LOCUS       NZ_LT985307             6069 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain ECOR 4 plasmid RCS86_pI, complete sequence.
ACCESSION   NZ_LT985307
VERSION     NZ_LT985307.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588809
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289995.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 00:59:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985307.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECOR 4"
                     /db_xref="taxon:562"
                     /plasmid="RCS86_pI"
     gene            447..605
                     /locus_tag="GWA52_RS00045"
     CDS             447..605
                     /locus_tag="GWA52_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1065..1880
                     /gene="sul2"
                     /locus_tag="GWA52_RS00010"
                     /old_locus_tag="RCS86_PI0001"
     ARG             1066..1877
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1967..>2209
                     /gene="glmM"
                     /locus_tag="GWA52_RS00015"
                     /old_locus_tag="RCS86_PICDS1967D"
                     /pseudo=""
     CDS             1967..>2209
                     /gene="glmM"
                     /locus_tag="GWA52_RS00015"
                     /old_locus_tag="RCS86_PICDS1967D"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(2445..3938)
                     /locus_tag="GWA52_RS00025"
                     /old_locus_tag="RCS86_PI0002"
     CDS             complement(2445..3938)
                     /locus_tag="GWA52_RS00025"
                     /old_locus_tag="RCS86_PI0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_001120888.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            complement(3982..4404)
                     /locus_tag="GWA52_RS00030"
                     /old_locus_tag="RCS86_PI0003"
     CDS             complement(3982..4404)
                     /locus_tag="GWA52_RS00030"
                     /old_locus_tag="RCS86_PI0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4589..5029
                     /locus_tag="GWA52_RS00035"
                     /old_locus_tag="RCS86_PI0004"
     CDS             4589..5029
                     /locus_tag="GWA52_RS00035"
                     /old_locus_tag="RCS86_PI0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(5796..5945)
                     /locus_tag="GWA52_RS00040"
                     /old_locus_tag="RCS86_PI0005"
     CDS             complement(5796..5945)
                     /locus_tag="GWA52_RS00040"
                     /old_locus_tag="RCS86_PI0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          complement(2..85)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.75e-36"
     Typing          complement(5981..6069)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="2.12e-37"
     Typing          9..614
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg tcagcagaat atgcgataca
       61 ggagatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag
      121 cggtaccggc ttacggacgg ggacggaaat ttcctggaag ataccaggaa gacacttagc
      181 aaagaagaga taaggccacg gcgaagccgt ttttccatag gctccgcccc cctgacaagc
      241 atcgcaaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatccc
      301 aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
      361 gtgtcattcc gctgttatgg ccgcggtttc tcattccacg cctgacactc agttccgtga
      421 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
      481 ccttgtccgg taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg
      541 cagcagccat tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg
      601 cctgactgcg gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta
      661 ccgggttaag cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt
      721 ttttcgttta cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg
      781 tagattacta tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac
      841 cgctccgtaa caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat
      901 aaatatatct acttattgag agatgatata tttatttcca aaatgctaca gatacatcat
      961 catgaaacgg atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat
     1021 ccgtatgaga ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca
     1081 tcattttcgg catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg
     1141 cgccagacgc agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg
     1201 acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg
     1261 cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca
     1321 gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata
     1381 ttcgcggttt tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg
     1441 tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca
     1501 tcatggatca cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta
     1561 tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg
     1621 aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg
     1681 tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg
     1741 atgtcggggc cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca
     1801 tccgcacaca cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag
     1861 aaaccgcaag aattcgttaa ctgcacattc gggatatttc tctatattcg cggttcagca
     1921 ggcatgtccc ctttgagggc gacccgacga caggataatc gaccttatgg tgcgcaaata
     1981 tttcggcaca gacggtattc gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc
     2041 cttgcgcgtc ggcatggcgg ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt
     2101 cgtgatcggc aaggatacgc gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg
     2161 tttcacctcg atgggcatgg acgtattcct ttttggcccg ctgccgacaa cgtataggaa
     2221 gaataaacgc ccttttcacc caagtccaac agctttggac cgcagttgac tctttcgaca
     2281 cccctgcgat gcaacccaat ccggctgacg gggagccagc aacgctgaaa atttaccctc
     2341 ctctttccca ctagcggctc cttttccgac aaccagcacg gcggatccct gccgcggcgc
     2401 tgtgaacgca gcattttgat tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc
     2461 aggagcagct cagccggtgg cgcccggctt tcgggtaacg ccctggtccc gctggtttcg
     2521 gctttgtgct tcaggtgatc aaggatctgc ttgatcacta tagggtcttc aatgcaggcg
     2581 atgactttca tggcgccgcc gcagccgctg caggtctcga tgtcgatatt gaaaacacgc
     2641 ttgagccgtt gcgcccatgt catcgacgct cgccgttgtg ctggtgttgc cggttcatca
     2701 gccaccctga ccttgttgcc cctgccccgt tttgccggcg tgaccaacgc ccggtgccga
     2761 ctgttgggtg cgaacacccc gtggaagcgg gttaggttga ctctgggctt cggtaccagg
     2821 gcggccagcc ttgcaatgaa atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg
     2881 tacggcgtct tgagctggta gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg
     2941 gataccgccg ggcggctgat gtaccggcac agccgttcga gcttcttgcg ttcatcggcc
     3001 ctggccgcca cgccggcgtg caggctggac ccggctacct tgccaatccc gtcaccgaac
     3061 ggatcaccac tggtcggcag agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg
     3121 acagcgatac ggtaagtgat cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc
     3181 gcatccgagg ccagatagct gttttcgaca tcccgttcca gcaggccttg ccgttccaga
     3241 tagcgaccca cccggtgggc gatggtgtgc gtcagctggg tgagctctgg gctggtcggc
     3301 gccttgaccc agcggaaacg cgctgagccg tgggattgct cgacatacac accgtcgaga
     3361 aacagcatgt ggaagtgaac attcagattg agcgccgatc caaaacgctg gatcagggtg
     3421 accgcgcccg tcttggccac ttggtgggta tggcccgctt tcttgaccag gtgcgtggca
     3481 atgacgcggt aaacgatgcc cagcacccac cccatgatct cgggccggct ggcaaacagg
     3541 aaacgcagct gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact
     3601 tcatcaacca gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa
     3661 ccgcgacgct tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc
     3721 cgtagaaagc catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca
     3781 tagcccggca attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg
     3841 tcaacgatct gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc
     3901 cgatcccggc tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg
     3961 tgtttacata cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc
     4021 cgttgctaga cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc
     4081 taggtacatg ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag
     4141 atcgacagaa ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag
     4201 accaaaggat gcagtataag atgaaccact actattatca tggccaccat taccaccacc
     4261 gttaaaacta cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg
     4321 tcccatgtga ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc
     4381 ctctttgact tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat
     4441 tcgaaatact acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc
     4501 ctcacgggca gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac
     4561 ggtacacaca ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa
     4621 attaacagga aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta
     4681 tcgcacagga aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg
     4741 cgagctgcgc acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt
     4801 tagccaggcg gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca
     4861 gactgttacc ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa
     4921 tgaggagagg gaacaactac aggccgaaat tagccgtctg gctgacgcgc tggcacagga
     4981 aaaaaagcgg ggattctggt cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg
     5041 actctgattt accataacat ctgttatcag caccgcctga gaggccctca gggcgtttct
     5101 gacgggaaac cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc
     5161 tctgttttta cggcatgcat gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat
     5221 tcagccgggg taagtcctgt ttccggcgtt cttcccatcc gaaaacggtg aacggggacg
     5281 caatttattg cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg
     5341 ttcaggaagg gggtgatgta caaatggcac agaggatgaa agtgacgtat gagctgaagg
     5401 aatgatgtaa aagtgatgta taagtgatgt aaaagtgatg tataagtgat gtaaaagtga
     5461 tgtatgaact gaaggagcga tgtaattctg tagtcaggaa gaataacccg ttcatgcagg
     5521 catgaacgat acagtgagga acgctttttt tgtcacgatc tccccttaac ctgcgtaaat
     5581 cacccgccat ggttgattat ttttcaacct caaaatggtc atacacctaa cgcagccgcg
     5641 attttcatcg caactgcatg aattaaaaga aaaaaaagga atatttcgtt ttcacgagta
     5701 atacccgggt attaaaaatc cactgtaatt acatgaaaat aaagaaataa aaggtaatac
     5761 cggagtatta ccacgtacgc gggcaagcga aaaagctata acgcttcccg gccgacaggc
     5821 gacccaaaat ccacaggggc atgcaggttc tcttcagtaa cctgcgccat taaatcaaaa
     5881 aggtcatatt cgccgggaag ctgcgtatca gtcgaatcca gttgttcatc atattcagat
     5941 ggcatggggt gatatccagt agctgagagg ttgttagcgg gtttcggggc gcagccctga
     6001 accagtcatg tagcgctagc ggagtgtata ctggcttact atgttgccac gattgcgaaa
     6061 atcagtgaa
//
