LOCUS       NZ_LT985298             6047 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain ECOR 18 plasmid RCS87_p, complete sequence.
ACCESSION   NZ_LT985298
VERSION     NZ_LT985298.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA104588808
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Cea,G.-C. and William,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2018) Genoscope CEA, 2 rue Gaston Cremieux CP5706
            91057 Evry cedex, France, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900289925.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:32:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT985298.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECOR 18"
                     /db_xref="taxon:562"
                     /plasmid="RCS87_p"
     gene            486..644
                     /locus_tag="GWA51_RS00045"
     CDS             486..644
                     /locus_tag="GWA51_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1104..1919
                     /gene="sul2"
                     /locus_tag="GWA51_RS00010"
                     /old_locus_tag="RCS87_P0001"
     ARG             1105..1916
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2006..>2248
                     /gene="glmM"
                     /locus_tag="GWA51_RS00015"
                     /old_locus_tag="RCS87_PCDS2006D"
                     /pseudo=""
     CDS             2006..>2248
                     /gene="glmM"
                     /locus_tag="GWA51_RS00015"
                     /old_locus_tag="RCS87_PCDS2006D"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(2484..3977)
                     /locus_tag="GWA51_RS00025"
                     /old_locus_tag="RCS87_P0002"
     CDS             complement(2484..3977)
                     /locus_tag="GWA51_RS00025"
                     /old_locus_tag="RCS87_P0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_001120888.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            complement(4021..4443)
                     /locus_tag="GWA51_RS00030"
                     /old_locus_tag="RCS87_P0003"
     CDS             complement(4021..4443)
                     /locus_tag="GWA51_RS00030"
                     /old_locus_tag="RCS87_P0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4628..5068
                     /locus_tag="GWA51_RS00035"
                     /old_locus_tag="RCS87_P0004"
     CDS             4628..5068
                     /locus_tag="GWA51_RS00035"
                     /old_locus_tag="RCS87_P0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(5813..5962)
                     /locus_tag="GWA51_RS00040"
                     /old_locus_tag="RCS87_P0005"
     CDS             complement(5813..5962)
                     /locus_tag="GWA51_RS00040"
                     /old_locus_tag="RCS87_P0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          complement(2..124)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.194"
                     /coverage="100.0"
                     /evalue="2.67e-56"
     Typing          48..653
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctggcttact atgttgccac gattgcgaaa atcagtgaag tgcttcatgt ggcaggagaa
       61 aaaaggcagc accggcgcgt cagcagaata tgcgatacag gagatattcc gcttcctcgc
      121 tcactgactc gctacgctcg gtcgttcggc tgcggcgagc ggtaccggct tacggacggg
      181 gacggaaatt tcctggaaga taccaggaag acacttagca aagaagagat aaggccacgg
      241 cgaagccgtt tttccatagg ctccgccccc ctgacaagca tcgcaaaatc tgacgctcaa
      301 atcagtggtg gcgaaacccg acaggactat aaagatccca ggcgtttccc cctggtagct
      361 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
      421 cgcggtttct cattccacgc ctgacactca gttccgtgaa ggcagttcgc tccaagctgg
      481 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttgtccggt aactatcaac
      541 ttgagtccaa cccggaaaga cacgacaaaa cgccactggc agcagccatt agtaacagga
      601 gtgcacgaga agatagatta agaggtcttg aagtagaggc ctgactgcgg ctacactgaa
      661 agaacagttt tggtaatggc ggtctcgtga accctgttac cgggttaagc agttccccaa
      721 ctgacttaac cttcgaacaa accgcctccc caggcggttt tttcgtttac agagcaagag
      781 attacgattc attgaaaagg atctcaagaa gatcctttgt agattactat caattaatct
      841 tccgaacatg acaccagtca ctgttgatta cggttaaacc gctccgtaac aggtttccct
      901 cgtattggtg acttgcagca ccaaagaagc aaaaaagata aatatatcta cttattgaga
      961 gatgatatat ttatttccaa aatgctacag atacatcatc atgaaacgga tcaccgcaaa
     1021 caggttactc gcctggggat tccctttcga cccgagcatc cgtatgagac tcatgctcga
     1081 ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc atcgtcaaca
     1141 taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca gccattgcgc
     1201 aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctcggtccg gcatccagca
     1261 atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg
     1321 acgcgctcaa ggcagatggc attcccgtct cgctcgacag ttatcaaccc gcgacgcaag
     1381 cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt ccagacgctg
     1441 cgttctatcc gcaattggcg aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag
     1501 acgggcaggc agatcggcgc gaggcacccg ctggcgacat catggatcac attgcggcgt
     1561 tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc
     1621 ttgatcccgg catggggttt tttctggggg ctgctcccga aacctcgctc tcggtgctgg
     1681 cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca
     1741 aatcctttct gcgcgcgctc acaggccgtg gtccggggga tgtcggggcc gcgacactcg
     1801 ctgcagagct tgccgccgcc gcaggtggag ctgacttcat ccgcacacac gagccgcgcc
     1861 ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga aaccgcaaga attcgttaac
     1921 tgcacattcg ggatatttct ctatattcgc ggttcagcag gcatgtcccc tttgagggcg
     1981 acccgacgac aggataatcg accttatggt gcgcaaatat ttcggcacag acggtattcg
     2041 tggcaaagcc aacgaaggcg cgatgacggc ggaaaccgcc ttgcgcgtcg gcatggcggc
     2101 tggccgtgtc tttcgtcgcg gtgaccaccg ccatcgtgtc gtgatcggca aggatacgcg
     2161 cctgtcgggc tatatgcttg aacccgcgct cacagccggt ttcacctcga tgggcatgga
     2221 cgtattcctt tttggcccgc tgccgacaac gtataggaag aataaacgcc cttttcaccc
     2281 aagtccaaca gctttggacc gcagttgact ctttcgacac ccctgcgatg caacccaatc
     2341 cggctgacgg ggagccagca acgctgaaaa tttaccctcc tctttcccac tagcggctcc
     2401 ttttccgaca accagcacgg cggatccctg ccgcggcgct gtgaacgcag cattttgatt
     2461 ggtatcgttg gccttcaggc tcgtcagtca aacagaccca ggagcagctc agccggtggc
     2521 gcccggcttt cgggtaacgc cctggtcccg ctggtttcgg ctttgtgctt caggtgatca
     2581 aggatctgct tgatcactat agggtcttca atgcaggcga tgactttcat ggcgccgccg
     2641 cagccgctgc aggtctcgat gtcgatattg aaaacacgct tgagccgttg cgcccatgtc
     2701 atcgacgctc gccgttgtgc tggtgttgcc ggttcatcag ccaccctgac cttgttgccc
     2761 ctgccccgtt ttgccggcgt gaccaacgcc cggtgccgac tgttgggtgc gaacaccccg
     2821 tggaagcggg ttaggttgac tctgggcttc ggtaccaggg cggccagcct tgcaatgaaa
     2881 tccaatggtt cgaaaatgac gtgcgtggtg ccgtcccggt acggcgtctt gagctggtag
     2941 cgcacgttgc cgcctcgtgt taacgacagc cgcttctcgg ataccgccgg gcggctgatg
     3001 taccggcaca gccgttcgag cttcttgcgt tcatcggccc tggccgccac gccggcgtgc
     3061 aggctggacc cggctacctt gccaatcccg tcaccgaacg gatcaccact ggtcggcaga
     3121 gtttgcaaag tgaacacctt tcgccccgcc tgtgaaccga cagcgatacg gtaagtgatc
     3181 gagtgcccca gcaggggtgt catcgggtcg tcatccaccg catccgaggc cagatagctg
     3241 ttttcgacat cccgttccag caggccttgc cgttccagat agcgacccac ccggtgggcg
     3301 atggtgtgcg tcagctgggt gagctctggg ctggtcggcg ccttgaccca gcggaaacgc
     3361 gctgagccgt gggattgctc gacatacaca ccgtcgagaa acagcatgtg gaagtgaaca
     3421 ttcagattga gcgccgatcc aaaacgctgg atcagggtga ccgcgcccgt cttggccact
     3481 tggtgggtat ggcccgcttt cttgaccagg tgcgtggcaa tgacgcggta aacgatgccc
     3541 agcacccacc ccatgatctc gggccggctg gcaaacagga aacgcagctg aaacgggaag
     3601 ctcaacaccc actgacgcat gggttgttca ggcagtactt catcaaccag caaggcggca
     3661 ctttcggcca tccgccgcgc cccacagctc gggcagaaac cgcgacgctt acagctgaaa
     3721 gcgaccaggt gctcggcgtg gcaagactcg cagcgaaccc gtagaaagcc atgctccagc
     3781 cgcccgcatt ggagaaattc ttcaaattcc cgttgcacat agcccggcaa ttcctttccc
     3841 tgctctgcca taagcgcagc gaatgccggg taatactcgt caacgatctg atagagaagg
     3901 gtttgctcgg gtcggtggct ctggtaacga ccagtatccc gatcccggct ggccgtcctg
     3961 gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt gtttacatac agtctatcgc
     4021 ttagcggaaa gttcttttac cctcagccga aatgcctgcc gttgctagac attgccagcc
     4081 agtgcccgtc actcccactc ttacattgtt cgcagtatct aggtacatgt tattgcgtgc
     4141 accttcatga tgtataatgt tattaatgac agcagaaaga tcgacagaat taatattctt
     4201 cgtacctggg acagttatcg ggtgtcggcc agtgatgaga ccaaaggatg cagtataaga
     4261 tgaaccacta ctattatcat ggccaccatt accaccaccg ttaaaactac ctcccgaact
     4321 tccagagccg ctatgaccat cattgtaggg gtcatttggt cccatgtgac caaaaccacc
     4381 gacagtaccg ctatttaact ctgtactcat aaaattatcc tctttgactt ttaaaaacag
     4441 caaaaactgt atttataaca atgtgtttat atacagtatt cgaaatacta cgcccgtcaa
     4501 aaccacaccg tcaacaggtt ttttgtgtat ttttcacgcc tcacgggcag cctcagacct
     4561 tttgtgttaa aacagtaata tgttctgctg ttacaatacg gtacacacag gagacactgt
     4621 cacttttatg gcatggcaca cagttcaaga gacactgaaa ttaacaggaa agtcgcgctc
     4681 gcaattatat cgtgacatgg ctaaaggcct tgtcagctat cgcacaggaa aaaatgatcg
     4741 ccgggagttc gagaccagtg aactgattcg ggcctatggc gagctgcgca cttctgagac
     4801 accaaaaagg cacaaggagg gacactccaa atctgtcgtt agccaggcgg aaaatgctca
     4861 gctagacgtc attcagcaga aactggacgc tctgcagcag actgttaccc taatgcttga
     4921 agacaagatg gcgcgggaag cagaaaaccg cctgcgaaat gaggagaggg aacaactaca
     4981 ggccgaaatt agccgtctgg ctgacgcgct ggcacaggaa aaaaagcggg gattctggtc
     5041 gcggctgttt cgctccggtt cagagtaaca gtgcaggtga ctctgattta ccataacatc
     5101 tgttatcagc accgcctgag aggccctcag ggcgtttctg acgggaaacc ggtaaaacag
     5161 gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg
     5221 agtatgcgca caagtgcata gcgcggaaat tcacaccatt cagccggggt aagtcctgtt
     5281 tccggcgttc ttcccatccg aaaacggtga acggggacgc aatttattgc gtggccgtga
     5341 agcgttgccg gcgcagccgg aaaacccgct gtcagcacgt tcaggaaggg ggtgatgtac
     5401 aaatggcaca gaggatgaaa gtgacgtatg agctgaagga atgatgtaaa agtgatgtat
     5461 aagtgatgta aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa
     5521 taacccgttc atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc
     5581 ccttaacctg cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata
     5641 cacctaacgc agccgcgatt ttcatcgcaa ctgcatgaat taaaagaaaa aaaaggaata
     5701 tttcgttttc acgagtaata cccgggtatt aaaaatccac tgtaattaca tgaaaataaa
     5761 gaaataaaag gtaataccgg agtattacca cgtacgcggg caagcgaaaa agctataacg
     5821 cttcccggcc gacaggcgac ccaaaatcca caggggcatg caggttctct tcagtaacct
     5881 gcgccattaa atcaaaaagg tcatattcgc cgggaagctg cgtatcagtc gaatccagtt
     5941 gttcatcata ttcagatggc atggggtgat atccagtagc tgagaggttg ttagcgggtt
     6001 tcggggcgca gccctgaacc agtcatgtag cgctagcgga gtgtata
//
