtagaccatt tagctgtggc atgaatggct gcagaacttt cacttttgct acctccagtt
     2281 ccaccaccac cgccagagcc actcccgtct ggattaccat tcaaaagagt aatgattacc
     2341 tgccccttat catcataagg aacaccatct ttatagtacg ctacagctgt ttccattata
     2401 aaatcctctt tgacattaaa aacaaatagt taaaagtaat atactgtata tataatcact
     2461 ggttttatat acagtttaaa attacgccgc tgcattttcc ctgtcaaccc tgtggatttt
     2521 catttttgtg aaaacgatca aaaaaacagc cacccacacc acgtgctatt tccacccgat
     2581 gccacaaaaa ccagcacaaa cattaccgtt ctcagacctc attatgtttt actgaaacca
     2641 tgagatgaga cacctatggg acactgtaac tttatggcat ggcacacact ccgggacgca
     2701 ctgaaaatga ctggaagatc gcgttcacag ttttaccgtg atatgcgctc aggtcttgtc
     2761 agttaccgta ccagccagga cggacgacgg gagtttgaaa ccagtgaact gatccgggca
     2821 tacggcgaat taaagcggaa tgagacacca gaaaggcaca ctcaaggaca ctcagaaaaa
     2881 cctcatgatc agcagacaga acgcattctc cgggaactga atgagctgaa acaatgcctg
     2941 acgctgatgc ttgaggataa acaagcccag gatacggagc gccggcgcca ggaagcagaa
     3001 cggaaacagc tacagaatga gatagcccag ctcaggcagg ctctggagca ggaaaagaaa
     3061 cggggattct ggtccaggct gttcggtcac tgaacgcgct cagaaactga tgataagata
     3121 accttcggat aataactcac cgagaataaa tactttaagg tagggagaca ctcatgagac
     3181 gtaccggaaa caaactttgt cttatcgcca tgataacagc aacagtagct ctcacagcct
     3241 gtaccccaaa gggcagcgtg gaacaacata cccggcatta cgtatatgct tctgatgacg
     3301 gttttgatcc caacttttcc acccaaaaag ccgacacaac acgaatgatg gtgccttttt
     3361 ttcggcagtt ctgggatatg ggagctaaag ataaagcgac aggaaaatca cggagtgatg
     3421 tgcaacaacg cattcaacag tttcacagcc aggaattttt aaactcactc cggggcacaa
     3481 ctcaatttgc gggtactgat taccgcagca aagaccttac cccgaaaaaa tccaggctgc
     3541 tggctgacac gatttctgcg gtttatctcg atggctacga gggcagacag taagtggatt
     3601 taccataatc ccttaattgt acgcaccact gaaacgcgct gaacgcgatt ctgacgcaga
     3661 caccgcaaaa agagatgttt taccccggaa aaacaggaag gatgcgtcag gatcgttttc
     3721 agctctctgc atagctatgc atgaaagcga atggtgatca ctttgggagc ttacggtgtt
     3781 cataccgtca gttttcgaca gttttctctc cgggaagcta atctgccata agcctggata
     3841 acagggcacg gtgatactcc gtaatagcga tcagcacctc cgcatactcc ctgtccccga
     3901 cacgcttgcc gtcgataaag agttttttct gtaacgcccc cagtctcata acttctttca
     3961 gtacccgatc atcagtcagt gagttaactt tcttaccgag agctgccgcc cgtaaaaaac
     4021 cggaaacggt cttgccgcat tcagcagccc tttttctgat ttcctggtct tcttctgccg
     4081 tcagacgaac agggcgactg atagcccgcc tgcgcgtatt acttccactt cgttttgtca
     4141 taaaacatgg ctccgtatct gacatgggtg tcggggcaaa gccctgacca gggtaattgt
     4201 aatagcgtgc atgtatgcgc ggtataacaa ttacacatcc tgtcctgtca gcaagttcga
     4261 attaccgaca aaggcacggc agttttttgg cagtgacagt attgtcaggc agaaataccc
     4321 gtcaaagcgt cagacggcag aataccggcg gtaaaacagt cgaaaagtgg tacgcgatat
     4381 aatcacacaa cgcagatgac agacagaacg gagaaaacga aaagatgaac aagcagcagc
     4441 aagccgtact caacatggca ggattcataa aaagccaaag cctgacgctg ctcgaaaagc
     4501 tggacgcact cgatgctgac gaacaggcta ccatgtgtga gaagctgcac gaactcgcag
     4561 aagaactcca gaacagcatc cagacacgct ttgaagtaga aaatagcaca gagagataac
     4621 gctcaggaca ggatgccgaa cggcacaaca gagcgtaagg agaaaatgtc acataaggcg
     4681 ctcttccgct tcctcgctca ctgactcgct acgctcggac gttcgactgc ggcgagcggt
     4741 gtcagctcac tcaaaagcgg taatacggtt atccacagaa tcaggggata aagccggaaa
     4801 gaacatgtga gcaaaaagca aagcaccgga agaagccagc gccgcaggcg tttttccata
     4861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagccagagg tgg
//
